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Mycobacterium fortuitum-chelonei complex의 간이 생물학적 분류동정
양석원,최철순,정상인,양용태 중앙대학교 의과대학 의과학연구소 1983 中央醫大誌 Vol.8 No.4
Recent outbreaks of non-tuberculous mycobacterial infections caused by M. fortuitum-chelonei complex (referred to MFCC) suggested that species and subspecies differentiation of the organisms is epidemiologically important. In this study, a simple dichotomous key for presumptive identification of the species of the MFCC by determining reactions to four biological tests, arylsulfatase test, iron-uptake test, 5% NaCl tolerance test and crocin reaction, was described. Arylsulfatase and iron-uptake positive test provides a rapid distinction of M. fortuitum from M. chelonei. M. fortuitum was further divided into biotype 1(bioval fortuitum) and biotype 2(biovar peregrinum) by crocin reaction. M. chelonei was further divided into biotype 1(subsp. abscessus) and biotype 2(subsp. chelonei) either by 5% NaCl tolerance or crocin reaction. These results indicated that four biological tests, i.e., arylsulfatase test, iron-uptake test, 5% NaCl tolerance test and crocin reaction could provide reliable means of presumptive differentiating the MFCC.