랫트의 간 손상에 대한 녹차카테킨의 보호 및 치료효과

육동연,이미애,윤여표 한국식품위생안전성학회 2004 한국식품위생안전성학회지 Vol.19 No.3

녹차카테킨은 다양한 생리활성을 지닌 것으로 알려지고 있다. 본 실험에서는 사염화탄소와 갈락토사민으로 유발된 간독성에 대한 녹차카테킨의 간기능보호효과가 연구되었다. 녹차카테킨(50 mg/kg와 100 mg/kg)은 사염화탄소(0.5 ml/kg)와 갈락토사민(400 mg/kg)이 투여되기 전 그리고 투여 후 3일동안 흰쥐에 경구투여되었고, 간기능지표로 AST와 ALT를 측정하였다. 녹차카테킨(50 mg/kg)은 사염화탄소 처리된 랫드에서 상승된 혈중 AST와 ALT활성을 전투여군(262±11, 80±19 에서 153±33, 55±25로)과 후투여군(156±40, 105±3에서 106±22, 55±9로)모두 감소시켰다. 또한 갈락토사민으로 유도한 경우에도 AST와 ALT 수치는 전투석군(576±24, 276±68에서 236±13. 115±13로) 후 투여군(233±54, 137±11에서 119±23, 44±17로)에서 모두 유의성있게 감소된 결과를 나타내었다 또한 간 조직학적 검사에서도 사염화탄소와 갈락토사민으로 유도된 간경변을 유의성있게 억제하였다. 이상의 결과로 볼 때 녹차카테킨은 간독성에 의한 병변을 예방 및 치료할 수 있는 신약후보물질로서의 가능성을 시사한다. Green tea catechin (GTC) is known to have a wide variety of pharmacological activities. In the present study, the effects of GTC on acute hepatotoxicity induced by carbon tetrachloride (CCI??) and galactosamine were examined in rats. Two doses (50 or 100 mg/kg) of GTC were administered to rats orally for 3 days before or after the induction of hepatotoxicity. A hepatotoxicity was induced by the intraperitoneal injection of the CCI₄ (0.5 ml/kg) or galactosamine (400 mg/kg). GTC (50 mg/kg) reduced the aspartate aminotransferase (AST) and alanine aminotransferase (ALT) level of the CCI₄,-intoxicated rats in the pre-treatment group (from 262±11, 80±19 to 153±33, 55±25), and also in the post-treatment group (from 156±40, 105±3 to 106±22, 55±9), respectively. And GTC (50 mg/kg) also reduced the levels of AST and ALT in both pre-treatment (from 576±24, 276±68 to 236±13, 115±13) and post-treatment (from 233±54, 137±11 to 119±23, 44±17) when induced by galactosamine. GTC also showed the inhibition of pathogenesis of hepatocyte of CCI₄- and galactosamine-intoxicated rats. These results suggest that green tea catechin (GTC) may be useful for the prevention and therapy of hepatotoxic pathogenesis.

구리시 협의회, 2015년 한마음축제의 밤

육동연 한국자치학회 2016 월간 주민자치 Vol.51 No.-

마우스에서 L-Theanine의 기억력 회복능 및 Acetylcholinesterase 활성 억제

육동연,김태일,박상기,박형국,윤여경,홍진태,Yuk, Dong-Yeon,Kim, Tae-Il,Park, Sang-Gi,Park, Hyoung-Kook,Yoon, Yeo-Kyeung,Hong, Jin-Tae 대한약학회 2007 약학회지 Vol.51 No.6

Acetylcholinesterase (AChE) plays a role in the progression of Alzheimer's disease (AD). In this study, we examined the improving effect of L-theanine, a major amino acid in Japanese green tea (Camellia sinensis) on the scopolamine (1 mg/kg/mouse)-induced memory dysfunction in mice. Treatment with L-theanine (2, 4 mg/kg/mouse p.o.) in the drinking water for 7 days reversed the scopolamine-induced latency time and distance in the water maze test, latency time in the passive avoidance test, and inhibited AChE activity. This study suggests that L-theanine may be a useful agent for prevention of progression of AD.

회원 학술보고 : 19. 유기음이온계 약물의 간내이행과정에 있어서 Cytoskeleton 의 역할에 관한 속도론적 연구

육동연(Dong Yeon Yuk),정연복(Youn Bok Chung),한건(Kun Han) 한국약제학회 1991 한국약제학회 총회 및 학술대회 요지집 Vol.- No.21

[2015년 경기도 주민자치 워크숍_분임토의 결과 발표] 주민자치 정책·사업·강좌별 필요한 지원 도에 건의

전성원,육동연,이강우,김현채,김동욱 한국자치학회 2015 월간 주민자치 Vol.49 No.-

녹차추출물/L-Theanine 혼합물의 Secretase 활성 억제 및 세포사 억제를 통한 기억력 회복능

김태일,육동연,박상기,박형국,윤여경,홍진태,Kim, Tae-Il,Yuk, Dong-Yeon,Park, Sang-Ki,Park, Hyoung-Kook,Yoon, Yeo-Kyeung,Hong, Jin-Tae 대한약학회 2008 약학회지 Vol.52 No.5

We examined the effect of green tea extract (GTE) and L-theanine combination on the $A{\beta}_{1-42}$-induced memory dysfunction. GTE and combination were administrated into mice for 3 weeks followed by injection of $A{\beta}_{1-42}$ to induce memory impairment. GTE and L-theanine administration significantly improved cognitive ability and reduced $A{\beta}_{1-42}$ level accompanied with the inhibition of neuronal cell death and activities of secretase. These results suggest that GTE and L-theanine combination may be a useful for preventing for the development or progression of Alzheimer's disease.

유리간세포를 사용한 ANS 의 간내 이행에 관한 연구 : ANS 의 간내 이행과정에 단백질 매개 기구가 존재하는가 ?

한건,정연복,육동연 한국약제학회 1991 Journal of Pharmaceutical Investigation Vol.21 No.1

The hepatic uptake of an anionic fluorescence probe, 1-anilino-8-naphthalene sulfonate (ANS) was characterized using isolated rat hepatocytes. The initial uptake rate of ANS by isolated hepatocytes was determined. The uptake process of ANS was fitted well to the Michaelis-Menten equation with a saturable component. The V_(max) and K_m values were 2.9±0.1 n㏖/min/㎎ protein and 29.1±3.2 μM, respectively. The uptake clearance (CL_(up)) based on the ratio of V_(max) to K_m was 11.7 ㎖/min/g liver, revealing the good coincidence with that assessed from the analysis of the plasma disappearance curve in previous report. Forthermore, the effect of serum protein on the hepatic uptake of ANS into isolated hepatocytes was investigated. The permeability clearances (PS_(inf)) of ANS uptake were much higher than those predicted based on the unbound fractions in the presence of serum. These suggested that the hepatic uptake of extensively serum protein-bound ANS is mediated not only by the unbound form of ligand but also by the serum protein-mediated uptake mechanism.

유기 음이온계 약물의 간내 이행과정에 있어서 Cytoskeleton 의 역할에 관한 속도론적 연구

한건,정연복,육동연 한국약제학회 1992 Journal of Pharmaceutical Investigation Vol.22 No.1

The effects of colchicine on the plasma elimination and biliary excretion of various organic anions in rats were examined. Elimination of indocyanine green (ICG) or rose bengal (RB) from plasma was significantly delayed when rats were treated with colchicine (3 ㎎/㎏ body weight) 3 hr prior to the administration of the dye. On the other hand, disappearance of sulfobromophthalein (BSP) or bromophenol blue (BPB) from plasma was not influenced by colchicine. The plasma disappearance and biliary excretion of organic anions were kinetically analyzed based on a compartment model, in which the deep compartment and the unknown disposition are incorporated. The transfer rate constants of ICG or RB, k_(23) (from the liver to the deep compartment) and k_(3B) (from the deep compartment to the bile), were decreased by colchicine, but those of BSP or BPB were not changed. A mechanism for the decrease in the k_(23) and k_(3B) values for ICG and RB might be explained by a inhibition of colchicine to the intracellular cytoskeleton. The hepatocellular distribution of RB or BPB was then determined. BPB mainly distributed to the cytosolic fraction, but RB distributed to each hepatocyte organelle. Taken together, it was suggested that ICG or RB is transported through hepatocytes into bile with the aid of the cytoskeleton, whereas BSP or BPB is handled by hepatocytes in a different way.

유기 음이온계 약물의 간내 이행과정에 있어서 Cytoskeleton의 역할에 관한 속도론적 연구

정연복,한건,육동연,Chung, Youn-Bok,Han, Kun,Yuk, Dong-Yeon 한국약제학회 1992 Journal of Pharmaceutical Investigation Vol.22 No.1

The effects of colchicine on the plasma elimination and biliary excretion of various organic anions in rats were examined. Elimination of indocyanine green (ICG) or rose bengal (RB) from plasma was significantly delayed when rats were treated with colchicine (3 mg/kg body weight) 3 hr prior to the administration of the dye. On the other hand, disappearance of sulfobromophthalein (BSP) or bromophenol blue (BPB) from plasma was not influenced by colchicine. The plasma disappearance and biliary excretion of organic anions were kinetically analyzed based on a compartment model, in which the deep compartment and the unknown disposition are incorporated. The transfer rate constants of ICG or RB, $k_{23}$ (from the liver to the deep compartment) and $k_{3B}$ (from the deep compartment to the bile), were decreased by colchicine, but those of BSP or BPB were not changed. A mechanism for the decrease in the $k_{23}$ and $k_{3B}$ values for ICG and RB might be explained by a inhibition of colchicine to the intracellular cytoskeleton. The hepatocellular distribution of RB or BPB was then determined. BPB mainly distributed to the cytosolic fraction, but RB distributed to each hepatocyte organelle. Taken together. it was suggested that ICG or RB is transported through hepatocytes into bile with the aid of the cytoskeleton, whereas BSP or BPB is handled by hepatocytes in a different way.

用途地域制의 法的 性質에 관한 考察

송현담(Song, Hyun-Dam),육동연(Youk, Dong-Youn) 한국토지공법학회 2012 土地公法硏究 Vol.56 No.-

用途地域制는 도시관리계획의 하나이다. 도시관리계획의 법적 성질에 대하여 법규범이냐 아니면 행정행위이냐는 것은 중요한 의미를 갖는다. 판례의 입장은 도시관리계획은 구속적 계획으로서 處分性을 인정하는 태도를 취하고 있다. 그러나 도시관리계획이 行政行爲로서의 성질을 갖는다 하더라도 取消訴訟을 제기하여 權利救濟를 받기 어려운 한계가 있다. 이는 행정청에게 計劃裁量이라는 광범위한 形成의 自由가 인정되고 있기 때문이다. 대법원은 形成의 自由는 무제한적인 것이 아니라고 하면서 利益衡量의 탈락․흠결 또는 利益衡量상의 평가 과오의 경우를 裁量權을 逸脫․濫用한 것으로 판시하고 있다. 판례가 학설상의 衡量命令과 衡量瑕疵및 計劃裁量이념을 채택하였다는 점은 평가할수 있으나, 衡量命令의 법적 근거에 대해 판시를 하지 않은 점과, 衡量瑕疵용어를 결여한채, 그것을 裁量權의 逸脫․濫用으로 이해하는 판시를 한 것은 논리상 문제가 있다. 計劃裁量의 경우 衡量瑕疵그 자체가 위법이 원인이 되며, 따라서 衡量瑕疵의 輕重에 따라 결과로서의 行政計劃이 無效또는 取消事由를 지닌 것으로 평가되어야 하는 것이다. Land use zoning is one of City Management Plan (CMP). There has been a debate on what is a legal nature of CMP, because CMP has both sides of legal status at the same time; a legal norm and/or an administrative act. The court has taken a stance that CMP is an administrative act, because CMP is a kind of restrictive plan which allows the government to have a administrative disposition. If CMP is an administrative act, the private rights violated by CMP can be remedied through an administrative suit for revocation. However, in the real world, the violated private right cannot be easily remedied because the government's discretionary power on CMP has been recognized very widely as a freedom of formation. The Supreme Court had decided that the government's freedom of formation shall not be an unlimited power and thus the government's discretionary power shall be deviated or abused when there are deviation, defect, or mis-calculation of profit-loss measurement of government's discretionary power. It is meaningful that the judicial precedent had adopted the academical reasoning regarding to profit-loss measurement on administrative act, defects of profit-loss measurement, and discretionary power on land use planning. Nevertheless, the judicial decision seems to have some limitations such as the court did not mention the legal basis of ordering profit-loss measurement and the definition of defects of profit-loss measurement when the court decided deviation, defect, or mis-calculation of profit-loss measurement of government's discretionary power. In the case of discretionary power on land use planning, the defects of profit-loss measurement itself can be the cause of illegal administrative act. In such a case, the administrative planning shall be invalidated or cancelled pursuant to the gravity of the defects of profit-loss measurement.