생쥐 정소 발달과정에 대한 미세구조적 연구

안영모,오승한,이정환,김완종 순천향대학교 기초과학연구소 2001 순천향자연과학연구 논문집 Vol.7 No.2

Differentiation and development of the mouse testis were studied by light and electron microscopes from the newborn up to the adult stage. The purpose of the present study was to investigate the morphological changes of developing germ cells in seminiferous tubule and Leydig cells in interstitial tissue during the developmental process of mouse testis. The results were as follows: the seminiferous tubule diameter began to increase from 1 day and was fully developed at the 30th day after birth, particularly, the seminiferous tubule diameter was rapidly increased between 15 days and 30 days of age. Germ cells of various stages were embedded in cytoplasm of the Sertoli cell. Leydig cell contained numerous mitochondria with tubular cristae and well developed smooth endoplasmic reticulum. Spermatogonia were located close to the basal layer. Spermatocytes were connected to each other by the intercellular bridge until the late spermatid stage. An acrosome located to the head apex and the microtubule arrangement of flagellum shown the typical "9+2" pattern. At the newborn stage, the Sertoli cell and germ cells started to be already differentiated. Spermatids and immature spermatozoa were appeared at 30 days of age, and thereafter, spermatogenesis occurred actively. In conclusion, the development of mouse testis was completed after 30 days of age at which the secondary sexual characteristics and the spermatogenesis began to occur. These results suggest that ultrastructural features of developing germ cells and Leydig cell were thought to have close interrelationship with their functional accomplishment.

간동맥 결찰이 간 철대사에 미치는 효과

안영모 부산의대 1970 부산의대잡지 - 부산대학교 의과대학 Vol.10 No.2

國家技術資格制度 - EDPS 分野 資格中心으로

안영모 한국정보과학회 1975 한국정보과학회 학술발표논문집 Vol.2 No.2

과학기술계인력의 개발정책 -제4차 5개년 계획을 중심으로-

안영모 한국발명진흥회 1976 特協 Vol.1 No.7

재생중인 흰쥐 간의 형태형성과 메탈로사이오닌 분포

안영모,김완종 순천향대학교 기초과학연구소 2004 순천향자연과학연구 논문집 Vol.10 No.2

Metallothionein(MT) is a low molecular weight (approximately 6,000~7,000Da), cysteine-rich protein (approximately 22~23%), metal-binding protein. MT is known to be induced by surgical operation and chemical agent, as carbon tetrachloride(CCI_(4)) and cadmium(Cd). The goal of the present study was to determine the distribution of the protein on regenerating rat liver after partial hepatectomy(PH). The results were as follow: Size of the remained liver increased at 1day after PH. Morphological changes were observed such as destroyed hepatocyte plate around central vein, enlarged nuclear cytoplasmic ratio and increased the number of hepatocytes with two nuclei. Expression of MT mRNA started to increase at 30 minutes, reached to maximum at 1 hour and maintained at 8 hours after PH. The level of MT mRNA showed similar to that of control group at 12 hours. On immunohistochemistry, in all groups treated with primary antibody, immunoreactivity indicating the presence of MT appeared moderately throughout both the cytoplasm and nucleus of rat hepatocyte. On the immunohistochemical analysis, immunoreactivities both in the cytoplasm and nucleus appeared after 4 hours, reached to maximum at 12 hours, and then it showed similar to those of normal control. These results suggested that the remained liver immediately entered cell proliferation and this MT protein during the process may be associated with transfer of factors needed to cell reproduction from the cytoplasm to the nucleus for regeneration of the liver after PH.

해운항만물류인력 양성 구조 분석에 관한 연구 : 부산지역을 중심으로

안영모,남기찬,곽규석 한국항해항만학회 2011 한국항해항만학회 학술대회논문집 Vol.2011 No.추계

일반적으로 국내 기업의 매출액 대비 물류비 비율은 미국, 일본 등 선진국에 비해 높기 때문에 여전히 물류관리 수준 향상을 위한 많은 노력이 필요한 상황이다. 그러나 우리나라는 물류관리 수준 향상을 위해서 가장 중요한 요소인 해운항만물류인력이 부족한 상황이며 수급현황을 살펴볼 때 더욱 부족해질 전망이다. 그래서 정부는 2005년부터 '해운항만물류전문인력 양성사업'을 시행하고 있는 상황이며, 인력양성의 주축인 대학 역시 관련 학과를 개설하며 해운항만물류인력의 부족 현상은 점차 완화될 것으로 기대된다. 이러한 상황에서 해운항만물류인력 양성의 구조적 측면을 살펴봄으로써 지금까지의 양성 현황에 대해 살펴 보고 앞으로의 양성 방향에 관해 연구해 볼 필요성에 따라 본 연구를 진행하였다.

햄스터 정자형성과정에 관한 미세구조적 연구

안영모,김완종,신길상 순천향대학교 기초과학연구소 2001 순천향자연과학연구 논문집 Vol.7 No.1

Ultrastructural changes during spermatogenesis in the hamster testis was studied by electron microscope. The purpose of the present study is to ascertain the ultrastructural changes of various germ cells, Sertoli and Leydig cells. The results were as follows: various staged germ cells were embedded in cytoplasm of Sertoli cell. Leydig cell contained numerous mitochondria with tubular cristae and well developed smooth endoplasmic reticulum. Spermatogonia were located close to the basal layer. They were spherical to somewhat oval cells with large nucleus. Spermatocytes were connected to each other until the late spermatid stage by intercellular bridge. An acrosome was located apical head and microtubule arrangement of flagellum was shown typical "9+2" characteristics. In conclusion, spermatogenesis involves the development, separation and maturation of germ cells, and Sertoli and Leydig cells show similar ultrastructures compared with ones of other mammalian species.

CNG 엔진 배출가스 저감용 삼원촉매 개발

안영모,박대성,주대환,이의성 대한환경공학회 2017 대한환경공학회 학술발표논문집 Vol.2017 No.11

Immunogold Labellings and Expression of Metallothionein in Regenerating Rat Liver

안영모,오승한,김호진,이미영,이종화,신길상,김완종,Ahn, Young-Mo,Oh, Seung-Han,Kim, Ho-Jin,Lee, Mi-Young,Lee, Jong-Hwa,Shin, Kil-Sang,Kim, Wan-Jong Korean Society of Electron Microscopy 2005 Applied microscopy Vol.35 No.1

진핵세포 내에 존재하는 metallothionein (MT)은 시스테인 함량이 높은 저분자량의 단백질로서 2가 금속이온들과의 친화력이 높아 중금속에 대한 해독작용, 금속이온의 대사 및 세포분열 등과 관련되어 있다. 본 연구자들은 간 재생능력이 우수한 흰쥐를 실험모델로 하여, 간의 70% 정도를 실험적으로 제거한 후, 간 재생을 유도하는 과정에서 시간 경과에 따라 간세포의 미세구조 변화와 더불어 MT 유전자 발현 및 이 단백질의 세포내 분포를 알아보고자 하였다. 부분 간 절제 후 간 조직이 증식되고 재구성되어 간이 원래의 크기로 재생되는 시간은 1주일 정도가 소요되었다. 재생중인 간세포는 핵대 세포질의 비가 크고, 핵내에 인이 크게 발달하고 크기가 작은 미토콘드리아가 다수 나타났으며, 조면소포체가 잘 발달하고 있었다. MT mRNA는 간 절제 직후부터 증가하기 시작하여 1시간 경과 후에 최대치에 이르렀고, 12시간 이후부터 감소하기 시작하였다. 재생중인 간세포에서 MT에 대한 면역반응은 간 절제 후 12시간이 경과한 군에서 가장 높게 나타났고, 이후 점차 감소하여 8일이 경과한 실험군에서는 정상 대조군과 유사한 정도로 감소하는 것으로 관찰되었다. 또한, 간 재생 초기에는 항-MT 금 입자들이 주로 세포질쪽에 분포하다가 점차 핵내에 존재하는 양이 증가하는 것으로 나타났다. 이러한 결과들은 간 절제 후 보상작용으로 일어나는 세포분열 단계에서 MT가 이 과정에 필요한 요소를 제공하는 역할을 수행하기 때문인 것으로 사료된다. Metallothionein (MT) is a low molecular weight, cysteine-rich, metal-binding, and non-enzymatic protein. The present study was carried out to investigate the expression of MT gene as well as the localization of MT in regenerating rat liver. In partial hepatectomized rats, MT mRNA was detected as early as 1 hr and reached a maximal level by 8 hr after the operation. Thereafter, this level decreased gradually until 24 hr, and it became similar to that of sham control. Meanwhile, time course of MT immunoreactivity using immunogold-labelling revealed that the number of gold particles in hepatocytes increased significantly by 12 hr, but decreased at 48 hr after partial hepatectomy. Ultrastructurally, immunogold particles indicating the presence of MT were distributed in both the cytoplasm and the nucleus of the rat hepatocytes, particularly the particles were distributed at rough endoplasmic reticulum and nucleolus and did not seem to adhere to mitochondria or lysosomes in proliferating hepatocytes. Briefly, high level of MT mRNA expression and the intense immunoreactivity and/or the specific localization of MT was observed during liver regeneration. These results suggest that MT possibly involves hepatocyte proliferation via the storage or the supply of various metal ions in the regenerating rat liver.

[특집논문] 외국언론의 선거보도(미국편)

안영모 언론중재위원회 1987 言論仲裁 Vol.24 No.-