형질전환 벼 이용 품종 조기개발 체계 확립

원용재,이기환,조준현,고종민,박향미,한창덕,양세준,김순철,남민희,Won, Yong-Jae,Yi, Gi-Hwan,Cho, Jun-Hyeon,Ko, Jong-Min,Park, Hyang-Mi,Han, Chang-Deok,Yang, Sae-Jun,Kim, Soon-Chul,Nam, Min-Hee 한국식물생명공학회 2004 식물생명공학회지 Vol.31 No.1

This study was carried out to establish a new breeding scheme which is connected with conventional breeding method and anther culture method. To develop a herbicide resistant and direct seeding rice, $F_1$ plants were subjected to anther culture and regenerated plants from 5 crosses were studied to confirm the introduction of bar gene. After PCR analysis, we selected 227 plants which were carrying herbicide resistance gene (bar) out of 1,508 regenerated plants from anther culture. Among 169 $A_2$ lines carrying herbicide resistant gene from 5 crosses including YR23235 (Dongjin Ds3(Ba $r^{R}$)/ Milyang165), 42 lines that had superior agronomic characters were selected for further research. Among them, YR23235Acp79 which showed herbicide resistance, direct seeding adaptability and superiority in major agronomic characters was named Milyang 204. This breeding scheme proved that the anther culture of $F_1$ plants crossed between transformant and cultivar or transform ant alone could be utilized in breeding programs for a rapid progeny fixation and development of a variety.y. 형질전환 식물체를 직접 이용하기에는 후대에 유전적인 고정 및 포장검정이라는 문제가 대두되는데, 이를 해결하기 위하여 본 연구는 바스타저항성 유전자가 도입된 벼와 직파적 응성 계통을 교잡한 후 약배양에 의한 형질전환체 고정 등 기존 육종체계와 연계하여 제초제 저항성 직파적응 계통을 조기에 육성할 수 있는 체계를 확립하고자 수행하였다. 제초제 저항성 직파적응 계통을 조기에 육성하고자 1999년 동계에 교배된 동진벼 Ds15(Ba $r^{R}$)/주남벼 등 5조합으로부터 2000년 $F_1$을 양성하고, 이들을 PCR분석으로 제초제 저항성 유전자 bar도입을 확인한 후 약배양을 실시하여 얻어진 1,508개의 재분화 식물체 중 제초제 저항성 유전자가 도입된 169개체 ( $A_2$)에서 채종하였다. 이들을 2001년 포장에 공시하여 42계통을 선발하고,2002년에 동진벼 Ds15 (Ba $r^{R}$)/주남벼 조합에서 수량 등 주요 농업형질이 우수한 7계통을 생산력 검정시험에 공시한 결과 제초제 저항성이면서 직파적응성 등 기타 농업형질이 우수한 YR23235Acp79를 선발하여 밀양 204호로 계통명을 부여하였다. 결과적으로 형질전환체를 이용하여 교배부터 계통선발까지 3년 만에 목적하는 계통을 조기에 육성할 수 있는 생력육종체계가 확립되었다.

가공용 쌀 품종 및 가공밥 이용기술 개발

원용재(Yong-Jae Won),오세관(Sea-Kwan Oh) 한국식품과학회 2014 식품과학과 산업 Vol.47 No.2

중부지역 적응 조생 복합내병성 고품질 벼 ‘중모1023’

원용재(Yong-Jae Won),조영찬(Young-Chan Cho),최임수(Im-Soo Choi),이점호(Jeom-Ho Lee),이상복(Sang-Bok Lee),이정희(Jeong-Heui Lee),오명규(Myung-Kyu Oh),김정주(Jeong-Ju Kim),양창인(Chang-Ihn Yang),안억근(Eok-Keun Ahn),장재기(Jae-Ki Chang 한국육종학회 2015 한국육종학회지 Vol.47 No.3

The ‘Jungmo1023’ is the japonica rice variety developed from a cross between Yeongdeog34 and F1 of Iksan456 and SR21097-B-B-19-2-2 by the rice breeding team at NICS in 2012. The heading date of ‘Jungmo1023’ is August 3 in middle plain area and it is six days earlier than ‘Hwaseong’. ‘Jungmo1023’ has 80 cm of culm length which is 4 cm shorter than those of ‘Hwaseong’ and 107 spikelet per panicle. The premature heading rate of ‘Jungmo1023’ was 3.3%. It showed resistance to blast, bacterial blight (K1, K2, K3 race) and stripe virus, but susceptibility to K3a race of bacterial blight, dwarf and black streak dwarf viruses and planthoppers. The milled rice of this variety exhibits translucent and medium short grain shape. It has better palatability index of cooked rice (0.24) and lower amylose content (18.0%) than that of ‘Hwaseong’. The characteristics related to grain milling were better than those of ‘Hwaseong’, especially head rice milling recovery ratio and head rice ratio (96.2%). ‘Jungmo1023’ showed 4.56 MT/ha of milled rice productivity at 7 sites in ordinary cultivation. However, ‘Jungmo1023’ showed early cultivation culture adaptability because of 98% of its yield potential (4.99 MT/ha) compared to high yielding early-maturing variety ‘Jopyeong’. ‘Jungmo1023’ could be adaptable to the middle plain, mid-mountainous and north-east coastal area in Korea (Registration No. 5115).

복합내병성 가공밥 적성 벼 ‘미소미’의 농업적 특성

원용재(Yong-Jae Won),양창인(Chang-Ihn Yang),조영찬(Young-Chan Cho),최임수(Im-Soo Choi),김명기(Myeong-Ki Kim),정응기(Eung-Gi Jeong),이점호(Jeom-Ho Lee),이상복(Sang-Bok Lee),백만기(Man-Kee Baek),홍하철(Ha-Cheol Hong),안억근(Eok-Keun Ah 한국육종학회 2016 한국육종학회지 Vol.48 No.3

The Misomi is a rice variety derived from a cross between ‘Sobi’ which has adaptability of low fertilizer cultivation and SR21110 (Ilpum/Jinbu19) by the rice breeding team at National Institute of Crop Science, RDA in 2013. The heading date of ‘Misomi’ is August 14 and six days later than check variety, ‘Hwaseong’. It has 83 cm of culm length and 121 spikelets per panicle. It showed weaker cold tolerance than that of ‘Hwaseong’ during exposed to cold stress. ‘Misomi’ showed resistance to blast disease, K1, K2 and K3 race of bacterial blight and stripe virus, but susceptibility to K3a race, other viruses and planthoppers. The milled rice of this variety exhibits translucent, clear non-glutinous endosperm and short grain shape. It has low protein content (6.2%) and similar amylose content (19.4%) to that of ‘Hwaseong’. ‘Misomi’ showed better palatability index of cooked rice than that of ‘Hwaseong’. Particulary, ‘Misomi’ showed excellent processing adaptability than ‘Chucheong’ and ‘Hwaseong’ on palatability index of cold and aseptic cooked rice. Its milled rice recovery rate is similar to those of ‘Hwaseong’. However, whole grain rate of milled rice is higher than that of ‘Hwaseong’. Misomi has 5.68 MT/ha of milled rice productivity in ordinary cultivation, which was 12% higher than that of ‘Hwaseong’. Misomi could be adaptable to the middle plain area and Midwest coast area in Korea (Grant No. 5635).

강원 영서지역에서 겨울 및 여름 사료작물 이모작 시 생산성

손범영,원용재,김성국,김민태,Son, Beom Young,Won, Yong Jae,Kim, Sung Kook,Kim, Min Tae 한국초지조사료학회 2021 한국초지조사료학회지 Vol.41 No.2

본 시험은 강원 영서지역에서 겨울사료작물(IRG 및 트리티케일)과 여름사료작물(사료용 벼) 이모작 시 양질 조사료의 최대 수량 생산 작목조합을 알아보기 위해 수행하였다. 겨울사료작물의 건물률은 IRG '코윈어리'가 평균 29.6%로 트리티케일 '조성'의 평균 35.5%보다 낮았다. 평균 생체수량은 '코윈어리'가 2,662kg/10a, '조성'이 2,836kg/10a로 두 작목 간 통계적으로 유의적인 차이가 없었다. 평균 건물수량은 트리티케일 '조성(996kg/10a)'이 IRG '코윈어리'(696kg/10a)보다 많았다. 여름사료작물인 사료용 벼의 건물률은 '조농'이 평균 34.5%, '조우'가 평균 35.0%로 유의적인 차이가 없었다. 평균 생체수량은 '조우'가 5,367kg/10a로 '조농'의 3,966kg/10a보다 많았다. 또한 평균 건물수량도 '조우(1,936kg/10a)'가 '조농(1,433kg/10a)'보다 많았다. 겨울사료작물인 트리티케일 '조성'을 10월 중순에 파종하여 이듬해 5월 하순에 수확한 후 여름사료작물인 사료용 벼 '조우'를 6월 상순에 이앙하는 조합으로 2,982kg/10a의 최대 건물 생산량을 얻을 수 있었다. In order to establish optimal double cropping system for getting the maximum annual productivity, we investigated annual fresh and dry yields of winter forage crops (WFC), Italian ryegrass (IRG) 'Kowinearly', triticale 'Joseong' and summer forage crop (SFC), 'Jonong' and 'Jowoo' as whole crop silage rice in the paddy field of Yeongseo of Gangwon Province. The double cropping of each crops was applied with 2 standard cultivation method of WFC and SFC from 2018 to 2020. For the WFC, the average percentage of dry matter (29.6%) of IRG 'Kowinearly' was lower than that (35.5%) of triticale 'Joseong'. The average fresh matter yield of IR G 'Kowinearly' was 2,662kg/10a that there was no significant difference from the 2,836kg/10a of triticale 'Joseong'. The average dry matter yield (996kg/10a) of triticale 'Joseong' was more than that (696kg/10a) of IRG 'Kowinearly'. For the summer forage crops, the average percentage of dry matter of whole crop silage rice, 'Jonong' was 34.5% that there was no significant difference from the 35.0% of 'Jowoo'. The average fresh matter yield (5,367kg/10a) of 'Jowoo' was more than that (3,966kg/10a) of 'Jonong'. And the average dry matter yield (1,936kg/10a) of 'Jowoo' was more than that (1,433kg/10a) of 'Jonong'. The total maximum dry matter yield was 2,982kg/10a with the combination of the WFC triticale, 'Joseong' and the SFC whole crop silage rice, 'Jowoo'. In conclusion, the combination of crops that can obtain the maximum yield of high quality forage for double cropping is sowing the WFC triticale, 'Joseong' in mid-October, harvesting 'Joseong' around the end of May and then SFC whole crop silage rice, 'Jowoo', to be transplanted in early June.

벼 약배양에 효과적인 배지조성 및 저온처리 방법

이기환,원용재,고종민,박향미,조준현,오병근,양세준,김순철,남민희,Yi, Gi-Hwan,Won, Yong-Jae,Ko, Jong-Min,Park, Hyang-Mi,Cho, Jun-Hyeon,Oh, Byeong-Geun,Yang, Sae-Jun,Kim, Soon-Chul,Nam, Min-Hee 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.4

In spite of potential benefits of anther culture, low productivity of plant regeneration in some genotypes; e.g. tonsil and indica rice, is one of the major obstacles for practical use of anther culture. This study was conducted to improve cold shock method and carbohydrate source for increasing the efficiency of anther culture in rice. The most common carbon source, sucrose was replaced to maltose, which has two molecules of glucose. Maltose increased callus induction 1.4-to 1.8-fold higher in japonica rice, 3.2-to 11.6-fold in tongil types and 2.7-fold in indica rice IR50. Callus induction was increased from 0.2% to 12.5% in maltose medium compared to the medium supplemented with sucrose plus glucose in indica rice "Tetep". A simple procedure of vacuum packaging of panicles during cold shock treatment prolonged not only anther viability more than 15 days but also increased callus induction more than 2-fold compared to open-air storage (conventional method). Combining of above two methods, callus induction was increased 28 to 56% in japonica, 13 to 33% in tonsil type and 12 to 31% in indica rice. Plant regeneration was increased 14 to 35% in japonica, 10 to 20% in tonsil and 4 to 15% in indica rice, respectively.

조생종 벼의 평야지 재배에 따른 등숙 온도 차이에 의한 전분구조 및 종자 저장단백질 특성 연구

곽지은,이점식,원용재,박향미,곽강수,김미정,이춘기,김선림,윤미라,Kwak, Jieun,Lee, Jeom-Sig,Won, Yong-Jae,Park, Hyang-Mee,Kwak, Kang-Su,Kim, Mi-Jung,Lee, Choon-Ki,Kim, Sun-Lim,Yoon, Mi-Ra 한국작물학회 2018 한국작물학회지 Vol.63 No.2

This study was performed to understand the effects of filling stage temperature on the characteristics of starch and storage protein and the quality of rice grains. Eight early maturing rice varieties were cultivated in Cheolweon (latitude $38^{\circ}15^{\prime}N$) and Suwon (latitude $37^{\circ}16^{\prime}N$) areas in Korea. Rice grown in Suwon, with relatively high ripening period temperatures, showed significantly reduced head rice ratio and eating qualities, higher protein and lower amylose contents than rice grown in Cheolweon. In rice that ripened under high temperature conditions, the starch contained significantly less short-chain amylopectin (DP < 12) but more intermediate- (DP 13-24) and long- (DP > 25) chain amlylopectin compared to rice that ripened under normal conditions. In addition, the electrophoretic pattern of rice storage protein under high- temperature conditions revealed decreased prolamin and increased glutelin contents.

호주의 쌀 산업 현황과 전망

정응기 ( Eung-gi Jeong ),원용재 ( Yong-jae Won ),이정희 ( Jeong-heui Lee ),안억근 ( Eok-keun Ahn ),김민태 ( Min-tae Kim ),윤순덕 ( Soon-duck Yoon ),하운구 ( Woon-goo Ha ),이점호 ( Jeom-ho Lee ) 한국국제농업개발학회 2015 韓國國際農業開發學會誌 Vol.27 No.5

1. 호주는 최근 몇 년간 반복된 가뭄과 홍수로 강수량이 불규칙해지면서 쌀 생산에 차질이 있었으며, 평년의 강수량을 기록할 경우 호주의 쌀 생산량은 연간 80 ~ 100만톤 정도 될 것으로 추정된다. 2. 호주의 한국 쌀 수출은 교민시장 위주로 접근하고 있으나, 현지시장에 진입하려면 가격격차를 줄이는 것이 가장 시급하다고 판단되며, 일본에서 수출되는 쌀과 비슷한 수준으로 미국이나 호주 쌀에 비해서 우수한 것으로 인정되고 있다. 3. 최근 호주는 일식이 대중화되는 추세에 부응하여 초밥용쌀 시장을 집중적으로 공략할 필요성이 있다고 판단된다. 4. 호주에서 쌀을 비롯한 우리나라 농산물의 수출이 증가하고 있으나 그를 취급하는 유통업자가 소규모로 분산되어 있어규모화가 필요하다고 판단된다. 5. 한국 쌀 수출증대를 위한 개선방안으로 KOTRA, 농수산식품유통공사 등 현지사무소를 확장하고 우리나라 음식문화와 연계한 마케팅 활동 강화가 요구된다. Australia records approximately 80 ~ 100 tons annual production. However, in recent years, Australian rice production has fallen down due to irregular and repeated droughts and floods. The Republic of Korea exports rice to Australia and the major consumers are Korean immigrants. To enter the local market, it seemed that the most urgent objective to achieve is to reduce the price gap with the imported Japanese rice, which has been recognized as the superior rice compared to local rice and those from United States. Recently, it determined that Australia is in the growing needs of marketing rice for Japanese sushi. The response to this popular trends: The scaling up the size of distributors handling the Korean agricultural products in Australia is required. The expanding networks among KOTRA and aT center as well as the local offices will increase rice exports from Korea. Marketing activities can be leveraged by the harmonized plan for spreading out the Korean food cultural system.

이모작 적응 조생 복합내병충성 고 바이오매스 사료용 벼 ‘조우’

안억근(Eok-Keun Ahn),원용재(Yong-Jae Won),이점호(Jeom-Ho Lee),정응기(Eung-Gi Jeong),박향미(Hyang-Mi Park),홍하철(Ha-Cheol Hong),장재기(Jae-Ki Chang),현웅조(Ung-Jo Hyun),정국현(Kuk-Hyun Jung),이정희(Jeong-Heui Lee) 한국육종학회 2021 한국육종학회지 Vol.53 No.2

‘Jowoo’, an early maturing, high biomass yielding rice (Oryza sativa L.) cultivar with high feed value and multiple disease-insectresistance, was developed for winter forage crop-whole crop silage (WCS) rice double cropping. It was derived from a cross between a highbiomass and multiple disease resistant line ‘Suweon519’ and an early flowering, high yielding Tong-il type variety ‘Gaya’ that harbored Bph3and BPH26, conferring brown planthopper (Bph) broad resistance. This cultivar had a growth period of approximately 106 days from seedingto heading, 97 cm culm length, 24 cm panicle length, 12 panicles per hill, 120 spikelets per panicle, and 1,000-grain weight of 22.1 g asbrown rice in the central plain region of Suwon. This early flowering rice variety was tolerant to premature germination and cold stresses,especially showed higher grain fertility than ‘Nokyang’ at a cold-water (17℃) irrigated screening nursery. Further, ‘Jowoo’ exhibited high lodgingresistance in the yellowing ripening stage, an optimal period for harvest. In addition, ‘Jowoo’ was resistant to leaf and neck blast, bacterialblight (race K1, K2, K3, K3a), rice stripe virus, and small Bph and was also moderately resistant to Bph. Its average dry matter yield forthree years reached 18.2 MT/ha, the highest among early flowering rice varieties and 19% higher than that of ‘Nokyang’. This cultivar had72.2% total digestible nutrients, which is also higher than that of ‘Nokyang’. In the Korean peninsula, ‘Jowoo’ grows well in the centraland southern plains and can be harvested 30 days after heading. This helps improve its feeding value and digestion rate in livestock (GrantNo. 8133).

벼 分蘖開度의 遺傳

고희종(Hee Jong Koh),원용재(Yong Jae Won),차건완(Kean Wan Cha),이종철(Jong Cheol Lee),박순직(Sun Zik Park),허문회(Mun Hue Heu) 한국육종학회 1997 한국육종학회지 Vol.29 No.4

This study was carried out to investigate the inheritance mode of tillering angle using wx126-based near isogenic lines of different tillering angle, Broom, Open and Spread type, and the tillering angle of 73 rice varieties from Korea and IRRI was also estimated. The average tillering angle of rice varieties from Korea and IRRI were 10.5˚ (wide side) and 6.7˚ (narrow side) in japonicas, 12.3˚ (wide side) and 8.3˚ (narrow side) in Tongil type, and 8.7˚ (wide side) and 5.9˚ (narrow side) in Indicas. Average tillering angle at wide side of wx126 (original variety), Broom, Open, and Spread type at heading stage were 8.1˚, 5.5˚, 14.8˚, and 33.9˚, respectively, and the difference of tillering angle among the lines was maintained throughout the whole growth stage. In six F₂ populations of all-possible crosses among three near-isogenic lines, tillering angle showed continuous variation skewed to narrower parent, implying that it should be a quantitative character and the genes conferring the narrower tillering angle might be dominant over those conferring the wider tillering angle. Broad sense heritabilities for tillering angle varied 48.8% to 76.2%.