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Cytogenetic Studies With Cyclamate and Related Compounds
백원영 漢陽大學校文理科大學 化學科 1970 化脈 Vol.- No.1
Cyclamate, Cyclohexylamine, N-hydroxycyclohexylamine, and dicyclohexylamine can induce chromosomal damage in human leukocyte cultures.
성선자극호르몬이 흰쥐 난소의 GnRH와 GnRH mRNA의 발현에 미치는 영향
백원영,정파진,박신근,김완영,이종학,김종화,김명옥,최완성,Paik, Won-Young,Chung, Pa-Jin,Park, Shin-Keun,Kim, Wan-Young,Lee, Jong-Hak,Kim, Jong-Hwa,Kim, Myeong-Ok,Choi, Wan-Sung 대한생식의학회 1994 Clinical and Experimental Reproductive Medicine Vol.21 No.1
Expression of gonadotropin releasing hormone(GnRH) has been described in the rat ovary. It remains, however, unkown whether GnRH is synthesized as a prohormone. Therefore, this study was performed to verify the expression of pro-GnRH by in situ hybridization and further to investigate the effect of gonadotropin on GnRH or GnRH mRNA in rat ovary by immunohistochemical and in situ hybridization techniques. Adult female Sprague-Dawely rats were used and the estrous cycle was synchronized by intraperitoneal injection of pregnant mare's serum gonadotropin(PMSG). Ovaries were fixed with 4% paraformaldehyde and embedded with G.C.T. compound and cut by cryostat. For immunohistochemistry, avidin-biotin peroxidase complex(ABS) method was employed and for in situ hybridization, $^{35}S$-end labeled oligonucleotide was used and followed by autoradiography. By in situ hybridization using GnRH oligomer and GAP(GnRH associated protein) oligomer, GnRH mRNA and GAP mRNA were co-localized in the fullicular cells, luteal cells, interstitial cells and theca cells. GnRH or GnRH mRNA signals in the ovary increased by human chorionic gonadotropin(hCG) injection. At the 3 and 6 hrs after hCG injection, the number of GnRH and GnRH mRNA containing cells increased rapidly and the density of GnRH and GnRH mRHA culminated at 9 hrs after heG injection. With the follicular development, the high expression of GnRH and GnRH mRNA was also observed within the follicles. After ovulation, the density of GnRH or GnRH mRNA decreased in the follicles but increased in the corpus lutea.
생쥐 난자의 초자화동결 시 Cytochalasin B가 Cytoskeleton에 미치는 영향
백원영,최원준,김세나,이종학,Paik, Wong-Young,Choi, Won-Jun,Kim, Se-Na,Lee, Jong-Hak 대한생식의학회 2002 Clinical and Experimental Reproductive Medicine Vol.29 No.4
Objective : The purpose of this study was to evaluate the effect of Cytochalasin B (CCB) on the cytoskeletal stability of mouse oocyte frozen by vitrification. Methods : Mouse oocytes retrieved from cycle stimulated by PMSG and hCG were treated by CCB and then vitrified in EFS-30. These oocytes were placed onto an EM grid and submerged immediately in liquid nitrogen. Thawing of the oocytes was carried out at room temperature for 5 seconds, then the EM grid was placed into 0.75 M, 0.5 M and 0.25 M sucrose at $37^{circ}C$ for 3 minutes, each. These oocytes were fixed in 4% formaldehyde for an hour and then washed in PPB for 15 minutes 3 times, then incubated in PPB containing anti-tubulin monoclonal antibody at $4^{circ}C$ overnight. And then, the oocytes were incubated with FITC-conjugated anti-mouse IgG and propidium iodide (PI) for 45 minutes. Pattern of microtubules and microfilaments of oocytes were evaluated with a confocal microscope. Results: The rate of oocytes containing normal microtubules and microfilaments was significantly decreased after vitrification. The rate of oocyte containing normal microtubules in CCB treated group was higher than those in non-treated group (53.7% vs. 48.9%), but the difference was not significant. The rate of oocyte containing normal microfilaments in CCB treated group was significantly higher than those in non-treated group (64.5% vs. 38.3%, p<0.05). Conclusion: Microfilaments stability could be improved by CCB treatment prior to vitrification. It is suggested that CCB treatment prior to vitrification improve stability of cytoskeleton and then increase success rate in IVF-ET program using vitrification and thawing oocyte.
진공흡인에 의한 초기임신중절술에 있어서 전동식과 수동식흡인기의 효용차
백원영(WY Paik),이경희(KH Lee),김승욱(SW Kim) 대한산부인과학회 1986 Obstetrics & Gynecology Science Vol.29 No.2
진공흡인에 의한 초기임신중절술에 있어서 전동식과 수동식흡인기의 효용차 Suction curettage has been shown to be an effective and safe method of performing induced abortion during the first trimester of pregnancy and the vacuum needed for the suction curettage will usually be provided by an electrically operated pump. Unfortunately electricity supply may not always be available in certain area and an alternative source of vacuum will be needed. This study was undertaken to compare the practicability and safety of using mechanical pump developed by the Battelle Foundation for the first trimester abortion and to compare the outcome with that of abortions performed by the conventional electrically operated pump. A total of 460 cases of first trimester pregnancy were recruited and randomly allocated to either the mechanical or electrically operated pump group. After performing abortion, the volume of aspirates, the duration of the procedure and the number of times the cannulae was introduced into the uterus were recorded and both the immediate and delayed complications were assessed. The mean duration of gestation was 8.6 weeks in the mechanical pump group and 8.7 weeks in the electrical pump group. The average duration of procedure from the start of dilatation was 6.1 minutes in the mechanical pump group and 5.6 minutes in the electrical pump group. The mean volume of aspirate in the mechanical pump group was 101.2ml and mean volume in the electrical pump group was 104.2ml. The incidence of complication in the mechanical pump group was 3.1% whereas the same in the electirical pump group was 3.5% and the nature of complication were similar in both groups. From the above findings, it is concluded that the mechenical pump is safe for use in performing first trimester abortion although it is not as convenient as the electrically operated pumps.
Dehydroepiandrosterone Sulfate의 자궁경부숙화효과에 관한 연구
백원영(WY Paik),김승욱(SW Kim) 대한산부인과학회 1983 Obstetrics & Gynecology Science Vol.26 No.12
In order to study the effect of dehydroepiandrosterone sulfate (DHEA-S) on cerv ical ripening, DHEA-S was given intravenously 3 times a week to 30 primiparous women at term with unfavorable cervical condition for delivery. AIso 30 primipa rous women were recruited to the control group. The condition of the uterine cervix was assessed by Bishops pelvic scoring. and the change in pelvic score was carefully recorded. The results of this study were as follows: l. There were no statistically significant differences in clinical characteristics such as maternal age, gestational weeks, and Bishop score before administration of DHEA-S. 2. The increase in Bishop score in primiparous women given DHEA-S was sipi ficantly greater than that in the control group on the 2nd, 7th. and l4th day after first injection (p<0.01). 3. The duration from the first injection of DHEA-S to deliyery was significantly shorter than that in the control group (p<0.01). 4. No significant changes were noted in laboratory findings between before and after injection of DHEA-S. 5. No significant differences in effect on neonates were observed betwreen DHEA-S treated group and the control group. 6. Side effects of any type were not obseryed in the mothers.
GnRH - receptor mRNA의 임신 시기별 발현 양상
백원영(Won Young Paik),신반철(Ban Cheol Shin),신정규(Jeong Gyu Shin),최원준(Won Jun Choi),이순애(Soon Ae Lee),이종학(Jong Hak Lee),최완성(Wan Sung Choi),김명옥(Myeong Ok Kim) 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.12
N/A Objective: Our previous study demonstrated that the placental GnRH and GnRH mRNA did not parallel the time course of hCG secretion, though it is thought to be one of the potential paracrine regulators of hCG secretion from the trophoblasts. The present study was designed to examine the potential variation in GnRH-receptor mRNA expression in the placenta, which may account for the GnRH-mediated action of hCG secretion during pregnancy. Methods: Human placentas in firt, second, and third trimester of normal pregnancy were obtained. These placentas were fixed with 4% paraformaldehyde and embedded in OCT compound, and sectioned by cryostat. For in situ hybridization, S labeled RNA probes were used and followed by autoradiography. Results: The GnRH-receptor mRNA signals were present in both cytotrophoblast and syncytiotrophoblast cell layers. Signal intensities varied with gestational ages and were abundant at 6-7 weeks, peaked at 9-12weeks, declined at 14 and 24 weeks, and were barely detectable at term. The present study demonstrates that GnRH-receptor mRNA exhibits changes paralleling the time course of hCG secretion during pregnancy Conclusion: These data provide mechanistic understanding that the paracrine/autocrine regulation of hCG secretion by placental GnRH is mediated through an increase followed by a decline in GnRH-receptor mRNA expression from the first trimester to term placenta.