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혈액응고 제8인자 유전자 내부의 BclI, Xbal RFLP 와 St14 VNTR 에 나타나는 다형인자의 빈도추정과 A 형 혈우병 8가계의 연관 분석
문홍모,김장성,오달균,강신혜 한국유전학회 1993 Genes & Genomics Vol.15 No.4
With the acid of PCR, we used two intragenic factor VIII gene RFLP markers(BclI-intron 18 and XbaI-intron 22) and one extragenic marker(St 14 VNTR) to estimate allele frequencies of these markers from 100 Korean individuals and to analyze eight Korean hemophilia A families. Allele frequencies for the presence of the enzyme sites and heterozygosity expectations were 86 and 24% for BclI-intron 18 RFLP marker, and 62% and 47% for XbaI-intron 22 RFLP marker, respectively. When BclI-intron 18 was homozygous, 31.5% of women was predicted to be heterozygous for XbaI-intron 22. Number of repeats in St14 VNTR loci from Korean males differed from those previously reported for Caucasian males. 68% of Korean women was estimated to be heterozygous for St14 VNTR marker. Analysis of hemophilia A families with BclI-RFLP and St14 VNTR proved to be informative for six out of eight families tested.
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ICR 생쥐의 해마에서 스타니오칼신 2가 세포생성에 미치는 영향
이재원,변종선,이균영,오달균,전완주,김성수,이희제 대한정신약물학회 2010 대한정신약물학회지 Vol.21 No.3
본 연구에서는 STC2가 신경생성에 미치는 영향을 알아보기 위하여 100 nM STC2와 BrdU를 ICR 생쥐(23~25 g)의뇌실 내와 복강에 각각 차례대로 투여하였다. STC2와 BrdU 처치 후 24시간과 3주 후에 각 군의 실험동물을 희생하고그 뇌 절편으로 BrdU와 인산화 CREB의 면역조직화학법을시행하였다. 24시간 경과한 STC2 투여군의 해마 치상회에서 선조세포들의 증식이 과립구하 지역에서 대조군과 비교하여 유의하게 증가하였다. 하지만 STC2 처치 후 3주 후의뇌 조직에서는 신경생성의 차이는 없었다. 한편 해마 치상회에서의 CREB 인산화 변화는 24시간 경과한 STC2 투여군에서 증가하였다. STC2 투여군에서 과립구하 지역에서의선조세포들이 증가했다는 점과 이 영역에서 CREB 인산화가증가했다는 점은 STC2가 CREB 신호전달 체계를 통해 선조세포들의 증식을 증가시킬 가능성을 시사한다. Objective Stanniocalcin 2 (STC2) is a glycoprotein hormone that is widely expressed in mammalian kidney, heart, and thymus. However, the potential function of STC2 in the brain is less understood. In this study, we investigated whether treatment with STC2 influenced cell proliferation and neurogenesis in imprinting control region (ICR) mice. Methods 100 nM STC2 and 5’-bromo-2’-deoxyuridine (BrdU) (50 mg/kg) were administered intracerebroventricularly and intraperitoneally, respectively. On days 1 and 21 after the BrdU injection, sections of STC2-treated group and controls were carried out immunohistochemistry using anti-BrdU and anti-phosphor-cAMP-response element-binding protein (CREB) antibody. Results We found that the number of BrdU-positive cells was significantly increased in the hippocampal dentate gyrus (DG),compared with controls. Next, we observed that CREB phosphorylation was decreased in the hippocampal DG of STC2-treated group versus the controls. Conclusion These results suggest that STC2 treatment may increase cell proliferation by increasing CREB phosphorylation in the subgranular zone of the DG.
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조현주 ( Hyun Joo Cho ),이현경 ( Hyun Kyung Lee ),김동석 ( Dong Seok Kim ),박경찬 ( Kyoung Chan Park ),장민열 ( Min Youl Chang ),김진준 ( Jin Jun Kim ),이천구 ( Cheon Koo Lee ),오달균 ( Dahl Kyun Oh ) 한국조직공학·재생의학회 2007 조직공학과 재생의학 Vol.4 No.3
In this study, the effects of hGH were analyzed by using living skin equivalents(LSEs) models. In monolayer culture of keratinocytes, hGH was not cytotoxic to cells in the range of 0.0005~0.5%. However, hGH inhibit keratinocytes proliferation in all tested concentrations. In fibroblasts, hGH was slightly toxic to fibroblasts at 0.5% concentration. However, hGH stimulate fibroblasts proliferation at the concentrations between 0.005~0.05%. These results showed that hGH is not cytotoxic to epidermal cells up to 0.5% concentration. But, it can be said that different pathways are mediated by hGH in keratinocytes and fibroblasts. Then, the effects of hGH were tested using LSE models. For this study, 0.05% concentration of hGH was used. It is thought that epidermis in LSE is hyperproliferative. Thus, parakeratotic epidermis is observed and granular layer is not well formed. To suppress the hyperproliferative potential of LSEs, high concentration of rh-EGF(10 ng/ml) was usually added in the preparation of LSEs. In this study, 0.05% hGH was added and epidermal thickness of hGH treated model looked slightly thinner than that of rh-EGF models. Interestingly, parakeratosis was not observed in both rh-EGF and hGH models. Thus, it can be said that rh-EGF and hGH can control the hyper-proliferation of keratinocytes in the reconstruction of SEs. In the reconstruction of epidermis, basement membrane formation is also very important. Results showed that α6 integrin and ß1 integrin expression is elevated in hGH models. Furthermore, involucrin expression was evident at upper epidermis. However, expression of PCNA and p63 was not different compared to other models. Results suggest that hGH promote the formation of basement membrane and differentiation.
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