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Trypsin binding studies showed that sea crab α-macroglobulin (α-M) could bind 0.3 ㏖ of trypsin per molecule. It can, therefore, be concluded that the molecular ratio of proteinase/inhibitor was different from the stoichiometry for human α₂-M (half ㏖ecule) and also different from other data for the southern grass frog and American lobster where the values were in the range of 0.45∼0.79 ㏖ of trypsin/㏖ of macroglobulin, indicating the lower animals showed low trypsin binding capacity compared with that of higher animals. The a-M of the sea crab completely lost its ability for inhibition of trypsin activity by methylamine. The analysis of SΔQ parameter based on the amino acid compositions indicated that the sea crab α-M is closely related with that of the frog (Rana pipiens) and the horseshoe crab, respectively.
In case of KOREA, many difficulties are expected for pilots to adapt to the flight operational environment changes which we will meet suddenly in anytime of the future as the operating plan for GPS is not fully prepared yet. It is because GPS operation is rather difficult compared to other navigation equipments, and it takes much time and experience to be familiar with GPS instrument approach procedure First of all, it is keenly required tu establish GPS instrument approach procedure for the local airports to be familiar with GPS instrument approach procedure. We would like to introduce GPS instrument approach procedure for the Cheju International Airport as a basic model to help you to understand It.
남한사회에 유입된 북한이탈주민의 수가 급증하고 있고, 그동안의 지원 대책에 대한 문제점이 그들에게 현실적으로 사회 적응에 적절한 수준이 되지 못하고 있다는 지적이 자주 제기되고 있다. 북한이탈주민의 한국사회 정착에 대한 지원은 통일에 대비한 과정의 하나이다. 그리고 그 과정의 출발점은 우리 사회가 그들을 하나로 인정을 하는가 그렇지 못하는가와 관련이 있다. 만약 이 문제가 해결되지 못할 경우 북한이탈주민의 정착 문제는 새로운 남남갈등을 유발할 가능성마저 존재한다고 본다. 북한이탈주민에 대한 지원은 작은 부분부터 이루어져야 한다. 따뜻한 말 한마디와 배려가 그들의 마음을 훈훈하게 만들 것이기 때문이다. 차별은 자존심을 짓밟는다. 이러한 기본적인 노력을 시작으로 정부와 지역사회 차원에서의 지원이 이루어질 때 북한이탈주민의 진정한 한국사회 정착은 제 자리를 잡을 수 있을 것이다. The Number of North Korean Refugees is increasing rapidly. The Support of Korean government for them still has a lot of problems. The support for North Korean refugee has to accomplished small part. The start of support is related to if we recognize they are one of our part or not. If we recognize they are different to us, the process of the North Korean Refugee's settlement would bring about a new social conflict(south-south conflict.
The purpose of this study is to develop the test instrument for measuring the inquiry skill of middle school students. We reviewed other tests for measuring science inquiry skill and tried to develop the test instrument for measuring the skills of students. This study provides a test instrument using writing evaluation of student, which might be directly applied at school. To develop the test instrument for science inquiry we selected 10 possible elements among the inquiry process. We developed total 30 questions, three questions per each elements. The validity of each items and the objectivity of developed test were verified in two field trials and revised according to the analysis of items by six experts. The test instrument was conducted in field trial for the middle school students of 2nd year. According to the results of the field trial, the level of the reliability was proved 0.83, item difficulty 56.6%, level of item discrimination 0.39. Therefore, these results show that the developed instrument in this study is regarded as a appropriate instrument for evaluating science inquiry shills of middle school students.
Subunit heterogeneity of Portunus trituberculatus hemocyanin was investigated using various electrophoretic systems. SDS polyacrylamide gel electrophoresis (PAGE) was performed to investigate size homogeneity of hemocyanin. The electrophoretic pattern of purified hemocyanin with detergent, SDS in 7.5% gel revealed a single band with a molecular weight of about 75,000. The purified hemocyanin was then subjected to alkaline electrophoresis without detergent. Discontinuous-PAGE of hemocyanin in 5% gel showed single monomer band and higher polymeric bands. Discontinuous-PAGE containing 1 mM EDTA in gel and sample, however, showed two monomeric bands, indicating that the component of hemocyanin might be heterogeneous one. Discontinuous-PAGE in 7.5% acrylamide concentration showed at least four monomeric bands. In order to confirm the electrophoretic heterogeneity of Portunus trituberculatus hemocyanin subunits, continuous-PAGE was performed. Continuous-PAGE in a dissociating buffer (Tris/HCI-EDTA buffer pH 8.9) showed that monomeric subunits of Portunus trituberculatus hemocyanin were resolved into four distinct bands, designated as I, II, III, and IV, which agreed with the results of Discontinuous-PAGE in 7.5% gel.
This study focuses on the establishment of compulsory license to the patented invention, particularly pharmaceutic patent for public interests. With a view to promoting the development of technology and industry, Patents Act grants patentees an exclusive right to exploit the patented invention. For the purpose of the same goal, on the other hand, Patents Act imposes a few limits upon the exclusive right of patentees. A compulsory license is one of the limits upon patent right. A compulsory license is not an exceptional management from outside, it already exists in the inside of the right in view of the fact that Patent is the right granted artificially to contribute to the development of the industry. Pharmaceutic patent is closely related to human life directly or indirectly. Accordingly it has been discussed internationally to establish compulsory license to pharmaceutic patent for public interests. Regarding this problem, advanced countries and undeveloped countries are at issue with each other. We examine our position about compulsory license to pharmaceutic patent considering the degree of industrial development, especially the reality of pharmaceutic industry where Multinational Corporation of foreign country produces the most medicine. In case of Korea, there is a necessity for compulsory license in wide ranges in comparison with advanced countries such as Canada, Britain, Germany, France and so on within the limits of Agreement on TRIPs. In view of the above statements, it raises several problems of compulsory license under the Patent Act of Korea and proposes their solution. First of all there needs the realistic interpretation of the requirements for public interests and the flexible viewpoint about compulsory license to pharmaceutic patent. It is finally submitted that statutory provisions on compulsory license are designed as an efficient means of promoting goals of the Patents Acts and, also, that terms, conditions and procedures for compulsory license will have to be amended to make a better balance between the interests of patentees and public interest.