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홑파래로부터 추출한 Rhamnan Sulfate의 항보체 활성
빈재훈,김현대,류병호 한국식품영양학회 1996 韓國食品營養學會誌 Vol.9 No.4
홑파래로부터 황산기를 함유한 다당체를 크로마토그래피로 분리정제하여 rhamnan sulfate가 항보체 활성화에 미치는 영향을 조사하였다. 항보체 활성능력은 F-4-3 획분을 비교군으로 Heparin H-180, Dextran과 비교해 결과 비교군보다 높았고, C4a와 C3a의 convertase의 형성과 기능을 F-4-3 획분이 억제하였다. 이러한 보체 활성화 양식은 classical pathway 및 alternative pathway로도 경유함을 알 수 있었다. The anti-compliment activity of hemolytic complementary assay(TCH_50) of rhamnan sulfate fraction obtained from water extracts of Monostroma nitidum was investigated Rhamnan sulfate Fraction, F-4-3 fraction appeared relatively strong anti-complementary activity which decreased TCH_50 over 60% than that comparison with control, and F-4-3 considerably inhibited ACH50 F-4-3 inhibited formation of the classical pathway C3 convertase or C4 cleavage. The results also indicatd the mode of complement activation by F-4-3 fraction shows not only the classical pathway but also the alternative pathway.
홑파래에서 추출한 Rhamnan sulfate 의 Macrophage 에 의한 면역조절효과에 관한 연구
빈재훈,김희숙,류병호 한국식품위생안전성학회 1996 한국식품위생안전성학회지 Vol.11 No.4
The rhamnan sulfate extracted from green algae seaweed, Monostroma nitidum was characterized as activity in vitro culture assay with macrophages from mice. Rhamnan sulfate indicated that F-4-3 fraction enhanced glucose consumption, as well as the production of nitrogen dioxide and tumor necrosis factor(TNF). F-4-3 fraction was also augmented IL-1 secretion from those macrophages. Effects of the pretreatment of peritoneal macrophages with rhamnan sulfate F-4-3 fraction and several polysaccharides as relative standard on the production of H₂O₂ induced with unopsonized zymosan A were examined. Pretreatment with polysaccharides inhibited the zymosan A mediated H₂O₂ production by macrophages. The phorbol myristate acetate (PMA) mediated H₂O₂production was not affected by the pretreament. These result suggested that pretreatment of rhamnan sulfate interfered with the interaction of macrophages zymosan A. Rhamnan sulfate inhibited zymosan A mediated production of H₂O₂ by macrophages and F-4-3 fraction was also activator of macrophages.
Chitosanase의 분해에 의한 Chitooligo당의 분리 정제
류병호,빈재훈,이성호 한국식품영양학회 1995 韓國食品營養學會誌 Vol.8 No.1
키토산을 효소로 분해하여 생리활성물질이 우수한 chitooligo당을 분리하여 정제를 시도하였다. Chitosan을 탈 아세틸화 시킨 후 탈 아세틸화도(DAC)도가 DAC-45%, DAC-70%, DAC-75% 및 DAC-99%를 기질로 하여 chitosanase(Bacillus pumilus BN-282)로 분해한 결과 DAC-99가 분해능이 가장 우수하였다. 따라서 DAC-90을 이용하여 chitosanase로 분해한 다음 gel filteration하여 Biol-2 gel chromatography로 분획한 획분을 HPLC로 분리 정제한 결과 chitooligo당의 조성은 GlcN, GlcN_2, GlcN_3, GlcN_5 및 GlcN_6임을 확인하였다. This studies were carried out to purification and separation of chitooligosacchariches which containing excellent biological active substance. After deacetylation of chitosan (DAC%), DAC-45%, DAC-70%, DAC-95% and DAC-99% were used substrates and hydrolyzed by chitosanase (Bacillus pumilus BN-262) DAC-99% has excellent hydrolyzate which contained several chitooligosaccharides. Therfore, chitosan was hydrolyzed DAC-90 as substrate by chitosanase, and then purified and seperated of chitooligosaccharides Gel filteration and HPLC. This oligosaccharides composed with GlcN_0, GlcN_2, GlcN_3, GlcN_5 and GlcN_6.
류병호,오윤근,배기철,빈재훈 한국농화학회 1989 Applied Biological Chemistry (Appl Biol Chem) Vol.32 No.3
Bacteria utilizing naphthalene as a sole carbon source for growth were isolated and identified and code named as Acinetobacter calcoaceticus R-88, Pseudomonas testosteroni R87 and Pseudomonas putida R-89. Among these isolates, A. calcoaceticus R-88 found most effective in utilizing naphthalene. The optimal pH, temperature and concentration of naphthalene was 7.0, 30℃ and 10mM, respectively. The strain degraded naphthalene to salicylic acid as an intermediate. And the strain showed to be resistant to ampicillin, tetracyclin, chloramphenicol and kanamycin. A. calcoaceticus R-88 harbored plasmid DN A which was believed to be involved in naphthalene degradation.