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18S rDNA를 이용한 인삼(Panax ginseng)의 내생균근 균의 동정
엄안흠,정현숙,김동훈,어주경 한국응용생명화학회 2004 Journal of Applied Biological Chemistry (J. Appl. Vol.47 No.2
Morphological observation of roots and molecular technique were used to investigate the symbiotic relationships between arbuscular mycorrhizal (AM) fungi and ginseng roots. Korean ginseng, Panax ginseng, was collected from 8 sites in Korea. Colonization pattern of AM fungi in ginseng roots was determined as an Arum type under light microscopes. Nested PCR using AM fungal specific primers was employed to amplify a partial region on 18s rDNA of AM fungi from the root extracted mixed DNA. The amplified DNA was cloned and analyzed by random fragment length polymorphism (RFLP) with restriction enzymes, AluI, HinfI and AsuC21. One from each RFLP pattern was selected for sequencing. A total 16 clones were sequenced and identified as 2 species of AM fungi; Paraglomus brasilianum and Glomus spurcum. Paramglomus brasilianum was found from most of the ginseng roots, in this syudy suggesting that this species of AM fungi could have specific relationship with the ginseng root. Possible roles of AM fungal species in ginseng roots are discussed.
Diversity of Arbuscular Mycorrhizal Fungi in Arable and Natural Soils in Korea
엄안흠,문성태,이재구 한국생태학회 2004 Journal of Ecology and Environment Vol.27 No.3
The diversity of arbuscular mycorrhizal fungi (AM) was investigated in cultivated and natural fieldsites of Chungbuk, Korea. Soils were collected from rhizosphere of Sorghum bicolor, Fagopyrum esculentum and Glycine max in cultivated sites, and of Miscanthus sinensis, Glycine soja and Lespedeza cuneata in natural sites. of AM fungi were significantly lower in the arable sites. While only AM fungal spores belong to Glomus and Acaulospora were found in arable field sites, more diverse fungal spores including Gigasporaceae were found in natural grasslands. AM fungal spore composition did not significantly difer among crop plant species. Results suggest that the agricultural practices were significantly influenced on AM fungal comunity structures and mycorrhizal developments.
속리산 국립공원의 경관 및 식생 분석과 그 보존을 위한 생태학적 제안
엄안흠,조용찬,신현철,이창석 한국생태학회 2004 Journal of Ecology and Environment Vol.27 No.3
Vegetation established through the natural process, such as Quercus mongolica, Pinus densiflora,Q. variabilis, Q. acutissima, Carpinus laxiflora, Q. aliena and Q. serrata communities and artificially introduced vegetation, which are composed of Larix leptolepis and P. rigida plantations, are established in the mountainous land of the Mt. Songnisan National Park. On the other hand, the developed lands, which are consisted of agricultural fields, residential areas, commercial areas related to tourism, etc. appear in the lowland around streams. Based on the spatial distribution of vegetation, the southern district, which is attributed to Naesongni-myun by administrative system, showed higher natural degree and vegetation diversity. However, most of the other districts, which are attributed to Cheongcheon- and Chilseong-myuns, are covered with the Korean red pine forest, a product of artificial influence, and plantation also occupied higher percentage. Thereby both vegetation diversity and natural degree are lowering. A result of ordination by DCA showed that sands tended to be arranged by depending on the topographic condition. Species diversity of plant communities was higher in broad-leaved stands rather than in coniferous ones and in stands of the early stage than in ones of the late stage. The result of analysis on vegetation dynamics implied that vegetation of this region would be dominated by Carpinus laxiflora, Quercus mongolica and Pinus densiflora communities in the future. However, considered occurrence the of disturbance and response of vegetation on that, this estimation may different somewhat from an actual situation. Conservation strategies of the Mt. Songnisan National Park were discussed in viewpoints of landscape ecology, and conservation of major plant communities and biodiversity. 속리산 국립공원의 산지에는 신갈나무군락, 소나무군락, 굴참나무군락, 상수리나무군락, 서어나무군락, 갈참나무군락 및 졸참나무군락의 자연적 과정을 통해 성립된 식생과 일본잎갈나무 조림지와 리기다소나무 조림지로 이루어진 인위적으로 조성된 식생이 성립하고 있다. 한편, 하천 주변의 저지대에는 농경지, 주거 지, 상업시설 등으로 이루어진 개발지가 분포하고 있다. 식생의 공간적 분포에 의하면, 행정구역상 충북 보은군 내속리면과 경북 상주시 화북면에 속하는 공원구역 남부 약 1/3 지역은 자연성과 식생의 다양성이 높았지만 청천면 및 칠성면에 속하는 나머지 지역은 그 대부분을 소나무림이 차지하고 조림지가 차지하는 비율도 높아 식생의 다양성과 자연성이 모두 낮았다. 조사된 식분을 서열법으로 처리한 결과, 식분의 배열은 고도,사면 등의 지형요인과 그것에서 비롯된 수분요인에 의해 지배되는 경향이었다. 주요 목본식물의 직경 급별 빈도분포를 분석하여 식생의 동태를 분석한 결과, 향후 이 지역의 식생은 서어나무군락, 신갈나무군락 및 소 나무군락이 중심을 이루는 식생으로 재편될 전망이다. 그러나 교란의 발생과 그것에 대한 식생의 반응을 고려 하면, 이러한 판단은 실제와 다소 차이를 보일 가능성도 있다. 이상의 결과를 종합한 속리산 국립공원의 보전전략이 경관생태, 주요 식물군락의 보전 및 생물다양성 보전의 관점에서 검토되었다.
심미영,엄안흠 한국생태학회 2009 Journal of Ecology and Environment Vol.32 No.1
This study was conducted to investigate ectomycorrhizal (ECM) diversity on Pinus densiflora seedlings in a disturbed pine forest. Pine seedlings less than one year old were collected from disturbed and undisturbed sites in the Mt. Songni region. The belowground ECM fungal communities colonizing P. densiflora seedlings were studied using morphotyping and DNA sequencing. The relative abundance of ECM root tips was significantly higher in the undisturbed sites than in the disturbed sites, and the ECM species diversity was lower in the disturbed sites than in the undisturbed sites. In addition, the ECM community composition was significantly different in the disturbed and undisturbed forest sites. This study was conducted to investigate ectomycorrhizal (ECM) diversity on Pinus densiflora seedlings in a disturbed pine forest. Pine seedlings less than one year old were collected from disturbed and undisturbed sites in the Mt. Songni region. The belowground ECM fungal communities colonizing P. densiflora seedlings were studied using morphotyping and DNA sequencing. The relative abundance of ECM root tips was significantly higher in the undisturbed sites than in the disturbed sites, and the ECM species diversity was lower in the disturbed sites than in the undisturbed sites. In addition, the ECM community composition was significantly different in the disturbed and undisturbed forest sites.