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한우(韓牛)에 감염(感染)된 Theileria sergenti merozoite 의 미세구조(微細構造)
백병걸,김병수,이호일,Baek, Byeong-kirl,Kim, Byeong-su,Lee, Ho-il 대한수의학회 1990 大韓獸醫學會誌 Vol.30 No.4
The fine structure of the inoculated Theileria sergenti on the splenectomized Korean native cattle was observed to delineate the morphological. characteristics with transmission and scanning electron microscope. The cattle was inoculated with 1.5ml cryopreserved stabilate ($5.63{\times}10^6/{\mu}l$, PE 3%). At peak parasitemia (40%), infected blood was collected, washed and then T sergenti was observed. Scanning electron micrograph of the erythrocytes infected with T sergenti appeared various irregular from involving specific swelling, and abnormal projections like acantocyte, echinocyte and knizocyte. Transmission electron microscopic studies of T sergenti showed that piroplasm possess intracytoplasmic food vacuole, rhoptries and tubule. Merozoite, $0.6{\sim}1.81{\mu}m$ to $0.4{\sim}1.21{\mu}m$ in length, surrounded by 10~15nm thickness of pellicula. which is surrounded by a single unit membrane. Various size of veil which was observed in stroma of erythrocytes infected with T sergenti, located at the proximate part of the merozoite. The merozoite multiplied by means of binary fission so that two and more oval-like merozoites in the stroma of infected erythrocyte could be observed.
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銀杏葉의 家兎 皮膚 接觸性炎症 誘發 毒性物質에 關한 硏究
白秉杰,金秉洙,金善熙,安丙浚 충남대학교 약학대학 의약품개발연구소 1991 藥學論文集 Vol.7 No.-
Gingko biloba is one of the oldest trees on earth. It can be traced 300 million years or more into past. In recent years, this tree has become increasingly popular because of its longevity. Amount of comsumption of Gingko leaves in Korea is increased to produce drugs for blood circulating system's disorder. The workers who work at the company of product drug with Gingko leaves complain pruritus at belt position of west, however toxic substance from extracts of Gingko biloba was carried out to determine what kind of the toxic substance from extract could induce a contact dermatitis by means of histopathological study. Dried leaves of Korean G. biloba were exhaustively extracted with chloroform. The aqueous chloroform residue was extracted with hexane. After evaporation, the hexane extract was passed through a silica gel column chromatography, it was separated into 9 fractions by means of gradient solvent(benzene: acetone/ 98~93:2~7%). The 4th and 7th fraction were idendified as bilibol and gingkolic acid by silica gel thin layer chromatography and NMR spectrophotometer. The 4th and 7th fractions of Korean Gingko leaves have an irritant substances which can be induced ear swelling and increased thickness of stratum cornium of rabbit ear exposed with the fractions (3 time/3 day, 100μl of 50mg/ml ethyl alcohol). In the 4th fraction, the substance have induced the surface of ear to swell and mass of unknown cell like inflammatory cell were infiltrated within the stratum corneum, and the 7th fraction have the surface of ear to loss the hairs, and sebaceous gland and follicle of hair were infiltrated with inflammatory cells, and other fractions at all did not induced a certain contact dermatitis and also Deutschen drug of Gingko (named as Gerigeron) have induced the contact dermatitis such as dematitis of Korean Gingko extract.
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白秉杰,崔仁赫,金平吉 전북대학교 농업과학기술연구소 1985 農大論文集 Vol.16 No.-
In order to investlagte effects of Cyperus rotundus on the ovarian growth of rabbits, C. rotundus which grows in Korea was boiled in water. the extracts of C. rotundus was administrated orally from the 30 days old to the 90 days old rabbits. At the 60 days old and 90 days old, five number of rabbits were slaughted respectively. After laparatoming, the ovaries were immediately fixed in 10% formalin solution, embedded in paraffin, serially sectioned at 6u, and stained with H & E. The results are as follow: 1. At the 60 days old, the weight and length of ovary of rabbits administrated extracts of C. rotundus were 7.63±0.73mg and 7.02±0.45mm respectively. But in the control group, 7.85±0.65mg and 7.11±0.57mm. 2. At the 90 days old, the weight and length of ovary in the experimental group were 36.04±1.58mg and 9.68±l.04mm respectively but in the control group, 31.62±2.12mg and 9.12±l.12mm. The weight and length of ovary in the experimental group were significantly increased compared with those in the control group (P < 0.05). 3. At the 90 days old, several graffian follicles and corpus luteum in the ovary of 3 among 5 rabbits were observed. Typical follicular atresia and "Call Exner Body" were observed predominatly in the experimental group and also observed twin oocyte in the secondary follicle. Above results, C. rotundus in Korea is considered as harmful to herbivorous animals, if eat voraciously.
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銀杏葉의 家兎 皮膚 接觸性炎症 誘發 毒性物質에 關한 硏究
白秉杰,金秉洙,金善熙,安丙浚 충남대학교 암연구소 1991 癌共同硏究所 硏究誌 Vol.1 No.1
Gingko biloba is one of the oldest trees on earth. It can be traced 300 million years or more into past. In recent years, this tree has become increasingly popular because of its longevity. Amount of comsumption of Gingko leaves in Korea is increased to produce drugs for blood circulating system's disorder. The workers who work at the company of product drug with Gingko leaves complain pruritus at belt position of west, however toxic substance from extracts of Gingko biloba was carried out to determine what kind of the toxic substance from extract could induce a contact dermatitis by means of histopathological study. Dried leaves of Korean G. biloba were exhaustively extracted with chloroform. The aqueous chloroform residue was extracted with hexane. After evaporation, the hexane extract was passed through a silica gel column chromatography, it was separated into 9 fractions by means of gradient solvent (benzene: acetone/ 98~93 : 2~7%). The 4th and 7th fraction were idendified as bilibol and gingkolic acid by silica gel thin layer chromatography and NMR spectrophotometer. The 4th and 7th fractions of Korean Gingko leaves have an irritant substances which can be induced ear swelling and increased thickness of stratum cornium of rabbit ear exposed with the fractions (3 time/3 day, 100μl of 50mg/ml ethyl alcohol). In the 4th fraction, the substance have induced the surface of ear to swell and mass of unknown cell like inflammatory cell were infiltrated within the stratum corneum, and the 7th fraction have the surface of ear to loss the hairs, and sebaceous gland and follicle of hair were infiltrated with inflammatory cells, and other fractions at all did not induced a certain contact dermatitis and also Deutschen drug of Gingko (named as Gerigeron) have induced the contact dermatitis such as dematitis of Korean Gingko extract.
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