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생쥐의 骨髓細胞에 대한 Aminopterin 과 Amethopterin의 細胞遺傳學的 影響
양재섭,강봉석,최명철,김길웅 대구대학교 기초과학연구소 1987 基礎科學硏究 Vol.4 No.-
Aminopterin and amethopterin(Methotrexate; MTX) are antifolic drugs and anti-tumour agents, which inhibit DNA biosynthesis, were previously known to have mutagenic effects on various mammalian cells. Mutagenecity and toxicity of these agents have been determined by various methods. In the present study, effects of two anti-tumour agents on cultured mouse bone marrow cells were analyzed by observing chromosome aberrations and mitotic index. The results of the present investigation were as follows; 1. The chromosome aberrations analyzed were mainly chromosome gaps and breaks, chromatid gaps and breaks. In the Group-1 (treated for 8 hours), chromosome aberrations were not shown and it seems that mutagenic actions of anti-tumour agents were not aroused due to the short period of culture and the other culture conditions. However, in the Group-2 (treated for 24 hours), as the concentrations of antifolic compounds were increased, chromosome aberrations were increased. 2. In mitotic index, the Group-I treated with aminopterin and amethopterin showed marked inhibition in the mitotic index(O. 6% in aminopterin, 0.9% in amethopterin) at the high concentrations, compared with that of control (5. 2%). Both aminopterin and amethopterin showed a dose-dependent reduction of mitotic index. In the Group-2, each group treated with two antifolic compounds showed no marked differences in the mitotic index. compared with that of control. 3. Aminopterin was a more potent agent for induction of chromosome aberration than amethopterin.