샘창자막힘을 동반한 전내장역위증 신생아 1예

서요셉,김예정,호요한 대한주산의학회 2022 Perinatology Vol.33 No.1

Situs inversus totalis is a mirror reversal of the normal asymmetrical arrangement of the viscera. The incidence is approximately 0.3 in 10,000 live births. Situs inversus totalis is commonly seen in a condition known as primary ciliary dyskinesia, and can be associated rarely with various congenital abnormalities, such as polysplenia syndrome, biliary atresia, congenital heart disease, and midgut malrotation. The association of neonatal intestinal obstruction with situs inversus totalis, especially duodenal obstruction, is extremely rare with only few cases reported in the literature to date. We report one case of situs inversus totalis with duodenal obstruction and treated successfully by gastro-duodenostomy without complications.

Genetic Structure and Diversity of the Moon Jellyfish Aurelia coerulea Polyp Population in Jaran Bay, Korea, Revealed by Mitochondrial COI and 16S rRNA Genes

서요셉,Buhari Lawan Muhammad,채진호,기장서 한국해양과학기술원 2021 Ocean science journal Vol.56 No.1

Blooms of the moon jellyfish (Aurelia coerulea) have been responsible for huge economic losses and environmental disruptions in oceans around the world. The mass occurrence of these jellyfish may be highly related to the benthic polyp populations; however, studies supporting this are scarce. Here, we analyzed the genetic diversity and structure of Korean A. coerulea, using two mitochondrial genes, COI and 16S rRNA, in 51 polyps collected from Jaran Bay (South Korea) that is greatly affected by the mass occurrence of the jellyfish. Genetic diversity analysis identified 20 and 9 haplotypes in COI and 16S rRNA genes, respectively. TCS network analysis and haplotype distance matrix revealed a significant genetic structure in both genes. In addition, two distinct haplogroups (A and B) coexisted in Jaran Bay, and a single haplotype (COI-H4 or 16S-H2) dominated. These findings suggest a strong population differentiation among the polyp population that may have an effect on the jellyfish blooms in Jaran Bay.

병원사목의 새로운 방향

서요셉,Seo, Yo- 한국가톨릭의료협회 1978 한국가톨릭병원협회지 Vol.9 No.2

커튼원양해파리 Chrysaora pacifica (Goette, 1886) (Semaeostomeae; Pelagiidae)의 분자 마커를 이용한 한국내 지리적 분포

서요셉,김대현,채진호,기장서 한국해양과학기술원 2020 Ocean and Polar Research Vol.42 No.3

The distribution and genotypes of the sea nettle Chrysaora pacifica have been reported in the South Sea of Korea; however, little research work has been attempted in the East Sea. Here, we collected similar jellyfishes from the East Sea coasts (Goseong, Yangyang and Sokcho), and identified them to the sea nettle morphologically. In addition, the genotypes of these sea nettle were compared with those from the South Sea (Tongyeong and Geoje). Phylogenetic analysis by using the mitochondrial COI sequences showed that the genus Chrysaora was clearly separated from other taxa to be formed a monophyletic group, with each species distinctly separated. C. pacifica in the East and South Seas was separated geographically by the COI phylogeography, representing potentially different populations. The COI gene of the Korean C. pacifica had approximately 7 times more genetic variation than the nuclear ITS rDNA, and thus it might be considered as a useful marker for genetic analysis of the jellyfish population.

한국 보름달물해파리 Aurelia coerulea (Cnidaria; Schypozoa) 폴립 집단의 유전적 다양성 분석

서요셉,부하리 라완 무함마드,이연수,기장서 한국환경생물학회 2020 한국환경생물학회 학술대회 Vol.2020 No.07

Recent Trends in Biosensors Based on Electrochemical and Optical Techniques for Cyanobacterial Neurotoxin Detection

박정아,서요셉,손희상,박철환,민준홍,이택 한국바이오칩학회 2022 BioChip Journal Vol.16 No.2

Cyanobacteria that cause algal blooms are distributed worldwide and have been reported for a long time. Further, cyanobacterial growth is increasingly spreading because of anthropogenic eutrophication; this results in increasing damages by the production of a neurotoxin called cyanotoxin. Neurotoxins have adverse effects in humans, including disrupting signal transmission between neurons and muscles in various ways resulting in paralysis and death within minutes. To detect neurotoxins, analytical techniques such as high-performance liquid chromatography (HPLC), liquid chromatography–mass spectrometry (LC–MS), and enzyme-linked immunosorbent assay (ELISA) are being implemented, but have limitations as they are difficult to measure quickly in the field and are difficult to miniaturize. Introduction of biosensors for toxin detection provides a new direction as applying bioreceptors such as aptamers, antibodies, and nanomaterials can be used to create various sensors, without the limitations of conventional detection methods. In this review, we discuss the continued development of neurotoxin biosensors that are based on electrochemistry and optics.

기중성 남세균, Wilmottia murrayi (Oscillatoriales, Cyanobacteria)의 국내 미기록속 및 미기록종에 대한 연구

이남주,서요셉,기장서,이옥민 한국환경생물학회 2019 환경생물 : 환경생물학회지 Vol.37 No.3

Two aerophytic cyanobacteria from the rockwall of Haje port located in Geum river, Korea, were isolated in unialgal cultures and submitted to polyphasic evaluation. The filaments of the populations presented solitary or several to many parallel arranged. The straight trichomes were not attenuated with rounded apical cell. Phylogenetic analyses based on 16S rDNA sequences indicated that these populations formed the same clade with Wilmottia murrayi and had 99% or greater DNA similarity. Through the ultrastructure of the TEM, these populations showed parietal thylakoid arrangement, which coincides with family Coleofasciculaceae. From the above results, we reported the newly recorded genus Wilmottia, and species W. murrayi in Korea. 국내 금강수계의 하구역에 위치한 하제항 암벽으로부터 기중성 남세균이 채집되어, 단조류 배양되었다. 2개의 남세균 배양주로부터 형태적 형질과 TEM에 의한 세포 미세구조 특징을 관찰하였다. 또한 16S rDNA 염기서열을 규명하여 BLAST 검색, 염기서열 유사도, 유전거리 및 계통분석을 실시하였다. 국내 배양주는 Wilmottia murrayi와 계통학적으로 동일한 clade를 형성하였고, 99% 이상의 유사 도를 나타냈다. TEM을 통한 세포 미세구조는 틸라코이드가 여러겹으로 세포막 주변에 분포하는 특징을 나타냄으로써 Coleofasciculaceae과의 특징과 일치하였다. 이상의 결과를 통해 W. murrayi를 국내 미기록속 및 미기록종으로 보고하였다.

담수 남세균, Microcystis protocystis (Chroococcales, Cyanobacteria)의 국내 미기록종에 대한 연구

이남주,서요셉,기장서,이옥민 한국미생물학회 2020 미생물학회지 Vol.56 No.1

Microcystis protocystis was sampled from the Nakdong River, Geum River, and freshwaters of Yongin and Incheon. Within 3 days after sampling, morphologies of colonies and mucilage were observed using India ink. The colony of M. protocystis has irregular outlines and lacks distinct lobes and holes. Cells are distributed sparsely and irregularly within indistinct and homogeneous slime, which is diffuse at the margin. Three unispecific cultured strains from the So-ok stream were classified as Microcystis by phylogenetic analysis using 16S rRNA sequences. Three Korean isolates of M. protocystis showed 16S rRNA sequences completely identical to each other. The phylogenetic tree of the 16S rRNA sequences showed that Korean M. protocystis formed narrow clusters with other Microcystis members and M. protocystis with 99% or greater similarities. Additional trees of RNA polymerase beta-subunit gene (rpoB) sequences separated the Korean M. protocystis from other Microcystis. Through these results, M. protocystis was reported as a new species in Korea. 국내 낙동강 수계, 금강수계, 경기도 용인 그리고 인천광역시의 담수역으로부터 Microcystis protocystis를 채집하였다. 채집 후 3일 이내의 시료에 먹물을 사용하여 군체 및 점액질의형태를 관찰하였다. M. protocystis는 군체의 점액질 초 윤곽의경계가 불분명하고, 세포가 점액질 속에서 분산되어 있는 특징을 나타냈다. 단일 배양된 금강수계 소옥천의 3주의 배양주는 모두 동일한 16S rRNA 염기서열을 나타냈고, 계통수 분석을 통해 Microcystis 속으로 분류되었다. 분자계통분석에서국내 배양주는 기존에 보고된 M. protocystis를 포함한 다른Microcystis 종과 99% 이상의 유사도로 같은 클러스터를 형성하였다. 추가적인 rpoB 유전자를 이용한 분석은 한국의 M. protocystis를 다른 종과 분리하였다. 이와 같은 결과를 통하여M. protocystis를 국내 미기록종으로 보고하였다.

Evaluation of the complete nuclear rDNA unit sequence of the jellyfish Cyanea nozakii Kishinouye (Scyphozoa: Semaeostomeae) for molecular discrimination

Buhari Lawan Muhammad,서요셉,기장서 한국통합생물학회 2021 Animal cells and systems Vol.25 No.6

The harmful jellyfish Cyanea nozakii Kishinouye has frequently occurred on Korean coasts, and its blooms have caused serious ecological and economic damages. DNA sequences of the C. nozakii for molecular detection and discrimination are relatively scarce. In this study, we determined the complete sequence of a single unit of tandemly repeated ribosomal DNA (rDNA) of the Korean C. nozakii and characterized the molecular features of the rDNA. The complete rDNA contained 8,003 bp (48.4% GC) with the same gene arrangement (18S, ITS1, 5.8S, ITS2, 28S, and IGS) to the typical eukaryotes. Dot plot analysis showed that the coding regions (18S, 5.8S, and 28S) were highly conserved, while the non-coding regions (ITS1, ITS2, and IGS) were more variable and parsimony-informative. The IGS contained a putative transcription termination signal (poly(T) tract) and four repeats of block minisatellites. Phylogenetic analyses using 18S and 28S rDNA revealed well-resolved relationships of C. nozakii within the order Semaeostomeae, separating it from other Cyanea species. The complete rDNA sequence provides various options for the selection of jellyfish taxonomic markers and may be useful for discriminating between species of C. nozakii and phylogeny reconstruction with close relatives.