Expression HLA-G gene and its correlation with IL-10 in tissue of cervical cancer

윤보성 ( Bo Sung Yoon ),( Young Tae Kim ),( Jae Wook Kim ),( Jae Hoon Kim ),( Sung Hoon Kim ),( Sang Wun Kim ),( Eun Ji Nam ) 대한산부인과학회 2006 대한산부인과학회 학술대회 Vol.92 No.-

중·고등학교 태권도 시범단원의 참여동기, 신체적 자기효능감, 학교생활 만족도의 관계

윤보성(Yoon, Bo-Sung),양대승(Yang, Dae-Seung) 한국체육과학회 2016 한국체육과학회지 Vol.25 No.1

This study aims to examine the participation motivation of the members in middle school and high school Taekwondo demonstration team and its following effects on physical self-efficacy and school life satisfaction to closely analyze the relationship betweeen them. For the data, 273 members of 4 middle school and high school demonstration teams and 6 Taekwondo gyms were randomly sampled from the full population of middle and high school Taekwondo demonstration teams and Taekwondo gym demonstration teams in GyeongGi-Do region. Exploratory factor analysis, calculation of reliability coefficient(Cronbach’s α), descriptive statistics, correlation analysis and stepwise multiple regression were conducted by using the statistics package SPSS 18.0 for the collected data. The conclusions of this study obtained through the data analysis following the above methods and procedure are as follows. First, internal motivation, which is a sub-factor of participation motivation of middle and high school Taekwondo demonstration team members, had positive impacts on self-esteem for physical selfrepresentation and recognized physical ability, whereas external motivation had negative impacts on self-esteem for physical self-representation and recognized physical ability. Second, internal motivation, which is a sub-factor of participation motivation of middle and high school Taekwondo demonstration team members, had stastically significant positive impacts on relationship with friends, relationship with teachers, learning activities, school events and rule compliance, which are sub-factors of school life satisfaction, whereas external motivation had statistically significant positive impacts on relationship with friends and negative impacts on relationship with teachers and learning activities. Third, physical self-efficacy of middle and high school Taekwondo demonstration tema members had statistically significant positve impacts on 5 sub-factors of school life satisfaction.

단일공법 복강경하 난소낭종절제술과 고식적 방법과의 비교

이윤정 ( Yoon Jung Lee ),김명주 ( Myung Joo Kim ),성석주 ( Seok Ju Seong ),김인현 ( In Hyun Kim ),김미라 ( Mi La Kim ),송태종 ( Taejong Song ),윤보성 ( Bo Sung Yoon ),정용욱 ( Yong Wook Jung ),주원덕 ( Won Duk Joo ),전혜선 ( Hye S 대한산부인과학회 2012 Obstetrics & Gynecology Science Vol.55 No.5

목적 양성 난소낭종절제술에서 단일공과 고식적 복강경수술의 결과를 비교하여 단일공수술의 유용성과 안전성을 알아보고자 하였다. 연구방법2010년 1월부터 2011년 7월까지 단일공 복강경하 양성 난소낭종절제술을 받은 28명을 진단명에 따라 3군(자궁내막종, 성숙기형종, 그외)으로 분류하여 각 1:2비율로 고식적 복강경하 난소낭종절제술 받은 56명을 선정하여, 후향적인 의무기록 분석을 시행하였다. 결과환자의 나이, 체질량지수, 기존의 복강내 수술 여부나 낭종의 양측성 및 낭종의 평균 크기는 두 군 간 유의한 차이가 없었다. 유착박리술여부, 동시에 시행한 수술 여부, 실혈량, 수술시간, 수술 전후 혈색소 수치변화, 추가 진통제 사용 여부, 재원기간의 수술 결과에 있어서 양군 간 유의한 차이가 없었다. 그러나 낭종 종류별 분석결과, 자궁내막증에서 실혈량의 중위값은 단일공군 30 mL (범위, 30-200 mL), 고식적 복강경군 100 mL (범위, 30-450 mL)로 유의한 차이를 보였고( P = 0.017) 수술시간 또한 고식적 복강경 군의 중위값 60분(범위, 40-85분)으로 단일공군의 40분(범위, 30-100분)보다 유의하게 길었다( P = 0.004). 성숙기형종에서는 수술시간, 실혈량은 두 군 간 유의한 차이가 없었으나 수술 후 혈색소 수치감소 중위값은 단일공군에서 2.3 g/dL (범위, 2.0-3.1 g/dL)로 고식적 복강경수술을 받은 군인 1.4 g/dL 범위, 0.1-1.8 g/dL)보다 의미 있게 컸다( P = 0.002). 결론 배꼽 절개를 통한 단일공 복강경수술은 양성 난소낭종절제술에 있어 안전하고 유용하며, 기존의 고식적 복강경하 수술의 대안이 될 수 있다. Objective This study was conducted to compare the surgical outcomes between single-port access (SPA) and conventional laparoscopic ovarian cystectomy. Methods This retrospective, matched case-control study was performed in 84 cases, who underwent SPA laparoscopic ovarian cystectomy (cases: 28) by a single surgeon and conventional laparoscopic ovarian cystectomy (control: 56) by another surgeon who had similar surgical skill at our hospital between January 2010 and July 2011. Results All procedures were performed successfully in both groups without conversion to explo-laparotomy. There was no significant difference in demographic characteristics including size of ovarian cysts, estimated blood loss (EBL), operation time, hemoglobin change, postoperative hospital stay and number of patients needed extra anti-infl ammatory drugs between two groups. Analysis according to the type of cysts, median EBL (30 mL vs. 100 mL, P = 0.017) was higher in conventional group and operation time (40 minutes vs. 60 minutes, P = 0.004) was also longer in conventional group in case of endometrioma. The EBL, median operation time and postoperative hospital days were similar in both group, but median hemoglobin change was less in conventional group (2.3 g/dL vs. 1.4 g/dL, P = 0.002) in mature cystic teratoma patients. There were no signifi cant difference of surgical outcomes in others group. Conclusion SPA laparoscopic ovarian cystectomy using conventional laparoscopic instruments is feasible, effective and reliable technique, which can substitute conventional laparoscopy.

탄소섬유 보강 (補强) 시멘트 복합체 (複合體) ( CFRC ) 의 건조수축 및 동결융해저항성에 관한 연구

박승범,이보성,윤의식 ( Seung Bum Park,Bo Sung Rhee,Eui Sik Yoon ) 한국복합재료학회 1989 Composites research Vol.2 No.1

자궁경부암에서 HLA-G와 IL-10의 발현 및 상호작용

김영태 ( Young Tae Kim ),윤보성 ( Bo Sung Yoon ),김재욱 ( Jae Wook Kim ),김성훈 ( Sung Hoon Kim ),김재훈 ( Jae Hoon Kim ),김상운 ( Sang Wun Kim ),남은지 ( Eun Ji Nam ) 대한산부인과학회 2007 Obstetrics & Gynecology Science Vol.50 No.1

목적: 본 연구의 목적은 자궁경부암 환자의 조직에서 HLA-G 유전자 및 IL-10 유전자의 발현을 reverse transcriptase- polymerase chain reaction (RT-PCR) 및 western blot analysis를 통해 알아보고, HLA-G와 IL-10 발현의 상관성과 자궁경부암의 임상병리학적 예후인자와의 연관성을 알아보는 것이다. 연구 방법: 2004년 10월부터 2005년 10월까지 자궁경부암으로 진단받은 40명의 환자와 정상환자 15명의 자궁경부 조직을 대상으로 하였다. HLA-G의 mRNA의 측정은 quantitative real time RT-PCR을 이용하였고, IL-10 mRNA의 경우에는 semi-quantitative RT-PCR을 이용하였다. 그리고 HLA-G 및 IL-10의 단백질 발현은 western blot analysis를 통해 측정하였다. 결과: HLA-G 및 IL-10의 mRNA 발현은 정상 자궁경부 조직에 비해 자궁경부암 환자의 조직에서 통계학적으로 유의하게 더 높게 나타났다 (P<0.001, P<0.001). HLA-G와 IL-10의 단백질 발현 또한 자궁경부암 조직에서 더 높은 발현을 보였다 (P<0.001, P=0.041). HLA-G의 mRNA 발현은 IL-10의 mRNA 발현과의 상관성은 통계학적으로 유의하지 않았지만, 상관성 경향을 보였다 (P=0.061). 자궁경부암의 임상병리학적 예후인자와의 연관성에서 HLA-G의 mRNA 발현은 FIGO 병기와 연관성이 있었으며, 초기 병기에서 HLA-G mRNA 발현 수치가 더 높게 나타났다 (P=0.045) 결론: 자궁경부암 조직에서 HLA-G와 IL-10의 발현은 대조군에 비해 더 높게 나타났으며, 이는 HLA-G와 IL-10이 자궁경부암 환자에서 종양의 악성변환과 연관이 있음을 시사해준다. 자궁경부암의 예후인자 중 FIGO 초기병기에서 HLA-G의 발현이 높게 나타나는 것으로 보아 HLA-G가 자궁경부암의 초기 암화과정과 연관이 있다고 사료된다. Objectives: To determine the expression of HLA-G and IL-10 and their correlation in tissue of cervical cancer, and to investigate the relationship between their expression and clinicopathologic factors in patients with cervical cancer. Methods: Tissue samples were obtained from 40 patients diagnosed with cervical cancer and 15 patients with normal cervix for control from Oct. 2004 to Oct. 2005. Quantitative real-time RT-PCR for HLA-G mRNA and semi-quantitative RT-PCR for IL-10 mRNA were used. And proteins of HLA-G and IL-10 were detected by Western blot analysis. Results: Both HLA-G and IL-10 mRNA expression in cervical cancer tissue were higher than normal control, which was statistically significant (P<0.001, P<0.001). The proteins levels of HLA-G and IL-10 in cancer group were also significantly higher than control (P<0.001, P=0.041). The mRNA expression of HLA-G tended to be correlated with IL-10 expression (P=0.061), although it was not statistically significant. Among clinicopathologic factors of cervical cancer, there was inverse relationship between FIGO stage and mRNA value of HLA-G (P=0.045). Conclusions: The mRNA and protein expression of HLA-G and IL-10 in cervical cancer were much higher than in controls. These results showed that HLA-G and IL-10 might have an important role of tumorigenesis in patients with cervical cancer. The levels of HLA-G and IL-10 seem to be correlated although it was not statistically significant. High HLA-G mRNA expression could be related in early tumorigenesis since it was associated with early stage cervical cancer.

산업폐기물을 이용한 건재용 고성능복합체의 개발 및 응용에 관한 실험적 연구

朴承範(Park, Seung Bum),李輔成(Rhee, Bo Sung),尹義植(Yoon, Eui Sik) 한국콘크리트학회 1991 콘크리트학회논문집 Vol.3 No.3

편광 민감 광결맞음 단층 촬영 장치를 이용한 자궁경부의 조기 진단(A Preliminary Study)

강진호,이상원,유지영,강문식,김법민,윤보성,김영태,조남훈,Kang, Jin-Ho,Lee, Sang-Won,Yoo, Ji-Yeong,Kang, Moon-Sik,Kim, Beop-Min,Yoon, Bo-Sung,Kim, Young-Tae,Cho, Nam-Hoon 한국광학회 2007 한국광학회지 Vol.18 No.5

자궁경부 상피이형성증(cervical dysplasia)은 세포핵/세포질(nuclear/cytoplasmic, N/C) 면적비의 변화를 포함하는 자궁경부 상피에서의 형태학적 변화를 일으킨다. 세포핵은 중요한 산란 원인중에 하나이기 때문에 N/C 면적비의 변화는 깊이에 따른 원형 편광장도를 반영한다. 따라서 우리는 편광 민감 광결맞음 단층촬영장치(polarization-sensitive optical coherence tomography, PS-OCT)를 이용하여 산란에 의해 발생하는 편광의 변화를 측정하였다. 자궁 경부 조직 샘플은 고등급 상피내종양(high-grade squamous intraepithelial, H-SIL)을 가진 한 명의 환자와 정상인 네 명의 환자에게서 얻어졌다. 우리는 자궁경부의 깊이에 따른 원형 편광의 유지 정도(degree of circular polarization, DOCP) 평균을 구하였고, 상피층의 깊이에서 최소 제곱 1차 선형 함수(least-square linear fit)를 사용하여 기울기를 획득하였다. 획득된 기울기를 가지고 DOCP의 변화율을 정량화 하였다. 그 결과 H-SIL에서의 DOCP의 감쇄가 정상 조직에서 보다 빠르게 나타남을 확인할 수 있었다. 이는 PS-OCT를 이용하여 산란 변화에 따른 DOCP의 변화율을 측정하는 것이 자궁경부상피 이형성증의 진단을 하는데 있어서 유용한 진단법이 될 수 있음을 의미한다. Cervical dysplasia induces morphologic changes in the cervical epithelium which involve changes in the nuclear/cytoplasmic (N/C) ratio. Since the nucleus is one of the significant scattering sources, the N/C ratio change reflects the degree of circular polarization (DOCP) with the depth of signals. Therefore, we used the polarization-sensitive OCT (PS-OCT) technique to measure the polarization changes caused by scattering. Cervical tissues were obtained from a high-grade squamous intraepithelial lesion (H-SIL) of one woman and from low normal women. We obtained the mean of the DOCP as a function of depth in the cervix and quantified the change ratio of the DOCP using slopes that were determined by linear fits in the epithelium layer. We found that DOCP of H-SIL decayed faster than that of normal tissue because of the higher scattering in H-SIL as expected. This result indicates that the PS-OCT system might be useful in measurements of change ratio of DOCP with depth for screening of cervical dysplasia.

자궁경부암에서 3차원 자기공명영상 디지털자료의 컴퓨터 가상기법을 이용한 예후인자의 개발

김영태 ( Young Tae Kim ),김상운 ( Sang Wun Kim ),남은지 ( Eun Ji Nam ),김성훈 ( Sung Hoon Kim ),김재훈 ( Jae Hoon Kim ),김재욱 ( Jae Wook Kim ),윤정섭 ( Joung Sub Youn ),윤보성 ( Bo Sung Yoon ) 대한산부인과학회 2007 Obstetrics & Gynecology Science Vol.50 No.10

복강경보조질자궁절제술과 복강경전자궁절제술의 수술결과 비교

박현 ( Hyun Park ),최민철 ( Min Chul Choi ),윤보성 ( Bo Sung Yoon ),성석주 ( Seok Ju Seong ),조연경 ( Yeon Kyung Cho ),신중식 ( Joong Sik Shin ),전혜선 ( Hye Sun Jun ),심정연 ( Jeong Yun Shim ),김인현 ( In Hyun Kim ),박종택 ( Cho 대한산부인과학회 2008 Obstetrics & Gynecology Science Vol.51 No.7

목적: 복강경보조질자궁절제술 (LAVH)과 복강경전자궁절제술 (TLH)의 수술 결과를 비교하고자 하였다. 방법: 병원기록을 바탕으로 2006년 9월부터 2007년 8월까지 복강경자궁절제술을 받았고 병리소견이 자궁근종 혹은 샘근육증인 경우만을 대상으로 하여 LAVH를 받은 93명과 TLH를 받은 142명을 선정하였다. 수술방법에 따라서 환자의 인구학적 특징과 수술결과를 비교하였다. 여러 변수들 사이의 관계를 알아보고자 다중회귀분석을 실시하였다. 결과: 두 군 간에 나이 및 체질량지수, 수술력, 병리소견은 차이가 없었다. 단변량 분석에서 LAVH의 수술시간 (129.0±34.5분, 123.6±40.8분)과 실혈량 (385.5±296.1 mL, 294.7±285.4 mL)이 증가하였다 (P<0.050). 검체무게는 LAVH에서 무거운 경향이 있었으며 (378.7±211.7 g, 316.9±170.1 g, P=0.060), 입원기간 (5.63±1.61일, 5.71±1.61일)과 부작용 발생률 (10.8%, 14.0%)은 차이가 없었다 (LAVH, TLH, P>0.050). 다변량 분석에서 실혈량은 수술시간 및 검체무게과 관련이 있었으며 (P=0.000), 수술방법과는 관련이 적었다 (P=0.213). 결론: TLH에 비하여 LAVH가 시행된 경우에는 자궁이 무겁고 수술시간이 길며 실혈량이 많은 경향이 있었다. 다변량 분석을 통하여 복강경자궁절제술에서 실혈량은 수술방법과는 관련이 없으며 검체무게 및 수술시간과 비례함을 알 수 있었다. Objective: To compare the surgical outcomes between laparoscopy-assisted vaginal hysterectomy (LAVH) and total laparoscopic hysterectomy (TLH). Methods: The data were collected retrospectively from hospital records. Between September 2006 and August 2007, Patients undergone with LAVH (93 cases) and TLH (142 cases) with pathologic reports of leiomyoma or adenomyosis were enrolled. The characteristics and surgical results were compared according to the operation type. The correlations among the variables were analyzed with multiple linear regression. Results: Between two groups, the characteristics of patients were similar such as age, body mass index, surgical history, and pathologic findings. In the univariate analysis, the differences of operation duration and blood loss between LAVH (129.0±34.5 min, 385.5±296.1 mL) and TLH (123.6±40.8 min, 294.7±285.4 mL) were significant (P<0.050). Specimen weight was slightly heavier in LAVH (378.7±211.7 g vs. 316.9±170.1 g, P=0.060), Admission duration (5.63±1.61 day vs. 5.71±1.61 day) and the incidence of complications (10.8% vs. 14.0%) were indifferent to the groups (LAVH vs. TLH, P>0.050). In multivariate analysis, blood loss was correlated with operation duration and specimen weight (P=0.000) but not with operation type (P=0.213). Conclusions: LAVH tends to be selected in larger uteri and results in more blood loss and longer operation duration. Operation type (LAVH or TLH) does not affect blood loss which is related with operation duration and uterine weight.

로봇 복강경하 전자궁절제술 한국최초 임상보고

김영태 ( Young Tae Kim ),김상운 ( Sang Wun Kim ),윤보성 ( Bo Sung Yoon ),남은지 ( Eun Ji Nahm ),허혜원 ( Hye Won Hur ),김성훈 ( Sung Hoon Kim ),김재훈 ( Jae Hoon Kim ),김재욱 ( Jae Wook Kim ) 대한산부인과학회 2006 Obstetrics & Gynecology Science Vol.49 No.12

Hysterectomy is one of the most common non-pregnancy-associated surgical techniques in the field of gynecology. Over the years, this laparoscopic approach has evolved to include both subtotal and total laparoscopic hysterectomy. Robot-assisted technology may attenuate the learning curve for complex laparoscopic procedures, leveling the playing field between conventional laparotomy practitioners and laparoscopists. The advantages of robotics, such as motion scaling, three-dimensional visualization, and articulated instrumentation, enables complex surgical procedures to be performed with greater dexterity, more quickly, and more easily by many surgeons. We have experienced a case of patient with carcinoma in situ of the uterine cervix treated by robotic surgery. We report the first case treated by robotic total laparoscopic hysterectomy with a brief review of literature.