Effect of Standardized Boesenbergia pandurata Extract and Its Active Compound Panduratin A on Skin Hydration and Barrier Function in Human Epidermal Keratinocytes

우선욱,임동빈,김창희,황재관 한국식품영양과학회 2015 Preventive Nutrition and Food Science Vol.20 No.1

The skin plays a key role in protecting the body from the environment and from water loss. Cornified envelope (CE) and natural moisturizing factor (NMF) are considered as the primary regulators of skin hydration and barrier function. The CE prevents loss of water from the body and is formed by cross-linking of several proteins. Among these proteins, filaggrin is an important protein because NMF is produced by the degradation of filaggrin. Proteases, including matriptase and prostasin, stimulate the generation of filaggrin from profilaggrin and caspase 14 plays a role in the degradation of filaggrin. This study elucidated the effects of an ethanol extract of Boesenbergia pandurata (Roxb.) Schltr., known as fingerroot, and its active compound panduratin A on CE formation and filaggrin processing in HaCaT, human epidermal keratinocytes. B. pandurata extract (BPE) and panduratin A significantly stimulated not only CE formation but also the expression of CE proteins, such as loricrin, involucrin, and transglutaminase, which were associated with PPARa expression. The mRNA and protein levels of filaggrin and filaggrin-related enzymes, such as matriptase, prostasin, and caspase 14 were also up-regulated by BPE and panduratin A treatment. These results suggest that BPE and panduratin A are potential nutraceuticals which can enhance skin hydration and barrier function based on their CE formation and filaggrin processing.

밭 雜草로부터 生理活性物質 探索

金吉雄,白鏡換,禹宣旭 慶北大學校 1987 農業科學技術硏究所報 Vol.4 No.-

바랭이外 48種의 雜草를 對象으로 水溶, 알코올 抽出液 및 標準 페놀 化合物에 對한 상치및 벼의 發芽生長에 미친 影響을 究明하여 얻어진 結果를 要約하면 다음과 같다. 1. 雜草의 水溶抽出液은 1% 濃度에서도 상치 發芽를 크게 抑制시켰는데 쇠별꽃의 抽出液은 65%, 며느리배꼽의 抽出液은 60%, 쥐깨풀의 抽出液은 53.5%의 抑制를 보였고, 水溶抽出液5%및 10% 濃度에서 상치種子의 發芽를 完全抑制시킨 것은 방가지똥, 새완두, 비름, 쇠별꽃, 박주가리, 메꽃, Galinsoga ciliata 等의 雜草였다. 2. 알코올抽出液은 水溶抽出에서 보다 상치의 發芽를 더 抑制시켰다. 1%의 低濃度에서는 벌노랑이 87.5%, 가막사리 77.5%, 며느리배꼽 72.5%, 독말풀 70%, 조뱅이 65%였으며, 5%및 10%의 濃度에서는 메꽃 外 25種의 抽出液이 상치의 發芽를 완전히 抑制시켰다. 3. 標準 페놀 物質인 ferulic과 protocatechuic 酸 等은 상치의 發芽를 크게 抑制시켰으며 벼의 幼苗生育에 크게 影響을 미친 것은 ferulic, umbelliferone, t-cinnamic 酸等이였다. This experiment was conducted to investigate the allelopathic potential of 49weed species including Digitaria sanguinalis. Through the aqueous extracts and the alcohol extracts of them which have been used in the germination of lettuce (Lactucasativa) seeds. Alcohol extracts showed higher inhibitory effect on lettuce seed germination than water extract effect on lettuce seed germination, showing inhibition of 87.5% by Lotus corniculatus, 75% by Bidens trondosa, 72.5% by Polygonum perfoliata, 70% by Datura stamonium and 65% by Cephalonoplos segetum. The increased concentration of alcohol extract from 25 weed species up to 5 and 10% completely inhibition the germination of lettuce seeds. Among 13 standard phenolic compounds ferulic and protocatechuic acids followed by syringic acid at 10???³M significantly inhibited the germination of lettnce seed. Ferulic acid and umbelliferone markedly inhibited the growth of rice shoot and root.

여뀌·마디풀로부터 相互對立抑制作用物質의 分離·同定

禹宣旭(Woo, S. W.),金吉雄(K. U. Kim) 韓國雜草學會 1987 Weed&Turfgrass Science Vol.7 No.2

논·밭 雜草種子의 休眠에 關한 硏究

鄭昶國,禹宣旭,金吉雄,李在鎬 慶北大學校 1986 農業科學技術硏究所報 Vol.3 No.-

논 및 밭 一年生 雜草種子의 休眠性을 檢定하기 위하여 光ㆍ低溫 및 變溫處理가 發芽에 미치는 影響을 究明하여 얻어진 結果를 要約하면 다음과 같다. 갓 採取한 논ㆍ밭 雜草種子는 置床 後 6週까지 光狀態下에서 논 雜草는 바람하늘지기 58.3%, 마디꽃 36.7%, 밭뚝외풀 30.0%, 밭 雜草는 망초 91.7%, 참소리쟁이는 20.0%의 發芽率을 보였다. 반면에 暗狀態下에서는 밭 雜草인 망초의 發芽率이 90.0%, 참소리쟁이 5.0%를 보인 것 이외는 全供試雜草의 種子가 發芽되지 않아 休眠이 있는 것으로 판단된다. 光ㆍ暗狀態下에서 發芽되지 않던 사마귀풀, 돌피, 알방돈사니, 파대가리, 여뀌, 여뀌바늘 等의 논 雜草는 2℃의 低溫 前處理를 함으로써 發芽가 크게 增加되었으며, 밭 雜草는 비름과 참소리쟁이에 低溫 前處理의 效果가 컸다. 變溫處理는 돌피, 바람하늘지기, 고마리, 마디꽃, 여뀌, 여뀌바늘 等의 雜草種子 發芽에 매우 效果的이었다. This experiment was conducted to determine the presence of seed dormancy and the effects of light and temperature on breaking of seed dormancy of 13 paddy and 16 upland weed species. Most of the freshly harvested seeds showed no germination at all regardless of light or dark conditions. Only 5 species such as Fimbristylis miliacea, Rotara indica, Lindernia procumbens, Erigeron canadensis, and Rumex japonicus were germinated under light conditions, showing 58.3%, 36.7%, 30.0%, 91.7% and 20.0%, respectively. pretreatment with low temperature (2C) enhanced germination of most of paddy weed species under light condition, but some of upland weed species were affected by it under light condition, indicating that low temperature treatment is quite effective factor in breaking seed dormancy. One hundred % of germination was observed in Ameileam keisak and Lindernia procumbens, due to the pretreatment with low temperature, and 96.7%, in Rotara indica, 90.0% in Lindernia prostrata 93.0% in Rumex japonicus, 91.7% in Dopatrium junceum and 90.0% in Amaranthus mangostanus, respectively. Altering temperature was somewhat effective to break seed dormancy of paddy weed species, but its effectivity was much less than that of low temperature treatment.

땅콩밭에 發生하는 優占雜草와 競合

崔大雄,禹宣旭,鄭相煥,李相百,金吉雄 慶北大學校 1987 農業科學技術硏究所報 Vol.4 No.-

땅콩밭에 發生하는 主要雜草를 調査하고 雜草發生이 땅콩의 收量에 미치는 影響을 究明하였던 바 얻어진 結果는 다음과 같다. 우리나라 南部地方의 땅콩밭에 發生된 主要雜草는 쇠비름, 속속이풀, 바랭이, 방돈산이, 좀명아주, 피 等인데 이들은 모두 一年生雜草였으며 이들 雜草 中 쇠비름이 가장 優占하는 雜草로 判明되었다. Simpson's index 는 5月30日에 0.480이던 것이 점차 낮아져 8月20日 傾에 0.320, 收穫期인 9月13日에는 0.193으로 낮아지는 傾向을 보여 初期에 特定雜草가 優占發生하다가 後期는 發生草種이 多樣해짐을 나타내고 있다. 땅콩밭에 發生한 雜草의 乾物量은 播種後 90日까지 繼續 增加하여 546.1g/㎡이 되고 時間이 經過함에 따라 減少하였다. 이 時期의 雜草構成比를 보면 廣葉雜草가 60%, 禾本科가 約 40%여서 廣葉雜草가 優占 발생하였다. 땅콩과 雜草와의 競合에 있어서 全 生育期間동안 競合할 境遇 땅콩의 收量이 顯著히 感少하였으며, 땅콩의 收量減少를 막기 위해서는 雜草가 없는 狀態로 유지하든지 또는 땅콩 播種後 30日 以後부터는 雜草를 철저히 防除하여야 한다. This study was conducted to investigate the important weeds occurring in peanut field and to evaluate competitative effect of weeds in peanut yield. In terms of quantity occured, Portulaca oleracea, Rorippa islandica, Digitaria sanguinalis, Cyperus amuricus, Chenopodium ficifolium and Echinochloa crus-galli appeared to be the major weeds in peanut fields. Among those, Portulace oleracea was the most dominant weed species in peanut fields. Based on the morphological classification annual broadleaves become dominant composing 79% of total dry weight at the early stage and in the late stage annual grasses became dominant composing 79% of total dry weight. The relatively higher Simpson's index such as 0.408 in the early stage than those in the late stage of peanut indicates that Portulaca oleracea presented in a single dominant species and afterward weed species become more diversified. It needed to maintain weed free condition for 75-90days after seeding of peanut and weed competition showed be terminated within 30-60days after seeding, in order to obtain peanut yield equivalent to weed free condition.

The Korean Pharmacogenomic Database at NIFDS: 2008 Update

강태선,우선욱,박현주,한순영,박미혜,정면우 한국유전체학회 2009 Genomics & informatics Vol.7 No.3

Since its first release in 2007, the National Institute of Food and Drug Safety Evaluation (NIFDS) has provided pharmacogenomic and comparative information specific to Koreans to allow regulatory reviewers and researchers to adapt their working practices to pharmacogenomics. The highlights of this year’s additions include "Drug Information", "Gene Information" and "Pharmacogenomic information in the drug labels" sections. These new additions provide information on 737 genes, 719 drugs and pharmacogenomic data of the labels or relabels of 253 approved drugs as of November 2008. The latest version of the Korean Pharmacogenomic Database (KPD, release 2.0) has expanded significantly since its previous release. More SNP and haplotype information has been added to the database with the latest version of the KPD containing approximately four times as many SNPs and haplotypes than the previous version (719 vs. 152, and 30 vs. 7 respectively). Through the "SNP" and "Haplotype" sections, the KPD provides unique Korean SNP and haplotype information as well as comparative information of other populations (Japanese, Chinese, European, African) to offer a range of pharmacogenomic data that can help reviewers and the public understand pharmacogenomic information. The quality and quantity of information in the KPD has also been improved considerably. This data can be found at: http://www.nitr.go.kr/nitr/contents/m134700/view.do/.

Lactobacillus curvatus HY7601 and Lactobacillus plantarum KY1032 Cell Extracts Inhibit Adipogenesis in 3T3-L1 and HepG2 Cells

정운희,심재정,우선욱,심재헌,이정렬 한국식품영양과학회 2018 Journal of medicinal food Vol.21 No.9

Some lactic acid bacteria (LAB) and their cellular components have antiobesity effects. In this study, we evaluated the antiadipogenic effects of a mixture of two LAB—Lactobacillus curvatus HY7601 and Lactobacillus plantarum KY1032—using 3T3-L1 preadipocytes and HepG2 hepatocarcinoma cells. 3T3-L1 cells treated with a 1:1 ratio of HY7601 and KY1032 during differentiation showed reduced lipid accumulation by Oil Red O staining, as well as decreased leptin secretion and mRNA expression of peroxisome proliferator-activated receptor-γ and CCAAT/enhancer binding protein-α. HY7601 and KY1032 treatment also suppressed mitochondrial biogenesis and inhibited the expression of genes encoding mitochondrial transcription factors, as well as those related to fatty acid synthesis in HepG2 cells. The antiadipogenic effects of LAB were associated with the cell membrane fraction. These results demonstrate that a mixture of two LAB (HY7601 and KY1032) inhibits adipogenesis in preadipocytes and liver cells and is a potential therapeutic strategy for the treatment of obesity.

Optimization of High Hydrostatic Pressure Process for the Extraction of Kirenol from Siegesbeckia orientalis L. Using Response Surface Methodology

김미보,박지은,우선욱,임상빈,황재관 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.3

High hydrostatic pressure (HHP) extractionmethod was optimized to maximize the extraction yield ofkirenol from Siegesbeckia orientalis. Operating parameterssuch as extraction pressure (100-600 MPa), pressureholding time (3-20 min), feed-to-solvent ratio (1:10-1:90(w/v)), and solvent (ethanol) concentration (0-100%) wereinvestigated individually by mono-factor experiments. Then, the optimal extraction conditions were determinedusing response surface methodology. Box-Behnken designwas applied to evaluate the effects of three independentvariables (extraction pressure, solvent concentration, andfeed-to-solvent ratio) on the extraction yield of kirenolfrom S. orientalis. Extraction pressure, solvent concentration,and feed-to-solvent ratio affected the extraction yieldssignificantly, whereas the pressure holding time had nosignificant effect. The optimal processing conditions,which gave a maximum extraction yield of 85.9% kirenolfrom the raw material, were as follows: extracting pressure320MPa, pressure holding time 5min, ethanol concentration18%, and feed-to-solvent ratio 1:76 (w/v).

마약류 의약품의 품질 확보

박은혜,윤미옥,정주연,송윤경,이지현,엄정윤,남승연,우선욱,김대병,김인규 한국에프디시규제과학회(구 한국에프디시법제학회) 2009 FDC법제연구 Vol.4 No.2