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감마선에 대한 고구마 Peroxidase 형질전환 담배식물체의 반응
윤병욱,이행순,권석윤,김재성,곽상수 한국식물생명공학회 1999 식물생명공학회지 Vol.26 No.4
고구마 산성 peroxidase (POD) (swpal) 또는 중성 POD(swpnl)을 도입한 형질전환 담배에 다양한 선량의 감마선을 조사한 후 30일에 항산화효소 활성과 식물생장에 미치는 영향을 조사하였다. 감마선은 조사선량에 비례하여 형질전환 식물체와 정상식물에 관계없이 모든 식물체의 생장을 크게 억제시켰다. 50-70 Gy 처리에서 식물체의 신장이 크게 억제되었고 새로운 잎이 발생되지 못하였다. 10-50 Gy의 조사선량에서는 POD, superoxide dismutase, catalase 활성에 큰 변화를 주지 않았다. 이러한 결과는 고구마 POD는 감마선 조사에 의해 유도되는 산화적인 스트레스에 대한 보호효과에 관여하지 않음이 시사된다. Transgenic tobacco plants expressing either a sweet potato anionic peroxidase (POD) (swpal) or neutral POD (swpnl) were irradiated by gamma radiation, and the gamma radiation-induced biochemical changes in antioxidant enzymes and plant growth inhibition were investigated at 30 days after treatment. Gamma radiation significantly inhibited the growth of all plants regardless of transgenic or nontransformed plants, showing a dose-dependent inhibition. In high dosage of 50 and 70 Gy, plant heights were severely retarded and new leaves does not emerged. No significant changes in antioxidant enzymes such as POD, superoxide dismutase and catalase were observed in all plants regardless of irradiation dosages ranging from 10 to 50 Gy. These results suggest that sweet potato PODs may be not involved in the protection against the oxidative stress induced by gamma radiation.
윤병욱,이재홍,강주원,Yoon, Byoung-Wook,Lee, Jae-Hong,Kang, Joo-Won 大韓建築學會 2014 大韓建築學會論文集 : 構造系 Vol.30 No.8
Tensegrity structures are pre-stressed pin-joint structures inclusive tensile members and compressive members. Form-finding is very important for Tensegrity structures need to determine shape and pre-stress value for self-equilibrium. In the form-finding of tensegrity structures, Infomation are usually introduced for initial geometry and pre-determined member types. In this study, Form-finding of tensegrity structures by using genetic easy and versatile form-finding processing requires only a minimal information 'topology'. Genetic Algorithms was determined the type of members. The form-finding of tensegrity structures are found by a full iteration pocedure until a state of self-stress is obtained.
골신장술시 반복적인 압축과 신장 방법이 골형성에 미치는 영향
윤병욱,김여갑,오정환,Yoon, Byung-Wook,Kim, Yeo-Gab,Oh, Jung-Hwan 대한악안면성형재건외과학회 2008 Maxillofacial Plastic Reconstructive Surgery Vol.30 No.5
Purpose: The aim of the present study is to evaluate the effect of repetitive distraction and compression on new bone formation during distraction period. Materials and methods: Sixteen healthy rabbits, weighing about 2.5kg, were used in this experiment. A unilateral mandibular osteotomy was performed in the left mandible and the distractor(Track 1 $plus^{(R)}$, Gebruder Martin $GmbH^{(R)}$, Germany) was fixed with four screws (Cross driver screw $TI^{(R)}$, Gebruder Martin $GmbH^{(R)}$, Germany). After 4 days, the mandibles were distracted at a rate of 0.6mm/day for 10 days to obtain the amount of 6mm distraction in the control group(n=4). In the experimental group A(n=6), they were distracted at a rate of 1.2mm/day for 5 days and then compression of 0.6mm length and distraction of 0.6mm per 12 hours were carried out as counter direction for 5 days, relatively. In the experimental group B(n=6), distraction of 1.2mm length and compression of 0.6mm length per 12 hours were repeated for 10 days to obtain the amount of 6mm distraction finally. The experimental animals were sacrificed at 2 and 4 weeks after surgery and block specimens were obtained. With histologic and histomorphometric analysis, we observed the histologic changes of the cells and bone formation after H-E and Masson- Trichrome staining and then, measured Bone Deposition Rate with TOMORO $ScopeEye^{TM}$ ver. 3.5(Olympus, Japan), Results: Histologically, new bone formation was examined in all experimental groups and the control. But, the ability of bone formation of the experimental group A was somewhat better than any other groups. On the histomorphometric analysis, Bone Deposition Rate was higher in the experimental group A$(50.67{\pm}4.36%)$ than in the control group$(45.94{\pm}3.97%)$ and in the experimental group B$(42.68{\pm}5.70%)$. These data showed significant differences statistically(p<0.05). Conclusion: These results show that the distraction osteogenesis using repetitive compression and distraction force in the early consolidation period may be effective for new bone formation.
윤병욱,Yun, Byeong-Uk 한국주택협회 2006 주택과 사람들 Vol.192 No.-
그녀와 그, 해변에 있다면 바다의 수평선을 바라볼 것이고, 계곡에 있다면 자연의 풍요를 바라볼 것이다. 허니무너에게 따사로운 햇살과 살랑살랑 부는 바람, 그리고 결혼식을 준비하느라 쌓인 피로를 한번에 풀어줄 스파 서비스를 선사할 리조트 세 곳.
마이크로 유전알고리즘을 이용한 텐세그리티 모듈의 단면 및 형상 최적화
윤병욱,이재홍,Yoon, Byoung-Wook,Lee, Jae-Hong 대한건축학회 2011 대한건축학회논문집 Vol.27 No.9
This paper proposes size and shape optimization of Tensegrity structures by using micro-genetic algorithm. Tensegrity structures are general pin-jointed systems of discontinuous compressive members(Struts) and continuous tensile members(Cables). Form-finding process has been done before performing optimization of Tensegrity structures. The micro-genetic algorithm are employed for efficient optimum design. The object function for size and shape optimization is weight in all design constraints. The design constraints include stress and strength constraints, and cables and struts are applied as different design constraints. A few Tensegrity structure example is shown to demonstrate the efficiency and robustness of the proposed method.
대학도서관 장서점검 사례 : 세종대학교 학술정보원을 중심으로
윤병욱,김진 한국사립대학교 도서관협의회 2004 사대도협회지 Vol.5 No.-
세종대학교 학술정보원의 장서점검 사례를 통해 대학도서관의 장서점검에 있어서의 정보기술 도입 현황을 살피고, 장서점검 결과를 분석하여 향후 대학도서관의 운영과 정보서비스에 대한 방향을 제시했다.
레드 클로버의 엽육세포로 부터 원형질체의 라출 (裸出) 에 미치는 몇가지 요인의 영향
윤병욱,조진기,손대영 경북대학교 유전공학연구소 1995 遺傳工學硏究所報 Vol.10 No.1
An experiment was conducted to elucidated some factors affecting the isolation of protoplasts from red clover mesophyll tissues. Among the foot enzymes of Cellulase, Onozuka R-10, Macerozyme R-10, Driselase arid Pectolyase Y-23, the combination of 1% Cellulase R-10 and 0.5 5 Macerozyme R-10 gave the best result for the isolation of viable protoplasts from red clover mesophyll tissues. For the osmoticum, 6 concentrations of mannitol, 0.3-0.8 M were compared and 0.7 M brought the maximum protoplasts yield. The optimum enzyme solution pH for the protoplast isolation was 5.6 among the five treatments from 5.2-6.0. When red clover leaves were divided into four sizes, the larger leaves resulted in better yield of protoplasts. The sucrose concentration for purification of protoplasts isolated and to recover protoplasts from cell debris was 0.8 M and the recovery rate was 57 % at that concentration.