Genetic profiling of thoroughbred racehorses by microsatellite marker analysis

어정우,김희수,김정안,최봉환,박경도,양영목,이학교,도경탁,조병욱 한국유전학회 2014 Genes & Genomics Vol.36 No.1

The thoroughbred (TB) horse breed has exceptionalphysiological traits and is well-known in horse racing. It has been bred for endurance, strength, and speed for300 years. Recently, there have been many studies on themolecular characteristics or mechanisms associated withelite athletic performance of the TB breed, and sequencevariants in exercise-related genes have also been reported. Here, using 12 microsatellite markers, we assessed geneticdiversity and relationships among two groups of TB racehorses(registered by the Seoul Race Park of the KoreaRacing Authority) that showed the 48 highest and lowestscores in races. When comparing allelic variation between the highest- and lowest-scoring TBhorse groups, a total of 53different alleles were detected by genotyping with 60FAMlabelledprimers. The genetic variation between the twogroups was similar [mean number of alleles (NA) = 4.00 and4.08; allelic richness (AR) = 3.95 and 4.01; observed heterozygosity(HO) = 0.53 and 0.56, respectively]. However,among the 53 alleles that we assessed, four alleles weredetected only in the highest-scoring and five alleles only inthe lowest-scoring racing horse group. The group-specificunique alleles indicate that the microsatellite markers coulddistinguish horses that showed high or low scores in a race. This study demonstrates the feasibility of microsatelliteanalyses for selection of superior racehorses and suggestsfurther study to understand the relationship betweenmolecular features and phenotype (racing performance).

Polymorphism analysis of tyrosine hydroxylase (TH) in military working dogs

어정우,최봉환,정이든,권윤정,김태훈,성환후,김희수 한국유전학회 2013 Genes & Genomics Vol.35 No.6

Canine and human behavior are shaped by similar evolutionary processes, yet the identification of the behavioral phenotype is often difficult. A widely used method relies on breed stereotypes provided by experts such as dog trainers. To reveal a valid association between behavior and genetic factors, an association study of behavioral phenotyping and genotyping is essential. We screened for variable number of tandem repeat (VNTR)polymorphisms in intron 4 of the tyrosine hydroxylase gene in military working dogs (belonging to pass and fail groups based on the results of an in-training examination conducted by the drillmaster), which were scored based on possessiveness, audaciousness, concentration, and motor ability by qualification examination. We first characterized each genotype by sequencing, in which the 1/1 type consists of a single copy of a 36-bp sequence and the 2/2 type is a duplicated form of the 36-bp repeat unit. The 1/2alleles showed a single nucleotide change as a heteroduplex,which generated a PCR product of similar size as that of the 1/1-182-bp. The military working dogs showed the 2/2 type of VNTR and heteroduplex. For the pass group,two dogs possessed 2/2 type (40 %), whereas three dogs were of the heteroduplex type (60 %). However, all members of fail group showed the 2/2 type (100 %). These data indicate that repeat polymorphisms with behavioral phenotyping can identify military working dogs that would pass or fail the in-training examination.

Biological changes of transposable elements by radiation: recent progress

배진한,어정우,김태오,이주미 한국유전학회 2015 Genes & Genomics Vol.37 No.2

Mobile genetic elements within genomes havebeen known to drive genome evolution in diverse ways. Since developing high-technology for whole-genomesequencing, many researchers have focused on not onlyhow mobile genetic elements have affected the evolution ofgenes and their function, particularly of human and mammals,but also how these elements involve in gene activationor inactivation associated with a number of humandiseases. Here we describe what kind of biological changesby mobile genetic elements contribute to disease, especiallycancer and present an overview of what is knownabout this large, and largely unexplored, segment of thegenome. Finally, we discuss understanding the genomicchanges or cellular responses to genotoxic stress such asradiation may permit to implicate for potential clinicalapplication.

Epigenetic Changes of LDHA and GYS1 Genes after Exercise in Thoroughbred Horses

김정안,셀밤,어정우,권윤정,최유리,김희수 한국실험동물학회 2014 한국실험동물학회 학술발표대회 논문집 Vol.2014 No.8

Structure and Expression Analyses of SVA Elements in Relation to Functional Genes

권윤정,최유리,어정우,노유나,김정안,정이든,이자랑,김희수 한국유전체학회 2013 Genomics & informatics Vol.11 No.3

SINE-VNTR-Alu (SVA) elements are present in hominoid primates and are divided into 6 subfamilies (SVA-A to SVA-F) and active in the human population. Using a bioinformatic tool, 22 SVA element-associated genes are identified in the human genome. In an analysis of genomic structure, SVA elements are detected in the 5′ untranslated region (UTR) of HGSNAT (SVA-B), MRGPRX3 (SVA-D), HYAL1 (SVA-F), TCHH (SVA-F), and ATXN2L (SVA-F) genes, while some elements are observed in the 3′UTR of SPICE1 (SVA-B), TDRKH (SVA-C), GOSR1 (SVA-D), BBS5 (SVA-D), NEK5 (SVA-D), ABHD2 (SVA-F), C1QTNF7 (SVA-F),ORC6L (SVA-F), TMEM69 (SVA-F), and CCDC137 (SVA-F) genes. They could contribute to exon extension or supplying poly A signals. LEPR (SVA-C), ALOX5 (SVA-D), PDS5B (SVA-D), and ABCA10 (SVA-F) genes also showed alternative transcripts by SVA exonization events. Dominant expression of HYAL1_SVA appeared in lung tissues, while HYAL1_noSVA showed ubiquitous expression in various human tissues. Expression of both transcripts (TDRKH_SVA and TDRKH_noSVA) of the TDRKH gene appeared to be ubiquitous. Taken together, these data suggest that SVA elements cause transcript isoforms that contribute to modulation of gene regulation in various human tissues.

Differently gene expression associated with methylation in canine genome

권윤정,김희수,어정우,최유리,이희은,정호임,김정안 한국실험동물학회 2014 한국실험동물학회 학술발표대회 논문집 Vol.2014 No.8

Hepatitis A Virus Infection from a Contaminated Tap of Ground Water Facility in a Neighborhood Park, Republic of Korea

유석현,원설아,어정우,송준영 대한감염학회 2019 Infection and Chemotherapy Vol.51 No.1

A patient with Hepatitis A virus (HAV) infection was notified to the public health authority. The identical HAV was found at a water dispensing outlet at the ground water facility and the toilet close to the facility. Serosurveillance in the community was conducted. Suspicious individuals were asked to visit city public health center and had a serologic test for anti-HAV Ig M. Overall, 100 individuals were tested, and all were negative for the anti-HAV Ig M. In our study cohort, we could not identify additional case of HAV infection.

Statistical analysis and genetic diversity of three dog breeds using simple sequence repeats

정호임,김희수,최봉환,어정우,권윤정,이희은,최유리,김정안,김태훈,성환후,이동훈,하지홍,한국일 한국유전학회 2014 Genes & Genomics Vol.36 No.6

There are more than 400 pure dog breedsdeveloped through intentional artificial selection andpurebred breeding. Purebred animals have higher risk ofinbreeding depression and hereditary diseases. We investigatedthe genetic diversity and structure of three dogbreeds in South Korea by using 12 microsatellite loci forone Korean native dog breed, Sapsaree, and two foreignbreeds, German shepherd and Belgian Malinois. The meanallele number of nine loci across all dog breeds was 4.833,and the number of alleles per locus ranged from 2 to 8. Themean of expected and observed heterozygosity were 0.415and 0.577, respectively. Sapsaree, Korean native dog, hadhigher level of genetic diversity than the foreign Germanshepherd and Belgian Malinois. The highest mean value ofpolymorphism information content was found in Sapsaree(0.480), followed by Belgian Malinois (0.373) and Germanshepherd (0.355). Pairwise genetic differentiation wasestimated using fixation index FST. Sapsaree and Germanshepherd (FST = 0.2536) and Sapsaree and Belgian Malinois(FST = 0.2522) had very great genetic differentiation,while moderate level of genetic differentiation wasobserved between German shepherd and Belgian Malinois(FST = 0.1003). These genetic information and structure ofthe three dog breeds will be effective in conservation andpreservation of the genetic diversity of the three dogbreeds.

특수 목적견으로서의 품성 및 능력 관련 유전자들에 관한 생물정보학적 분석

권윤정(Yun-Jeong Kwon),어정우(Jungwoo Eo),최봉환(Bong-Hwan Choi),최유리(Yuri Choi),김정안(Jeong-An Gim),김다희(Dahee Kim),김태헌(Tae-Hun Kim),성환후(Hwan-Hoo Seong),김희수(Heui-Soo Kim) 한국생명과학회 2013 생명과학회지 Vol.23 No.11

특수 목적견(구조견, 군견, 안내견 및 탐지견)은 집중력, 소유욕, 대담성 등을 기반으로 한 훈련시험을 통해 선별된다. 최근 특수견으로서의 특수한 능력 및 품성에 대해 유전적인 정보가 중요한 인자로 다뤄지고 있다. 본 연구에서는 특수견으로서의 개의 특수한 능력 및 품성과 관련된 유전자들의 분자적인 특징을 고찰하고자 하였다. 이전 연구에서 보고된 24개의 유전자(AR, BDNF, DAT, DBH, DGCR2, DRD4, MAOA, MAOB, SLC6A4, TH, TPH2, IFT88, KCNA3, TBR2, TRKB, ACE, GNB1, MSTN, PLCL1, SLC25A22, WFIKKN2, APOE, GRIN2B, PIK3CG)를 선택하여 품성, 후각, 운동 및 학습능력 관련 유전자, 네 가지 카테고리로 분류하였다. 본 연구에서는 생물학적인 기법을 이용하여 이 유전자들의 염색체상의 위치, 유전자들 간의 네트워크를 통한 상호관계를 조사하였으며, 어떤 생물학적 기능과 관련이 있는지 Gene Ontology 분석과 데이터베이스를 기반으로 in silico 발현 양상을 살펴보았다. 또한 이전 연구를 통하여 품성 관련 유전자들의 다양한 유전적 다형성에 대한 보고를 조사하였다. 본 연구는 특수견으로서 주요하게 고려되는 개의 고유한 능력 및 품성 관련된 유전자에 대해 분자적 특징을 제시하고 있다. 이 후보 유전자들은 개의 특수한 표현형과의 관계를 밝힐 수 있는 연구의 기초자료로서 이용될 수 있을 뿐만 아니라 핵심적인 유전인자로 응용되어 신속하고 정확한 특수견 선발에 기여할 수 있을 것으로 전망된다. Working dogs, such as rescue dogs, military watch dogs, guide dogs, and search dogs, are selected by in-training examination of desired traits, including concentration, possessiveness, and boldness. In recent years, genetic information has been considered to be an important factor for the outstanding abilities of working dogs. To characterize the molecular features of the canine genes related to phenotypes for working dogs, we investigated the 24 previously reported genes (AR, BDNF, DAT, DBH, DGCR2, DRD4, MAOA, MAOB, SLC6A4, TH, TPH2, IFT88, KCNA3, TBR2, TRKB, ACE, GNB1, MSTN, PLCL1, SLC25A22, WFIKKN2, APOE, GRIN2B, and PIK3CG) that were categorized to personality, olfactory sense, and athletic/learning ability. We analyzed the chromosomal location, gene-gene interactions, Gene Ontology, and expression patterns of these genes using bioinformatic tools. In addition, variable numbers of tandem repeat (VNTR) or microsatellite (MS) polymorphism in the AR, MAOA, MAOB, TH, DAT, DBH, and DRD4 genes were reviewed. Taken together, we suggest that the genetic background of the canine genes associated with various working dog behaviors and skill performance attributes could be used for proper selection of superior working dogs.

The Change of Global DNA Methylation patterns before and after Exercise in the Thoroughbred Horse via MeDIP-Seq

김정안,홍창표,최유리,어정우,권윤정,이자랑,정이든,배진한,셀밤,박종화,김희수 한국실험동물학회 2014 한국실험동물학회 학술발표대회 논문집 Vol.2014 No.8