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성영준,홍민의,장원석,심상준 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.0
Herein, we propose a novel approach to further facilitate the internal starch synthesis of C. reinhardtii through the operation of carbon-concentrating mechanism along with a two-stage process based on sulfur deprivation, thereby resulting in enhanced anaerobic capacity during PSII-dependent H2 photoproduction phase. When CCM-induced cells were exposed to high levels of CO2 with S deprivation internal levels of starch was significantly elevated by retaining a functional CCM with the boosted photosynthetic activity during 24 of S deprivation. As a result, during H2 production phase (III) at irradiance of 50 μE m(-2) s(-1), the concentrations of starch and hydrogen in CCM-induced cells were remarkably enhanced by 61.4% and 218.9% compared to that of CCM-uninduced cells, respectively. The treatment of low-CO2-driven CCM induction prior to S deprivation is a low-cost and energy strategy that improves the autotrophic H2 photoproduction by microalgae.
Photoautotrophic growth of microalgal cells in microdroplets using microfluidics
성영준,김영환,심상준 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.0
Microalgae have been considered as attractive source for carbon capture, utilization and storage (CCUS) because of their rapid growth and high photosynthetic efficiency compared to other biomass. Also, microdroplet system using two immiscible phases on PDMS based microfluidic system has been known as efficient analysis tool for cell kinetics. The microdroplets are regarded as an individual microreactor so it is possible to analyze a number of the single cell in parallel. By using these two systems, we can analyze the characteristics of microalgae efficiently. In this context, we encapsulated single microalgal cell into the microdroplets and reinjected the microdroplets into incubation chamber which consists of micropillar array. During the incubation of microalgal cell, we used humidifier to maintain the aqueous microdroplet intact. Therefore, we can monitor photoautotrophic growth of single microalgal cell in the various conditions related to CO2 concentration and light intensity.
Photoautotrophic cultivation of microalgae in microdroplet photobioreactor using microfluidic system
성영준,심상준 한국공업화학회 2018 한국공업화학회 연구논문 초록집 Vol.2018 No.0
In terms of CCUS technologies, microalgae have been an important role in a biological conversion of CO<sub>2</sub>. Microalgae have many advantages such as high photosynthetic efficiency and growth rate compared to another source of biomass. Microdroplet in microfluidics utilizing two immiscible aqueous and oil phases has been considered as an optimized analysis platform for analysis of cell kinetics. The microdroplets can be used as a photobioreactor so it is possible to analyze the growth kinetics of cells in single cell level. Thus, we could analyze the characteristics of microalgae efficiently. We encapsulated microalgal cells into the microdroplets and reinjected the microdroplets into another microfluidic chamber containing micropillar arrays. Finally, we could analyze the photoautotrophic growth of microalgal cells under various conditions such as CO<sub>2</sub> concentrations and light intensities.
성영준,노혜진,심상준 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.0
In terms of CCUS, microalgae play an important role in biological conversion of CO<sub>2</sub> and have advantages over other sources of biomass such as high photosynthetic efficiency and growth rate. Microdroplet in microfluidics utilizes two immiscible aqueous and oil phases and is considered as an optimized analytical platform, especially for singlecell kinetics. The microdroplets can be used as a photobioreactor so it is possible to analyze the growth kinetics of cells in single cell level. We encapsulated microalgal cells into the microdroplets and reinjected the microdroplets into another microfluidic chamber containing micropillar arrays for immobilization. We could efficiently analyze the photoautotrophic growth of microalgal cells under various conditions such as different CO<sub>2</sub> concentrations and light intensities. We could also simultaneously evaluate the photoautotrophic growth performance of four different microalgal strains in a short time.