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Microwave Plasma Sterilization System을 이용한 배추 절임수의 미생물 저감화
유동진(Dong Jin Yu),신윤지(Yoon Ji Shin),김현진(Hyun Jin Kim),송현정(Hyeon Jeong Song),이지혜(Ji Hye Lee),장성애(Sung Ae Jang),전소정(So Jung Jeon),홍순택(Soon Taek Hong),김성재(Sung Jae Kim),송경빈(Kyung Bin Song) 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.1
김치 제조 공정에서 사용되는 김치 절임수의 미생물학적 안전성 확보와 재활용을 위한 연구로써, 본 연구에서는 microwave plasma sterilization system을 이용한 김치 절임수의 미생물 수 저감화를 위하여, E. coli O157:H7, L. monocytogenes, S. Typhimurium에 대한 살균 효과를 측정하고, 또한 사용한 김치 절임수에 본 장치를 적용하였다. 김치 공장에서 반복 사용한 절임수에 있는 coliform, E. coli, Salmonella spp., total aerobic bacteria, yeasts and molds가 사용횟수가 늘어남에 따라 미생물 수가 증가하였다. Microwave plasma를 이용한 살균처리에서는 E. coli O157:H7, L. monocytogenes, S. Typhimurium의 D₁?-value가 0.48, 0.52, 0.45 cycle로 각각 측정되었고, 또한 1회 사용한 절임수에 microwave plasma sterilization system 적용 시, coliform, E. coli, Salmonella spp., total aerobic bacteria, yeasts and molds 숫자가 유의적으로 감소하였다. 따라서 본 연구 결과, 김치공장의 김치 절임수를 재사용하기 위해서 microwave plasma sterilization system을 이용한 살균방법이 적합하다고 판단된다. This study was conducted to decrease the microbial hazard in kimchi saline water with microwave plasma sterilization system and to evaluate the inactivation of foodborne pathogens by the microwave plasma sterilization system as a non-thermal treatment. Contamination of coliform, Escherichia coli, and yeasts and molds were detected in the used saline water, and the microbial populations increased as the saline water was reused repeatedly. The D₁?-values of E. coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes by the microwave plasma sterilization system were 0.48, 0.52, and 0.45 cycle, respectively. In addition, the microbial populations of coliform, E. coli, Salmonella spp., total aerobic bacteria, and yeasts and molds in the used kimchi saline water were significantly decreased by treating the saline water using the microwave plasma sterilization system. Therefore, these results suggest that microwave plasma sterilization system can be useful in improving the microbial safety of the used saline water.
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유동진(Dong Jin Yu),최동원(Dong Won Choi),신윤지(Yoon Ji Shin),송혜연(Hye Yean Song),송경빈(Kyung Bin Song) 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.8
옥수수수염 차의 MPSS 처리에 의한 위해미생물의 살균 효과에 대하여 연구하였다. 옥수수수염 차에 E. coli와 L. monocytogenes를 5~6 log CFU/㎖ 수준으로 접종하고, MPSS 처리한 후 25℃에서 12일 동안 저장하면서 실험하였다. E. coli는 1, 2, 3 회 MPSS 처리 후 각각 1.14, 2.49, 5.72 log CFU/㎖의 감균 효과를 보였고, L. monocytogenes는 MPSS 1, 2, 3회 처리 후 각각 1.93, 4.49, 6.62 log CFU/㎖의 감균 효과를 보였다. 또한, E. coli와 L. monocytogenes 모두 MPSS 4회 처리 후에는 모두 사멸되었고, 저장 12일까지 검출되지 않았다. 그리고 MPSS 처리는 옥수수수염 차의 TPC 에 부정적인 영향을 끼치지 않았고, 탁도는 4회 이상 MPSS 처리에 의해 향상되었다. 따라서 본 연구 결과, MPSS 처리는 옥수수수염 차의 품질 유지와 더불어 미생물 생육 억제가 가능하기에 옥수수수염 차의 살균에 MPSS를 적용할 수 있다고 판단된다. Inactivation of foodborne pathogenic bacteria in corn silk tea was evaluated using a microwave plasma sterilization system (MPSS). Corn silk tea was inoculated with Escherichia coli and Listeria monocytogenes, treated with an MPSS treatment, and stored at 25℃ for 12 days. The one, two, and three cycles of treatment with MPSS reduced the population of E. coli by 1.14, 2.49, and 5.72 log CFU/㎖, respectively, compared to that of the control. In the case of L. monocytogenes, one, two, and three cycles of MPSS treatment reduced the population by 1.93, 4.49, and 6.62 log CFU/㎖, respectively. Both E. coli and L. monocytogenes were eliminated within four cycles of treatment with MPSS, and even after 12 days of storage, the bacteria were not detected. Total polyphenol content in the corn silk tea did not change much among treatments, and turbidity of the corn silk tea improved following four cycles of MPSS treatment. These results suggest that MPSS treatment can be useful for improving the microbial safety and quality of corn silk tea during storage.
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이산화염소수를 이용한 계사 내 깔짚의 미생물 수 저감화
유동진 ( Dong Jin Yu ),김현진 ( Hyun Jin Kim ),송현정 ( Hyeon Jeon Song ),신윤지 ( Yoon Ji Shin ),채현석 ( Hyun Seok Chae ),송경빈 ( Kyung Bin Song ) 한국식품저장유통학회(구 한국농산물저장유통학회) 2011 한국식품저장유통학회지 Vol.18 No.1
We evaluated microbial inactivation in chicken cage litter, to ensure microbial safety, using aqueous chloride dioxide. Contamination by coliforms, Escherichia coli, Listeria spp., yeasts and molds, total aerobic bacteria, and Salmonella spp. was detected in fresh cage litter, and microbial populations increased if litters were repeatedly used. Aqueous ClO2 treatment (500 ppm) significantly decreased the populations of coliforms, E. coli, Listeria spp., yeasts and molds, total aerobic bacteria, and Salmonella spp. in all litter samples tested. In particular, aqueous ClO2 treatment on fresh litter reduced the initial populations of coliform, E. coli, Listeria spp., yeasts and molds, and total aerobic bacteria by 4.47, 1.29, 1.23, 3.24, and 5.2 log CFU/g, respectively. In addition, when litters used for 1 and 5 weeks were tested, treatment significantly reduced microbial populations. The results suggest that aqueous ClO2 treatment is useful to reduce microbial hazards in chicken cage litter and to improve the microbial safety of slaughtered chickens.
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Hollow-Fiber LPME를 이용한 잔류농약 검출
조현우,이상섭,신윤지,장미애 경기대학교 기초과학연구소 2008 기초과학논문집 Vol.21 No.-
A method for the determination of trace amount of pesticides in aqueous samples using hollow fiber-liquid phase microextraction(HF-LPME) and capillary gas chromatograph/micro electron capture detector(GC/μ ECD) has been established. HF-LPME method has been optimized with respect to several experimental parameters including the effects of extraction solvent, extraction time and extraction temperature for the determination of pesticides from aqueous samples. The calibration curves for pesticides showed good linearity (above r²=0.98). The established method is feasible for the determination of trace amounts of pesticides in several aqueous sample. The limit of detection(LOD) and the limit of quantitation(LOQ) have been found to be 0.05~1.0 and 0.5~2.5 ng/mL, respectively. The established HF-LPME method for the analysis of pesticides from aqueous sample can be used for the determination of biological, food and environmental samples.
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