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While the amount of sensor data has been rapidly increasing due to the advances in information technology, wireless internet and sensor technologies, it is becoming increasingly necessary to be able to support the collection and analysis of such sensor data. Especially the development and dissemination of device control boards such as Arduino or Rasberry Pi have enabled the easy installment of various sensors. However, it is too difficult for people with non-computer related backgrounds or beginners to use them to perform data collection. Furthermore, even though the data is collected, performing data analysis and visualization tasks could be difficult without expert knowledge and experience. To overcome these issues, our goal is to construct a library for collecting and analyzing sensor data. In order to simplify the data collection process we construct modules for each sensor, and make it possible to collect data by easily calling the sensor functions in the modules. For the data analysis and visualization process we defined function names as general words to construct a more understandable library, and we reduced the number of function arguments which are used in a function call to minimize the information needed to perform the data analysis and visualization. The sensor data collection part is developed using the C programming language, and the sensor data analysis part is developed using the R programming language. 정보 기술과 무선 인터넷 및 센서 기술의 발달과 더불어 센서 데이터의 생성이 급속하게 증가하고 있는 가운데 이들 센서 데이터의 수집과 분석을 효율적으로 지원하기 위한 필요성이 커지고 있다. 특히 아두이노와 라즈베리파이 같은 보드들의 보급으로 손쉽게 다양한 센서를 직접 설치할 수 있게 되었지만 보드를 이용한 데이터 수집에는 컴퓨터 관련 전공자가 아니거나 처음 접해보는 경우 사용하는 데 있어서 많은 어려움이 있다. 또 데이터를 수집하더라도 전문적인 지식이 부족하거나 경험이 없으면 데이터 분석 및 시각화를 하는데 어려움이 발생할 수 있다. 본 논문에서는 이러한 문제점을 보완하는 센서 데이터 수집 및 분석을 위한 라이브러리구축을 목표로 한다. 데이터 수집 과정을 간소화하기 위해 각 센서 별로 모듈을 구축하였고, 간단하게 원하는센서 함수를 호출하면 데이터 수집이 가능하게 하였다. 또 데이터 분석 및 시각화 과정에서는 사용자가 직관적으로 이해할 수 있는 함수의 이름을 사용하여 함수를 정의하고, 함수에 들어가는 인자들을 간소화하여, 최소한의 정보로 데이터 분석 및 시각화를 가능하도록 설계하였다. 센서 데이터 수집은 C 언어를 기반으로 구축하였으며, 데이터 분석 및 시각화 과정을 R 프로그래밍 언어를 기반으로 구축하였다.
This paper aimed to verify the protective effects of Thuja orientalic extract against oxidative stress caused by H2O2 Rotenone and Paraquat in the keratinocyte(HaCaT cell) of human-derived normal skin for the development of safer health food or functional cosmetic materials. It has been reported that Thuja orientalic has antioxidant effects. In accordance with the cytotoxicity of Thuja orientalic extract against HaCaT cells using MTT, the appropriate concentration was less than 50㎍/mL. Thuja orientalic extract exhibited protective effects against H2O2, rotenone, and paraquat by 86.6%, 86.45%, and 60.24%, respectively, at the concentration of 50㎍/mL. Thuja orientalic extract has protective effects against all three artificial stresses, and more protective effects against H2O2 and rotenone. The analysis results indicated that Thuja orientalic extract had antioxidant effects that could offer protection to the cells from a variety of oxidative stresses. Accordingly, Thuja orientalic extract has a highly applicable value as a functional health food or cosmetic ingredient in the current times in which wellbeing is a priority.
Purpose: The development of an efficient in vitro cell culture device to process various cells would represent a majormilestone in biological science and engineering. However, the current conventional macro-scale in vitro cell cultureplatforms are limited in their capacity for detailed analysis and determination of cellular behavior in complex environments. This paper describes a microfluidic-based culture device that allows accurate control of parameters of physical cues such aspressure. Methods: A microfluidic device, as a model microbioreactor, was designed and fabricated to culture Saccharomycescerevisiae and Chlamydomonas reinhardtii under various conditions of physical pressure stimulus. This device wascompatible with live-cell imaging and allowed quantitative analysis of physical cue-induced behavior in yeast andmicroalgae. Results: A simple microfluidic-based in vitro cell culture device containing a cell culture channel and an airchannel was developed to investigate physical pressure stress-induced behavior in yeasts and microalgae. The shapes ofSaccharomyces cerevisiae and Chlamydomonas reinhardtii could be controlled under compressive stress. The lipid productionby Chlamydomonas reinhardtii was significantly enhanced by compressive stress in the microfluidic device when comparedto cells cultured without compressive stress. Conclusions: This microfluidic-based in vitro cell culture device can be used asa tool for quantitative analysis of cellular behavior under complex physical and chemical conditions.
Degradation of chlorophyll, carotenoids, andphycobilins in dried laver (Porphyra) was studied duringstorage at water activities (Aw) of 0.112, 0.316, 0.484,0.747, or 0.890 in the dark at 40oC for 15 days. Thechlorophyll, carotenoid, and phycobilin contents weredetermined using HPLC and spectrophotometry. Thechorophyll a, carotenoid, and phycobilin contents in driedlaver decreased with storage time in the dark, anddegradation was increased and accelerated as the Aw valueincreased. Among pigments, chlorophyll a was degraded atthe highest rate, and differences in degradation rates amongpigments became greater as the Aw value increased. Phycoerythrin was more stable than phycocyanin. Changesin the Aw value affected degradation of phycocyanin andchlorophyll more than phycoerythrin or carotenoids. Control of the Aw value can improve the color stability ofdried laver in the dark.
Effects of light on lipid oxidation, antioxidants,and pigments in dried laver (Porphyra) were studiedduring storage at 40oC and at a water activity of 0.75 for 15days. Lipid oxidation was evaluated by measuring peroxidevalue (POV) and conjugated dienoic acid (CDA) contents,whereas fatty acid composition was analyzed by gaschromatography. Contents of polyphenols, tocopherols,porphyran, chlorophylls, carotenoids, and phycobilins werealso monitored. The POV and CDA contents of dried laverlipids increased during storage, whereas contents ofeicosapentaenoic acid, pigments, and antioxidants decreased. Light accelerated lipid oxidation as well as degradation ofantioxidants and pigments during storage of dried laver. Chlorophyll and polyphenols were the most rapidly degradedamong all pigments and antioxidants, respectively, andlight had the strongest effect on their degradation. Lipidoxidation of dried laver due to light was highly dependenton the content of α-tocopherol among all minor compounds.
This study aims to discover a possibility that ethanol and methanol extracts of dried and fermented Salicornia herbacea can be used as materials of cosmetics through bioactivity such as cytotoxicity, whitening activity, and antioxidation. As a result of the cytotoxicity which was carried targeting Raw 264.7 cell leave, it was discovered that there was no cytoxicity from the four extracts as cell survival rates of all the extracts were 106~133%. When the tyrosinase inhibitory activity for whitening was measured, all the four extracts had high inhibitory activity according to concentration and the inhibitory activity of ethanol extract of fermented S. herbacea was the highest. When DPPH radical scavenging effect for antioxidation was measured, the scavenging effect of ethanol extract of fermented S. herbacea was the highest. In particular, in comparison with antioxidative effect of BHT SC50 of 1.29 ㎎/㎖, that of fermented S. herbacea was similar, which indicates that it has a relatively high antioxidative effect. Therefore, as it was demonstrated that S. herbacea, methanol and ethanol extracts of fermented S. herbacea had no toxicity as materials of cosmetics and of the four specimens, ethanol extract of fermented S. herbacea has the highest in cytotoxicity, tyrosinase inhibitory effect and DPPH radical scavenging effect, its possibility to be used as a material of fermented cosmetics was confirmed.