인간 대장암 세포주에서 sulindac sulfide 처리에 의해 차별적으로 발현되는 유전자 군의 분석

신승화,김종식,Shin, Seung-Hwa,Kim, Jong-Sik 한국생명과학회 2007 생명과학회지 Vol.17 No.7

본 연구에서는 NSAID계 약물인 sulindac, sulindac sulfone, 그리고 sulindac sulfide 처리에 의한 암세포 생존율에 미치는 영향을 확인하기 위하여, 인간 대장암 세포주인 HCTl16에 각각 10 ${\mu}M$의 NSAID들을 처리하였다. 처리한약물 중 sulindac sulfide에 의한 암세포 생존율이 가장 높게 감소하는 것으로 MTS assay 결과 확인되었다. 또한 sulindac sulfide의 처리 농도가 증가됨에 따라 세포 생존율이 감소하는 것으로 확인되었다. Sulindac sulfide의 처리에 따른 이러한 암 세포 사멸의 분자생물학적 기전을 이해하기 위하여, oligo DNA microarray 실험을 수행하였다. 그 결과, 10 ${\mu}M$의 sulindac sulfide의 처리에 의해 2배 이상 발현이 증가되는 유전자가 23개 확인되었고, 반대로 2배 이상 발현이 감소되는 유전자가 33개 확인되었다. 증가되는 유전자중 3개(NAG-1, DDIT3, PCK2)를 선택하여, RT-PCR과 real-time PCR을 수행하였다. 그 결과 두 실험 모두 DNA microarray 실험결과와 동일하게 발현이 증가되었다. 이 중 sulindac, sulindac sulfone, sulindac sulfide에 의 한 NAG-1 유전자의 발현변화를 RT-PCR과 real-time PCR 방법으로 확인한 결과, sulindac sulfide에 의한 암 억제유전자인 NAG-1의 발현이 가장 많이 발현되었다. 이러한 연구결과는 세포생존율 결과와 비교하였을 때, NAG-1의 높은 발현과 암 세포 생존율의 감소가 관련이 있음을 간접적으로 시사한다. 따라서 이들 연구결과는 sulindac sulfide에 의한 화학적 암 예방법의 분자생물학적 기전을 이해하는데 도움을 줄 것으로 생각한다. To investigate whether sulindac, sulindac sulfone, and sulindac sulfide could affect cancer cell viabilities, human colorectal HCTl16 cells were treated with 10 ${\mu}M$ of each NSAID. Among treated NSAms, sulindac sulfide dramatically decreased the cell viabilities detected by MTS and the cytotoxic effect showed dose-dependent manner. To understand the molecular mechanism of cell death in response to sulindac sulfide treatment, we performed oligo DNA microarray analysis. We found that 23 genes were up-regulated more than 2 folds, whereas 33 genes were down-regulated more than 2 folds by treatment of 10 ${\mu}M$ sulindac sulfide. Among the up-regulated genes, we selected 3 genes (NAG-1, DDIT3, PCK2) and performed RT-PCR and quantitative real-time PCR to cofirm microarray data. The results of RT-PCR and real-time PCR were highly accorded with those of microarray experiment. As NAG-1 is well-known gene as tumor suppressor, we detected changes of NAG-1 expression by 10 ${\mu}M$ of sulindac, sulindac sulfone, and sulindac sulfide. The results of RT-PCR and quantitacve real-time PCR indicated that sulindac sulfide was the strongest inducer of NAG-1 among treated NSAIDS. This result implies that induction of NAG-1 by sulindac sulfide plays important role in cell death of colorectal cancer. Overall, we speculate that these results may be helpful in understanding the molecular mechanism of the cancer chemoprevention by sulindac sulfide in human colorectal cancer.

상피성 난소암에 있어서 증식세포핵 항원의 예후인자로서의 의의

신승화 ( Seung Hwa Shin ),안준모 ( Joon Mo Ahn ),김동휘 ( Dong Hwai Kim ),박은동 ( Un Dong Park ) 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.12

Proliferating cell nuclear antigen(PCNA) is a stable cell cycle related antigen. PCNA is considered to be a marker of cell proliferation. The aim of this study was to evaluate the possible correlation between the expression of PCNA and grade, stage and survival in epithelial ovarian cancers. The expression was investigated by immunohistochemical method(ABC) on 50 cases of epithelial ovarian cancer. The material consisted of serous cystadenocarcinoma 60%(30/50), mucinous cystadenocarcinoma 36%(18/50) and endometroid carcinoma 4%(2/50). The PCNA labelling index(LI) ranged from 7.5% to 92.5% with a median value of 44.9%. In the grade 1 tumor, PCNA LI was 37.5% and in the grade 2 & 3 tumors PCNA LI were 58.6% and 78%, retrospectively(p<0.05). Also, a positive correlation was found between PCNA LI and clinical stages(p<0.05). For patients with PCNA LI greater than the median(high proliferation group), the estimated 3-year survival rate was 44.4% ; for patients with PCNA LI below the median(low proliferative group), the estimated 3-year survival rate was 73.9%(p<0.05). In conclusion, PCNA LIis one of the valuable prognostic factors of epithelial ovarian cancer.

시뮬레이션기반 전문심장소생술교육이 간호학생의 지식, 수행능력에 미치는 효과

신승화(Shin, Seung-Hwa),권말숙(Kwon, Mal-Suk),권상민(Kwon, Sang-Min) 한국산학기술학회 2013 한국산학기술학회논문지 Vol.14 No.11

연구목적: 본 연구는 미국심장협회 ACLS provider course의 시뮬레이션기반 전문심장소생술 교육이 간호학생 의 지식 및 수행능력에 미치는 효과를 검증하기 위함이다. 연구방법: 연구대상은 D대학 졸업학년 간호학생 22명이다. 수집된 자료는 SPSS/WIN 18.0 program을 이용하여 분석하였다. 연구결과: 시뮬레이션기반 전문심장소생술 교육을 받은 후 교육 전보다 간호학생의 지식 및 수행능력 점수가 유의하게 높아졌다. 결론: 시뮬레이션기반 전문심장소생술 교육은 간호학생의 지식 및 수행능력을 향상시키는데 효과적임을 검증하였으므로 간호학생 대상의 표준화된 응급간 호 교육프로그램 개발에 기초자료를 제공할 수 있다고 본다. 또한 전문심장소생술 교육에 다양한 학습방법을 적용한 후 비교분석하는 후속연구와 지식 및 수행능력 지속성 연구와 같은 종적연구를 제언한다. Purpose: The purpose of this study was to examine the effects of the simulation-based training for advanced cardiovascular life support on the knowledge and competence of nursing students. Method: The subjects were 22 nursing students, who ungraduated D university. Data were analyzed with SPSS/Win 18.0 program. Results: There was a significant improvement in knowledge and competence after receiving than before training. Conclusion: Simulation-based training for advanced cardiovascular life support is effective strategy for increasing the knowledge and competence of nursing students and will can provide basic data in development of standardized emergency nursing education programs. Further research is needed in the comparative analysis about various learning of advanced cardiac life support education and a longitudinal study of maintenance the knowledge and competence.

풍자원 평가를 위한 건축물 주변의 유동특성

조강표,정승환,신승화,Cho, Kang-Pyo,Jeong, Seung-Hwan,Shin, Seung-Hwa 한국유체기계학회 2011 한국유체기계학회 논문집 Vol.19 No.3

To utilize wind resources effectively around buildings in urban area, the magnitudes of wind velocity and turbulence intensity are important, which means the need of the information about the relationship between the magnitude of wind velocity and that of fluctuating wind velocity. In the paper, wind-tunnel experiments were performed to provide the information about Characteristic of Wind flow around buildings with the spanwise distance and the side ratio of buildings as variables. For a single building with the side ratios of one and two, the average velocity ratio was 1.4 and the velocity standard deviation ratio ranged from 1.4 to 2.6 at the height of 0.02m at the corner of the windward side, in which flow separation occurred. For twin buildings with the side ratios of one and two, the velocity ratio ranged from 2 to 2.5 as the spanwise distance varied at the height of 0.02m, and the velocity standard deviation ratio varied near 1.25. For twin buildings with the side ratios of one and two, the maximum velocity ratio was 1.75 at the height of 0.6m, and the maximum velocity standard deviation ratio was 2.1. It was also found from the results of CFD analysis and wind-tunnel experiments that for twin buildings with the side ratios of one and two, the difference between the velocity ratio of CFD analysis and that of wind-tunnel experiments at streamwise distances was near 0.75.

교대근무 간호사의 건강증진행위가 피로와 우울에 미치는 영향

이은주(Lee, Eun-Joo),신승화(Shin, Seung-Hwa) 한국산학기술학회 2014 한국산학기술학회논문지 Vol.15 No.4

본 연구는 교대근무 간호사의 건강증진행위,피로 및 우울을 파악하고,건강증진행위가 피로와 우울에 미치는 영향을 밝히기 위한 서술적 연구이다.대상자는 경상북도 A시에 소재한 3개 종합병원에 근무하는 243명의 간호사로,자료수집기간 은 2012년 9월 19일부터 25일까지였다.자료의 분석은 SPSS 20.0을 사용하여 descriptivestatistics,t-test,Pearson’s correlationcoefficients,ANCOVA,및 Stepwisemultipleregression으로 분석되었다.그 결과 교대근무 간호사의 건강증진행 위 점수는 모든 항목에서 비교대근무 간호사 보다 낮았고 피로와 우울에서는 높게 나타났다.교대근무 간호사의 피로에 영향 을 미치는 건강증진행위로는 영적성장 및 스트레스 관리로 11%의 설명력을 보였고,우울에 영향을 미치는 건강증진행위로는 신체활동 및 대인관계로 5%의 설명력을 나타내었다.이상의 결과를 바탕으로 기관차원에서 교대근무 간호사의 근무 형태를 고려한 맞춤형 건강증진행위를 증진시킬 수 있는 방안 마련과,피로와 우울 중재를 통한 간호 업무성과 향상을 위한 영적성 장,대인관계,신체활동 및 스트레스관리 항목이 강화된 전문적인 건강관리 프로그램 개발을 제언하는 바이다. This study was a descriptive study to identify the effect of health promoting behavior on fatigue and depression and to investigate these levels among nurses. Data were collected from September 19th 2012 to September 25th 2012 by questionnaires from 243 hospital nurses of 3 hospitals in Kyungsang-Bukdo. The data was analyzed using descriptive statistics, t-test, Pearson’s correlation coefficients, ANCOVA, and Stepwise multiple regression. The result showed that in health promoting behavior, shift nurses had higher scores than it of non-shift nurses. The health promotion behavior predictors of fatigue were spiritual growth and stress management and the predictors of depression were physical activity and interpersonal relationship. This results suggest that development of professional health promoting program which is strengthened these parts of spiritual growth, stress management, physical activity and interpersonal relationship, is needed.

풍자원 평가를 위한 건축물 주변의 유동특성

조강표(Kangpyo Cho),정승환(Seung-Hwan Jeong),신승화(Seung-Hwa Shin) 한국유체기계학회 2011 한국유체기계학회 논문집 Vol.14 No.3

풍자원 평가를 위한 건축물 주변의 유동특성

조강표(Kangpyo Cho),정승환(Seung-Hwan Jeong),신승화(Seung-Hwa Shin) 한국유체기계학회 2010 유체기계 연구개발 발표회 논문집 Vol.2010 No.12

To utilize wind resources effectively around buildings in urban area, the magnitudes of wind velocity and turbulence intensity are important, which means the need of the information about the relationship between the magnitude of wind velocity and that of fluctuating wind velocity, In the paper, wind-tunnel experiments were performed to provide the information about Characteristic of Wind flow around buildings with the span wise distance and the side ratio of buildings as variables. For a single building with the side ratios of one and two, the average velocity ratio was 1.4 and the velocity standard deviation ratio ranged from 1.4 to 2.6 at the height of 0.02m at the corner of the windward side, in which flow separation occurred. For twin buildings with the side ratios of one and two, the velocity ratio ranged from 2 to 2.5 as the spanwise distance varied at the height of 0.02m, and the velocity standard deviation ratio varied near 1.25. For twin buildings with the side ratios of one and two, the maximum velocity ratio was 1.75 at the height of 0.6m, and the maximum velocity standard deviation ratio was 2.1. It was also found from the results of CFD analysis and wind-tunnel experiments that for twin buildings with the side ratios of one and two, the difference between the velocity ratio of CFD analysis and that of wind-tunnel experiments at streamwise distances was near 0.75.

도시내 건축물 주변의 풍자원 평가

조강표(Kang Pyo Cho),정승환(Seung Hwan Jeong),홍성일(Sung Il Hong),신승화(Seung Hwa Shin) 한국유체기계학회 2009 유체기계 연구개발 발표회 논문집 Vol.2009 No.-

Wind velocity magnitude around buildings can be used to assess pedestrians' comport and wind energy. This paper's main purpose is to give basic ideas that may be used for wind resource assessment around buildings. In this paper, side ratio and span wise distance were selected as variables which affect wind velocity variation around buildings. Then wind velocity increasement ratios at the heights of pedestrian, small wind turbine installed in a roadlamp and the roof level by CFD analysis. It was shown that maximum wind velocity increasement ratios were obtained at the height of pedestrian about 116% and 148% for the side ratios of 1 and 2. Respectively at the height of small wind turbine installed in a roadlamp, maximum wind velocity increasement ratio was 95.3% for the side ratio of 2. Wind Velocity increasement ratios at the roof level were about 19% to 21% for all the side ratios. Locations at which maxim velocity increasement ratios were obtained were not the same for each height.

루지의 형상변화가 공력성능에 미치는 영향

한보현(Bo-Hyun Han),김환(Hwan Kim),박종혁(Jong-Hyeok Park),차진혁(Jin-Hyeok Cha),김용환(Yong-Hwan Kim),신승화(Seung-Hwa Shin),정한솔(Han-Sol Jeong),김광용(Kwang-Yong Kim) 대한기계학회 2016 대한기계학회 춘추학술대회 Vol.2016 No.12

Numerical study on the aerodynamic performance of a luge has been performed for various shapes of luge body. To analyze the aerodynamic drag of the luge, three-dimensional Reynolds-Averaged Navier-Stoke equations were used with the SST model as a turbulence closure. Grid system was composed of unstructured tetrahedral meshes. The effects of the major axis length of the front bottom, the minor axis length of the cover between the legs, the minor axis length of curved surface of the shoulder bottom, and the radius of the shoulder cover on the drag coefficient, were evaluated. The results show that the aerodynamic performance was most affected by the radius of curvature on shoulder cover.