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유명조,고조 후지사끼,You, Myung-Jo,Fujisaki, Kozo The Korean Society of Veterinary Science 2003 大韓獸醫學會誌 Vol.43 No.1
To determine effectively the chitinolytic activity of rCHT1 from the hard tick H. longicornis expressed in baculovirus-mediated Spodoptera frugtperda (Sf) 9 cells, a simple and sensitive assay system was established in solid phase using agarose gel containing ethylene glycol chitin as substrate. The various factors affecting the efficacy of the assay were also investigated. The effects of various temperature, dosages of proteins, pH of media and time courses of reaction were examined to verify the sensitivity of assay for chitinolytic activity of rCHT1 protein. It was found that the optimal reactive conditions were $37^{\circ}C$ of temperature, 12 to 15 hours of reactive times, $0.1{\mu}g$ of protein concentration and pH 5 to 7 of media. Using the assay system designed, the functional activities of H. longicornis rCHT1l protein could be evaluated simply and sensitively.
유명조,고조 후지사끼,You, Myung-Jo,Fujisaki, Kozo The Korean Society of Veterinary Science 2003 大韓獸醫學會誌 Vol.43 No.1
A chitinase cDNA named CHT1 was cloned from the hard tick, Haemaphysalis longicornis, and the enzymatic properties of its recombinant proteins were characterized. The CHT1 cDNA encodes 930 amino-acid (aa) residues including a 22 aa putative signal peptide, with the calculated molecular mass of the putative mature protein 104 kDa. The E coli-expressed rCHT1 exhibited weak chitinolytic activity against $4MU-(GlcNAc)_3$. The rCHT1 protein with higher activity was obtained using recombinant Autographa californica multiple nuclear polyhedrosis virus (AcMNPV), which expresses rCHT1 under polyhedrin promoter. These findings suggest that the rCHT1 expressed in baculovirus-mediated Sf 9 cells has a high activity than E coli-expressed rCHT1.
개의 전침마취가 Vital sign 및 혈액가스치 변화에 미치는 영향
임연수,송근호,유명조,서지민,김명철,김덕환,Lim, Yeon-Soo,Song, Kun-Ho,You, Myung-Jo,Seo, Jee-Min,Kim, Myung-Chul,Kim, Duck-Hwan 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.1
The present study was performed to elucidate the effect of canine electroacupuncture anesthesia on vital signs and blood gas values. Groups were divided into experimental (electroacupuncture: EA) and control (ketamine) groups. The vital signs (body temperature, respiration rate and pulse) and blood gas values (pH, $pCO_2$ and $pO_2$) of venous and arterial blood were determined. Body temperatures of EA group were significant higher than than of ketamine group at 15 min., 30 min., 45min. and 60 min. (p<0.05) after anesthesia, respectively. The respiration rates of EA group were higher than those of ketamine group, however, significant differences were not observed between both groups. The pulses of EA group were significant higher than those of ketamine group at 5 min. (p<0.05), 10 min. (p<0.01), 15 min. and 30 min. (p<0.05) after anesthesia, respectively. The arterial and venous blood pHs of ketamine group were slightly higher than those of EA group, respectively, however, no significant differences were found between both groups. Significant differences were not observed between both groups in the arterial and venous blood $pCO_2$, respectively. The arterial blood $pO_2$ of EA group was significant higher than those of ketamine group at 5 min. (p<0.05) after anesthesia. No significant differences were observed between both groups in the venous blood $pO_2$. These results suggest that the changes of vital signs and blood gas values of EA group are similar to those of ketamine group with the exception of changes in the body temperature, pulse and arterial blood $pO_2$.
이스라엘 잉어( Cyprinus carpio)에서 Aeromonas veronii 감염증의 증례 phylogenetic analysis와 항생제 내성
이승원 ( Seung Won Yi ),유명조 ( Myung Jo You ),이해범 ( Hae Beom Lee ),신기욱 ( Gee Wook Shin ) 한국동물위생학회 2012 韓國家畜衛生學會誌 Vol.35 No.3
We reported an outbreak of Aeromonas (A.) veronii responsible for ulcerative dermatitis in Israeli carp (Cyprinus carpio). The major clinical signs were darkening body, abdominal extension, exophthalmos and severe ulcerative necrosis in the skin. The necropsy showed yellowish ascites, necrosis in liver and enlargement of kidney and spleen in the morbid fish. In blood agar for culturing bacteria, three different colonies were identified as A. veronii, Plesimonas shigelloides and Shewanella putrefaciens by phylogenetic identification using 16S rRNA or gyrB gene sequences. A. veronii was the most dominant species among them and was resistant to ampicillin, nalidixic acid and oxytetracycline.
야생너구리(Nyctereutes procyonoides)의 혈액 및 혈액화학치
송근호,유명조,김덕환 충남대학교 수의과대학 동물의과학연구소 2002 動物醫科學硏究誌 Vol.10 No.-
This study was performed to determine the blood and blood chemical values on captured 16 raccoon dogs (Nyctereutes procyonoides). Hematological examination revealed that RBC, Hb, PCV, MCV, MCH and MCHC values were 7.9±0.8x10^(6)/㎕, 14.0±2 g/dl, 44.1±5.5 %, 56.9±3.8 fl, 18.0±1.4 pg and 31.9±2.9 g/dl. respectively, and also revealed that WBC, neutrophil, lymphocyte. eosinophil and monocyte values were 13.6±2.4x10^(3)/㎕, 8.9±2.1x10^(3)/㎕, 2.7±0.8x10^(3)/㎕, 1.1±0.5x10^(3)/㎕ and 1.0±0.4x10^(3)/㎕, respectively. Blood chemical examination showed that total protein, albumin, globulin and A/G ratio values were 7.9±0.9 g/dl, 3.0±0.3 g/dl, 4.8±0.7 g/dl and 0.6±0.1, respectively, and also showed that ALT, AST, BUN. Creatinine and glucose values were 95.8±57.8 1U/ℓ, 64.4±30.1 1U/ℓ, 20.8±5.4 ㎎/dl, 0.7±0.1 ㎎/dl and 58.5±19.1 ㎎/dl, respectively.