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      • KCI등재

        분열효모에서 spDbp5 유전자의 결실돌연변이 제조와 기능에 대한 연구

        배진아,조현진,윤진호,Bae, Jin-Ah,Cho, Hyun-Jin,Yoon, Jin-Ho 한국미생물학회 2008 미생물학회지 Vol.44 No.1

        mRNA의 핵에서 세포질로의 이동에 중요한 역할을 하는 발아효모 Saccharomyces cerevisiae의 DEAD-box RNA helicase인 DBP5 유전자와 유사한 분열효모 Schizosaccharomyces pombe의 유전자(spDbp5로 명명)의 결실돌연변이주(knockout mutant)를 제조하여 그 특성을 조사하였다. 이배체인 S. pombe 균주에 하나의 spDbp5 유전자만을 결실시킨 후 4분체분석(tetrad analysis)을 수행한 결과, 이 유전자가 결실된 반수체 균주는 생장하지 못했다. mRNA의 핵에서 세포질로의 이동에 있어서 spDbp5의 역할을 알아보기 위해, spDbp5의 발현이 티아민(thiamin)에 의해 억제되는 균주를 제작하여 in situ hybridization을 통해 세포 내의 $poly(A)^+$ RNA 분포를 살펴보았다. spDbp5 유전자의 발현이 억제되면, $poly(A)^+$ RNA가 핵 안에 축적되고세포질에서는 줄어들었다. 이와 같은 결과들은 spDbp5 유전자 역시 mRNA의 핵에서 세포질로의 이동에 매우 중요한 역할을 담당하고 있음을 시사한다. We constructed the null mutants of fission yeast Schizosaccharomyces pombe spDbp5 gene that is homologous to DEAD-box RNA helicase DBP5 in budding yeast Saccharomyces cerevisiae, which plays important roles in mRNA export out of nucleus. A null mutant in an $h^+/h^+$ diploid strain was constructed by replacing the spDbp5-coding region with an $ura4^+$ gene using one-step gene disruption method. Tetrad analysis showed that the spDbp5 is essential for vegetative growth. The haploid spDbp5 null mutants harboring pREP81X-spDbp5 plasmid showed extensive $poly(A)^+$ RNA accumulation in the nucleus and decrease in the cytoplasm after repression of spDbp5 expression. These results suggest that spDbp5 is also involved in mRNA export from the nucleus.

      • KCI등재

        토픽 모델링을 활용한 북미의 퀘벡문학 연구동향 분석

        배진아,이준구 국제언어인문학회 2022 인문언어 Vol.24 No.1

        In this study, topic modeling technique, which is one of big data analysis techniques using artificial intelligence, was applied to the investigation of the research trends of Quebec literature in North America. The data collection was done through the Web of science, and 421 Quebec literature-related papers published in North America over the last 20 years were collected. The data consisted of the titles, abstracts, and keywords of these papers, and LDA, an algorithm for topic modeling was used to analyze the data. According to the Word Cloud result, it was found that the genres of ‘novel’ and ‘poetry’ were the most studied. As a result of the LDA analysis, eight topics were created, and the topics were : ‘Quebec identity and immigrant litterature’, ‘Short story and essay’, ‘Translation and various cultures’, ‘Quebec novels and authors’, ‘Contemporary Quebec theatre and drama’, ‘Poetry’, ‘History of Quebec literature’, and ‘Quebec women's literature’. The results of this study are significant in that they attempted to analyze a vast amount of literature research papers by applying big data analysis techniques based on artificial intelligence, and are expected to serve as a stepping stone for similar studies in the future.

      • KCI등재

        실천력 강화를 위한 인공지능 윤리 교육 모델

        배진아,이정훈,조정원 대한산업경영학회 2022 산업융합연구 Vol.20 No.5

        인공지능 기술로 인한 사회·윤리적 문제 사례가 발생하면서 인공지능의 위험과 부작용에 대한 사회적 관심과 함께 인공지능 윤리가 주목받고 있다. 인공지능 윤리는 알고, 느끼는 것에 그치는 것이 아니라 행동과 실천으로 이루어져야 한 다. 이에 본 논문은 인공지능 윤리의 실천력을 강화하기 위한 인공지능 윤리 교육 모델을 제안하고자 한다. 인공지능 윤리 교육 모델은 선행 연구 분석을 통해 교육목표와 인공지능을 이용한 문제해결 프로세스를 도출하고, 실천력 강화를 위한 교수학습방법을 적용하였으며 기존에 제안된 인공지능 교육 모델과 비교 분석하여 그 차이를 도출하였다. 본 논문에서 제 안하는 인공지능 윤리 교육 모델은 컴퓨팅 사고력 함양과 인공지능 윤리의 실천력 강화를 목표로 한다. 이를 위해 인공지능 을 이용한 문제해결 프로세스를 6단계로 제안하고, 인공지능 특성을 반영한 인공지능 윤리요소를 도출하여 문제해결 프로 세스에 적용하였다. 또한, 인공지능 윤리 의식에 대한 사전·사후 평가와 과정 평가를 통해 인공지능 윤리 기준을 무의식적 으로 확인하게 하고, 학습자 중심의 교수학습방법을 적용하여 학습자의 윤리 실천을 습관화하도록 설계하였다. 본 연구를 통해 개발된 인공지능 윤리 교육 모델이 컴퓨팅 사고력을 함양하고, 인공지능 윤리가 실천으로 이어지는 인공지능 교육이 될 수 있을 것으로 기대한다. As cases of social and ethical problems caused by artificial intelligence technology have occurred, artificial intelligence ethics are drawing attention along with social interest in the risks and side effects of artificial intelligence. Artificial intelligence ethics should not just be known and felt, but should be actionable and practiced. Therefore, this study proposes an artificial intelligence ethics education model to strengthen the practical ability of artificial intelligence ethics. The artificial intelligence ethics education model derived educational goals and problem-solving processes using artificial intelligence through existing research analysis, applied teaching and learning methods to strengthen practical skills, and compared and analyzed the existing artificial intelligence education model. The artificial intelligence ethics education model proposed in this paper aims to cultivate computing thinking skills and strengthen the practical ability of artificial intelligence ethics. To this end, the problem-solving process using artificial intelligence was presented in six stages, and artificial intelligence ethical factors reflecting the characteristics of artificial intelligence were derived and applied to the problem-solving process. In addition, it was designed to unconsciously check the ethical standards of artificial intelligence through preand post-evaluation of artificial intelligence ethics and apply learner-centered education and learning methods to make learners' ethical practices a habit. The artificial intelligence ethics education model developed through this study is expected to be artificial intelligence education that leads to practice by developing computing thinking skills.

      • KCI등재

        방송 콘텐츠 수입의 진화와 포맷 거래

        배진아 한국방송공사 2011 방송 문화 연구 Vol.23 No.2

        The periods are divided and the aspects of each period are interpreted in order to understand the historical evolution process of imported TV content. The first period(~1980s) is explained from the cultural imperialism perspective, and the cultural pride and identity are recovered in the second period(during 1990s). In the third period(2000s~), the pluralism is realized with the changes in global media environment. The analysis on the format trade as an evolved mode of program distribution in the industrial perspective draws four attributes of format trade: format trade involves the trade of brand value and reputation, the format as a product is being globally standardized, the localization in format trade is caused by economic motivations, and the international flow of format trade is unequal. According to those attributes format trade is interpreted as the representation of media imperialism subject to the domination of transnational corporations. The insights of cultural imperialism are applied to format trade for the following reasons: the international flow of format is unequal, the Western transnational companies dominate the format flow, and as a result the homogeneous culture is formed even in the most regional content such as reality genre.

      • KCI우수등재
      • KCI등재

        공영방송의 다원성 책무: KBS 사례를 중심으로

        배진아 한국방송공사 2023 방송 문화 연구 Vol.35 No.1

        With the development of the media industry, popular high-quality programs are increasing, while the size of the public and discriminatory minority areas is relatively reduced, making the value of media pluralism more important. This study believes that the public broadcasting system should play an important role in realizing the value of pluralism, and examines the responsibility of the public broadcasting system, focusing on KBS. Based on existing discussions on the value of media pluralism as a public responsibility of public broadcasting and the EU's MPM model, ‘a model for evaluating pluralism accountability for Public Broadcasting’ is proposed. This model contains 12 pluralistic evaluation factors in four dimensions: institutions and norms, competitiveness, political independence, diversity and inclusion. Based on the proposed model, an experimental evaluation of KBS's practice of pluralism responsibility was attempted. As a result of the evaluation, it was confirmed that the systems and norms corresponding to the basic conditions for promoting pluralism were insufficient, and the financial structure was weak and in terms of competitiveness. In addition, it was analyzed that institutional independence, a prerequisite for political independence, was not achieved, and partisan divisions and conflicts within the organization, including the press office, were factors that hindered the performance of responsibilities. Finally, in terms of diversity and inclusion, the diversity of workers and producers, diversity of content, and media diversity education were not actively implemented. Based on these evaluations, implications related to KBS's practice of pluralistic responsibilities were proposed.

      • KCI등재후보
      • KCI등재

        익산시 경관계획 수립 및 실행방안 확보에 관한 연구 : 동지역을 중심으로

        김현숙,배진아 전북대학교 부설 휴양및경관계획연구소 2011 휴양및경관연구 (J East Asian Landscape Studies) Vol.5 No.1

        익산시는 KTX역세권, 국가식품클러스터, 고도지정 등에 의한 대규모 도시개발 사업으로 인해 도시경관의 급격한 변화가 예상되므로 종합적인 경관계획을 필요로 하고 있다. 본 연구는 첫째, 익산시에 내재되어 있는 경관자원을 도출하여 자원의 보존, 연계방안을 설정하고, 둘째, 시가지 환경개선과 지역특색을 강화하기 위한 종합적이고 바람직한 경관계획을수립하며, 셋째, 주민과 함께하는 경관계획 실행 방안을 제안하는데 목적이 있다.연구는 도농통합시 익산의 중심시가지인 15개 동지역을 대상으로 현장조사를 통해 경관자원을 도출하고 자원의 가치및 중요도를 펑가한 후, 계획의 방향을 설정하였다. 경관유형은 자연경관, 시가지경관, 가로 및 철도경관으로 구분하였다.자연경관에서는 배산, 탑천, 대간선 수로의 보존 및 녹지의 네트워크화 방안이 모색되었다. 주거지, 상업지, 공업지및 KTX 역세권에 대해서는 주요 지역에 대한 특정경관계획과 건축물 및 외부공간을 중심으로 한 디자인 가이드라인을설정하였다. 가로 및 철도경관에서는 하나로, 송학로의 선형공원화를 통해 녹지 네트워크를 강화하였으며 철도변 경관저해요소 제거방안을 제시하였다.계획의 실행은 경관사업과 병행하여 주거지 경관정비는 주민 주도형 경관협정을, 탑천, 폐선부지, 하나로의 공원화사업은용도지구 지정을, 배산, 역세권, 대학로는 지구단위계획을 수립함으로서 규제 및 인센티브가 상호 보완적으로 나타날수 있게 하였다. Iksan-si has got to set a comprehensive landscape planning as there is expected a rapid change in the cityscape by the large scaled urban development from its designation of KTX station area, national food cluster, and historic city. Therefore this study was conducted with the following purpose. First, it sets a plan to identify the scenic resources in Iksan-si so that they can be conserved and linked with each other. Second, it sets the comprehensive and desirable landscape planning to improve the urban environment and reinforce its characteristics. Third, it suggests a implementing method for the landscape planning that can be practiced with residents. For this purpose the landscape resources were identified through the field survey on the base of downtown areas built with 15 dongs in Iksan and their value and importance were assessed. And then the method of conservation and the direction of development were decided. Landscape type was divided into four as natural, urban, street, and railroad landscape. For the natural landscape there was investigation on Baesan, Tapcheon, conservation of main road waterway, network of green belts. For residence areas, commercial areas, industrial areas, and KTX station areas a design guideline was made on the base of buildings and outer space as well as a specific landscape planning on the main areas. For street and railroad landscape, green belts network was reinforced with the construction of linear park for Hana-ro and Sonhak-ro and also a plan was suggested to remove the landscape impediments around railroads. To implement the plan for landscape, both the methods of regulation and incentive were taken by offering the residence areas project to citizen-leading agreement, park construction of Tapcheon, abolished railroads, and Hana-ro to designation of zoning district, and Baesan, station areas, and Dahak-ro to establishment of district unit planning.

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