Insecticidal activities of Ginkgo biloba seed coat extracts against Spodoptera exigua (Lepidoptera: Noctuidae)

소상섭,황태환,Waseem AKRAM,최종경,이종진 한국곤충학회 2012 Entomological Research Vol.42 No.3

The present study relates to a methanol extract of the seed coat of Ginkgo biloba, and tested particularly on the third instar larvae of Spodoptera exigua. The extract was found to have an inhibitory effect on the growth of the larvae besides bringing a change in the nutrient reserves in the body of the insect. Topical application of five different doses of the methanol extract resulted in a mortal effect to third instar larvae of S. exigua that is very much dependent on the dose as well as duration of exposure. Lower doses revealed lower mortality after 24 h of application. At doses of 1.00, 2.00, 4.00, 8.00 and 16.00 ng/larva, mortalities were 9.25, 26.07, 50.32, 56.28 and 92.44%, respectively. The dose for 50% mortality (LD50) of methanol extracts by applied by a topical method with 1 µL of acetone solution was 1.92 ng/larva. Nutrient reserves like protein, glycogen and lipid are known to regulate pupation and adult emergence. These reserves have been found to be lower in treated larvae, indicating the insecticidal role of methanol extracts from G. biloba against third instar larvae of S. exigua.

발표요지 : 특별강연 ; 아카시아및 싸리배양세포로부터 원형질체의 분리및 융합

소상섭,여읍동,소웅영 한국식물생명공학회 ( 구 한국식물조직배양학회 ) 1986 한국식물생명공학회 춘계학술연구발표회 Vol.1986 No.-

아카시아 培養 Callus에서 原形質體 分離에 미치는 培地窒素의 影響

蘇祥燮,李朱洙 全北大學校 基礎科學硏究所 1988 基礎科學 Vol.11 No.1

The growth and protoplast-isolation from epicotyl induced callus of Robinia pseudoacacia L were investigated from B_5-plot containing nitrate and ammonium, NO_3-plot, NH_4-plot and nitrogen free(NF)-plot. Callus growth was the most in B_5-plot, and the least in NF-plot. For the isolation of protoplasts, 3 % cellulase, 1 % macerozyme, 13 % mannitol and 1 % sorbitol were used as the enzyme solution. The yield of protoplasts was the highest in the ammonium plot showing the maximum value of 7.0 ×10^5/gm. fresh weight from the callus after 15 days of the culture. And the others less than NH_4-plot in the yield of protoplasts, revealed the maximum values in the order of NF-, NO_3 -and B_5 plot. The incubation time for the highest yield of protoplasts from each was 3,4 5 and 6 hours for NH_4-, NF-, NO_3 -and B_5_plot, respectively. This result implies that the nitrate in the growht media lowers the isolation rate when a cultured callus is used for the protoplast-isolation.

담배 原形質體의 培養에 미치는 培地窒素 및 數種호르몬의 影響

蘇祥燮,申明淑 全北大學校 敎育大學院 1992 敎育論叢 Vol.12 No.-

Researching the effect of medium-nitrogen and hormones on the culture of protoplasts isolated from Mesophyll of Nicotiana tabacum L., protoplasts were cultured on the B5 media supplemented with the various concentration of inorganic nitrogen and hormones for 10 days. After measuring the viability and division rate of protoplast on the various experimental plots, multiple regression analysis was applied to experimental results. The regression constant value of viability is 62.623 and regression coefficient values of nitrate, BAP and incubation time are 0.005, -2.395 and -1.908 respectively. But the effects of ammonium, NAA and 2.4_D are not significant. The regression constant value of division rate is _2.276 and regression coefficient values of ammonium, nitrate and incubation time are 0.032, 0.002 and 1.190 respectively. But the effects of NAA, 2.4_D and BAP are not significant. The viability and division rate of protoplast were high percentage in the B_5 medium supplemented with ammonium 2mM, nitrate 50mM, BAP 1㎎/1 and NAA 3㎎/1. This result implies that ammonium and nitrate are essential components on the culture of tabacco protoplasts and plating efficiency will be increased when ammonium and nitrate concentration are increased in the B5 medium and when BAP concentration is appropriate.

아카시아 培養細胞에서 nitrate 및 ammonium 吸收에 미치는 無機燐酸鹽의 影響

蘇祥燮 全北大學校 1984 論文集 Vol.26 No.-

The effects of inorganic phsphate on absorption of nitrate and ammonium were determined in cultured acasia cells. The absorption in the PF-plot was similar to that of control plot for NH_4-N but slower for NO_3-N. The absorption in the PE-plot, on the other hand, was faster than that of control plot for both NO_3N and NH_4-N and the limiting concentration of phosphate was 3.0 mM in this case. However the cell growth in the PE-plot was slower than that of control plot, which revealed that the growth rate could not be directly related with the absorption of nitrogen and also it suggested that the uptake of the other nutrients were inhibited by the excessive phosphate. Therefore for the most effective growth the concentration of inorganic phosphate should be adjusted according to the level of the cultured cells or the other materials.

아카시아 배양세포의 생육 및 체내성분 변화에 미치는 무기태 질소의 영향

蘇祥燮 全北大學校 師範大學 1982 사대논문집 Vol.8 No.-

In cultured acasia cells, the effects of the inorganic nitrogen and the growth rates were determined from nitrogen and nitrogen free plot. Growth was largely influenced by the nitrogen added. Most of the added nitrogen sources were absorbed already by the 3rd day of culture. This phenomenon was more drastic in the case of ammonium. Growth rates showed a gain of up to 200% of the initial value in nitrogen plot. Free amino acids extracted from the cells grown in nitrogen plot represented a total of 15 amino acids. These were basic amino acids such as arginine, amides such as asparagine and neutral amino acids including leucine etc. On the other hand, 14 amino acids were extracted from nitrogen free plot-cells, while proline and threonine were not extracted in the latter stages of growth. The total amounts and the kinds of free amino acids released into the medium were less than those extracted from the cells.

느티나무(Zelkova serrata)충영에 관한 식물생리학적 연구

소상섭,김순영,오인숙 全北大學校 科學敎育硏究所 2000 과학과 과학교육 논문지 Vol.25 No.-

The relations between the galls and aphids which induce them on leaves of elm (Zelkova serrate) were investigated in the of botanical viewpoint. Gall formation begin on April 20s and matured completely on May 30s. Most of positions of galls induced on leaf were distributed around leaf-vein. These results also indicated that necessary nutrients for growth of galls were supplied by host plants. The growth of galls such as the length the width increased in proportion to life-cycle of fundatrix of emigant, and the stage of complete growthtime of galls was the same as that stage of alates. Tannin contents of galls were higher than those of host plant-leaf, and its tannin contents increased according to the growth of galls, showing the contents of tannin in the stage of complete growthtime were three times higher than in the early stage. The detection and change indole acetic acid in galls and aphids were confirmed in aphids but unconfirmed in galls during early gall-formation. However, those were confirmed in galls but unconfirmed in aphids during the maturing stage. These results may induce that IAA is a kind of gall formation-substance.

전계에 의한 무우종자 유식물의 유리아미노산 조성

소상섭,박안순,오인숙 全北大學校 科學敎育硏究所 2000 과학과 과학교육 논문지 Vol.25 No.-

According to the size of electrical treetment, the seed of Raphanus Sativus L. were seeded and then its germination rate and growth condition were checked and surveyed and at the same time, germination seedling was separated into stem or root. The composition and the contained quantity of free amino acids were measured and compared on the condition of each growing period. The rate of seed germination under the influence of the 2 voltage electrical field, which was 95 %, was the highest of all the testings, and the rate under 10 voltage was 3 % less germinated than that of normal control. The growth of seedling through all the organizations of stems and roots was the most effective and through all testings, the growth of root was better than that of stem. Through all testings, all kinds of the detected free amino acids were between 16 and 19. Especially, the testing under 2 volt hastened normal Nitrogen metabolism with the large amount of glutamine, asparagine and lysine showing up. And it produced the highest amount through all the testings. On the other hand, the testing under 5 voltage resulted in the one where the growth, the production of free amino acids and the amount were irregular. Therefore, it was certain that the limit of the most effective electrical field in this tested plant was under the influence of 2 voltage.

붉나무충영의 배양조직에서 생육 및 tannin 축적에 미치는 indole-3-acetic acid 의 역할

소상섭(Sang Sup So),허명자(Ming Zi Xu),오인숙(In Suk Oh) 한국환경생물학회 2001 환경생물 : 환경생물학회지 Vol.19 No.4

N/A These studies were carried out to examine the developmental process of galls caused by aphids in Rhus javanica and the effects of indole-3-acetic acid(IAA) and α-naphthalene acetic acid(NAA) on the tannin accumulation in the callus induced from that galls. The results are follows. The development of fist-shaped galls has begun at the beginning of June earlier than the case of finger-shaped galls, and also, the growth of fist-shaped galls has lost to September longer than the period of finger-shaped galls. These results indicate that the life cycle and feeding activity of the aphids inhabited in fist-shaped galls were longer and more active than the case of the aphids inhabited in finger-shaped galls. Tannin contents of fist-shaped galls revealed about 60∼70% of total dry weights during the whole growth periods, however, the contents in finger-shaped galls were under 10% at the maximum value. These facts mean that finger-shaped galls seem to be as a habitat of aphids rather than as a major source of tannin such as fist-shaped galls. The growht of callus induced from fist-shaped galls was the most effective in the plot of 10^-5 mole IAA, but the tannin accumulation in callus growth was not even caused in any plots of IAA treatments as wells as in any NAA plots. Thses results considered that the tannin accumulation in fist-shaped galls may be caused only in specific relation between host plant and life cycle of aphids.

느티나무에서 면충에 의한 충영형성과 아미노산종과의 연관성

소상섭(Sang Sup So),엄미정(Mi Jeong Uhm),오인숙(In Suk Oh),김환규(Hwan Gyu Kim) 한국환경생물학회 2001 환경생물 : 환경생물학회지 Vol.19 No.4

N/A After investigating the developmental process of galls forming on leaf of Zelkova serrata and analyzing the composition and contents of the free amino acids in leaves, galls and haemolymph of aphids, we have come to the following results. Galls induced were mostly distributed on the middle or lower part of leaf to the petiole along vein and most of investigated leaves had one gall only. The growth of galls continued in length and width until Paracolopha morrisoni habitants which transformed into alate adult left the gall. These results also indicated that galls are closely connected with a portion of plant tissues as well as foods and habitats of aphids. 26∼29 kinds of free amino acids such as proline, alanine and valine and so on, detected in leaves, gall and haemolymph of aphids. The composition of free amino acids were similar in three materials examined, but proline and asparatic acid showed the highest quantity in the leaves and galls, respectively. In case of aphids, alanine was extremely higher as 18.4% of total contents of free amino acids, in 1st instar of fundatrix stage. Total contents of free amino acids in the apteral adult stage of aphid were also increased than in the 1st instar stage. This investigation implies that nitrogen source such as free amino acids is apparently a major growth-material like carbohydrates during development of galls in Zelkova serrata.