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Selective Extraction and Quantification of Glutathione using Maleimide-Presenting Gold Nanoparticles
오홍석,Jeongwook Lee,여운석 대한화학회 2014 Bulletin of the Korean Chemical Society Vol.35 No.10
In this paper, we describe a new method for the selective extraction and quantification of glutathione (GSH) using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and maleimide-presenting gold nanoparticles (Mal-AuNPs). Our strategy utilizes the Michael addition to selectively extract GSH, from chosen samples, onto the maleimide of Mal-AuNPs. After the extraction step, the GSH bound to the AuNPs was analyzed by MALDI-TOF MS in the presence of an internal standard which was prepared by reacting Mal-AuNPs with isotope-labeled GSH (GSH*). The GSH* has the same structure as GSH but a higher molecular weight, and therefore, enables absolute quantification of GSH by comparing the mass signal intensities of the GSH- and GSH*-conjugated alkanethiols. Our strategy was verified by analyzing GSHspiked fetal bovine serum and NIH 3T3 cells.
오홍석 한국국제지역사회개발학회 2002 地域社會開發學術誌 Vol.12 No.2
Recently, our society is facing multiple crisis in economic and social aspect. This multiple crisis result from not accumulating social capital based on trust in social regulation and morality. Accordingly, in order to solve this problem we make effort to increase social capital. Social Capital is actual proved nonformal norm facilate people relationship. This is essential factor for the purpose of developing in organization. Accordingly, this study investigated the factor forming social capital in organization. The result of multiple literature investigation was that forming factor social capital in organization composed of individual socialeconomic setting in organization member and group participation and activity.
오홍석 한국환경교육학회 1992 環境 敎育 Vol.3 No.1
In the orient, ancestor's perception to natural environment was related on the change of climate. Man had been a essence of the warm blood animal, which had suited in mild climate for living and acting. The weather of hot or cold was not only comfort in human life but influences negatively on human behavior. The most people of oriental was particularly interrelated to seasonal change of climate in earlier time. Because, there are elements such as temperature, humidity and wind in the category of climate, these elements differentiated seasonal change. The main methods of perception of natural environment were observed and classified. Although these methods were in the primitive stage, these coincided the tendency of modern science. For example, confucian was recognized the law of vertical air current that warm air rised and cold air sinked. And they could classify all elements nature based on the principles of 'Yin and Yang(陰陽論)' such as male and female, the sun and moon. shade and light etc. Thus results of the observation and the classification concerned with physical environment can be utilize a wisdom for progressive life of inhabitants. It was a origin of the education in natural environment. Commonness in the viewpoint of environment in the orient if recognized the law of circulation. Buddhism, originated in india, realized that all of phenomena in the world was changed slowly through the principles of cycle(輪廻說) such as male and female, the sun and moon, shade and light etc. Thus results of the observation and the classification concerned with physical environment can be utilized a wisdom for progressive life of inhabitants. It was a origin of the education in natural environment. Commonness in the viewpoint of environment in the orient if recognized the law of circulation. Buddhism, originated in India, realized that all of phenomena in the world was changed slowly through the principles of cycle(輪廻說). For example, whole of land is shifted from young stage to old stage as the life cycle of humanbeing. The theory of karma effects(葉報設) is so signify with the good result that good one's action is reap a sweet fruit. The most environmental problem in today must realized as a consequence of men's act. Then Taoism emphasized the pure nature without the artificiality. Because complexual environment was composed of several elements, It was maintained through the artificiality. Because complexual environment was composed of several elements, It was maintained through the interaction of cause and effects. The solution of environmental problem is maintenanced the harmony between cause and effects by a philosophical concept.