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유찬,윤용철,안병관,옥정훈,Yu Chan,Yoon Yong Cheol,Ahn Byung Kwan,Ok Jung Heun 한국관개배수위원회 2004 한국관개배수논문집 Vol.11 No.2
In this study, the runoff characteristic of nutrients in the small sized stream was investigated with seasonal change. The area considered was greenhouse site situated near Nam river, Jinju, Korea. In this site, groundwater was used for the irrigation and
사질토 간극수의 이온 성분들에 따른 전기비저항값의 변화
유찬,윤춘경,이영남,이용길,Yu, Chan,Yoon, Chun-Gyeong,Lee, Young-Nam,Lee, Yong-Gil 한국지반공학회 1999 한국지반공학회논문집 Vol.15 No.1
실내실험에 의하여 사질토 간극수의 이온 성분들사이 관계를 규명하기 위하여 흙의 비저항측정 상자와 STING-Rl비저항 측정기를 이용하여 분석을 수행하였다. 실험은 이온성분들의 농도를 변화시키면서 실시하였다. 또한 2~3가지 성분을 혼합한 경우에 대해서도 비저항값의 변화를 측정하였다. 실험결과는 성분중에서 비소(As)의 비저항값의 감소가 가장 컸으며, 나머지 성분들은 일반성분들과 큰 차이를 보이지는 않았다. 또한 혼합성분의 경우에는 비저항값의 범위가 간극수에 포함된 성분들의 비저항값에 따라 달라지는 것으로 나타났다. A laboratory experiment was performed to examine the relationship between resistivity and ionic components in the pore water of a sand by using soil resistivity test box and STING-Rl. The resistivity measurement was performed with the concentration changes of ionic components. Also, the resistivity change was evaluated for multiple components. The results showed that the resistivity of Arsenic was less than other heavy metals. In the case of complex components, resistivity ranges depended on the resistivity of components existed in the pore water.
유찬,이선희,박배근 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2009 動物醫科學硏究誌 Vol.16 No.1
A 4-year-old, intact male, Jindo-dog was admitted to the Veterinary Medical Teaching Hospital of Chungnam National University with the history of diarrhea several times a day the onest of which was a month ago and weight loss. She was not prevented against intestinal parasites and heartmorm since 1 year ago. On physical examination, she was emaciated and had abdominal tension. A Giardia kit test indicated negative result. Results from a serum biochemical profile and electrolytes demonstrated mild reduction in phosphate and sodium. Microfilaria was detected on direct blood smear. A Heartworm kit test indicated positive result. Thoracic radiographs revealed dilation of pulmonary vessels, increased opacity of caudal lung field, and reversed D sign. Abdominal radiographs revealed loss of abdominal serosal details. A abdominal ultrasonograph showed the following: dilation of the intestinal lumen, hyperechoic linear material with acoustic shadowing in bowel, and enlarged lymph node. Fecal examination using saturated saline flotation method indicated that she had trichuris vulpis infection. Anthelmintics were administered orally, and antibiotics were administered parenterally. In addition, heatworm treatment was advised. She had no significant clinical signs such as diarrhea after ten days and five months.
유찬 한국농공학회 2003 한국농공학회논문집 Vol.45 No.6
Laboratory experiments were carried out to investigate the influence of pore water contamination on the treatment effect of sandy soil which was solidified by Portland cement. In the experiments, setting time of hydraulic cement that was mixed with contaminated mixing water was measured using Vicat equipment and observed the tendency of setting process with the kind of contaminants, organic or inorganic components. It was shown that organic contaminants of the mixing water affect largely on the initial setting process of hydraulic cement and inorganics, expecially heavy metals, did not affect on the initial setting process, otherwise it was appeared that setting time of the sandy soil that was contaminated with inorganic components was apparently faster than the sandy soil that did not include inorganic components even though organic concentrations was relatively low level (COD=200∼300) in the mixing water. The results of unconfined compression strength test (UCST) were well consistent with the results of Vicat equipment test.
토양의 세립분 함량과 염분농도가 TDR 측정값에 미치는 영향 고찰
유찬,이근후,Yu, Chan,Lee, Geun-Hu 한국관개배수위원회 2006 한국관개배수논문집 Vol.13 No.2
Experimental laboratory tests were carried out to assess the effect of fine content and salinity of soils on the measurement of TDR(Time Domain Reflectometry). In the test, using soil columm which was made by PVC pipe with the dimension of 25cm height and 20cm diameter, the salinity variation of soil was controlled by the solution which was dissolved NaCl to destilled water in the range of 0-40g.$L^-1$. The fine content of soil was controlled by kaolinite which was mixed with Jumunjin sand in the range of 0-50% to the total dry weight. The water contents of soil tested were measured with the conventional oven dry method beside TDR and compared the these values to figure out the extent of effect. As the results of tests, it was appeared that the water content measurement by TDR can be affected by the salinity level, fine contents, and the degree of saturation of the soil.
증발산 원리를 이용한 매립장 최종 복토공법의 복토재로서 석탄재와 인산석고의 환경적 영향 평가
유찬,양기석 한국지반환경공학회 2005 한국지반환경공학회논문집 Vol.6 No.1
In this study, the utilization of coal-ash and phosphogypsum was considered as the evapotranspiration final landfill cover(ET cover) material. Cover material considered was the mixture of the weathered granite soil, coal-ash and phosphogypsum and so we sequentially performed the leaching test, column test and field model test to investigate the environmental effects of mixtures of coal-ash and phosphogypsum. In the leaching test, all materials had lower heavy metal concentration than the regulated threshold values. The column test and the review of related regulations were carried out to determine the optimum mixing ratio(OMR) and OMR was soil(4):coal-ash(1): phosphogypsum(1) on the volume base, which was applied to field model test. Field model tests were continued from February to June, 2004 in the soil box that was constructed with cement block. It was verified that coal-ash and phospogypsum mixed with soil was safe environmentally and the mixture of both wastes could improve the water retention capacity of cover materials. 본 연구에서는 증발산 원리를 이용한 최종복토층 공법의 재료로서 석탄회와 인산석고의 활용성에 대해서 고려하였다. 복토재료는 일반 화강암질 풍화토에 석탄회와 인산석고를 혼합하는 것으로 하고 이에 따른 환경적 영향을 예측하기 위해서 실내용출시험, 컬럼실험, 현장 모형실험을 순서적으로 실시하였다. 용출실험 결과에서 각 혼합물의 중금속 함유량은 규정값 이하로 나타났으며, 관계규정과 컬럼실험의 결과를 컴토하여 재료간 최적 배합비율은 토양(4):석탄재(1):인산석회(1)로 결정하여 현장모형실험에 적용하였다. 환경적 평가를 위한 현장모형실험은 2004년 2월에서 6월까지 진행하였는데, 실험기간 중 혼합 복토재와 우수에 의해 발생되는 침출수의 관찰결과에서는 본 연구에서 고려한 혼합재료가 환경적으로 안전하게 사용될 수 있는 것으로 나타났다.
Development of In-Situ Soil Gas Monitoring Well for Managing the Bioventing Performance
유찬,Yu, Chan The Korean Society of Agricultural Engineers 2007 한국농공학회논문집 Vol.49 No.1
Bioventing is commonly used for petroleum hydrocarbon (PHC) spills. This process provides better subsurface oxygenation, thus stimulating degradation by indigenous microorganisms. Therefore soil vapor monitoring points (VMPs) are extremely important in determining the potential effectiveness of bioventing and in long-term monitoring of bioventing progress. In this study in-situ soil gas monitoring well (GMW) was developed and presented the pilot test results which recover the contaminated site by bioventing method. The result of application was successful and it was expected that GMW developed could be applied to the evaluation procedure of bioventing effectiveness and long-term remediation potential.
생장 및 부상성이 향상된 남세균 돌연변이 균주 Arthrospira platensis M20CJK3
유찬,김충재,최강국,안치용,최종순,오희목,Yoo, Chan,Kim, Choong-Jae,Choi, Gang-Guk,Ahn, Chi-Yong,Choi, Jong-Soon,Oh, Hee-Mock 한국미생물학회 2009 미생물학회지 Vol.45 No.3
A photosynthetic cyanobacterium Arthrospira platensis, well known for health food supplement, was studied as a target species for atmospheric $CO_2$ removal as well as biomass production. Although the biomass of A. platensis was massively produced in many countries, the recovery cost of its biomass is still high. The purpose of this study was to develop the A. platensis mutant strains which have enhanced growth rate and floatation activity to reduce the recovery cost. A. platensis KCTC AG20590 was treated with 0.24% ethyl methanesulfonate (EMS) for 20 min at room temperature. The mutant strain A. platensis M20CJK3 was finally selected by its morphological and physiological features. The morphology of the mutant A. platensis M20CJK3 was changed from loose-coiled form to tight-coiled form showing short pitch. The growth and $CO_2$ uptake rate of A. platensis M20CJK3 were improved about 15% and 17% compared with A. platensis KCTC AG20590, respectively. The floatation activity of A. platensis M20CJK3 was enhanced in 2-fold compared with that of A. platensis KCTC AG20590. Soluble proteins extracted from two strains were analyzed by two dimensional electrophoresis (2-DE) and MALDI-TOF MS/MS. Among 15 protein spots induced in 2-DE analysis, two spots were the proteins related to photosynthesis and electron transfer system of the other cyanobacteria. As a consequence, it seems that the tight-coiled mutant A. platensis M20CJK3 has an advantage of high growth rate and floatation activity which are beneficial for the mass cultivation and recovery. 건강보조식품 등으로 이용되는 Arthrospira platensis는 세계적으로 대량생산되고 있으나 생산공정 중 수확단계에서 많은 비용이 소요된다. 본 연구에서는 부상을 이용한 효과적인 수확을 위해 균주의 개량을 시도하였으며, 개량균주의 생리적 물리적 특성을 파악하고자 하였다. Ethyl methanesulfonate (EMS)를 모균주 A. platensis KCTC AG20590에 0.24%의 농도로 10, 20, 30분씩 처리하여, 형태 및 부상성이 우수한 균주 A. platensis M20CJK3 균주를 분리하였다. A. platensis M20CJK3은 느슨한 형태에서 촘촘한 형태로 세포사(trichome)의 길이 및 코일간 간격이 감소하였으며, 생장 및 $CO_2$ 고정능이 각각 15%, 17% 향상되었다. 또한, 개량균주의 부상성은 모균주에 비해 2배 이상 향상되었다. 이차원 전기영동 분석을 통해 모균주와 개량균주의 단백질 발현양상을 비교분석한 결과 광합성 관련 색소의 구조와 광전자전달계에 관련된 단백질의 발현 양상이 차이를 보였다. 본 연구에서 개발된 A. platensis M20CJK3은 고밀도 대량배양 및 수확에 유리하며, A. platensis 유전자 연구의 유용한 모델 균주로 활용될 수 있을 것으로 사료된다.