Reduced Glutathione의 In Vitro 첨가(添加)가 마우스간조직(肝組織)의 내재(內在) NP-SH 및 NP-SS에 미치는 영향(影響)

오상유,Oh, Sang-You 대한생리학회 1973 대한생리학회지 Vol.7 No.2

In view of the recent knowledge on the radioprotective action of reduced glutathione (GSH), the present study was designed the elucidate the effect of some concentrations of GSH on the levels of intrinsic non-protein sulfhydryl (NP-SH) and non-protein disulfide (NP-SS) of the mouse liver incubated at 4, 25 and 37C in vitro, respectively. The liver slice of the mouse was incubated at 4, 25 and 37C in the medium composed of 100 ml of Krebs-Ringer phosphate buffer (KRP) with the addition of 10, 20 and 30 mg of GSH, respectively. Measurement of NP-SH and NP-SS was made at 5, 30 and 60 min during the course of the incubation, and the results were compared with the controls which were incubated only in KRP medium, and the normal. The results thus obtained are summarized as follows: 1. When the mouse liver slice was incubated at 4C, the values of both NP-SH and NP-SS of the control and the group where 10 mg of GSH was added to the incubation medium were similar to those of the normal group, and the increase of NP-SH and NP-SS with the increased concentrations of GSH was not prominent. 2. When the liver slice was incubated in the concentrations of GSH 20 mg/100 ml KRP and GSH 30 mg/100 ml KRP at 25 C, the rate of increase of both NP-SH and NP-SS was proportional to the increase of GSH concentration. In the group where 10 mg of GSH was added to the incubation medium, the value of NP-SH and NP-SS reached the highest value at 30 min, but a tendency of decrease was observed at 60 min. 3. The rate of increase of NP-SH and NP-SS of the liver was most marked of all the group. studied when the incubation temperatuse was elevated to 37C, and the increase was proportional to the concentration of GSH and the incubation time. 마우스 간조직(肝組織)을 수종(數種) 농도(濃度)의 GSH 용액중(溶液中)에서 각각(各各) 4 C, 25 C 및 37 C의 온도(溫度)속에서 incubate하고, 5분(分), 30분(分) 및 60분(分)에서 각각(各各) NP-SH 및 NP-SS의 양(量)을 정량(定量)하여 정상(正常) 및 대조군(對照群)의 그것들과 비교(比較)한 결과(結果) 다음의 결과(結果)를 얻었다. 1) 4 C에서 incubate한 군(群)에서는 대조군(對照群) 및 GSH 10 gm/100 ml KRP의 농도(濃度)에서 대체(大體)로 정상치(正常値)에 비(比)하여 대차(大差)없는 NP-SH 및 NP-SS를 나타내고 GSH의 농도(濃度)가 높아져도 SH 기(基)의 증가(增加)는 현저(顯著)치 않았다. 2) 25 C에서 incubate한 군(群)에서의 NP-SH및 NP-SS는 GSH 20 mg/100 mg KRP 및 GSH 30 mg/100 mg KRP 의 양군(兩群)에서 GSH의 농도(濃度)와 시간경과(時間經過)에 비례(比例)해서 증가(增加)하였고, GSH 10 mg/100 ml KRP 군(群)에서는 incubation 30분(分)에서 최고치(最高値)를 나타내고 60분(分)에서는 오히려 약간(若干) 저하(低下)되는 경향(傾向)을 나타내었다. 3) 37 C에서 incubate 한 군(群)에서는 GSH 농도(濃度)와 incubation의 시간(時間)에 비례(比例)해서 NP-SH 및 NP-SS가 대체(大體)로 증가(增加)하고 증가(增加)의 율(率)도 가장 현저(顯著)하였다.

천식 의심 환자에서 기관지 확장제 반응 및 만니톨 기도과민성과 호기산화질소의 연관성

오상유 ( Sang-yu Oh ),이민형 ( Min-hyung Lee ),오다은 ( Da-eun Oh ),최길순 ( Gil-soon Choi ),김석현 ( Seok-hyeon Kim ),박영찬 ( Young-chan Park ),김희규 ( Hee-kyoo Kim ) 대한내과학회 2017 대한내과학회지 Vol.92 No.5

목적: 호기산화질소(FeNO)는 천식에서 기도 염증의 비침습적 지표로서 활용되어 왔다. 또한, 호기산화질소(FeNO) 측정은 천식 평가에 유용한 도구로 인식되고 되고 있다. 이 연구의 목적은 천식 의심 환자에서 기관지확장제 반응(BDR) 및 만니톨 기도 과민성(AHR) 여부에 따른 FeNO의 차이를 알아보고자 하였다. 방법: FeNO를 측정한 기관지 천식 의심 환자들(≥ 13세)중 BDR 및 AHR 검사를 시행한 대상자들 두 군으로 구분하였고 각 군에서의 나이, 폐활량, 호산구, 혈청 총 IgE, 아토피 상태 등을 수집하였다. 또한, 각 FEV1 및 FEF25-75%을 기준으로 하여 각 검사 결과에 따라 FeNO 값을 비교 및 상관분석을 시행하였고 검사 양성을 예측하기 위한 ROC 곡선을 구하였다. 결과: BDR 검사군 124명, 만니톨 AHR 검사군 145명으로 총 259명의 환자들을 분석하였고 양 군의 평균 나이는 각각 52.8세와 35.3세였다. 환자의 BDR 여부, AHR 여부, 아토피여부, 혈액 호산구 분율(4% 기준)과 총 IgE 값(300 IU/mL 기준)에 따른 FeNO 값은 유의한 차이가 있었다. 또한, BDR 및 AHR 검사에서 FEV1의 기관지 확장제 사용 후 최종 증가 정도 및 PD15 값과 FeNO와의 상관관계가 FEF25-75%의 최종 증가 정도 및 PD15 값보다 더 높았다. BDR 및 AHR 양성을 예측하기 위한 위한 최적의 FeNO 결정값은 각각 38.5와 29.5ppb였다. 결론: 천식 의심 환자에 있어 FeNO는 BDR 및 AHR과 연관이 있었으며 양성 여부를 예측할 수 있는 보조적인 진단역할에 도움이 될 것으로 보인다. Background/Aims: Exhaled nitric oxide (NO) has been extensively investigated as a marker of airway inflammation in asthma, and fractional exhaled nitric oxide (FeNO) is recognized as a useful tool for its evaluation. The aim of this study was to investigate the relationships between FeNO levels and bronchodilator response (BDR), and between FeNO and mannitol-induced airway hyperresponsiveness (AHR), in patients with suspected asthma. Methods: Clinical variables were collected from patients aged ≥ 13 years with suspected bronchial asthma and measured levels of FeNO. These levels were compared with patient values for forced expiratory volume in the first second (FEV1) and forced expiratory flow at 25 and 75% of the pulmonary volume (FEF_25-75%) in bronchodilator response tests under control conditions, and during bronchial provocation with mannitol. Correlations and receiver operating characteristic (ROC) curves between FeNO levels and each test were assessed. Results: A total of 259 patients were included in the analysis. The mean ages of the two test groups were 41.1 and 47.8 years, respectively. FeNO levels were strongly correlated with bronchodilator response (%) and with the mannitol dose producing a 15% fall in FEV1 (PD15). On the other hand, FeNO levels were only weakly correlated with FEF_25-75%. The optimal cut-off values for FeNO to predict a positive BDR and AHR were 38.5 and 29.5 parts per billion, respectively. Conclusions: This study suggests that FEV1 and FEF_25-75% airway responses correlate with FeNO levels in patients with suspected bronchial asthma. FeNO levels may help to predict positive responses to BDR and AHR. (Korean J Med 2017;92:458-466)

S-222 요골동맥을 통한 심혈관 조영술 시행 후 발생한 핵간안근마비 1예

이정석,이종윤,오상유 대한내과학회 2016 대한내과학회 추계학술대회 Vol.2016 No.1

배경: 심혈관 조영술은 심혈관 질환의 진단에 있어서 중요한 검사 중 하나이며, 시행 횟수의 증가에 따른 합병증의 종류도 다양하게 보고 되고 있다. 저자들은 심혈관 조영술 시행 후 안근신경마비 증상이 나타난 예를 경험하였기에 보고하는 바이다. 증례: 49세 남자가 내원 수년전부터 간간히 발생하는 흉부불편감으로 외래 내원하여 심혈관 조영술 시행 후 현훈, 오심, 복시 호소하여 검사 당일 응급실 경유 입원하였다.1년전부터 고혈압으로 경구약 복용중이었고 특이 가족력은 없었고 흡연력 및 음주력은 없었다. 심혈관 조영술에서 좌전하행지의 심근가교 소견 보였으며 입원 당시 생체 징후는 안정적이었다. 뇌 자기공명 영상 및 자기공명 혈관촬영에서 급성뇌병변은 관찰되지 않았다. 비디오 안구운동검사상 우측방 주시에서 좌안의 단속성 운동이상 소견 및 왼쪽 3번 뇌신경의 신경다발 병변 소견 보였다. 환자는 4주간 보존적 치료 시행한 후 증상 호전보이고 비디오 안구운동 검사상 여전히 좌안 운동범위 감축소견 보이나 이전 결과와 비교했을 때 호전보여 퇴원하였다. 고찰: 심혈관 조영술 시행 후 급성 신경학적 합병증은 0.03%~0.3% 정도로 매우 드물며 증상은 신경손상 부위에 따라 다양하게 나타날 수 있다. 본 증례에서는 좌측 핵간안근마비(Internuclear ophthalmoplegia)에 부합하며 그 원인으로 뇌혈관 미세색전과 조영제에 의한 뇌신경 독성등을 생각해 볼 수 있다. 뇌혈관 미세색전은 하행대동맥이나 대동맥궁의 죽상경화반 일부가 떨어져 나와 발생할 수 있으며 대퇴동맥을 통한 심혈관 조영술에서 색전이 더 많이 발생할 수 있다. 조영제에 의한 신경학적 합병증은 드물며 혈액뇌장벽의 취약한 부분에서 누출되어 신경손상을 일으킬 수 있다. 경련, 혼란, 일시적인 대뇌피질맹 등의 증상이 나타날 수 있으며 일반적으로 보존적 치료를 한다.

一酸化炭素中毒 家兎의 肝, 腎組織 및 赤血球의 酸素 消費量에 대하여

申基棟,李圭源,吳相由,李東一,申鉉鑽 최신의학사 1972 最新醫學 Vol.15 No.2

In an attempt to observe a possible effect of CO intoxication on the oxygen consumption rates (Q02) of liver, kidney and red blood cell, the rabbit was exposed to either 0.2% CO gas for six hours or 0.1 CO gas for two hours daily for five days. The Q02 of the liver, kidney and red blood cell was measured by the Warburg's standard manometric method, and a comparison was made with the control. The results obtained are summarized as follows: 1. The Q02 of the liver, kidney and red blood cell in the normal rabbit was 1.82±0.3111 02/hr. /mg. D. W., 4.67±0.57 pl 02/hr. /mg. D. W. and 4.40 ? 10'9 pl 02/hr. /RBC, respectively. 2. In the group where the rabbit was exposed to 0.1% CO gas for two hours daily for five days, the QO2 of the liver, kidney and red blood cell was 1.41±0.24, 4.62±0.54 and 4.12.10-9, respectively. 3. The QOz of the liver, kidney and red blood cell in the 0.2% CO exposed group was 1. 75±0. 24, 4.70±0.72 and 4.10. 10'9; respectively. 4. From the above, it may be concluded that CO gas as used in this study do not produce a significant change in Q02 of the some organs from the control.

폐내압증가시(肺內壓增加時)의 동맥혈압(動脈血壓) 및 심박변동(心搏變動)에 관(關)하여

김규수(Kim, Kyu-Soo),오상유(Oh, Sang-Yu),최병옥(Choi, Byung-Ok),이중길(Lee, Joong-Kil) 대한생리학회 1969 대한생리학회지 Vol.3 No.2

Effects of graded increase of positive lung inflation upon heart rates and arterial blood pressure were observed in the anesthetized dogs to analyze the mechanical and neural regulatory factor in response to the positive inflation of the lung. The results obtained were summarized as followings: 1) When the low grade of positive lung inflation was employed under the mild to moderate anesthesia, central venous pressure was linearly increased while heart rate was decreased. After bilateral vagotomy, central venous pressure was obviously increased while heart rate was constant. 2) When the high degree of positive lung inflation was employed, changes of central venous pressure and heart rate were not significant. 3) The low grade of intrapulmonary pressure increase caused reflex tachycardia in phase 2 and overshooting in phase 4 in response to the systemic arterial blood pressure change. 4) On the other hand, the high degree of intrapulmonary pressure increase caused paradoxical bradycardia in phase 2 and lack of overshooting in phase 4 in response to the systemic arterial blood pressure change. 5) It may be noted that the experimental model employed in the present study is a useful tool to evaluate and analyze the neural and mechanical regulatory factor in response to the graded increase of the positive lung inflation.

급성범발성 복막염의 임상적고찰

윤건호(Kun Ho Yon),오상유(Sang Yoo Oh),김훈남(Hoon Nam Kim),배원길(Won Kil Bae),신현수(Hyun Soo Shin) 대한외과학회 1971 Annals of Surgical Treatment and Research(ASRT) Vol.13 No.9

상부소화관 내시경 시행 후 발생한 흡인성 폐렴 1예

남원욱,장진석,노명환,박정익,김효종,이정석,오상유,유승희 대한상부위장관ㆍ헬리코박터학회 2014 Korean Journal of Helicobacter Upper Gastrointesti Vol.14 No.3

In upper gastrointestinal endoscopy, intravenous anesthesia is commonly used due to its safety and improvement in patient’s satisfaction and comfort. The aim of this approach is to improve the condition for endoscopy. Although the risks of sedation are reported to be very low with sufficient monitoring, we still have few risks that cannot be overlooked including aspiration pneumonia. There have been two reported cases of aspiration pneumonia after endoscopic submucosal dissection. However, to our knowledge, there has been no reports about aspiration pneumonia developing after endoscopy for a young healthy female. So, we report a first case of unexpected aspiration pneumonia after endoscopy.

직장선암으로 오인된 항문 편평상피세포암 1 례

박정익,양웅석,문성원,남원욱,김효종,이정석,오상유 고신대학교(의대) 고신대학교 의과대학 학술지 2014 고신대학교 의과대학 학술지 Vol.29 No.2

We report a case of anal squamous cell carcinoma extended to the rectal mucosa that was clinically mistaken for rectal adenocarcinoma and literature reviwed. Sigmoidoscopic finding showed spherical shaped elevated lesion with central ulceration, interpreted as rectal adenocarcinoma in the distal portion of rectum. Anal squamous cell carcinoma is very rare among gastrointestinal cancer. Pathological study of the biopsy specimen demonstrated squamous cell carcinoma and normal rectal glands. Sigmoidoscopic finding of the presented case showed the ulcerarive lesion contineuosly extended from anal verge upward to the rectum. We postulate that the presented case is primarilly originated from the anal squamous cell carcinoma extended proximally to the rectum. Immunohistochemical stain(p-63) of the biopsy specimens showed squamous cell carcinoma. This patient has been completely recovered after treartment of chemoradiation and trans-anal excision. We present a case of anal squamous cell carcinoma invading rectal mucosa clinically mistaken for rectal adenocarcinoma and literature reviewed.

증례 : 신장 ; 만성 살리실산염 중독에 의한 가성 고염소혈증 및 대사장애 1예

김효종 ( Hyo Jong Kim ),오유정 ( You Jeong Oh ),이정석 ( Jeong Seok Lee ),오상유 ( Sang Yu Oh ),이종윤 ( Jong Yoon Lee ) 대한내과학회 2015 대한내과학회지 Vol.89 No.4

일반적으로 살리실산염 중독 시에는 high anion gap의 대사성 산증이 발현된다. 저자들은 negative anion gap의 대사성 산증과 가성 고염소혈증, 호흡성 알칼리증이 있는 만성살리실산염 중독 환자를 경험하였다. 살리실산염과 같은 약물 중독의 경우 드물게 가성 고염소혈증이 나타날 수도 있기 때문에 적극적인 병력 청취가 필요하고, 보존적 치료를 하면서 투석 시기를 결정하는 것이 중요하다. Despite the increasing use of alternative analgesic agents, salicylate overdose remains a not-uncommon problem. Severe poisoning is life threatening, so prompt treatment and supportive measures are required to reduce mortality. Generally, salicylate intoxication results in the development of high-anion-gap metabolic acidosis. However, outside of Korea, normal-anion-gap or negative-anion-gap metabolic acidosis with hyperchloremia is rarely reported. We report herein the case of an 83-year-old female patient with chronic aspirin intake who presented with hyperchloremia and a negative anion gap. The patient’s symptoms improved with conservative treatment and hemodialysis; notably, her chloride levels decreased as her blood salicylate concentrations decreased. Salicylate may cause hyperchloremia, demonstrating the importance of careful documentation of patient medication histories. (Korean J Med 2015;89:457-460)

臍靜脈을 利用한 門脈壓減壓術

尹健鎬(Kun Ho Youn),李東植(Dong Shik Lee),張敬植(Kyung Shik Chang),吳相由(Sang Yoo Oh),李在運(Gae Un Lee) 대한외과학회 1968 Annals of Surgical Treatment and Research Vol.10 No.9