OFDM 수신기를 위한 주파수 동기화 기법

오지성,정영모,이상욱 한국방송공학회 1995 한국방송공학회 학술대회 Vol.1 No.1

This paper proposes a new frequency offset correction technique for OFDM receivers oa a frequency-selective fading channel. The frequency offset in the OFDM signals is known to introduce an interchannel interference among the multiple subcarries, which degrades the receiver performance severely. In order to reduce the frequency offset, this paper describes an algorithm with two stages: acquistion and tracking. At both stages, the algorithm oversamples the received OFDM signals. At the acquisition stage the frequency offset is reduced to half or less than the intercarrier spacing by matching the sign patterns of even and odd samples. Next, at tracking stage the frequency offset is compensated by a frequency detector which is controlled by the correlation of the even and odd sample sets. From the results, it is found that the proposed algorithm can correct the frequency offset even if the initial offset exceeds one half of the intercarrier spacing.

Pseudoalteromonas donghaensis HJ51의 체외 단백질 분해효소 특성과 생산 조건

오지성,최윤수,노동현,Oh, Ji-Sung,Choi, Yoon-Soo,Roh, Dong-Hyun 한국미생물학회 2015 미생물학회지 Vol.51 No.1

동해에서 분리된 Pseudoalteromonas donghaensis HJ51는 체외분비 단백질 분해효소를 생산하는 신종 미생물로 보고되었다. 체외 단백질 분해효소의 특성과 최적 생산 조건을 결정하기 위해 crude supernatant을 사용하여 단백질 분해효소의 최적 활성 온도와 pH를 조사한 결과 $40^{\circ}C$와 pH 7.5-10.5이었으며, pH 11에서도 88%의 높은 상대적인 효소 활성을 나타내었다. 효소의 금속 요구성을 조사한 결과, $Fe^{3+}$를 10 mM로 첨가하였을 때 최대 효소활성 증가를 보였다. 최대의 효소생산 조건을 탐색한 결과, 기본배지인 PY-ASW (peptone 0.5%, yeast extract 1.0%, artificial seawater)에 탄소원을 첨가하지 않는 것이 가장 높았으며, 질소원으로는 peptone 보다 beef extract, tryptone, casmino acids을 각각 첨가했을 때 활성이 21, 7, 4% 증가하였다. 이러한 결과를 종합해 볼 때 P. donghaensis HJ51이 생산하는 효소는 알칼리성 pH 환경 및 저온환경에서 활성이 필요한 분야에 응용이 가능할 것으로 사료된다. Pseudoalteromonas donghaensis HJ51, isolated from the East Sea, has been reported as a novel strain to produce extracellular protease. Crude supernatant was used to determine optimal activity and optimal production conditions for the enzyme. It was found that the optimal temperature and pH of the protease were $40^{\circ}C$ and pH 7.5-10.5, respectively. The enzyme activity was kept to 88% at the pH 11. In metal requirement analysis, the enzyme exhibited the highest activity when 10 mM $Fe^{3+}$ was supplied. While supplementation of additional carbon sources used in study showed no positive effect on cell growth and enzyme activity, the addition of beef extract, tryptone, or casamino acids instead of peptone of PY-ASW containing 1% glucose increased enzyme production to 21, 7, 4%, respectively. Taken together these properties, the enzyme produced from P. donghaensis HJ51 can be applied to the industries that require protease activity under alkaline pH and low temperature.

Draft genome sequence of an Alteromonadaceae bacterium BrNp21-10 isolated from seawater

오지성,노동현 한국미생물학회 2023 미생물학회지 Vol.59 No.4

The draft genome sequence of an Alteromonadaceae bacterium BrNp21-10 isolated from coastal seawater was determined using the Illumina Hiseq X-ten platform. The assembled genome consists of 33 contigs totaling 4,217,876 bp with N50 values of 212,777 bp. The genomic DNA G + C content was 42.5%. The draft genome comprised 3,674 protein-coding, 5 rRNA, 48 tRNA, 4 non-coding RNA genes, and 15 pseudogenes. There were a series of genes related to CRISPR, flagellar assembly, and assimilatory sulfate reduction in the genome. The isolated strain BrNp21-10 is considered to be a novel strain belonging to the family Alteromonadaceae by 16S rRNA gene sequence and digital DDH analysis.

Draft genome sequence of a Flavobacterium chungbukense CS100T isolated from soil

오지성,노동현 한국미생물학회 2022 미생물학회지 Vol.58 No.4

The draft genome sequence of Flavobacterium chungbukense CS100T isolated from soil was determined using Illumuna Hiseq X-ten platform. The assembled genome consists of 18 scaffolds with a total length of 5,355,850 bp with N50 values of 851,609 bp. The genomic DNA G + C content was 33.5%. The draft genome encoded 4,436 protein-coding genes, 8 rRNA genes, 52 tRNA genes, 3 non-coding RNA genes and 53 pseudogenes. The genome sequence of this type strain in the genus Flavobacterium will be used for reference to taxonomical classification based on the genome. Additionally, several useful genes in the genome related to antimicrobials, moisturizer and pigments production, and the degradation of aromatic compounds and biopolymers might be used for industrial applications.

Flavobacteriaceae 과에 속하는 ‘Aurantibacter crassamenti ’ KCTC 52207의 유전체 염기서열 분석

오지성,노동현 한국미생물학회 2021 미생물학회지 Vol.57 No.3

Here we report draft genome sequence of ‘Aurantibacter crassamenti’ KCTC 52207, belonging to the family Flavobacteriaceae. Its draft genome sequence was determined using Illumuna Hiseq X-ten platform. The assembled genome of ‘A. crassamenti’ KCTC 52207 consisted of two contigs with a total length of 4,519,628 bp and the genomic DNA G + C content was 34.3 mol%. The draft genome encoded 3,739 protein-coding genes, 6 rRNA genes, 38 tRNA genes, 4 non-coding RNA genes, and 8 pseudo genes. The genome contained the genes related to the decomposition of carbohydrates and xenobiotics biodegradation in the marine environments, a characteristic of the family Flavobacteriaceae. 본 연구에서 Flavobacteriaceae 과에 속하는 ‘Aurantibacter crassamenti’ KCTC 52207의 유전체를 분석하였다. 유전체 서열은 Illumina Hiseq X-ten platform을 사용하여 결정하였 다. ‘A. crassamenti’ KCTC 52207의 조립된 유전체는 2개의 contig로 구성되었고, 총 길이는 4,519,628 bp이고, 34.3 mol% 의 G + C 함량을 보유하였다. 유전체는 3,739개의 단백질 코딩 유전자, 6개의 rRNA 유전자, 38개의 tRNA 유전자, 4개의 non-coding RNA 유전자 및 8 위유전자 (pseudo gene)를 암호 화하였다. 유전체에는 해양 환경에서 Flavobacteriaceae 과의 특징인 탄수화물과 xenobiotics 분해에 관련된 유전자를 포함 하고 있었다.

Draft genome sequence of Zhongshania marina DSW25-10^T isolated from seawater

오지성,노동현,Oh, Ji-Sung,Roh, Dong-Hyun The Microbiological Society of Korea 2018 미생물학회지 Vol.54 No.4

이 연구에서는 Illumina Hiseq platform을 사용하여 동해 심층 해양수로부터 분리된 Zhongshania marina $DSW25-10^T$의 유전체 염기서열 해독을 수행하였다. 그 결과, 유전체는 대략 4.08 Mbp의 길이 및 49.0%의 G + C 함량으로 구성되었고, 전체 3,702개의 단백질 암호 유전자, 3개의 rRNA 유전자, 39개의 tRNA 유전자, 4개의 non-coding RNA 유전자 및 36개의 위 유전자(pseudogenes)가 확인되었다. 또한, 지방족 및 방향족 화합물의 대사 경로가 확인되었다. 이러한 대사 경로들로 비추어 Zhongshania marina $DSW25-10^T$는 유용한 생물 정화 자원으로 사용될 수 있을 것으로 기대된다. The draft genome sequencing for Zhongshania marina $DSW25-10^T$, isolated from deep seawater of East Sea in Korea, was performed using Illumina HiSeq platform. As a result, the draft genome was comprised of a total length of approximately 4.08 Mbp with G + C content of 49.0%, and included a total of 3,702 protein-coding genes, 3 rRNA genes, 39 tRNA genes, 4 non-coding RNA genes, and 36 pseudogenes. In addition, the metabolic pathways of aliphatic and aromatic compounds were identified. In light of these metabolic pathways, Zhongshania marina $DSW25-10^T$ is expected to be a useful bioremediation resource.

살조성 세균 Halobacillus sp. Nhm2S1의 유전체 염기서열 분석

오지성,노동현 한국미생물학회 2021 미생물학회지 Vol.57 No.3

An algicidal bacterium, designated strain Nhm2S1, was isolated from tidal flat of South Sea, Korea. Herein, we report draft genome sequence of strain Nhm2S1, which was determined using Illumina HiSeq X-ten platform. The assembled genome of strain Nhm2S1 consists of 10 contigs with a total length of 3,926,919 bp and the genomic DNA G + C content was 43.4 mol%. The draft genome encoded 3,876 protein-coding genes, 11 rRNA genes, 65 tRNA genes, 4 non-coding RNA genes and 26 pseudo genes. The genome contained genes (redP/R) involved in the biosynthesis of the algicidal pigment prodigiosin, which was thought to helpful in understanding of algae-killing properties. 살조성 세균 Nhm2S1 균주는 남해의 갯벌로부터 분리되었 다. 본 연구에서는 Illumuna Hiseq X-ten platform을 사용하여 Nhm2S1 균주의 유전체 서열을 수행하였다. Nhm2S1 균주의 조립된 유전체는 10개의 contig로 구성되었고, 염색체 길이는 3,926,919 bp이며 43.4 mol% G + C 함량을 지니고 있었다. 유 전체는 3,876개의 단백질 암호 유전자, 11개의 rRNA 유전자, 65개의 tRNA 유전자, 4개의 non-coding RNA 유전자 및 26 위 유전자(pseudo gene)를 암호화하였다. 게놈에는 살조성 색소 프로디지오신의 생합성에 관여하는 유전자(redP/R)가 포함 되어 있어 살조 특성을 이해하는 데 도움이 될 것이다.

소형 하이브리드 로켓의 시험 발사 및 고도 측정

오지성,신준수,문근환,이정표,문희장,성홍계,김진곤,Oh, Ji-Sung,Shin, Jun-Su,Moon, Keun-Hwan,Lee, Jung-Pyo,Moon, Hee-Jang,Sung, Hong-Gye,Kim, Jin-Kon 항공우주시스템공학회 2010 항공우주시스템공학회지 Vol.4 No.3

In this study, small hybrid rocket was launched and flight altitude was measured. Flight path of rocket was analyzed with exterior ballistics analysis result. The data acquisition equipment was loaded in rocket and two launch test were performed. HDPE was used as fuel and N20 as oxidizer for this study.

Complete genome sequence of Pseudoalteromonas donghaensis HJ51^T isolated from seawater

오지성,노동현,Oh, Ji-Sung,Roh, Dong-Hyun The Microbiological Society of Korea 2018 미생물학회지 Vol.54 No.3

이 연구에서는 PacBio RS II 플랫폼을 사용하여 동해에서 분리된 해양 미생물 Pseudoalteromonas donghaensis $HJ51^T$의 전체 유전체 서열화를 수행하였다. 그 결과, 크기 3,646,857 bp, G + C 함량 41.8%인 염색체와 함께 크기 842,855 bp, G + C 함량 41.3% 및 크기 244,204 bp, G + C 함량 40.4%인 두 개의 플라스미드로 구성된 3개의 유전체 서열을 획득하였다. 이 유전체는 4,083개의 단백질 암호화 유전자와 127개의 RNA 유전자를 포함하고 있다. 다양한 생체 고분자 분해효소의 유전자원과 대장균과 유사한 세포외 단백질 분비 숙주로서의 개발에 이용될 수 있을 것으로 생각된다. The whole genome sequencing using PacBio RS II platform was performed for a marine bacterium Pseudoalteromonas donghaensis $HJ51^T$ isolated from East Sea of Korea. As a result, three assembled contigs consisting of a chromosome (size of 3,646,857 bp, and G + C content of 41.8%) and two plasmids (size of 842,855 bp and 244,204 bp, and G + C content of 41.3% and 40.4%, respectively) were obtained. The genome included 4,083 protein coding genes and 127 RNA genes. This result could be used for gene sources of biopolymers degradation and the development as a new host with secretion system similar to Escherichia coli.

Draft genome sequence of Pelagicola sp. DSW4-44 isolated from seawater

오지성,노동현,Oh, Ji-Sung,Roh, Dong-Hyun The Microbiological Society of Korea 2019 미생물학회지 Vol.55 No.3

이 연구에서는 Illumina Hiseq platform을 사용하여 동해 심층 해양수로부터 분리된 Pelagicola sp. DSW4-44 (= KCTC 62762 = KCCM 43261)의 초안 유전체 염기서열 해독을 수행하였다. 그 결과, 유전체는 대략 4.85 Mbp의 길이 및 54.3%의 G + C 함량으로 구성되었고, 전체 4,566개의 단백질 암호 유전자, 3개의 rRNA 유전자, 48개의 tRNA 유전자, 3개의 non-coding RNA 유전자 및 67개의 위유전자(pseudo gene)가 확인되었다. 초안 유전체에서 균주 DSW4-44는 Pelagicola 속의 다른 균주에서 발견되지 않는 이화적 질산염의 암모늄 환원과 탈질화의 질소대사 유전자를 가지고 있었다. The draft genome sequencing for Pelagicola sp. DSW4-44 (= KCTC 62762 = KCCM 43261), isolated from deep seawater of East Sea in Korea, was performed using Illumina HiSeq platform. As a result, the draft genome was comprised of a total length of approximately 4.85 Mbp with G + C content of 54.3%, and included a total of 4,566 protein-coding genes, 3 rRNA genes, 48 tRNA genes, 3 non-coding RNA genes, and 67 pseudo genes. In the draft genome, the strain DSW4-44 contained genes involved in the nitrogen metabolism of dissimilatory nitrate reduction to ammonium (DNRA) and denitrification, which were not found other strains in the genus Pelagicola.