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제주재래마의 보존을 위한 혈청 단백질 및 효소의 유전적 다형현상
한상기(S . K . Han),정의룡(E . Y . Chung),신유철(Y . C . Shin),변희대(H . D . Byun) 한국축산학회 1995 한국축산학회지 Vol.37 No.1
Biochamical polymoiphisms of four serum proteins (Albumin(Alb), Vitamin D binding protein(Gc). Postalbumin(Pa) and Esterase(Es)) in Cheju native. horse were analyzed as genetic markers by electrophoresis and their phenotype, genotype and gene frequencies were estimated in order to determine the genetic structure of Cheju native horses. In the Albumin(Alb) locus, three different phenotypes FF, SS and FS were recognized and assumed to be controlled by two autosomal codominant alleles designated AIb^F and Alb^S. The observed distribution of phenotypes were 59.32% for FS type, 25.42% for SS type and 15.25% for FF type. Gene frequencies of Alb^F and Alb^S were 0.4491 and 0.5509, respectively. In the Vitamin D-binding protein(Gc) locus, two different phenotypes, FF and FS, were obsened and assumed to be controlled by two autosomal codominant alleles designated Gc^F and Gc^S whereas the SS type was not recognized. The frequencies of genotype FF, and SS, were found to be 92.19 and 7.81%, respectively and gene frequencies of Gc^F and Gc^S were 0.9609 and 0.0391. In the Post-albumin(Pa) locus, two different phenotypes, FF and FS, were identified, which were considered to be controlled by means of two codominant alleles and at a single autosomal locus, but the SS type was not recognized. The distribution of phenotypes were 98.44% for FF type, 1.04% for FS, and gene frequencies of Pa^F and Pa^S were 0.9948 and 0.00.52, respectivety. In the Esterase(Es) locus, four different phenotypes were identified and assumed to be controlled by alleles designated Es^F, Es^I, Es^S and Es^O. The observed distribution of phenotypes FI, II, IS, FS, FF, SS and 00 were 41.78, 30.18, 26.00, 18.00, 14.46, 5.60 and 1.43, respectively. Gene frequencies of Es^I, Es^F, Es^S and Es^Owre 0.4643, 0.3214, 0.2000 and 0.0143 respectively. In comparison of genetic distance and dendogram calculated from the gene frequencies, the most close relationship was obtained between the Cheju and Kiso horses and the lowest genetic similarity was obtained between the Cheju and Arabian horses.
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재래한우의 보존을 위한 혈청 및 혈구단백질의 유전적 다형현상
한상기(S . K . Han),윤희섭(H . S . Yoon),정의룡(E . Y . Chung),신유철(Y . C . Shin),변희대(H . D . Byun) 한국축산학회 1995 한국축산학회지 Vol.37 No.1
Biochemical polymorphisms of five red cell and semen proteins, Hemoglobin(Hb), Transferrin(Tf), Post-transferrin 2(Ptf2), Post-albumin(Pa) and Albumin(Alb) as genetic markers in Korean cattle were analyzed by Starch and Polyacryamide gel electrophoresis and their phenotypes, genotypes and gene frequencies were estimated in order to analysis the genetic constitution of Korean native cattle population. In the Hemoglobin(Hb) locus four different phenotypes AA, AB, BB and CH were observed and assumed to be controlled by four different alleles designated Hb^A, Hb^B, Hb^C and Hb^H, and the Hb^H type was rare variant of Korean native cattle. The observed distribution of phenotypes were 73.37% for AA type, 23.37% for AB type. 2.72% for BB type and 0.54%r for CH type. Gene frequencies of Hb^A, Hb^B, Hb^C and Hb^H were 0.8505, 0.1440, 0.0027 and 0.0027. Semen Transfetrin(Tf) locus, 11 different phenotypes AA, AD₁, AD₂, AE, AH, D₁D₁, D₁D₂, D₁E, D₂H, D₂D₂, D₂E, EE and EH type were identified, which considered to be controlled by codominant alleles TF,^A Tf^D, Tf^D, Tf^E and Tf^H at a single locus. The frequencies of Tf genotypes AD₁, D₁E, D₁D₂, D₂E, AA, AE, D₁D₂, AD₂, D₁D₁, EE, AH, D₂H and EH were found to be 16.30, 13.33, 11.85, 10.37, 9.69, 8.15, 7.41, 9.63, 5.93, 4.44, 1.48, 0.74 and 0.01%, respectively. Gene frequencies of TF^A, Tf^(D1) Tf^(D2) and Tf^H were 0.2741, 0.2704, 0,2333, 0.2074 and 0.0148, respectively. And TfH gene were newly identified in Korean native cattle. Considering Post-transterrin 2 locus, three different phenotypess FF, FS and SS were identified, which considers to he controlled by two alleles Ptf^F and Ptf^S at a single autosomal locus. The frequencies of Rf genotypes FS, FF and SS were found to be 51.06. 36.88 and 12.06%n, respectively and gene frequencies of Ptf^F and Ptf^S were 0.6241 and 0.3759. In the Postalbumin(Pa) locus, three different phenotypes FF, FS and SS type were observed to be genetically controllled by Pa^F and Pa^S gene. And genotypes frequencies FS. FF amd SS type were 48.65, 36.(H and 1_5.32%, respectively. The gene frequencies of Pa^F and Pa^S were 0.6036 and 0.3964. The Albumin(Alb) locus were observed to lack any individual variation. Therefore, this locus were defined to be monomorphic. In comparison of genetic distance and dendogram calculated from the gene frequencies, close relationship was obtained between the Japanese cattle and the Korean cattle.
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