원저 : Laser 침의 주파수 및 조사시간이 흰쥐의 염증성 부종에 미치는 영향 ( Original Article : Study on the Effects of Laser Acupuncture by the Changes of Frequencies and Radiant time Upon the paw Edema and Inflammation in Rats )

안병철(Byoung Choul Ahn),최문규(Moon Kyu Choi),박동석(Dong Suk Park),김재규(Jae Kyu Kim) 경희대학교 경희의료원 1986 慶熙醫學 Vol.2 No.3

유루증(流淚症)의 원인(原因).증상(症狀).치법(治法).침치료(鍼治療)에 관(關)한 문헌적(文獻的) 고찰(考察)

오기남,남상수,이재동,최도영,안병철,박동석,이윤호,최용태,O Gi-Nam,Nam Sang-Soo,Lee Jae-Dong,Choi Do-Young,Ahn Byoung-Choul,Park Dong-Seok,Lee Yun-Ho,Choi Yong-Tae 대한침구의학회 1998 대한침구의학회지 Vol.15 No.2

Purpose ; This Investigation was aimed to find out the Causes, Symptoms, Treatment, and Acupuncture of Epiphora(Watery Eye) in Oriental Medicine Methods ; 49 books were surveyed from <Neiching> to recent published books those referred to Epiphora. Results ; In Oriental Medicine, Epiphora is divided into two types; Cool Watery eye and Hot Watery eye. Both cool and hot type can be redivided into the mild and serious cases. The mild Watery eye means tears on the cheek with the wind; The serious Watery eye means tears on the cheek unrelated the wind from time to time. Conclusions ; The cause of Epiphora can be concluded with the shortage of functional division of Liver and Kidney(肝腎虛), and the chaos and fever of Liver meridian(肝經風熱). In Acupuncture agenst Epiphora, the acupuncture point at Bladder, Gallbladder and Liver meridian occupied the high level of the most often-used acupuncture point list; The acupuncture point at the portion of head and neck, upper extremities, lower extremities, and back, especially around orbit, were mainly used against Epiphora. Recently, beyond conventional acupuncture, Auricular- Acupuncture(耳鍼療法), Herb- Acupuncture(藥鍼療法), Bleeding-Acupuncture(潟血療法), Manipulation(手技療法) used against Epiphora.

부비동염(副鼻洞炎)의 근래(近來) 다양한 침구요법(鍼灸療法)에 대한 문헌적(文獻的) 고찰(考察)

김명희,남상수,이재동,최도영,안병철,박동석,이윤호,최용태,Kim Myung-Hee,Nam Sang-Soo,Lee Jae-Dong,Choi Do-Young,Ahn Byoung-Choul,Park Dong-Seok,Lee Yun-Ho,Choi Yong-Tae 대한침구의학회 1998 대한침구의학회지 Vol.15 No.2

Purpose: This study was carried out to investigate the research of acupuncture and moxibustion treatment for sinusitis. Methods: I mainly investigated 14 different kinds of recent edition of Chinese books and journals as references. Results: In the view of oriental medicine, sinusitis is regarded as Piyon(鼻淵). The loci used in the otopuncture therapies were Naepi(內鼻), Pye(肺), Aeck(額), Shinsangsun(腎上腺), Naebunpi(內分泌), and Weipi(外鼻) according to their frequencies. The drugs used in the drug-injection therapies were Corticoids, Lidocaine, Vitamin B, and herbal drugs. In addition, there were couple therapies mentioned in the references such as the electro-acupuncture therapies, the point penetration therapies, the point through-sinus acupuncture therapies, the point application therapies, the moxibustion therapies, the three-edged needle therapies, the ultra-sono acupuncture therapies, the cupping therapies, and the laser acupuncture therapies. Conclusions: There were various newly developed acupuncture and moxibustion treatment for sinusitis. Among them, the otopuncture therapies, the drug-injection therapies and the electro-acupuncture therapies were more frequently used than others.

고빈도전침자극(高頻度電鍼刺戟)의 자극시간(刺戟時間)에 따른 중추신경계(中樞神經系) 신경세포(神經細胞)의 활성변화(活性變化)에 미치는 영향(影響)

손성세,남상수,이재동,최도영,안병철,박동석,이윤호,최용태,Sohn Sung-Sae,Nam Sang-Soo,Lee Jae-Dong,Choi Do-Young,Ahn Byoung-Choul,Park Dong-Seok,Lee Yun-Ho,Choi Yong-Tae 대한침구의학회 1998 대한침구의학회지 Vol.15 No.2

The present study was designed to investigate the effect of different stimulation-duration of high frequency electroacupuncturet(EA) treatment on the neuronal activities in the spinal cord and brainstem using Fos immunohistochemical technique. Three different stimulus-duration was used in this experiment : 30minutes, 1 hour and 2 hours. The summerized results were summerized as follow : 1. The number of Fos expression was significantly increased in the spinal cord dorsal horn depending upon the increase of stimulus-duration (P<0.05). Otherwise, there was no significant difference between 30 minutes EA treated group and anesthetic control. 2. High frequency EA biphasic stimulation significantly enhanced the Fos expression in the DR, middle and rostral portion of PAG LD, and caudal PAG LV after 1 hour and 2 hours treatment. The number of Fos immunoreactive neuron in the brainstem was increased accorcting to the length of stimulus-duration. Those results indicate that at least 1 hour EA treatment was necessary to increase the neuronal activities in the spinal cord and brainstem. Those basic data from this study can be applied to establish the effective treatment of EA for pain control in the clinical field.

봉독약침(蜂毒藥鍼)이 척수내(脊髓內) 통증관련(痛症關聯) 신경세포(神經細胞)의 활성(活性)에 미치는 영향(影響)

정선희,이재동,고형균,안병철,최도영,박동석,Jeong, Sun-Hee,Lee, Jae-Dong,Koh, Hyung-Kyun,Ahn, Byoung-Choul,Choi, Do-Young,Park, Dong-Suk 대한침구의학회 2000 대한침구의학회지 Vol.17 No.2

Introduction : In spite of the use of Bee Venom aqua-acupuncture in the clinics, the scientific evaluation on effects is not enough. Bee Venom aqua-acupuncture is used according to the stimulation of acupuncture point and the chemical effects of Bee Venom. The aims of this study is to investigate the analgegic effects of the Bee Venom aqua-acupuncture, through the change of writhing reflex and the change of c-fos in secondary neurons in the spinal cord. Materials and Methods : Pain animal model was used acetic acid method. The changes of writhing reflex of the mice which were derived pain by injecting acetic acid into the abdomen, after stimulating Bee Venom aqua-acupuncture on Chungwan(CV12) were measured. We used Fos immunohistochemical technique to study the neuronal activity in the spinal cord. Results : 1. Expression of c-fos in superficial dorsal horn(SDH), nucleus proprius(NP) and neck of dorsal hom(N) on 6~9th thoracic spine decreased significantly at $2.5{\times}10-4$g/kg Bee Venom aqua-acupuncture, compared with saline-acetic acid group. 2. The numeral change of Fos-LI neurons on the NP, N, and ventral gray(V) on 6-9th thoracic spine, SDH on 9-11th thoracic spine, and SDH and V on 11~13th thoracic spine decreased significantly at Chungwan(CV12) Bee Venom aqua-acupuncture, compared with saline-acetic acid group. 3. The correlation between the numbers of writhing refleax and Fos-LI neurons in T6-13 segment was statistically statistically significant at Chungwan(CV12) Bee Venom aqua-acupuncture. Conclusion : This study shows that the Bee Venom aqua-acupuncture on Chungwan(CV12) decreases the numbers of Fos-LI neurons. As the analgegic effects of Bee Venom aqua-acupuncture is recognized. Bee Venom aqua-acupuncture treatment is expected for pain modulation. In order to use it in many ways, more researches are needed for the dose and stability of Bee Venom aqua-acupuncture.

애구(艾灸)의 연소(燃燒) 구간별(區間別) 열량비(熱量比)에 대(對)한 연구(硏究)

강기원,남상수,이재동,최도영,안병철,박동석,이윤호,최용태,Kang Ki-Weon,Nam Sang-Soo,Lee Jae-Dong,Choi Do-Young,Ahn Byoung-Choul,Park Dong-Seok,Lee Yun-Ho,Choi Yong-Tae 대한침구의학회 1998 대한침구의학회지 Vol.15 No.2

In order to evaluate calorie rate of moxa-combustion(direct moxibustion=DM, indirect moxibustion with stainless steel tube=IMS, indirect moxibustion with ginger slice=IMG), calorie rate(%) in the preheating period, heating period, retaining period, and cooling period was calculated respectively to the total combustion calories in all the periods. The result are as follow: 1. Indirect moxibustion with stainless steel tube had the highest rate with the statistical significance than other groups during the preheating period, represented statistical differences between IMS group and IMG group, and also between DM group and IMS group. 2. Indirect moxibustion with ginger slice group showed the highest rate with the statistical significance and followed by DM group and IMG group during the heating period, represented statistical difference among DM, IMS, and IMG group. 3. Direct moxibustion group had the highest rate with the statistical significance than other group during the retaining period, represented statistical difference between DM group and IMG group, and also between DM group and IMS group. 4. Indirect moxibustion with stainless steel tube had the highest rate with the statistical significance than other groups during the cooling period, represented statistical differences between IMS group and IMG group, and also between IMS group and DM group.

Jaw Opening Reflex 및 RT-PCR을 이용한 봉독의 진통효과

조광호,이재동,박동석,안병철,Cho, Kwang-Ho,Lee, Jae-Dong,Park, Dong-Suk,Ahn, Byoung-Choul 대한약침학회 2000 Journal of pharmacopuncture Vol.3 No.1

The purpose of this study is to prove the analgesic effects of apitoxin and its mechanism via jaw-opening reflex(JOR) and measuring expression of mRNA in Phospholipase and Tryptophan hydroxylase(TPH) using RT-PCR. The experiments were carried out on Sprague-Dawley rats(300-400g) and mastocytoma(P-185 HTR) for JOR and RT-PCR, respectively. Rats anesthetized with thiopental sodium (80mg/kg) were used in the Tooth Pulp stimulation induced JOR. The amplitude of a digastric electromyogram (dEMG) was recorded during the stimulation at an intensity of 1.5 times the threshold for JOR. Apitoxin used in this experiment was diluted with normal saline by 1:1000. Apitoxin was injected intravenously into the test group while normal saline to the control group. However, it was injected directly into the cell of mastocytoma. We referred to base sequence registered in Genbank in designing primers for RT-PCR. The results were as follows; (1)Compared with control group, analgesic effect started to show right after Sprague-Dawely rats were treated with apitoxin($71.50{\pm}8.08$) and lasted for 50 minutes. (2)As a result of the experiment of RT-PCR, we witnessed significant changes in the degree of expression of phospholipase or rate-limiting enzyme of biosynthesis of prostaglandins with $10{\mu}g/ml$ apitoxin.($31.74{\pm}18.98%$, P<0.05) (3)As a result of the experiment of RT-PCR, we witnessed significant changes in the degree of expression of TPH or rate-limiting enzyme in biosynthesis of serotonin with $10{\mu}g/ml$ apitoxin.($131.37{\pm}16.87%$, P<0.05). These results suggest that $10{\mu}g/ml$ apitoxin have the most analgesic effects. This study showed that apitoxin has analgesic effects and held good for 50 minutes. The injection of apitoxin has brought out changes in the degree of expression of phospholipase and TPH. These results strongly suggest that analgesic mechanism by apitoxin is closely related to prostaglandins and serotonin.

원저 : 대추혈의 침구 및 Laser 침자극이 발열가토에 미치는 영향 ( Original Article : A Study on the Antipyretic Effect of Acupuncture , Moxibustion and Laser Acupuncture at Dae - Toe ( GV 14 ) in Rabbit )

남윤석(Yoon Seok Nam),안병철(Byoung Choul Ahn),최용태(Yong Tae Choe),이윤호(Yun Ho Lee) 경희대학교 경희의료원 1986 慶熙醫學 Vol.2 No.2

四君子湯 및 四物湯 藥鍼이 免疫反應에 미치는 影響

李秉烈(Byung Ruyl Lee),安秉哲(Byoung Choul Ahn) 대한한의학회 1997 대한한의학회지 Vol.18 No.1

봉독약침(蜂毒藥鍼)의 항암효과(抗癌效果)에 대한 분자생물학적(分子生物學的) 연구(硏究)

박찬열,서정철,최도영,안병철,Park, Chan-Yol,Seo, Jung-Chul,Choi, Do-Young,Ahn, Byoung-Choul 대한약침학회 2000 Journal of pharmacopuncture Vol.3 No.1

To study anti-cancer effect and molecular biological mechanism of bee venom for aqua-acupuncture, the effects of bee venom on cell viability and apoptosis were analyzed using MTT assay, tryphan blue assay, $[^3H]$thymidine release assay, flow cytometric analysis, and activity of caspase-3 protease activity assay. To explore whether anti-cancer effects of bee venom are associated with the transcriptional control of gene expression, quantitative RT-PCR analysis of apoptosis-related genes was performed. The obtained results are summarized as follows: 1. The MTT assay demonstrated that cell viability was decreased by bee venom in a dose-dependant manner. 2. Significant induction of apoptosis was identified using tryphan blue assay, $[^3H]$thymidine release assay, and flow cytomet1 ric analysis of sub $G_1$ fraction. 3. In analysis of caspase-3 protease activity, the activity had increased significantly, in a dose-dependant manner. 4. Quantitative RT-PCR analysis of the apoptosis-related genes showed that Bcl-2 and Bcl-$X_L$ were down-regulated whereas Bax was up-regulated by bee venom treatment.