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Oligodeoxyribonucleotide의 화학적 합성
송병수,이상직,김종대,Byong-Soo Song,Lee Sang-Jik,Jong-Dae Kim 대한화학회 1986 대한화학회지 Vol.30 No.1
"Phosphotriester Method"에 따라 모형 oligodeoxyribonuclotide로서 EcoRI인식자리를 지닌 육량체 GpApApTpTpCp를 화학적으로 합성하면서, 이 과정에 포함되는 반응물의 보호, 축합 보호기의 제거, 생성물의 정제, 정량 및 확인작업의 전반에 걸쳐 기존 방법에 대한 평가와 개선을 수행하였다. 소량체합성의 기본 단위는 이량체로 함이 효유적이며, 착합단계의 수득율은 이량체>사량체>육량체의 순으로 감소함을 알았다. UV/IR분광법 및 hplc/tlc크로마토그래피법을 이용함으로써 미량의 시료를 사용하여 Oligonucleotide를 합성함에 수반되는 정량/확인의 난점을 해결하였다. 또한 5'-OH가 노출된 nucleotide 중 전자를 과량 사용하여 반응시키고 산처리하여 탄산수소나트륨 수용액으로 세척하는 것이 양호한 방법임을 알았다. 아울러 합성에 관련된 nucleotide와 그 유도체를 분광학적 크로마토 그래피자료를 수록하였다. Along with synthesizing a model oligodeoxyribonuclotide GpApApTpTpCp which had the EcoRI recognition site according to "Phosphotriester Method", reported methods for protecting functions, condensation, deprotection, purifying products, quantification and identification were evaluated and modified. It was realized that oligomers were efficiently synthesized by elongating by dimer unit per step of the condensation and the condensation yields were decreased with increased numbers of the residues. The problems of quantification/identification involved in the oligonucleotide synthesis using small amount of the reactants were solved by employing UV/IR spectrophotometry and hplc/tlc. It was also proved that a nucleotide having the exposed 5'-OH function was well synthesized by condensing a 5'-OH nucleotide and a phosphodiester nucleotide whose molar ratio was intendedly made to be 1 : 1.2 and then detritylating followed by washing with an aqueous solution of sodium bicarbonate. Spectrophotometric and chromatographic data of the nucleotides and their derivatives concerned in this synthetic work were prepared.
송병수,원치규 대한외상학회 1992 大韓外傷學會誌 Vol.5 No.1
This is a retrospective clinical study of 195 cases of abdoinal trauma treated at the Department of Surgery, Hanyang University Hospital during the period from july 1981 to june 1991. The results are as follow ; 1) The sex distribution was 141 males (72.3%) and 54 females (27.7%) with the ratio of 2.6 : 1, and by age, high incidence was seen in the 3rd and the 4th decade(54.4%). 2) The seasonal distribution was not characteristic, but the hourly distribution showed the high incidence from day to evening in blunt trauma, evening to night in penetrating trauma. 3) Among the 195 cases of trauma, blunt trauma was 128 cases (65.6%) and penetrating trauma 67 cases (34.4%), with the ratio of 1.9:1. Traffic accident was most common in the former, stab would in the later. 4) The time lapse between trauma and arrival at hospital was predominantly within 2 hours (40.5%). The operation was predominantly performed from 8 hours to 12 hours (34.7%) after trauma. 5) The clinical manifestations were consisted of abdominal pain (88.7%), tenderness (82.6%), rebound tenderness (50.3%), rigidity (42.6%), and decreased peristalsis (34.3%) in order of frequency. 6) The preoperative value of Hb and WBC were below 12gm/dl in 53.4%, and above 10.000/ml in 77.0% respectively. The result of paracentesis was positive in 79.2% (sensitivity ; 82.1%, specificity ; 41.7%). 7) Among the diagnostic measures used to determine the surgical indications, the clinical manifestations were most commonly used in 32.3% (22.6% in blunt trauma, 50.7% in penetrating trauma). CT in 25.1%, paracentesis in 24.1%, and ultrasonography in 18.5%. 8) The abdominal organs injured were spleen (22.7%), liver(16.7%), mesentery (13.1%), small bowel injury. Multiple organ injury was 48.6% in blunt trauma, 22.7% in penetrating trauma. 9) The associated extra-abdominal injuries were seen in 52.3% of cases suffered abdominal organ injury ; in 61.9% of blunt trauma, and in 29.5% of penetrating trauma. They were head injury (42.8%), chest injury (23.6%) low extremity fracture (10.9%), pelvic bone fracture (6.9%) in order of frequency. 10) Of the total 195 patients, operation was performed in 167 cases (85.6%), and 28 cases (14.4%) were treated conservatively. 11) Transfusion of blood was done in 144 cases (73.8%), and not required in 51 cases (26.2%) 12) Post-operative complications occurred in 54 cases (32.3%), and pulmonary complication was most common. 13) The mortality rate was 5.1% (10 cases), and the causes of death were hypovolemia, sepsis, and renal failure in order of frequency.