녹내장 동물모델에서 단풍나무 잎 열수 및 에탄올 추출물의 안압 및 신경세포 보호 효과

오태우,박광일,마진열,Oh, Tae Woo,Park, Kwang-Il,Ma, Jin Yeul 대한한의학방제학회 2021 大韓韓醫學方劑學會誌 Vol.29 No.2

Objectives : This study was conducted to evaluate the Intraocular pressure (IOP) and retina neuroprotective effect of Acer palmatum thumb. extract in glaucoma of DBA2/J mice vivo model. Methods : The amount of change in intraocular pressure(IOP) was measured every two weeks for 30 weeks in the DBA2/J glaucoma animal model. The increased IOP were regrouping into drug treatment groups. EW and EE (1, 2 mg/㎖)extract was eye drop administered twice per day for 8 weeks. After administration of the extract, IOP was measured every 1 week at a fixed time for 8 weeks, and protein expression levels and serological changes related to retinal function were investigated. Results : The DBA2/J mice were IOP increased as time dependent, resulting in damage to the retinal function protein related in glaucoma animal model. However, administration of extracts from Leaves of Acer palmatum thumb. prevented an increase IOP, increased for Brn3α, PKCα, and OPN1SW. Additionally, the experssion of Aterocyte maker, GFAP was decreased in DBA2/J mice. Conclusions : These results demonstrate that both IOP and age can be considered in studies using DBA2/J mice and suggest that the extracts from Leaves of Acer palmatum thumb. regulating of IOP and expression of proteins related to retinal function.

갈근이 고지방·고탄수화물식이와 저용량 streptozotocin-유도 당뇨병 마우스에 미치는 효능 연구

오태우 ( Tae Woo Oh ),박용기 ( Yong Ki Park ) 대한본초학회 2014 大韓本草學會誌 Vol.29 No.5

Objectives : This study was performed to investigate the effect of root extract of Pueraria thunbergiana Bentham (Puerariae Radix, PR) in diabetic mice as similar as emaciation-thirst disease in Oriental medicine. Methods : C57BL/6 mice were fed high fat (HF) and high sucrose (HS) for 8 weeks, and then administrated with 90 ㎎/㎏ body weight (bw) of streptozotocin (STZ) for induction of diabetes which is similar to the middle emaciation stage. After 5 days, blood glucose levels were measured, and selected the mice with ranges above 250 ㎎/㎗. PR water extract was administrated orally once a day for 4 weeks with high fat and high sucrose. The levels of glucose, insulin, total cholesterol, triglyceride, γglutamyl transpeptidase (γGTP), glutamic oxaloacetic transaminase (GOT) and glutamate pyruvate transaminase (GPT) were analysed in the serum. Also, observed their histological changes by hematoxylin and eosin (H&E) of different organs, lung, heart, pancreas, stomach, liver, and kidney. Results : PR extract significantly decreased the levels of serum glucose and insulin in diabetic mice. PR extract significantly increased the levels of triglyceride, total cholesterol, GOT and GPT in diabetic mice. In H&E stain, PR extract inhibited the histopathological changes of lung (as a channel of the upper emaciation stage in the channel-tropism theory), pancreas (as a channel of the middle emaciation stage) and kidney (as a channel of the lower emaciation stage) in diabetic damage. Conclusions : PR extract has an anti-diabetic effect in HF/HS and low-dose STZ-induced diabetic mice. This result suggests that PR follows the channel-tropism theory in the emaciation-thirst disease through the protection of lung, pancreas and kidney.

우슬 물추출물의 허혈성 뇌 손상에 대한 보호효과 연구

오태우 ( Tae Woo Oh ),박기호 ( Ki Ho Park ),이미영 ( Mi Young Lee ),최고야 ( Go Ya Choi ),박용기 ( Yong Ki Park ) 대한본초학회 2012 大韓本草學會誌 Vol.27 No.2

Objectives:This work was designed to investigate the effect of The root of Achyranthes japonica Nakai (AJN) water extract on serum deprivationreperfusion-induced apoptosis in PC-12 cells and transient middle cerebral artery occlusion (tMCAO)-induced ischemic brains of rats. Methods:Apoptosis in PC12 cells was induced by serum deprivation and reperfusion. The cells were treated with AJN water extract at doses of 0.5 and 1.0 ㎎/㎖for 24 hr after inducing the apoptosis. Cell viability was determined by WST-1 assay. The expression of caspase-3 protein was determined by Western blot. Ischemic brains were prepared from tMCAO-induced ischemic rats after oral administration with AJN at dose of 50 and 100 ㎎/㎏, and then brain infarction was measured by TTC staining. Results:AJN significantly increased the cell viability in apoptocic-induced PC-12 cells, and also decreased the expression of caspase-3 protein. Furthermore, the administration of AJN significantly inhibited tMCAO-induced brain infarction in rats. Conclusions:Our results suggest that AJN extract has a neuroprotective property via suppressing the apoptosis in PC12 cells and the infarction of ischemic brains.

허혈,재관류 유도성 신경세포사멸에 대하여 신경보호효과를 가지는 약용식물 추출물의 검색

오태우 ( Tae Woo Oh ),이미영 ( Mi Young Lee ),이혜원 ( Hye Won Lee ),박용기 ( Yong Ki Park ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.2

Objectives: The purpose of the study is to determine the neuroprotective effects of the water and 80% EtOH extract of some herbal medicine plant on ischemia·reperfusion-induced cell death in SK-N-SH human brain neuronal cells. Methods : SK-N-SH cells were treated with 3mM sodium azide and 10 mM 2-deoxy-D-glucose for 45 min, ptior to the addition of different concentrations of herbal medicine plant extract (0, 10, 25, 50, 100, 250, 500, 1000 μg/ml) for 2 hr and then reperfused with growth medium, incubated for 24 h. Cell viability was determined by WST-1 assay, and ATP/ADP levels were measured by ADP/ATP ratio assay kit. Results: Herbal medicine plant extract significantly inhibited decreasing the cell viability in ischemia-induced SK-N-SH cells. Also increased the ratio of ADP/ATP in ischemia-induced neuronal cells, Conclusions: Our results suggest that herbal medicine plant extract has a neuroprotective property via increasing the energy levels in neuronal cells, suggesting that extract may has a therapeutic potential in the treatment of ischemic brain injury. The exact component and mechanism remains for the future study.

Streptozotocin 저용량 반복투여로 유도한 당뇨 흰쥐에서 구기자 추출물의 효능연구

오태우 ( Tae Woo Oh ),박용기 ( Yong Ki Park ) 대한본초학회 2015 大韓本草學會誌 Vol.30 No.6

Objectives : This study was performed to investigate the anti-diabetic effect of the fruit extract of Lycium chinense Mill(Lycii Fructus, LF) on multiple low-dose streptozotocin-induced diabetic rats. Methods : Male Sprague-Dawley rats were divided into three groups; normal, STZ-control, Lycii Fructus extractorally administrated 300 ㎎/㎏ group (STZ-LF). Diabetes was induced in rats by consecutive injection of streptozotocin (STZ) at doses of 30 ㎎/㎏ for 5 days. After 4 weeks, all rats were sacrificed, Fasting blood glucose, total cholesterol (CHO), triglyceride (TG) and HDL-Cholesterol were measured in sera of rats. Histopathological changes of pancreas, kidney, liver and lung tissues were observed by microscope after H&E, Periodic acid-Schiff (PAS) and Masson``s trichrome staining. The changes of body weight, blood glucose, and food and water intake were also measured. Results : There were no differences in body, food intake and water intake in LF-administrated groups compared with STZ control group. However, LF extract significantly decreased the levels of serum glucose, CHO, TG and HDL-Cholesterol in diabetic rats. In histopathological analysis of kidney, liver and lung, LF-administrated groups showed the inhibition of morphological damage. Conclusions : These results suggest that LF have a biological action on multi low-dose STZ-induced diabetes in rats via decreasing the serum glucose, TG and TG levels and may protect the morphological changes of kidney, liver and lung.

멀티미디어 처리 : 센서 패턴 잡음을 이용한 디지털 영상 획득 장치 판별

오태우 ( Tae Woo Oh ),현대경 ( Dai Kyung Hyun ),김기범 ( Ki Bom Kim ),이해연 ( Hae Yeoun Lee ) 한국정보처리학회 2015 정보처리학회논문지. 소프트웨어 및 데이터 공학 Vol.4 No.12

IT 기술이 급격히 발전함에 따라서 디지털 멀티미디어 장치 및 소프트웨어를 이용한 콘텐츠가 범람하고 있다. 그러나 불법적 목적을 가지고 있는 사용자가 활용함에 따라 이를 이용한 범죄가 증가되고 있고 멀티미디어 포렌식을 통한 콘텐츠의 보호 및 불법 사용 차단의 필요성이 대두되고 있다. 본 논문에서는 센서 패턴 잡음을 이용하여 디지털 영상 획득 장치 판별을 위한 포렌식 기술에 대하여 제안한다. 먼저 광자 탐지기의 빛에 대한 민감도가 불완전해 생기는 센서 패턴 잡음을 검출하기 위한 기술에 대하여 제시한다. 그다음에 참조 영상들에 대하여 센서 패턴 잡음을 추정하고, 검사 영상에 대하여 센서 패턴 잡음을 추정한 후 두 잡음 사이의 유사성 계산을 통하여 디지털 영상을 획득한 장치에 대하여 판별하는 방법을 설명한다. 제안한 기술의 성능 분석을 위하여 DSLR 카메라, Compact 카메라, 스마트폰, 캠코더 등을 포함한 총 10대 장치에 대하여 개발한 알고리즘에 대한 정량적 성능의 분석을 수행하였고, 그 결과 99.6%의 판별 정확도를 달성하였다. With the advance of IT technology, contents from digital multimedia devices and softwares are widely used and distributed. However, novice uses them for illegal purpose and hence there are needs for protecting contents and blocking illegal usage through multimedia forensics. In this paper, we present a forensic technique for identifying digital imaging source using sensor pattern noise. First, the way to acquire the sensor pattern noise which comes from the imperfection of photon detector against light is presented. Then, the way to identify the similarity of digital imaging sources is explained after estimating the sensor pattern noises from the reference images and the unknown image. For the performance analysis of the proposed technique, 10 devices including DSLR camera, compact camera, smartphone and camcorder are tested and quantitatively analyzed. Based on the results, the proposed technique can achieve the 99.6% identification accuracy.

Streptozotocin 유도 당뇨 흰쥐에서 주요 장부 간 조직학적 변화 비교 연구

오태우 ( Tae Woo Oh ),강석용 ( Seok Yong Kang ),박용기 ( Yong Ki Park ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.6

Objectives : This study was conduct to compare of histological changes on four target organs which related with diabetes in streptozotocin-induced diabetic rats. Methods : Diabetes was induced in male Sprague-Dawley rats by consecutive injection of streptozotocin (STZ) at different doses of 30, 40 and 50 ㎎/㎏ for 5 days. After 4 weeks, all rats were sacrificed, five different organs such as pancreas, liver, kidney, and lung were isolated and observed their histological changes by hematoxylin and eosin (H&E), Periodic acid-Schiff (PAS) and Masson`s trichrome staining. The changes of body weight, blood glucose, and food and water intake were also measured. Results : The multiple administration of STZ was induced diabetes in rats with hyperglycemia, decrease of body weight, increase water and food intake, and histopathological changes of target organs, compared with those of normal rats in both dose-dependent and time-dependent manner. In histological analysis, pancreas was showed decrease of the islet numbers with beta-cell loss. Kidney showed morphological damage with glomerulus hypertrophy, and also lung was showed bronchial epithelial damage with inflammatory cells infiltration. In liver, the portal vein and hepatic artery could not observed, and showed inflammatory cell infiltration with liver fibrosis. Conclusions : These results suggest that the increase of the capacity of STZ, each of the more chronic disease, it can be seen that the damage was deep. Thus, evaluate the resulting drug appropriate depending on the purpose of the model is expected to be selected.

비트 인터리빙이 가능한 데이터 의존형 10T 정적 램 비트셀

오태우(Tae Woo Oh),정성욱(Seong-Ook Jung) 대한전자공학회 2015 대한전자공학회 학술대회 Vol.2015 No.6

허혈,재관류 유도 신경세포사멸에 대한 일당귀 물추출물의 신경보호효과 연구

오태우 ( Tae Woo Oh ),박기호 ( Ki Ho Park ),이미영 ( Mi Young Lee ),최고야 ( Go Ya Choi ),박용기 ( Yong Ki Park ) 대한본초학회 2011 大韓本草學會誌 Vol.26 No.4

Objectives: The purpose of the study is to determine the neuroprotective effects of the water extract of Angelicae Acutilobae Radix(AA) on ischemia·reperfusion-induced apoptosis in SK-N-SH human brain neuronal cells. Methods: SK-N-SH cells were treated with different concentrations of AA water extract (0.1, 0.2, 0.5 and 1.0 mg/ml) for 2 hr and then stimulated with Dulbecco`s phosphate-buffered saline containing CI-DPBS: 3mM sodium azide and 10 mM 2-deoxy-D-glucose for 45 min, reperfused with growth medium, and incubated for 24 h. Cell viability was determined by WST-1 assay, and ATP/ADP levels were measured by ADP/ATP ratio assay kit. The levels of caspase-3 protein were determined by Western blot and apoptotic body was observed by Hoechst 33258 staining. Results: AA extract significantly inhibited decreasing the cell viability in ischemia-induced SK-N-SH cells. AA also increased the ratio of ADP/ATP in ischemia-induced neuronal cells and decreased the expression levels of apoptotic protein, caspase-3 and apoptotic DNA damage. Conclusions: Our results suggest that AA extract has a neuroprotective property via suppressing the apoptosis and increasing the energy levels in neuronal cells, suggesting that AA extract may has a therapeutic potential in the treatment of ischemic brain injury.

Streptozotocin으로 유도된 당뇨 흰쥐에서 하소에 사용되는 한약의 항당뇨 효과 비교 연구

오태우 ( Tae Woo Oh ),강석용 ( Seok Yong Kang ),김경호 ( Kyung Ho Kim ),송미영 ( Mi Young Song ),박용기 ( Yong Ki Park ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.5

Objectives: To prove the channel-tropism theory in herbology, we investigated the anti-diabetic effect of six herbal plants used for lower wasting-thirst in streptozotocin-induced diabetic rats. Methods: Diabetes was induced in male Sprague-Dawley rats by consecutive injection of streptozotocin (30 mg/kg i.p.) for 5 days. The rats were divided into normal control, diabetic control, and diabetic treatment with Lycii Radicis Cortex (LRC, 300 ㎎/㎏); Corni Fructus (CF, 300 ㎎/㎏); Bombyx Batryticatus (BB, 50 ㎎/㎏); Lycii Fructus (LF, 300 ㎎/㎏); Phellodendri Cortex (PC, 300 ㎎/㎏); Epimedii Herba (EH, 300 ㎎/㎏); and glibenclimide (10 ㎎/㎏) as a reference drug. Herbal extracts or reference drug were administered orally for 28 days. The changes of body weight, food intake and water intake, and serological markers such as blood glucose, serum total cholesterol, triglyceride (TG), blood urea nitrogen (BUN), and creatinine (Cr) were measured. Results: The decrease of body weight and the increase of food and water intake in STZ-induced diabetic rats was improved by the administration of CF and LF. Also, the enhancement of blood glucose and serum total cholesterol, TG, BUN and Cr in STZ-induced diabetic rats was significantly inhibited by the administration of CF, BB, LF and glibenclimide. On the other hand, EH strongly inhibited the increase of BUN and Cr in the sera of STZ-induced diabetic rats. Conclusions: These results suggest that among six herbal medicines used lower emaciation of emaciation-thirst disease, CF, BB, LF and EH show a characteristics including the channel-tropism theory.