들깨, 차조기의 재배형 및 잡초형 계통들의 종자발아 특성

김진아(Jin Ah Kim),사규진(Kyu Jin Sa),김은지(Eun Ji Kim),마경호(Kyoung Ho Ma),유창연(Chang Yeon Yu),이주경(Ju Kyong Lee) 한국육종학회 2011 한국육종학회지 Vol.43 No.4

본 연구는 우리나라에서 자생 및 재배되고 있는 들깨와 차조기의 재배형 및 잡초형 계통들에 대하여 종자발아에 대한 변이 양상을 구명하기 위해 재배형 들깨 102계통, 잡초형 들 깨 41계통 그리고 잡초형 차조기 19계통들에서 측정된 종자 발아율 및 발아세는 다음과 같다. 1. 재배형 들깨는 저온처리 조건에서 1%에서 99%의 발아율 을 나타내어 평균 73%±24를 나타내었으나, 비저온처리 조건에서는 0%에서 98%의 발아율을 나타내어 평균 45% ±26를 나타내었다. 잡초형 들깨는 저온처리 조건에서 0%에서 97%의 발아율을 나타내어 평균 41%±38를 나타내었으나, 비저온처리 조건에서는 0%에서 71%의 발아율을 나 타내어 평균 22%±26를 나타내었다. 잡초형 차조기는 저온처리 조건에서 0%에서 51%의 발아율을 나타내어 평균 9%±14를 나타내었으나, 비저온처리 조건에서는 0%에서 9%의 발아율을 나타내어 평균 1%±2를 나타내었다. 2. 재배형 들깨는 저온처리 조건에서 0%에서 97%의 발아세를 나타내어 평균 63%±28를 나타내었으나, 비저온처리 조건에서는 0%에서 98%의 발아세를 나타내어 평균 35% ±25를 나타내었다. 잡초형 들깨는 저온처리 조건에서 0%에서 93%의 발아세를 나타내어 평균 33%±36를 나타내었으나, 비저온처리 조건에서는 0%에서 62%의 발아세를 나 타내어 평균 17%±21를 나타내었다. 잡초형 차조기는 저온처리 조건에서 0%에서 38%의 발아세를 나타내어 평균 5%±10를 나타내었으나, 비저온처리 조건에서는 0%에서 3%의 발아세를 나타내어 평균 0%±1를 나타내었다. 3. 재배형 들깨의 경우 102계통들 중 50% 이상의 발아율을 나타낸 계통들은 저온처리 및 비저온처리 조건에서 각각 86계통(84.3%)과 45계통(44.1%)를 나타내었으며, 50% 이상의 발아세를 나타낸 계통은 저온처리 및 비저온처리 조건에서 각각 71계통(69.6%)과 31계통(30.4%)를 나타내 었으며, 이들 중에서 저온처리 조건에서 28계통들은 90% 이상의 발아율을, 그리고 13계통들은 90% 이상의 발아세를 나타내었다. 반면에 잡초형 들깨의 경우 41계통들 중 50% 이상의 발아율을 나타낸 계통들은 저온처리 및 비저 온처리 조건에서 각각 19계통(46.3%)과 9계통(22%)를 나타내었으며, 50% 이상의 발아세를 나타낸 계통은 저온처리 및 비저온처리 조건에서 각각 14계통(34.1%)과 5계통 (12.2%)를 나타내었으며, 이들 중에서 저온처리 조건에서 5계통들은 90% 이상의 발아율을, 그리고 1계통은 90% 이상의 발아세를 나타내었다. 한편 잡초형 차조기의 경우 19계통들 중 저온처리 조건에서 단지 1계통(5.3%)만 50% 이상의 발아율을 나타내었으며, 50% 이상의 발아세를 나타낸 계통은 전혀 없었고, 대부분의 계통들은 10% 이하의 발아율과 발아세를 각각 나타내었다. 이상과 같이 본 연구결과는 오늘날 우리나라에서 자생 및 재배되고 있는 들깨와 차조기 작물의 재배형 및 잡초형 계통들에 대하여 수확 직후 이들 작물에서 종자발아 특을 이해하는데 유용한 정보를 제공할 것으로 생각된다. To clarify the seed germination characteristics among cultivated Perilla crop and their weedy types in Korea, we studied the germination percent and germination energy of 162 accessions (102 cultivated var. frutescens, 41 weedy var. frutescens, and 19 weedy var. crispa) in both conditions of 4℃ low temperature treatment and non-cold treatment. In our study, most accessions of cultivated var. frutescens showed more than 50% in both germination percent and germination energy in both cold and non-cold treatment conditions. Whereas, most accessions of weedy var. frutescens and weedy var. crispa showed lower than 50% in both germination percent and germination energy in both cold and non-cold treatment. In addition, most accessions of Perilla crop and their weedy types showed much higher germination percent and germination energy in 4℃ low temperature treatment condition compared to the seeds under non-cold treatment condition. The information provided in this research may help for our understanding the variation of seed germination characteristics among accessions of cultivated Perilla crop and their weedy types in Korea.

Genetic Analysis of Accessions of Cultivated and Weedy Types of Korean Perilla Crop using Phenotypic Characteristics and SSR Molecular Markers

So Jung Jang(장소정),Kyu Jin Sa(사규진),Hyeon Park(박현),Ju Kyong Lee(이주경) 한국약용작물학회 2023 한국약용작물학회 학술대회논문집 Vol.2023 No.1

SSR 분자마커를 이용한 색소 및 비색소옥수수 핵심집단의 유전분석

김병완(Byeong Wan Kim),사규진(Kyu Jin Sa),박기진(Ki Jin Park),박종열(Jong Yeol Park),이주경(Ju Kyong Lee) 한국육종학회 2015 한국육종학회지 Vol.47 No.1

In this study, genetic diversity, genetic relationship, population structure and association analysis among 12 colored and non-colored maize lines were investigated using 300 simple sequence repeat (SSR) markers. In genetic diversity analysis, a total of 1,331 alleles were identified at all the loci with an average of 4.44 and a range between 2 and 10 alleles per locus. The major allele frequency varied from 0.25 to 0.92 with an average of 0.48. Among 1,331 alleles, 221 alleles were colored maize specific allele, and 408 alleles were non-colored maize specific allele. While 702 alleles were shared in colored and non-colored maize lines. In addition, colored maize specific alleles were detected in 163 SSR markers. From population structure analysis, the 12 colored and non-colored maize lines were separated with based on the membership probability threshold 0.8, and divided into groups I, II, III and admixed group. The results of this study could serve as useful molecular markers for identification and characterization of colored and non-colored maize lines in the maize breeding studies.

한국에서 수집한 재배형 들깨 계통들의 형태적 변이

김동민(Dong Min Kim),사규진(Kyu Jin Sa),이수경(Sookyeong Lee),이주경(Ju Kyong Lee) 한국육종학회 2021 한국육종학회지 Vol.53 No.3

To better understand the morphological variation in the 189 accessions of cultivated var. frutescens of Perilla crop preserved atthe RDA-Genebank in South Korea, morphological characteristics were investigated using seven traits related to leaf quality. In the principalcomponent analysis (PCA), the first and second principal components accounted for 28.9% and 19.7% of the total variance, respectively. In the first component, leaf color (QL1) and stem color (QL3) contributed positively, whereas the flowering period (QL5) and degree ofpubescence (QL6) contributed in a negative manner. In the second component, QL5 and QL6 contributed positively, whereas the fragranceof plants (QL7) contributed negatively. The results of PCA showed that the 189 Perilla accessions were clearly divided into three groupsbased on the 1st axis (X-axis), except for some accessions. However, the Perilla accessions collected in South Korea and abroad did notshow a clear geographical distribution between the collection areas. Correlation analysis among the seven traits of 189 Perilla accessionsshowed a positive correlation coefficient for the combination of QL1 and QL3, and QL5 and QL6, and a negative correlation for the combinationof color of the reverse side of the leaf and QL6, and QL5 and QL7. Therefore, the results of this study will provide useful informationfor the selection of useful resources for the development of leafy vegetable varieties of the Perilla crop and the preservation and managementof genetic resources of the Perilla crop preserved at the RDA-Genebank in South Korea.

Morphological Variation of Cultivated and Weedy Types in Perilla Crop Collected from South Korea

Zhen Yu Fu,Kyu Jin Sa(사규진),Soo Kyeong Lee(이수경),Ju Kyong Lee(이주경) 한국약용작물학회 2023 한국약용작물학회지 Vol.31 No.1

옥수수 유망 자식계통들에 대한 잡종강세 및 수량관련 형질의 유전

박종열(Jong Yeol Park),우수연(Su Yeon Woo),사규진(Kyu Jin Sa),이주경(Ju Kyong Lee) 한국육종학회 2015 한국육종학회지 Vol.47 No.4

Grain yield, agronomic traits, and correlation between genetic distance of 36 F1 hybrids produced by half-diallel crosses using nine maize inbred lines were analyzed. In the analyses of F1 hybrids and their mid-parent heterosis (MPH) for agronomic traits, grain yield showed highest MPH value of 156%. One-hundred kernel weight showed the lowest value of 7%. In addition, when genetic distance based on agronomic traits was estimated, parental inbred lines did not agree with their own pedigree. Therefore it had the limitation to estimate genetic distance using agronomic traits. In this study, 92 SSR markers were used to calculate genetic distance at DNA level. However we did not confirm their own pedigree of nine parental inbred lines. There was no correlation between grain yield and SSR markers. Therefore molecular marker selection was conducted in relation with grain yield by the step-by-step method using 92 SSR markers. The selected nine SSR markers showed a significant positive correlation (r2=0.703**) between grain yield and SSR markers. The correlations between the selected SSR markers and agronomic traits of ear length, ear width, plant height, and ear height were particularly showed positively correlated. The nine SSR markers selected in this study would help predicting heterosis and planning crosses for hybrids in maize breeding programs.

핵심집단 육성을 위한 옥수수 계통의 형태적 특성 연구

김오겸(O Gyeom Kim),홍탁기(Tak Ki Hong),고우리(Woo Ri Ko),우수연(Su Yeon Woo),마슐쥔(Shi Jun Ma),박종원(Jong Won Park),사규진(Kyu Jin Sa),이주경(Ju Kyong Lee) 한국육종학회 2016 한국육종학회지 Vol.48 No.2

In order to develop a core set and new corn variety in Korea, we evaluated the morphological characteristics of 194 maize accessions by examining eight quantitative characteristics. On the evaluation of quantitative traits for 194 maize accessions, they showed the morphological variations in tassel length (35.1±5.0 cm), plant height (226.1±33.7 cm), ear height (86.3±22.6cm), stem diameter (2.3±0.6 cm), leaf width (9.3±1.1 cm), ear length (14.5±2.4 cm), ear row number (14.1±1.9 row), and 100 kernel weight (24.9±4.4 g). The results of principal component analysis (PCA) indicated that the tassel length, plant height, and ear height greatly contributed to positive direction on the first principal component axis. One-hundred kernel weight contributed to negative direction on the second principal component axis. Thus these morphological characteristics, which contributed greatly in the first and second principal components, might be useful for discrimination among 194 maize accessions. In our study, seven accessions, such as IT026357, IT026441, IT027321, IT033271, IT033591, IT033597 and IT124273, particularly were measured high on yield-related traits. Consequently, the 194 maize accessions used in this study could be used as promising materials for maize breeding programs such as development of new hybrid in Korea.

국내육성 팝콘옥수수 팝핑부피 향상을 위한 적정 수확시기

최재근 ( Jae-keun Choi ),류시환 ( Si-hwan Ryu ),김희연 ( Hee Yeon Kim ),김문종 ( Moon-jong Kim ),한정헌 ( Jeong-heon Han ),왕승현 ( Seung Hyun Wang ),김기선 ( Ki Sun Kim ),사규진 ( Kyu Jin Sa ),이주경 ( Ju Kyong Lee ) 강원대학교 농업생명과학연구원 2022 강원 농업생명환경연구 Vol.34 No.0

국내육성 팝콘옥수수 팝핑율 향상을 위하여 ‘오륜팝콘’, ‘오륜2호’, ‘지팝콘’, ‘기찬팝콘’에 대해 출사 후 수확시기별 팝핑율을 비교하였다. 국내육성 팝콘옥수수의 생육특성은 출사일수는 78-81일 사이로 나타났으며, 착수고율은 53.4-64%로 다소 높게 나타났다. 출사 후 등숙 기간에 따른 이삭특성은 등숙 기간이 길수록 이삭장은 짧아지고 백립중은 증가하였다. 팝핑부피는 ‘오륜팝콘’과 ‘오륜2호’ 품종에서는 출사 후 40일 후 측정에서는 각각 29.8 ± 0.2 cm³/g, 30.4 ± 0.1 cm³/g로 낮게 나타났으나 70일 후 수확에서는 34.8 cm³/g, 35.8 ± 0.1 cm³/g로 높게 나타나 두 품종의 수확 적기는 출사 후 70일로 나타났다. ‘지팝콘’과 ‘기찬팝콘’은 출사 후 40일 28.6 cm³/g, 34.3 ± 0.1 cm³/g로 나타났으나 60일 후 팝콘부피가 35.2 ± 0.1 cm³/g, 35.9 ± 0.1 cm³/g 증가하여 최적 수확기는 출사 후 60일로 나타났다. 수확기에 따른 일반성분 분석에서는 등숙 기간이 길수록 수분함량이 감소하였으며, 탄수화물, 단백질 함량은 증가하였다. 조지방 함량은 3.0 g/100 g대로 나타났다. 따라서, 국내육성 팝콘옥수수의 수확시기는 출사 후 60-70일이 적정하며 팝콘의 팝핑부피 품질을 높일 수 있는 최적 수확시기라고 판단된다. In this study, we investigated the effects of harvest time on the popping volumes of “Oryun popcorn,” “Oryun #2,” “G popcorn,” and “Kichan Popcorn” to improve the popping quality of domestic maize. The silking days of the four popcorn maize varieties ranged from 78-81 d and their plant-ear height ratio was 53.4-64%, which is rather high. The ear lengths were shorter and the grain weights were greater at 70 days after silking (DAS) than at 40 DAS. For the “Oryun Popcorn” and “Oryun #2” varieties, the popping volumes measured at 40 DAS were 29.8 ± 0.2 cm³/g and 30.4 ± 0.1 cm³/g, respectively, and at 70 DAS were 34.8 ± 0.0 cm3/g and 35.8 ± 0.1 cm³/g, respectively. The ideal harvest time for both varieties was 70 DAS. The popping volumes of “G Popcorn” and “Kichan popcorn” varieties evaluated at 40 DAS was 28.6 cm³/g and 34.3 ± 0.1 cm³/g, respectively. The optimal harvest time for these varieties was 60 DAS, when their popping volumes were 35.2 ± 01 cm3/g and 35.9 ± 0.1 cm³/g, respectively. Analysis of the general composition of the harvest showed that the longer the ripening period, the lower the moisture content and the higher the carbohydrate and protein content. The crude fat content was less than 3.0 g/100 g. Therefore, the optimal harvest time for domestically grown popcorn maize that maximizes the popping volume for improved popcorn quality was 60-70 DAS.