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노화촉진마우스의 기억력 및 산화 스트레스에 미치는 영지 (Ganoderma lucidum) 추출물의 영향
유제권,최선주,강종구,한상섭 한국생명과학회 1999 생명과학회지 Vol.9 No.5
Long-term effects of Ganoderma lucidum (GL) on memory and oxidative stress of senescence-accelerated mice (SAM) were investigated. Senescence-resistant (R1) and prone (P8) strains of SAM were fed GL diets, premixed with low (20 mg/kg/day, T1) or high (200 mg/kg/day, T2) levels of GL powder for 9 months starting from young (3 months of age) or for 5 months starting from old (7 months of age). After the final feeding at 12 months of age, all animals were subjected to passive avoidance test for the evaluation of memory function. In addition, the changes in hepatic thiobarbituric acid-reactive substance (TBARS) and glutathione were analyzed. SAMP8 fed GL diets from old age (7 months) exhibited the improvement of memory, although GL rather inhibited the memory function of both SAMR1 and SAMP8 mice fed diets from young (3 months of age). Hepatic TBARS contents were decreased in SAMP8 fed high GL diet for 9 months and in SAMR1 fed low GL diet for 5 months. Hepatic glutathione content was also remarkably increased in SAMR1 following both feeding periods, and less extent in SAMP8 fed diet for 5 months of age. Taken together, it is proposed that GL extracts may play an anti-aging role through antioxidant action, and thereby may improve the senescence-related memory dysfunction.
기억. 학습장애 동물모델 SAMP8에 미치는 알로에(Aloe vera)의 영향 II. SAMP8의 지질대사에 미치는 알로에의 투여효과
최진호,김동우,유제권,한상섭,심창섭,Choi, Jin-Ho,Kim, Dong-Woo,Yoo, Je-Kwon,Han, Sang-Sub,Shim, Chang-Sub 한국생명과학회 1996 생명과학회지 Vol.6 No.3
Aloe(Aloe vera LINNE) has been used as a home medicine for the past several thousand in the world, and has been studied on various chronic degenerative diseases such as atherosclerosis, myocardiac infarction and hypertension. SMAP8, learning and memory impairment animal mode, were fed basic or experimental diets with 1.0% of freeze dried(FD)-Aloe powder for 8 months. This study was designed to investigate the effects of Aloe on body weight gain, grading score of senescence(GSS), triglyceride, total and LDL-cholesterol levels, and atherogenic index in serum of SAMP8, and also designed to investigate the effects of Aloe on cholesterol accumultions in mitochondria and microsome fractions of SAMP8 brain. Body weight gain was consistently lower in aloe group than in control group, but no significantly differences between them. Grading score of senescence resulted ina marked decreases pf 20% in 1.0% Aloe group compared with control group. Administrations of 1.0% aloe resulted ina marked decreases in 15% and 20% of triglyceride and cholesterol levels, respectively, and also significantly decreased in 15% of LDL-cholesterol levels and atherogenic index in serum of SAMP8 compared with control group. Cholesterol accumulations were significantly inhibited in 20% and 10% of mitochondria and microsome fractions of SAMP8 brain, respectively, by administration of 1.0% Aloe. These results suggest that administration of Aloe mau not only effectively inhibit chronic degenerative diseases in serum of SAMP8, but may also improve learning and memory impairments of SAMP8 brain.
Effects of Storage Buffer and Temperature on the Integrity of Human DNA
김윤태,최은희,손보경,서은희,이은경,유제권,하기원,김진선,권미란,김영진,이경율 대한임상검사과학회 2012 대한임상검사과학회지(KJCLS) Vol.44 No.1
In this study, we have examined the effects of the storage time and temperature on DNA quality and have also studied the effects of the hydration buffer in which DNA is dissolved. This study was performed using 160 human blood samples collected with informed consent from 2007 to 2008 in the hospital where this cohort study was performed. The DNA extracted was dissolved using distilled water (DW) or Tris-EDTA (TE) buffer, and stored in the deep freezer or refrigerator for up to 10 weeks at -70℃, -20℃, 4℃, and 25℃, respectively. DNA integrity was determined by the degree of smearing of DNA on the gel. After four weeks, all of the 20 DNA samples dissolved in DW and stored at 25℃ were entirely degraded. After 10 weeks, 6 of the 20 DNA samples dissolved in TE buffer and stored at 25℃ were fairly degraded, and 4 of the 20 DNA samples dissolved in DW and stored at 4℃ were fairly degraded. The 20 DNA samples dissolved in TE buffer and stored at 4℃ were stable for 10 weeks. DNA samples stored at -20℃ and -70℃ did not appear to degrade in either DW or TE buffer, even at the 10-week point. We suggest that TE buffer should use for DNA elution, in order to protect against degradation and to preserve DNA for a long period of time, and the samples should be stored at -20℃ or -70℃. .