재생불량성 빈혈의 병태생리에서 Fas 항원과 Apoptosis의 역할

원종호,이남수,김숙자,정희정,이규택,박성규,백승호,김성일,홍대식,박희숙,Won, Jong-Ho,Lee, Nam-Su,Kim, Sook-Ja,Cheong, Hee-Jeong,Lee, Kyu-Taeg,Park, Seung-Kyu,Baick, Seung-Ho,Kim, Sung-Il,Hong, Dae-Sik,Park, Hee-Sook 대한면역학회 2002 Immune Network Vol.2 No.1

Background: Clinical observations and laboratory studies have supported an immune basis for most acquired aplastic anemias, with the majority of patients responding to immunosuppressive therapy. Fas, a member of the tumor necrosis factor (TNF) receptor superfamily is a critical downregulator of cellular immune responses. Proinflammatory cytokines like interferon gamma (IFN-${\gamma}$) and TNF-${\alpha}$ can induce Fas expression and render hematopoietic progenitor cells susceptible to Fas-induced growth suppression and apoptosis. Methods: In order to investigate the involvement of apoptosis in the pathogenesis of aplastic anemia (AA), we measured the expression of Fas antigen and caspase-3 on bone marrow (BM) mononuclear cells (MNCs) of AA in the presence or absence of IFN-${\gamma}$, TNF-${\alpha}$, or macrophage inflammatory protein 1-${\alpha}$ (MIP-$1{\alpha}$). Results: We confirmed that AA BM MNCs were more apoptotic and highly expressed Fas antigen than normal donors. Stimulation by IFN-${\gamma}$, TNF-${\alpha}$, or MIP-$1{\alpha}$ increased Fas antigen and caspase-3 expression in AA BM MNCs than BM MNCs of normal donors. Anti-Fas monoclonal antibody enhanced IFN-${\gamma}$, TNF-${\alpha}$, or MIP$1{\alpha}$ mediated caspase-3 expression in BM MNCs of normal donors. Among these three cytokines, IFN-${\gamma}$ enhanced apoptosis most strongly via Fas-caspase-3 pathway. Conclusion: These results suggest that Fas signal pathway may play a role in the pathophysiology of aplastic anemia and negative hematopoietic regulators like IFN-${\gamma}$ can induce apoptosis of bone marrow progenitors in part by Fas induction.

동해남부연안 해양환경특성 시공간적 변화

원종호,이용우,Won, Jong-Ho,Lee, Young-Woo 한국해양학회 2015 바다 Vol.20 No.1

동해남부연안에서 해수 중 해양환경특성의 시공간적 분포양상을 살펴보기 위해서 2012년 5월부터 2013년 2월까지 계절별로 현장조사를 실시하였다. 표층수 중 영양염류(용존무기질소, 용존무기인, 용존무기규소)의 농도는 봄과 여름에 비해 수직혼합이 활발하게 일어나는 가을과 겨울에 높게 나타났다. 여름에 chlorophyll a 농도가 높게 나타나 이 시기에 낮은 영양염 농도는 밀도약층의 강화로 인한 아표층으로부터 영양염류의 공급 감소와 식물플랑크톤의 광합성에 의한 소비로 판단된다. 반면 봄에는 chlorophyll a 농도가 낮게 나타나 봄에 표층수 중 낮은 영양염 농도는 육상 및 아표층으로부터 영양염류의 공급 감소에 의한 것으로 판단된다. 조사기간 동안 표층수와 저층수 중 용존무기질소와 용존무기인의 비는 각각 연평균 15.6, 14.8로 유사하였으나, 표준편차는 각각 13.6, 4.2로 표층수가 저층수에 비해 상대적으로 크게 나타났다. 특히, 봄에 용존무기질소에 대한 용존무기인의 비가 상대적으로 낮게 나타나(평균 $8.35{\pm}4.67$) 이 시기에 용존무기질소가 식물플랑크톤의 성장에 제한인자로써 작용했을 것으로 판단된다. In order to elucidate the spatiotemporal variations of marine environmental parameters, we collected seawater samples in the south-western region of the East Sea in May, August, and November 2012 and February 2013. The concentrations of dissolved inorganic nutrients (dissolved inorganic nitrogen, phosphorus, and silicate) in surface seawater during the summer season were lower than those during autumn and winter seasons, which the mixed layer is deeper. The low nutrient concentration in spring and summer seasons seems by consumption of dissolved inorganic nutrients by phytoplankon photosynthesis (high chlorophyll a concentration) and the limited supply of dissolved inorganic nutrients from subsurface layer having high nutrients. The low nutrient concentration during spring season seems to be related to the limited supply of dissolved inorganic nutrients from land and subsurface layer because the concentration of chlorophyll a was low. The DIN:DIP ratio was a wide range of average $15.6{\pm}13.6$ in the surface seawater compared to that of average $14.8{\pm}4.2$ in the bottom seawater during sampling periods. The dissolved inorganic nitrogen might act as a limiting factor of the growth of phytoplankton because the DIN:DIP ratio (on average $8.35{\pm}4.67$) was low during the spring season.

적외선용 광학소자의 초정밀 절삭특성

원종호,박원규,김주환,김건희,Won, Jong-Ho,Park, Won-Kyoo,Kim, Ju-Hwan,Kim, Geon-Hee 한국기계가공학회 2003 한국기계가공학회지 Vol.2 No.1

Machining technique for optical crystals with single point diamond turning tool is reported in tills paper. The main factors influencing the machined surface quality are discovered and regularities of machining process are drawn. Optical crystals have found more and more important applications in the field of modern optics. Optical crystals are mostly brittle materials of poor machinability The traditional machining method is polishing which has many shortcomings such as low production efficiency, poor ability to be automatically controlled and edge effect of the workpiece. SPDT has been widely used in manufacturing optical reflectors of non-ferrous metals such as aluminum and copper which are easy to be machined for their proper ductility. But optical crystals being discussed here are characterized by their high brittleness which makes it difficult to obtain high quality optical surfaces on them. The purpose of our research is to find the optimum machining conditions for ductile cutting of optical crystals and apply the SPDT technique to the manufacturing of ultra precision optical components of brittle materials. As a result, the cutting force is steady, the cutting force range is 0.05-0.08N. The surface roughness is good when spindle is above 1400rpm, and feed rate is small. The influence of depth of cut is very small.

초정밀가공기를 이용한 SIL 렌즈의 절삭특성

원종호,박원규,김주환,김건희,Won, Jong-Ho,Park, Won-Kyoo,Kim, Ju-Hwan,Kim, Geon-Hee 한국기계가공학회 2003 한국기계가공학회지 Vol.2 No.1

The aspherical lenses are used as objective lens of optical pickup. To examine the design factor the sample product is made before manufacturing of injection mould of lens. The optimum cutting condition of PMMA lens sample with ultra precision SPDT, the roam spindle speed, the depth of cut, the feedrate are found. The demanded surface roughness 100m Ra, aspherical form error $0.5{\mu}m$ P-V for aspherical lens of optical data storage device are satisfied.

무산소동 표면의 나노미터단위 초정밀가공

원종호(Jong-ho Won),박상진(Sang-jin Park),오래택(Rae-Taek Oh),김건희(Geon-hee Kim),홍권희(Kweon-hee Hong),도철진(Cheol-jin Do),유병주(Byeong-ju Rui) 한국정밀공학회 1999 한국정밀공학회 학술발표대회 논문집 Vol.1999 No.11월

3차원형상 단면의 원심배럴가공에 관한 연구(1)

원종호(Jong-Ho won),오래택(Rae-Taek Oh),김주환(Ju-Hwan Kim),김우강(Woo-Kang Kim) 한국정밀공학회 1998 한국정밀공학회 학술발표대회 논문집 Vol.1998 No.11월

골수이형성증에서 장기골수배양과 In Situ Nick End Labeling 법을 이용한 조혈장애와 아포토시스에 관한 연구

원종호(Jong Ho Won),홍대식(Dae Sik Hong),박희숙(Hee Sook Park),이정신(Jung Shin Lee) 대한내과학회 1997 대한내과학회지 Vol.53 No.4

N/A Objectives: The myelodysplastic syndromes (MDS) are a group of acquired clonal hematopoietic disorders characterized by peripheral cytopenias and a hypercellular or normocellular dysplastic bone marrow. The mechanisms responsible for development of MDS are not known. We performed this study to evaluate the hematopoietic abnormalities and apoptosis in MDS. Methods: Long-term bone marrow culture (LTBMC) was performed for colony assays, cobblestone area assay, stromal morphologic changes from 7 patients with MDS and 7 normal controls. In situ nick end labeling (ISNEL) method was performed for detection of apoptosis from LTBMC in 7 patients with MDS and 7 normal controls. ISNEL method also performed in bone marrow cell bloc samples in 36 patients with MI3S. Results: Viability of nonadherent cells from LTBMC of patients with MDS was not decreased compared with normal controls at 1 week, but significantly decreased at 2 and 3 weeks compared with normal controls (P<0.0001). Formation of the cobblestone areas from patients with MDS was slightly decreased compared with normal controls at 1st week, but significantly decreased at 2nd and 3rd weeks compared with normal controls (P<0.0001). Slightly decreased compared with normal controls at 1 week, but significantly decreased at 2 and 3 weeks compared with normal controls (P<0.0001). Stromal layers produced in LTBMC of normal controls and 1 patient with MDS were detected at 1 week and were formed confluent stroma from 3 weeks, but another patients with MDS who did not form a confluent stroma. Patients with MDS had significantly lower colony forming unit granulocyte-macrophage (CFU-GM) compared with normal controls at 1 (P<0.01) and 2 weeks (P<0.001) of LTBMC. Two weeks of LTBMC resulted more profound inhibition of CFU-GM formation than 1 week (P<0.0001). Apoptotic cell death was absent in adherent and non adherent cells from normal controls at 1 and 2 weeks, but massive apoptotic cell death was found in adherent and non adherent cells from patients with MDS at 1 and 2 weeks and the degree of apoptosis was profound at 2 weeks compared with 1 week. Among the 36 patients, fifteen patients demonstrated varying degrees of apoptosis positive cells, 4 having low, 8 intermediates, and 3 high scores. Remaining 21 patients showed absent apoptosis or only occasional positive cells. Conclusion: Hematopoietic abnormalities such as a failure of differentiation are caused by the stromal defects and the biologic basis of the apparent paradox of peripheral cytopenias in the face of hypercellular (or normocellular) marrow is related by intramedullary apoptotic cell death of the stromal and hematopoietic cells.

카슨 방정식의 근사모델을 활용한 3상 선로 임피던스의 계산

원종호(Jong-ho Won),이지원(Ji-won Lee),김법수(Beopsoo Kim),김인수(Insu Kim) 대한전기학회 2021 대한전기학회 학술대회 논문집 Vol.2021 No.7

철근이 부식된 철근콘크리트 벽체의 구조성능 평가에 대한 실험적 연구

원종호 ( Won¸ Jong-ho ),한남희 ( Han¸ Nam-hee ),정란 ( Chung¸ Lan ) 한국구조물진단유지관리공학회 2000 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.4 No.2

This paper presents an experimental study on evaluation of structural performance on corroded reinforced concrete walls under only axial load. This test was carried on the thirty-nine reinforced concrete walls with 120×300×500mm size subjected to combined axial load. Effects of key variables such as the corrosion level, the number of horizontal bar, the corrosion rate are studied in this program. The results of this study show that the capacity of wall axial force was decreased as corrosion level was increased, especially, rapid development was shown after 9% corrosion level. Also it was found that influence of corrosion was decreased to number of horizontal bar was increased.

진공 예냉장치 내에서의 야채류의 온도 변화 특성에 관한 연구

원종호(Jong-Ho Won),박상균(Sang-Gym Park),윤석훈(Seoc-Hoon Yoon),오철(Cheol Oh) 한국항해항만학회 2007 한국항해항만학회지 Vol.31 No.10

진공예냉장치에서 상대습도변화 및 냉각온도변화에 따른 야채류의 온도변화에 대하여 고찰한 논문이다. 실험장치는 진공챔버, 이젝트펌프, 냉동기장치, 계측장치 및 냉각수 계통으로 구성되어 있다. 실험결과 야채류의 냉각속도는 야채류의 함수율에 크게 의존하고 있으며, 함수율이 높은 야채가 낮은 야채에 비하여 냉각속도가 증가하는 것을 알 수 있다. This study is to observe the change of temperature and relative humidity for various vegetables in vacuum precooling system. The materials for experiments were the lettuce, chinese cabbage, pak choi and cabbage. The experimental apparatus was constructed of vacuum chamber, vapor/water separator, water tank, pumps ejecting and cooling water circulation, refrigerator unit, cooling coil for water cooling, Hygrometer and Data logger measuring of the temperature change. The experiments were operated in 20torr and recorded every 3 minutes. It was found that the cooling temperature and speed of vegetables are depending on the percentage of its water content. The more water contains, the faster cooling speed and the lower cooling temperature.