식육중의 잔류 항생.항균제의 검정에 관한 연구 (II) - Penicillin G , Chloramphenicol, Thiamphenicol, Gas chromatography/Mass spectrometry 동시 분석 -

류재천,양종순,서지원,김명수,박종세 한국식품위생안전성학회 1993 한국식품위생안전성학회지 Vol.8 No.1

어패류를 포함한 식용 육류중에 잔류하는 항생물질 및 항균성물질을 검출하기 위하여, 분석기기로서 Gas chromatography/ Mass spectrometry(GC/MS)를 사용한 동시분석법을 개발하였다. 여러 항생.항균제중에서 penicillin G, chloramphenicol, thiamphenicol을 중심으로 간단한 전처리과정과 유도체화를 하여 GC/MS 분석을 시행하였다. 전처리 과정을 요약하면 pH 4.0의 0.01 M EDTA-2Na McIlvaine buffer로의 추출, n-hexane으로의 탈지, Bond-Elute $C_{18}$ cartridge에 흡착된 물질의 0.01 M-methnolic oxalic acid로의 용출 그리고 건조 후 유도체화의 순으로 되어 있다. 본 방법에 의한 1 ppm spike시의 회수율은 penicillin G, chloramphenicol, thiamphenicol 각각 63.5%, 76.3%, 84.7%이었고, 검출한계는 각각 시료 g당 0.6, 0.085, $0.084\;\mu\textrm{g}$이었다. 또한 GC/MS 확인과정에서 실제 잔류농도가 1 ppm 이상이면 full scan spectrum으로 확인이 가능하였다. 이와 같은 GC/MS에 의한 잔류물질의 정량 및 확인시험은 앞의 미생물학적 방법의 복원은 물론 식품의 안전성 재고 및 규제독성의 측면에서도 매우 뜻 있는 연구라 하겠다. In an attempt to quantitate and qualitate residual antibiotics and antibacterial agents in meat simultaneously, we studied a gas chromatography-mass spectrometry(GC/M8) analysis. For a simultaneous analysis of penicillin G, chloramphenicol and thiamphenicol in meat, a simple and rapid clean-up procedure including extraction with 0.01 M EDTA-2Na Mcilvaine buffer (pH 4.0), defatting with n-hexane, and elution with 0.01M-methanolic oxalic acid from Bond Elute $C_{18}$ cartridge, and quantitation by selected ion monitoring (SIM) mode after derivatization was performed. The recoveries (%) of penicillin G, chloramphenicol and thiamphenicol (CV, %) at 1 ppm fortification level were 63.5 (7.6), 76.3 (8.1) and 84.7 (2.0), and the detection limits of those were 0.6, 0.085 and $0.084\;\mu\textrm{g}$ beef, respectively. This method using 81M mode allows excellent detection and quantitation of residual antibiotics and antibacterial agents in meat. Moreover, confirmation by a full scan electron impact mass spectrum is possible if residual level in the sample in above 1 ppm.

식육중의 잔류 황생 · 홍란제의 검정에 관한 연구(III) Macrolide계 항생물질인 Erythromycin과 Tylosin의 Gas Chromatography /Mass Spectrometry 동시분석

류재천,송윤선,양종순,서자원,김명수,박종세 한국식품위생안전성학회 1993 한국식품위생안전성학회지 Vol.8 No.1

식육중의 잔류 항생.항균제의 검정에 관한 연구(III) Macrolide계 항생물질인 Erythromycin과 Tylosin의 Gas Chromatography/Mass Spectrometry 동시분석

류재천,송윤선,양종순,서지원,김명수,박종세 한국식품위생안전성학회 1993 한국식품위생안전성학회지 Vol.8 No.1

In an attempt to quantitate and qualitate residual antibiotics and antibacterial agents n meat simultaneously, we studied a gas chromatogrphy-mass spectrometry (GC/MS) analysis. For a simultaneous analysis of macrolide antibiotics such as erythromycin and tylosin in meat, the homogenization with MeOH, defatting with n-hexane, extraction with CHCl3, elution with CHCl3 : MeOH=2:1 from Sep-Pak silica cartridge, acid gydrolysis, back extraction with CHCl3, and quantitation by selected ion monitoring(SIM) mode after trimethylsilyl derivatization were performed. The recoveries of erythromycin and tylosin (CV,%) at 10 ppm fortification level were 90.59(4.89) and 45.91(0.20) , and the detection limits of those were 0.02 and 2.0 $\mu\textrm{g}$/g beef, respectively. From these results, the developed analytical method using GC/MS-SIM mode allows excellent detection and quantitation of residual macrolide antibiotics in meats, using complementary method with bio-assay.

비만치료식 개발을 위한 기초연구 : 1 . 식물섬유로서의 알긴산의 비만억제효과 1 . The Inhibitory Effect of Alginic Acid as a Dietary Fiber on Obesity

최진호,최재수,김재연,변대석,임채환,양종순 한국수산학회 1986 한국수산과학회지 Vol.19 No.4

우리나라 長壽者의 生活 및 意識調査에 관한 硏究 - Ⅳ. 長壽者의 過去 家族狀況과 夫婦生活

최진호(Jin-Ho Choi),변재형(Jae-Hyeung Pyeun),임채환(Chae-Hwan Rhim),양종순(Jong-Soon Yang),김수현(Soo-Hyun Kim),김정한(Jeung-Han Kim),이병호(Byeong-Ho Lee),우순임(Soon-Im Woo),최선남(Sun-Nam Choe),변대석(Dae-Seok Byun),김무남(Mu-Nam 한국식품영양과학회 1986 한국식품영양과학회지 Vol.15 No.4

우리나라 長壽者의 生活 및 意識調査硏究의 一環으로, 우리나라 長壽者의 過去 家族狀況과 夫婦生活에대해서 調査ㆍ比較한 結果는 다음과 같다.<br/> 1. 長壽者의 兩親의 死亡年齡을 比較하여 보면 『兩親이 70세 이상』이 전체의 20.3%이고, 『片親이 70세 이상』인 경우가 28.2%로서, 兩親이나 片親中에서 70세이상 長壽한 경우가 전체의 48.5%나 차지하고 있었다. 따라서 壽命과 遺傳的 要因사이에는 깊은 相關이 있음을 나타냈다.<br/> 2. 長壽者의 出生順位를 比較하여 보면 『첫번째』가 41.2%로 가장 많고, 그 다음이 『두번째』(25.1%), 『세번째』(13.7%)의 順으로 감소하고 있었다. 따라서 出生順位가 빠르면 빠를수록 長壽하는 傾向으로 나타냈다.<br/> 3. 長壽者의 兄弟姉妹의 數는 3~5명이 전체 장수자의 57.6%나 차지하고 있었으며, 子女의 數는 3~6명이 전체 장수자의 69.9%나 차지하고 있었다.<br/> 4. 長壽者의 結婚回數는 『1回』가 90.0%로 가장 많고, 그 다음이 『2回』(8.7%)로서, 全體 長壽者의 98.7%가 1回 또는 2回였으며, 長壽者의 結婚生活을 比較하여 보면, 『원만했다』가 전체의 87.6%로 대부분을 차지하고 있어서, 長壽者의 夫婦生活이 대단히 원만했던 것으로 나타났다. The present study was conducted as a part of the research on the investigation on daily life and consciousness of the longevous people in Korea. Surveys were carried out on their familial organization and the marriage life of longevous people in order to achieve the purpose of the research. The materials for 121 males and 258 females aged more than 80 were collected and analyzed from June to November 1985.<br/> The results are summarized as follows:<br/> 1. As for parents' mortality age of longevous people, 48.5% of them showed “both above 70”(20.3%) and/or “either father or mother above 70” (28.2%). Consequently, a significant difference was found between the life span and inherent factor.<br/> 2. By the birth order of longevous people, “1st” had the highest figure of 41.2%, and decreased in the order of “2nd”(25.1%) and “3rd”(l3.7%). It was observed that the more the birth order of longevous people preceded, the more life span of the aged increased.<br/> 3. Three to five persons in the number of brothers and sisters comprised 57.6% of total longevous people surveyed, while 63.9% of them were revealed to have three to six in the number of offsprings.

우리나라 長壽者의 生活 및 意識調査에 관한 硏究 (1) 長壽地域의 地域的 特性

최진호(Jin-Ho Choi),변재형(Jae-Hyeung Pyeun),임채환(Chae-Hwan Rhim),양종순(Jong-Soon Yang),김수현(Soo-Hyun Kim),김정한(Jeung-Han Kim),이병호(Byeong-Ho Lee),우순임(Soon-Im Woo),최선남(Sun-Nam Choe),변대석(Dae-Seok Byun) 군산대학교 수산과학연구소 1994 군산대학교 수산과학연구소 연구논문집 Vol.1 No.-

This study was designed to be a link in the chain of the investigation on daily life and consciousness of longevous people in Korea, and to investigate the regional feature of longevity areas. The daily life and consciousness were investigated on 379 subjects (male 121, female 258) of the aged who were above80 years of age from June to November in 1985. This paper is to report the results investigated the longevity rate, distribution, classification and weather of longevity districts, and also the actual conditions such as the functions of daily life and educational degree of Iongevous people. 1. The number of Iongevous people in Korea was 171.449(male 42.842, female 128,607),and the average longevity rate was 0.46% against total population in Korea(male 0.23%, female 0.69%) 2. Of the longevity rates of shi and/or do in Korea. Cheju (1. 03%) was the highest among those districts and decreased in the order of Chonnam(O.79%). Chonbuk(O.66%), Kyongbuk(O.65%) and Kyongnam(0.61%), whereas the large cities such as Inchon(0.22%), Seoul(0.23%), Pusan(O.23%) and Taegu(0.28%) were remarkably lower than districts in seasides and mountains. 3. The districts above 1.0% of longevity rate in Korea showed 17-guns, and the distribution of these districts was 10-guns of Chonnam, 2-guns of Kvongbuk and Korea, and 1-gun of Kyonggi, Chonbuk and Cheju, respectively. 4. Of these districts, pukcheju(l.65%) was the highest,and decreased in the order of Namhae(1.56%), Sungju(1.24%), Posong(1.22%) and Koksong(1.20%). The highest figure(male 0.71%, female 2.51%) was observed in Pukcheju as contrasted with 0.23%(male) and 0.69%(female) of the average longevity rate in Korea.

우리나라 長壽者의 生活 및 意識調査에 관한 硏究 - Ⅴ. 過去의 健康疾病 및 苦痛에 대한 態度 -

최진호(Jin-Ho Choi),변재형(Jae-Hyeung Pyeun),임채환(Chae-Hwan Rhim),양종순(Jong-Soon Yang),김수현(Soo-Hyun Kim),김정한(Jeung-Han Kim),이병호(Byeong-Ho Lee),우순임(Soon-Im Woo),최선남(Sun-Nam Choe),변대석(Dae-Seok Byun),김무남(Mu-Nam 군산대학교 수산과학연구소 1994 군산대학교 수산과학연구소 연구논문집 Vol.1 No.-

The present study was designed to be link a part of the research on the investigation on daily life and consciousness of the longevous people in Korea, and to investigate the health conditions, and the attitude on disease and suffering of longevous people in the past. 1. By the health conditions of longevous people between 40 and 60 years, ""very good"" showed the highest figure of 71.0%, followed by ""ordinary"" (21.6%). Consequently it is found that 92.6% of total longevous people surveyed maintained the good health conditions above ordinary. 2. Of the experiences of disease and/or injury after 60 years of longevous people, ""never"" showed the highest figure of 74.7%, while ""had"" showed the only 22.6% of total longevous people surveyed. 3. Of the details of disease and/or injury after 60 years of longevous people. ""digestive disease"" showed the highest figure of 32.6%. followed by ""apoplexia cerebri & hypertensions""(14.0%) and ""heart disease""(14.0%), and followed by ""respiratory disease""(12.3%). 4. Of the experiences of suffering between 40 and 60 years of longevous people, ""never"" showed 53.7% of total longevous people surveyed, while the frequency of suffering decreased in the order of ""once""(22.7%), ""2 times""(15.8%) and ""3 times""(4.7%). By the details of suffering between 40 and 60 years of longevous people. ""family life"" showed the highest figure of 85.1%, followed by ""business""(10.1%). 5. By the attitude on suffering of longevous people, ""tried to forget soon"" showed the highest figure of 54.2%, followed by ""continuous worried""(23.3%), and followed by ""not worried""(7.2%).

우리나라 長壽者의 生活 및 意識調査에 관한 硏究 Ⅳ. 長壽者의 過去 家族狀況과 夫婦生活

최진호(Jin-Ho Choi),변재형(Jae-Hyeung Pyeun),임채환(Chae-Hwan Rhim),양종순(Jong-Soon Yang),김수현(Soo-Hyun Kim),김정한(Jeung-Han Kim),이병호(Byeong-Ho Lee),우순임(Soon-Im Woo),최선남(Sun-Nam Choe),변대석(Dae-Seok Byun),김무남(Mu-Nam 군산대학교 수산과학연구소 1994 군산대학교 수산과학연구소 연구논문집 Vol.1 No.-

The present study was conducted as a part of the research on the investigation on daily life and consciousness of the longevous people in Korea. Surveys were carried out on their familial organization and the marriage life of longevous people in order to achieve the purpose of the regearch. The materials for 121 males and 258 females aged more than 80 were collected and analyzed from June to November 1985. The results are summarized as follows: 1. As for parents' mortality age of longevous people, 48.5% of them showed "both above 70%"(20.3%) and/or "either father or mother above 70"(28.2%). Consequently, a significant difference was found between the life span and inherent factor. 2. By the birth order of longevous people, "1st" had the highest figure of 41.2%. and decreased in the order of "2nd"(25.1%) and "3rd"(l3.7%). It was observed that the more the birth order of longevous people preceded, the more life span of the aged increased. 3. Three to five persons in the number of brothers and sisters comprised 57.6% of total longevous people surveyed, while 63.9% of them were revealed to have three to six in the number of offsprings.

우리나라 長壽者의 生活 및 意識調査에 관한 硏究 Ⅲ. 長壽者의 過去 食生活 特性

최진호(Jin-Ho Choi),변재형(Jae-Hyeung Pyeun),임채환(Chae-Hwan Rhim),양종순(Jong-Soon Yang),김수현(Soo-Hyun Kim),김정한(Jeung-Han Kim),이병호(Byeong-Ho Lee),우순임(Soon-Im Woo),최선남(Sun-Nam Choe),변대석(Dae-Seok Byun),김무남(Mu-Nam 군산대학교 수산과학연구소 1994 군산대학교 수산과학연구소 연구논문집 Vol.1 No.-

This study was designed to be link in the chain of the investigation on dally life and consciousness of longevol.ls people in Korea. and to jnvestigate the dietary life and its habit of longevous people in the past. The dietary Hie and its habit were surveyed on 319 subjects (male 121. female 258) of the aged who were above 80 years of age, from June to November in 1985. 1. Of the taste on staple food of longevous peoples in the past(between 40 and 60 years). ""meal mixed with barley"" had the highest figure of 49.9%, followed by ""boiled rice""(47.0%). But it is showed that male preferred ""meal mixed with barley""(56.2%) to ""boiled rice""(41.3%), whereas female slightly preferred ""boiled rice""(49.6%) to ""meal mixed with barley""(46.9%). 2. Of the experience of the food taste of these aged in the past, ""not salted"" and ""light"" had the highest figures of all 40.6%. It is showed that longevous peoples preferred ""not salted""(40.6%) and ""light""(40.6%) to ""saIty""(24.5%) and ""oily""(9.8%), respectively. 3. By comparison of the intake state of animal protein(meat and fish) of these aged in the past, ""occasionally"" was the highest of 55.4%, and decreased in the order of ""almost daily"" (31.9%) and ""scarcely""(I2.7%). Therefore, it is believed that 87.5% of longevous peoples has taken animal protein ""occasionally"" and ""almost daily"". 4. The intakes of major foods above 4~6 times a week of these aged in the past and present showed 50.4% and 67.5% for meat, fish, egg and soybean products, 26.6% and 51.5% for milk and its products, 89.9% and 81.0% for vegetables, 58.9% and 64.0% for oiled food, and 57.0% and 62.9% for seaweeds, respectively.

우리나라 장수자의 생활 및 의식조사에 관한 연구

최진호,김수현,이병호,최선남,우순임,변대석,김정한,임채환,양종순,편재형 한국식생활문화학회 1986 韓國食生活文化學會誌 Vol.1 No.3

우리나라 長壽者의 生活 및 意識調査 硏究의 一環으로, 우리나라 長壽者의 過去의 社會生活과 日常生活習慣에 대해서 分析·比較한 結果는 다음과 같다. 1. 長壽者의 40∼60세경의 起床 및 就寢時間이 『대체로 일정했다』고 대답한 사람이 전체의 81.8% 및 63.1%였으며, 장수자의 59.4%가 6시전에 기상했으며, 또 61.3%가 오후 9시∼11시 사이에 취침하고 있었다. 그러나 男女別 差異는 발견할 수 없었다. 2. 長壽者의 最長職業중에서 農業, 漁業, 家事從事 등 自營業이 전체의 86.3%로 가장 많았으나, 經營管理職(0.8%), 小企業經營(0.8%), 公務員 및 會社員(1.8%) 등은 아주 적었다. 따라서 長壽者의 勞動程度도 肉體的 勞動이 95.7%로 대부분을 차지하고 있었으며 반면 精神的 勞動은 2.8%에 지나지 않았다. 이러한 사실은 이들 長壽者의 敎育程度와 밀접한 關係가 있을 것으로 생각되었다. 3. 長壽者가 職場이나 家事에서 은퇴한 年齡은 전체의 58.3%가 70세 이상이었으며, 그들 중의 24.3%는 80세 이상에서 은퇴한 것으로 나타났다. 4. 長壽者의 57.2%가 職場이나 家事에서 은퇴한 후 가정에서 어떤 役割을 분담하고 있었던 반면, 그들 중의 35.9%는 아무 일도 하지 않고 있었다. 또 가정에서 役割을 분담하지 않은 경우 消日하는 방법은 『집에 그냥 있었다』(50%)가 가장 많고, 그 다음은 『老人亭에 갔다』(30.9%) 『침대에 누워 있었다』(10.3%), 『養老院에 갔다』(8.8%)의 순이었다. 5. 長壽者가 職場이나 家事에서 은퇴하기 전의 交際狀態를 비교하여 보면 交際가 『빈번했다』가 38.5%, 『보통이다』가 38.0%, 『거의 없었다』가 19.0%로 나타났으며, 또 職場이나 家事에서 은퇴한 후에도 계속 交際狀態를 유지한 경우도 56.5%나 차지하고 있었다. 6. 長壽者가 職場이나 家事에서 은퇴한 후 趣味나 娛樂生活을 한 사람은 38.5%로 낮은 반면 趣味나 娛樂을 갖고 있지 않은 사람은 53.0%나 되었다. 이들 長壽者의 은퇴 후 趣味 및 娛樂活動의 內容을 보면 TV 視聽(79.2%), 孫子와의 對話(54.2%), 집안에서 잔손질(35.4%), 信仰生活(25.0%)의 순이다. This study was designed to be link in the chain of the investigation on daily life and consciousness of longevous people in Korea, and to investigate the social life and daily life habit in the past of longevous people. The social life and daily life habit were surveyed on 379 subjects (male 121, female 258) of the aged who were above 80 years of age, from June to November in 1985. 1. "Generally regular" in the rising time and bedtime between 40 and 60 years of longevous people had the highest figures of 81.8% and 63.1%, respectively. The longevous peoples of 59.4% got up by before 6 a.m., and 61.3% of these aged went to bed from 9 to 11 p.m. But there was no significant between male and female. 2. Of the occupation engaged in the longest term of longevous people, the self-management(86.3%) included farming, fishing and household affairs was the highest, whereas administrative position, small-scale management and white-collar worker were only 0.8%, 0.8% and 1.8%, respectively. Therefore, their work involved mainly physical labor (heavy 48.5%, light 47.2%), and mental work was only 2.8% of longevous people. It is believed that this fact was deeply related to the educational degree of them. 3. The longevous people of 58.3% retired from the occupation and household affairs above 70 years of age, and especially 24.3% of them is including the aged above 80 years of age. 4. The longevous people of 57.2% were shared the role in the home after retirement from the occupation and household affairs, whereas 35.9% of them didn't share. Of the way in killing time in case having no role in the home, "at home"(50.0%) was the highest, and decreased in the order of "old people's home" (30.9%), "ill in bed" (10.3%) and "asylum for the aged" (8.8%). 5. The acquaintance frequency before retirement from the occupation and household affairs of longevous people showed in the order of "wide" (38.5%), "common" (38.0%) and "narrow" (19.0%), and 56.5% of them had the acquaintance after retirement. 6. Only the longevous people of 38.5% had the hobby and recreation activities of "more than once" after retirement from the occupation and household affairs, but 53.0% of them was none. Of the kinds of hobby and/or recreation, "TV-watching" (79.2%) was the highest, and decreased in the order of "talking with grandchild" (54.2 %), "working at home" (35.4%), "religion" (25.0%) and "chess etc." (18.1%).