투과성 Filter 에 배양한 Opossum Kidney ( OK ) 세포에서 유기물질의 이동 특성

정미혜(Mi Hye Jung),우재석(Jae Seok Woo),이상호(Sang Ho Lee) 대한내과학회 1996 대한내과학회지 Vol.50 No.5

N/A Objectives: Opossum kidney cells were cultured as monolayer on permeable filters and the characteristics regarding the transepithelial resistance and polarized uptake or transepithelial transport of organic compounds were determined. So, we want to know the usefulness of OK cells as a experimental model for the study of proximal tubular transport. Methods: OK cells were grown as monolayer on permeable filters of Transwell chamber. The seeding cell density was 10(3) cell/Cm2 and OK cells were incubated in Dulbecco`s Modified Eagles medium. The used organic compounds were [14Cl labelling mannitol, β-methylglucose, cycloleucine, TEA, We measured age dependent change of cell density, transepithelial resistance, polarized uptake or transepithelial transport of organic compounds. Results: transepithelial resistance increased, whereas transepithelial mannitol flux decreased, with aging and reached the maximum level 6days after seeding, which indicate formation of functional tight junction. On polarized uptake studies for α-methylglucose, an nonmetabloized analogue of glucose, OK cell monolayer expressed a Na+-dependent, phlorizininhibitable uptake in their apical membrane and a Na+-independent, phloretin-inhibitable uptake in their basolateral membrane. Uptake studies for cycloleucine, an unmetabolized neutral amino acid analogue, from either side of monolayers indicated that OK cells accumulated cycloleucine through both sides of membranes via both Na+-dependent and -independent mechanisms but with a different Na+-dependency (44% for apical and 29% for basolateral membrane). Transepithelial transport studies for tetraethylammonium, a typical organic cation, showed a unidirectional transcellular transport from basolateral to apical side which was stimulated by acidic pH of apical medium. Conclusion . These results suggest that OK cell monolayers grown on permeable filters developed functional tight junction and membrane polarity and that OK cells might be a good experimental model for the study of proximal tubular transport.

정보기술을 활용한 의학교육시스템의 설계와 개발: 부산대학교 의학전문대학원 사례

임선주,이상엽,백선용,우재석,감비성,Im, Sun Ju,Lee, Sang Yeoup,Baek, Sun Yong,Woo, Jae Seok,Kam, Beesung 연세대학교 의과대학 2014 의학교육논단 Vol.16 No.1

The development of information technology (IT) has led to changes in medical education. IT has been used for e-learning and e-teaching, e-assessment, e-logistics, and e-administration. Pusan National University School of Medicine has developed its own educational information system using IT to support students' learning and assessment and to manage curricular activities. Based on our experience, we propose six suggestions for designing new software, specifically regarding simplifying the design for users, communication with the programmer, a rapid cycle from design to implementation, orientation support for users, backup and security support, and obtaining patents. Collaborating with the Department of Medical Informatics within the School of Medicine has advantages, and an alliance among medical schools can simplify the development of software. In any case, curricular innovation should precede the introduction of technology because all technologies should support curricular goals.

가정의학 임상실습과정에 대한 구조화된 평가

박은주,이상엽,임선주,윤소정,감비성,백선용,김윤진,우재석,이정규,정동욱,조영혜,이유현,탁영진,Park, Eun Ju,Lee, Sang Yeoup,Im, Sun Ju,Yune, So Jung,Kam, Beesung,Baek, Sun Yong,Kim, Yun-Jin,Woo, Jae Seok,Lee, Jeong-Gyu,Jeong, Dong-Wook,Cho, Yo 연세대학교 의과대학 2017 의학교육논단 Vol.19 No.1

This study was conducted to analyze the strengths and weaknesses of a 3-week family medicine clerkship program based on the results of an online survey taken by the students (N=127) and a structured interview with a focus group (n=10), aimed to improve the quality of the clerkship program. The online survey contained questions pertaining to goals, schedule, contents, arrangement, atmosphere, environment, evaluation, and satisfaction regarding the clerkship. The focus group interview addressed the schedule and achievements of the program. Scores were reported on a 5-point Likert scale. Most students were highly satisfied with the overall quality of the clerkship. The structured interview results showed that 97.6% of the clerkship program was executed according to the schedule. The focus group reported a perfect score of 5 points on several measures including: accomplishment of the educational goals of the family medicine clerkship, providing many chances to obtain medical histories and perform physical examinations on real patients, experience with various symptoms and diseases, positive attitudes of faculty members when teaching, notification of the guidelines for evaluation beforehand, well-constructed and effective clerkship schedule, and reflection of student feedback. However, the focus group gave low scores on: support for health accidents of students, access to patient information, enough opportunities to practice clinical skills, appropriate rest facilities for students, and fairness of clerkship evaluation process. In conclusion, the structured evaluation performed after the 3-week clerkship program motivated students and helped them ensure an efficient clerkship. This structured evaluation also suggested basic data to make the professor who is subject of the assessment. This study shows that structured assessment is an effective method which can be used to improve the quality of clerkships.

Corrigendum: Correction of Acknowledgments

박은주,이상엽,임선주,윤소정,감비성,백선용,김윤진,우재석,이정규,정동욱,조영혜,이유현,탁영진,Park, Eun Ju,Lee, Sang Yeoup,Im, Sun Ju,Yune, So Jung,Kam, Beesung,Baek, Sun Yong,Kim, Yun-Jin,Woo, Jae Seok,Lee, Jeong-Gyu,Jeong, Dong-Wook,Cho, Yo 연세대학교 의과대학 2017 의학교육논단 Vol.19 No.2

의과대학 학생의 맨눈해부학 지식에 대한 임상교수의 인식도 조사

임선주(Sun-Ju Im),감비성(Bee-Sung Kam),이상엽(Sang-Yeoup Lee),우재석(Jae-Seok Woo),이종태(Jong-Tae Lee),이상화(Sang-Hwa Lee),임학(Hak Im),조경제(Gyeong-Je Cho),백선용(Sun-Yong Baek) 대한체질인류학회 2014 해부·생물인류학 (Anat Biol Anthropol) Vol.27 No.4

의과대학과 의학전문대학원에 통합교육과정이 도입되면서 임상실습 기간의 확대로 기초의학의 강의 시간이 줄어들었으며, 대학에 따라서 맨눈해부학 교육의 시간과 내용은 상당한 차이를 보이고 있다. 이에 따라 재학생과 졸업생의 해부학 지식의 저하에 대한 우려가 제기되고 있다. 해부학 교수와 학습의 방향 설정의 기초 자료로 활용하기 위하여 임상실습 과정 학생들의 맨눈해부학 지식과 교육과정 내에서 해부학 교수법의 현 상태에 대하여 임상실습을 지도하는 임상 교수의 인식도에 대한 조사가 필요하였다. 부산·경남지역의 5개 의과대학에 근무하는 임상교수를 대상으로 학생들의 맨눈해부학 지식에 대한 인식도를 설문 조사하여 통계분석을 하였다. 임상교수의 입장에서 졸업생의 임상해부학 지식 정도(2.4±0.6)와 고학년 학생의 전반적인 해부학 지식(2.6±0.7)의 저하를 우려하였으며, 선택 집중해부실습(3.8±0.9)과 해부학 교육 시기 확대(3.3±1.0)에 긍정적이었다. 학생들의 전반적 국소해부학 지식의 수준에 대해서는 외과계열과 영상의학, 외과계열과 기타계열 사이에 유의한 차이를 보였으며(p?0.05), 외과 계열이 해부학 지식수준의 저하를 가장 우려하였다. 해부학교육 시기의 확대에 대해서는 영상의학이 내과, 외과 및 기타 계열과 유의한 차이를 보여 가장 긍정적인 응답을 하였다(p?0.001). 학생들이 해부학 지식이 낮은 원인으로는 임상맥락이 적은 해부학교육이 가장 높았으며, 그 다음으로 통합교육과정에 따른 해부학의 정체성 감소였다. There is concerns about the perceived decline in the knowledge of gross anatomy of the medical students and postgraduate trainees. It is partly caused by the introduction of integrated medical curriculum and the shortage of basic medical science program and the extension of clinical clerkship consequently. There is widespread variability in the teaching style and anatomical curricular content at the medical school in Korea. Despite these changes in the anatomical education, there have been few attempts to assess the opinions of senior clinical teachers on the state of anatomical knowledge of students and the place of anatomy teaching within the curriculum. We sought the views of the clinical teachers on the adequacy of the anatomical knowledge of current students and recent graduates of 5 medical schools in Pusan and Gyeongsang-Nam do areas. Most of the clinical teachers were of the opinion that current medical students have an insufficient anatomical knowledge. They indicated the causes of decline were the clinical irrelevant anatomical teaching content and weakness of identity of anatomy by the introduction of the integrated medical education program. There was widespread support for both the concepts of spreading anatomy teaching throughout the medical course, and an optional, clinically related final year student project in the anatomy.