마우스에 있어서 INAH의 면역독성에 미치는 Pyridoxine의 영향

선우연,김정훈,박찬봉,채병숙,강태욱,안영근 원광대학교 식품약품안전성연구소 1995 食品藥品安全性硏究 Vol.8 No.-

The immunopotenciating effect of pyridoxine on the immunotoxicity of INAH was investigated in ICR mice. ICR male mice were divided 4 groups(10mice/group) and pyridoxine and INAH were administered per oral for 28 days. ICR male were evaluated for changes in immune status as measured by antibody titer, Arthus reaction. delayed type hypersensitivity(DTH), rosette forming cell(RFC). and plaque forming cell(PFC) to sheep red blood cells(S-RBC). To investigate the change of the non-specific immune response, spleen cells of ICR male mice were assayed for natural killer(NK) cell activity against YAC-I lymphoma. Number of leukocytes in peripheral blood and phagocyte activity were measured also. The results were summarized as follows. 1. INAH decreased both humoral and cell-mediated immune responses. the weight ratios of spleen and thymus to body weight, NK cell activity, phagocyt activity, and number of leukocytes. 2. Generally, pyridoxine reduced or removed the decline of both humoral and cell- mediated of immune responses, NK cell activity, phagocyte activity, and number of leukocytes by immunotoxicity of INAH. The above results suggest that pyridoxine enhance both humoral and cell-mediated immune responses to in ICR mice, indicating that pyridoxine may block a immunoglobulin synthesis inhibition of INAH and pyridoxine deficiency.

食用油脂類에 對한 免疫生物學的 硏究

朴炳哲,文宰奎,朴榮吉,金杏順,安年衡,金度勳,金正勳,安榮根,李相根,鮮于演 圓光大學校 1990 論文集 Vol.24 No.2

食用油脂는 健康維持에 必須的인 營養素이다. 그러나 食用油脂는 脂肪을 構成하는 脂肪酸의 種類와 攝取하는 量에 따라 健康을 保衛하는 必須的인 食品의 成分이 되기도 하고 有害한 影響을 끼칠 수도 있어서 脂質代謝와 關連된 疾病 뿐만아니라 老化를 促進하고, 乳房癌, 大腸癌等의 癌發生과도 關連된 것으로 밝혀져 있다. 本 硏究는 우리生活環境에서 食用으로 接할 수 있는 10種의 油脂에 對한 免疫生物學的인 活性에서 究明한 結果 對照群인 참기름食餌群에 比해 올리브유食用群은 全般的으로 體液性 및 細胞性免疫을 有意性있게 增加시켰으나, 大食細胞의 活性과 末梢循環白血球數는 有意性없는 減少를 보였고, 動物性 油脂인 牛脂食餌群과 植物性 油脂인 옥수수기름食餌群, 들기름食餌群, 米糠油食餌群, 大豆油食餌群, 菜種油食餌群 및 고추씨기름食餌群에서는 全般的으로 體液性 및 細胞性免疫, 大食細胞의 活性 및 末梢循環白血球數를 有意性있게 減少시켰다. This study was performed to investigate the effects of edible oils and fats on the immunobiological responses in ICR male mice. Ten groups of experimental diets, such as sesame oil diet, beef tallow diet, lard oil diet, olive oil diet, corn oil diet, perilla oil diet, rice polishing oil diet, soybeen oil diet, rape seed oil diet, and red pepper seed oil diet were fed adlibitum to the ICR male mice for 27 days. The results of this study were summarized as followings. 1. Both humoral and cellular immune responses, phagocyte activity, and number of leukocytes in sesame oil group were increased. However, the increasing rate of body weight and the weight retios of spleen and thymus to body were generally decreased. 2. Hemagglutination titer(HA) and rosette forming cell(RFC) of the beef tallow group and the lard group were significantly lower than the sesame oil group(P<0.01), but the lard group significantly increased Arthus reaction and delayed type hypersensitivity(DTH)(P<0.01). 3. Arthus reaction, DTH, and RFC of the olive oil group were significantly higher than the sesame oil group(P<0.01). 4. DTH of the corn oil group was significantly higher than the sesame oil group(P<0.01) whereas phagochyte activity was significantly lower(P<0.01). But no statistically significant differences were found between the two groups in the humoral immune response. 5. HA, PFC, DTH, RFC, phagocyte activity, and number of leukocytes of the perilla oil group and the rice polishing oil group were significantly lower than the sesame oil group(P<0.01). 6. Both humoral and cellular immune responses, phagocyte activity, and number of leukocytes of the soybeen oil group, the rape seed oil group, and the red pepper oil group were significantly lower than the sesame oil group(P<0.01).

기니픽과 마우스에서 CFC-101 ( 녹농균 백신 ) 의 항원성시험

선우연(Woo Yearn Sun),한형미(Hyung Mee Han),김현수(Hyun Su Kim),백남진(Nam Jin Baek),김달현(Dal Hyun Kim),이동억(Dong Eok Lee),정승태(Seung Tae Chung),김필선(Pil Sun Kim) 한국응용약물학회 1994 Biomolecules & Therapeutics(구 응용약물학회지) Vol.2 No.4

As a part of the safety evaluation of Pseudomonas vaccine(CFC-101), antigenicity tests were carried out in guinea pigs and mice. In active systemic anaphylaxis(ASA) test, guinea pigs showed no sign or only moderate sign(1/5) when sensitized and challenged with up to 200 ㎍/㎏. In homologous passive cutaneous anaphylaxis(PCA) test using guinea pigs, inoculation of CFC-101 alone did not produce CFC-101-specific antibody. When inoculated with 200 ㎍/㎏ plus adjuvant, challenge of 200 ㎍/㎏ produced PCA titer of 32(5/5) but challenge of 20 ㎍/㎏ did not produce CFC-101-specific antibody. In heterologous PCA test using mice, CFC-101-specific antibody was not detected when sensitized with CFC-101 alone. Some animals(3/12) showed positive PCA response when inoculated with 200 ㎍/㎏ plus alum. In passive hemagglutination (PHA) test, although no antibody was detected at 20 ㎍/㎏, inoculation of 200 ㎍/㎏ alone or with alum produced positive response in all animals. This result has already been predicted because CFC-101 is a vaccine developed for the purpose of immunization. From the above results, it can be concluded that there is no adverse antigenic potential up to 10 times clinical dose of 200 ㎍/㎏.

Methamphetamine 이 면역장기 및 항체생성능에 미치는 영향

선우연(Woo Yearn Sun),한형미(Hyung Mee Han),윤은이(Eun Yi Yoon),신전수(Jeon Soo Shin),박현애(Hyeon Ae Park),김미영(Mi Young Kim) 한국응용약물학회 1994 Biomolecules & Therapeutics(구 응용약물학회지) Vol.2 No.1

BALB/c mice were intraperitoneally injected with methamphetamine (5 mg/kg) to observe the effect of methamphetamine on the immune system. Body weights were decreased in both acutely treated group (twice for 2 weeks with 7 days interval) and subchronically treated group (daily injection for 14 days). The relative spleen weights and the numbers of splenocytes were unexpectedly increased (p<0.05) in acutely treated group, but subchronically treated group showed the trend of decrease without significance. But there was no significant effect on antibody formation to hen egg lysozyme which was immunized during the treatment of methamphetamine and on plaque forming cell number. The relative thymus weights of both groups were significantly decreased by the treatment of methamphetamine (acutely treated group, p<0.05; subchronically treated group, p<0.01). These results suggest that the effect of methamphetamine on the immune system may be caused by thymic dysfunction.

Methamphetamine 이 B16 악성 흑색종 세포 전이에 미치는 영향

선우연(Woo Yearn Sun),한형미(Hyung Mee Han),신전수(Jeon Soo Shin),박현애(Hyeon Ae Park),정승태(Seung Tae Chung),김필선(Pil Sun Kim),손경희(Kyung Hee Sohn) 한국응용약물학회 1995 Biomolecules & Therapeutics(구 응용약물학회지) Vol.3 No.4

The effect of methamphetamine on the pulmonary metastasis was investigated in C57BL/6 mice injected with B16 melanoma cells. B16 melanoma cells (2x10^5 cells) were injected intravenously into 5∼7 weeks old C57BL/6 mice. Mice were then treated intraperitoneally with methamphetamine either acutely (two times with one week interval) or subchronically (daily for 14 days). Degree of pulmonary metastasis was investigated and specific immunologic parameters such as natural killer cell cytotoxicity(NKCC), antibody-dependent cellular cytotoxicity(ADCC) and blastogenic responses of splenocytes were examined. Mice which had been subchronically treated with methamphetamine showed significant decreases in the number of pulmonary metastasis of B16 melanoma cells, NKCC and ADCC without a significant change in blastogenic responses. In the acutely-treated group, slight trends of decrease in the numbers of pulmonary metastasis, NKCC and ADCC were observed without statistical significances whereas there was a significant increase in blastogenic responses. The mechanism underlying the decrease in the degree of metastasis despite diminished NKCC and ADCC after methamphetamine treatment and the relationship between the degree of pulmonary metastasis and duration of methamphetamine treatment remain to be investigated.

2.4-Dichlorophenoxyacetic Acid의 毒性에 對한 Ethanol의 影響

安榮根,鮮于演,鄭鍾甲,金正勳 한국환경독성학회 1989 환경독성보건학회지 Vol.4 No.2

The effects of ethanol on the toxicity of 2, 4-Dichlorophenoxyacetic acid in ICR mice were examined. The results were summerized as follows; 1. The LD_(50) of 2.4-dichlorphenoxyacetic acid sodium injected intraperitoneally in mice was approximately 367 mg/kg. When the animals were administered with ethanol, 2ml/kg or 4ml/kg the LD_(50) of 2.4-D sodium were decreased to 338 mg/kg or 32 mg/kg, respectively. 2. In the acute experimental group, spontaneous motor activity in the ANIMEX system exhibited dose-dependent decrease in mice administered with 2.4-D sodium and ethanol. 3. However, in subacute experimental group, spontaneous motor activity in the ANIMEX system exhibited dose-dependent increase in mice treated with ethanol.

大韓藥典 收載醫藥品의 分析法 器機化에 關한 硏究 : 초산 메테놀론 및 초산 노르에치스테론의 分析法 器機化 The Analytical Methods of Metenolone Acetate and Norethisterone Acetate by HPLC

金榮培,鮮于演,金昌洙,李承訓,黃永植,金永玉,羅燾喆,金益洙 국립보건연구원 1989 국립보건원보 Vol.26 No.

酵素劑의 力價基準및 試驗法 統一案에 關한 硏究 (Ⅲ) : Lipase

金榮培,鮮于演,金昌洙,李承訓,金永玉,羅燾喆,黃永植,金信根 국립보건연구원 1989 국립보건원보 Vol.26 No.

2.4-Dichlorophenoxyacetic Acid의 독성에 대한 Ethanol의 영향

안영근,선우연,정종갑,김정훈 환경독성보건학회 1989 환경독성보건학회지 Vol.4 No.3

The effects of ethanol on the toxicity of 2, 4-Dichlorophenoxyacetic acid in ICR mice were examined. The results were summerized as follows; 1. The LD$\sub$50/ of 2.4-dichlorphenoxyacetic acid sodium injected intraperitoneally in mice was approximately 367 mg/kg. When the animals were administered with ethanol, 2 ml/kg or 4 ml/kg the LD$\sub$50/ of 2.4-D sodium were decreased to 338 mg/kg or 32 mg/kg, respectively. 2. In the acute experimental group, spontaneous motor activity in the ANIMEX system exhibited dose-dependent decrease in mice administered with 2.4-D sodium and ethanol. 3. However, in subacute experimental group, spontaneous motor activity in the ANIMEX system exhibited dose-dependent increase in mice treated with ethanol.

수은이 시험관내 사람 다형핵백혈구의 기능에 미치는 영향

김효정,한형미,김순한,김옥연,선우연,윤은이 한국응용약물학회 1993 Biomolecules & Therapeutics(구 응용약물학회지) Vol.1 No.2

In the present study, the effect of HgCl₂ on the function of human peripheral polymorphonuclear leukocytes(PMNs) was examined. PMNs were isolated from human peripheral blood with density centrifugation in Ficoll-Paque. The cells were then incubated with 0.5∼5 μM HgCl₂ and glass adherence, chemotactic activity and erythrocyte-antibody rosette forming activity were measured. HgCl₂ decreased the function of PMNs in all three aspects tested. HgCl₂ significantly diminished glass adherence(0.5 μM: 92±12% (percentage of control, mean±S.D.); 1μM: 46±11%, P<0.01; 3 μM: 35±7%, P<0.01; 5 μM: 49±10%, P<0.01). Similarly, significant differences were observed in chemotactic activity after HgCl₂ treatment compared with control (control: 0.95±0.14 mm; 0.5μM: 0.91±0.11 mm; 1μM: 0.77±0.16 mm, P<0.05; 3μM: 0.61±0.06 mm, P<0.01; 5μM: 0.15±0.03 mm, P<0.01). Also, HgCl₂ decreased the percentage of rosette-forming PMNs, indicating diminished phagocytic activity of PMNs upon HgCl₂ exposure compared with control (control: 58±4%; 1 μM: 53±4%, P<0.05; 3 μM: 49±3%, P<0.01; 5 μM: 46±3%, P<0.01). Cell viability was not altered after HgCl₂ treatment (83±5% viability in control PMNs versus 81±8% viability in 5 μM HgCl₂-treated PMNs), suggesting that the impaired PMN function after HgCl₂ treatment was not due to nonspecific cytotoxicity induced by HgCl₂. HgCl₂-induced decrease in the function of PMNs may have some implications in depressed host susceptibilityupon bacterial challenge after mercury exposure.