미생물 오염 개선을 위한 화장품 패키지 디자인의 기술적 접근에 관한 연구

배은혜 한양대학교 대학원 2016 국내박사

Polydimethylsiloxane (PDMS) 기반의 수분산 폴리우레탄 코팅제 합성 및 특성 연구

배은혜 부산대학교 대학원 2023 국내석사

여름철 북극항로의 개방으로 신규 극지 운항 선박 또는 극지 해양플랜트의 수요가 증가하고 있다. 그렇기에 기존의 케이블은 -40℃ 정도의 저온 특성을 필요로 하였으나 더 극한 환경에서 운항하는 선박이 늘어남에 따라 극지 운항 선박에 사용되는 케이블은 최대 -60℃ 수준에서 유연성을 유지하는 저온 특성이 요구되고 있다. 본 연구에서는 polyol로 polyether polyol type인 poly(tetramethylene ether glycol) (PTMG)와 기능성 polyol로 저온특성과 유연성, 내수성이 우수한 polydimethylsiloxane (PDMS)를 혼합하여 사용하였으며 isocyanate로 isophorone diisocyanate를 사용하여 수분산 폴리우레탄 코팅 수지 (SWPU)를 합성하였다. 그리고 필름을 제조하여 그 특성을 분석하였다. FT-IR과 XRD, PSA를 통해 SWPU의 기본적인 화학구조와 결정 구조, 그리고 입자 크기를 분석하였고 그 결과 합성이 잘 이루어졌음을 확인하였다. 또한 TGA, DSC, DMA 분석을 통해 PDMS의 함량이 증가함에 따라 열적 특성이 향상되었음을 확인하였고 SWPU-2에서 제일 낮은 Tg를 갖는 것을 알 수 있었다. UTM을 이용하여 기계적 특성을 평가하였고 SWPU-2에서 제일 높은 강도와 연신율을 갖는 것을 확인하였다. 접촉각을 통해 표면 특성을 분석하였고 증류수와 해수에서의 팽윤비를 분석하여 PDMS의 함량이 증가함에 따라 접촉각이 증가하고, 팽윤비는 감소하였음을 확인하였다. 수분산 폴리우레탄 (SWPU)의 특성 분석을 통해 PDMS가 우수한 저온특성, 유연성, 내수성을 부여할 수 있음을 나타내었다. 또한 인장강도와 신장율, DMA, DSC 분석을 고려하였을 때 PTMG와 PDMS의 몰비가 1.495 : 0.005인 SWPU-2에서 최적의 특성을 가진다고 할 수 있다. With the opening of the Northern Sea Route in summer, the demand for new polar vessels or polar offshore plants is increasing. Therefore, the existing cables required low-temperature characteristics of about -40°C, but as the number of ships operating in more extreme environments increases, cables used for polar-operated ships are required to have low-temperature characteristics that maintain flexibility at a maximum level of –60°C. In this study, poly(tetramethylene ether glycol) (PTMG), a polyether polyol type, and polydimethylsiloxane (PDMS), which have excellent low-temperature characteristics, flexibility, and water resistance, were used as a functional polyol. Then, waterborne polyurethane coating resin (SWPU) was synthesized using isophorone diisocyanate as isocyanate. Then, a film was prepared and its properties were analyzed. The basic chemical structure, crystal structure, and particle size of SWPU were analyzed through FT-IR, XRD, and PSA, and as a result, it was confirmed that the synthesis was successful. In addition, through TGA, DSC, and DMA analysis, it was confirmed that the thermal properties were improved as the content of PDMS increased, and it was found that SWPU-2 had the lowest Tg. The mechanical properties were evaluated using UTM, and it was confirmed that SWPU-2 had the highest strength and elongation. The surface properties were analyzed through the contact angle, and the swelling ratio in distilled water and seawater was analyzed to confirm that the contact angle increased and the swelling ratio decreased as the content of PDMS increased. Through characterization of waterborne polyurethane (SWPU), it was shown that PDMS can impart excellent low-temperature properties, flexibility, and water resistance. In addition, considering the tensile strength, elongation, DMA, and DSC analysis, SWPU-2 with a molar ratio of PTMG and PDMS of 1.495 : 0.005 shows the best characteristics.

현대국어 자음군 단순화의 지역별 분포 양상과 내적 변화 연구

배은혜 경북대학교 대학원 2018 국내석사

This study aims to shed light on the regional distribution of and internal changes in the simplification of the consonant clusters by examining how stem-final consonant clusters are realized in local language data. In order to achieve this, the realizations of the simplification of stem-final consonant clusters in Korean Dialect Sourcebook and Reports on Local Language Investigation, which have a temporal gap of about a generation, were compared and analyzed. Chapter 2 reviews the theoretical background that explains the phenomenon of consonant cluster simplification. The phonological motivations that underpin the consonantal selection when simplification of consonant clusters occurs were examined. Consonant cluster types were classified according to the characteristics seen in the realization of the consonant clusters; that is to say, the classificatory criterion was based on whether the consonants constituting the consonant clusters were coronal or not. When simplification of consonant clusters occurs, a consonant drops out if it is the subsequent consonant in the cluster as well as a coronal consonant. It has been observed indeed that in consonant clusters such as ‘ㄳ, ㅄ’ and ‘ㄽ, ㄵ, ㄾ’, which fall under the [−coronal]+[+coronal] type and the [+coronal]+[+coronal] type respectively, the subsequent coronal consonant systematically dropped out. There are cases when the subsequent consonant is not a coronal consonant, that is, consonant clusters of the [+coronal]+[−coronal] type, such as ‘ㄶ, ㄺ, ㄻ, ㄼ, ㄿ, ㅀ’. Among those, only the clusters ‘ㄺ, ㄻ, ㄼ, ㄿ’ were considered in this study. In this type of consonant clusters, the subsequent consonant was not dropped and could be realized instead because it was not a coronal consonant. In other words, since the preceding consonant was a coronal consonant, the preceding consonant was dropped and the subsequent consonant could thus be realized. However, these consonant clusters were divided into two categories according to realization patterns: (1) ‘ㄺ, ㄼ, ㄿ’, where any of the two consonants can be realized, and (2) ‘ㄻ’, which is realized as ‘ㅁ’. This study seeks to explain the basis underpinning this distinction in sonority so as to understand why ‘ㄻ’ is realized as ‘ㅁ’. Chapter 3 examines the realization patterns of consonant cluster simplifications based on the classification made in Chapter 2. Stem-final consonant clusters in nouns were divided as follows: (1) ‘ㄺ, ㄼ’, which consist of a coronal consonant followed by a non-coronal consonant; (2) ‘ㄳ, ㅄ’, which consist of a non-coronal consonant followed by a coronal consonant; (3) ‘ㄽ’, which consists of two coronal consonants. As for verbs, only the [+coronal]+[−coronal] clusters were examined since consonant clusters simplification cannot be realized in verbal stems independently. Based on the Reports on Local Language Investigation, the consonant cluster simplifications that occurred in the 2000s were quantitatively analyzed and their characteristics described. Then, these were compared to the realization patterns in Korean Dialect Sourcebook, which presents the simplifications of consonant cluster which occurred in the 1980s, so as to examine how the realization of consonant cluster simplifications changed over time. Section 3.1 focused on the stem-final consonant clusters ‘ㄺ/ㄼ’, ‘ㄳ/ㅄ’, and ‘ㄽ’ in nouns. In the case of nouns, it clearly appeared that there tended to be more stem restructurings, which occurred under the influence of consonant cluster simplification, than optional realization of consonant cluster simplifications. In the case of ‘ㄳ’ in particular, the restructuring varied according to words even though it was the same consonant cluster. Section 3.2 focused on the verbal consonant clusters ‘ㄺ, ㄼ, ㄿ’ which showed optional realizations. Stem-final consonant clusters ‘ㄺ’ in verbs appeared to be realized in various ways before ‘ㄱ’. This was understood to result from dissimilation and rely on regional characteristics. Section 3.3 analyzes overall the characteristics of consonant cluster simplification discussed in the previous sections. The characteristics of the consonant clusters according to their types, the characteristics regarding the realization of the consonant clusters according to word categories, and the regional characteristics of the realization patterns were analyzed from various perspectives. The present work is significant in that it examines quantitatively the realization patterns in order to provide an objective account of the various tendencies in consonant cluster simplification.

북한의 영웅상 형성 연구: 3차례 영웅대회를 중심으로

배은혜 경남대학교 북한대학원대학교 2025 국내석사

탈(脫)영웅화하고 있는 현대사회에서 왜 북한은 여전히 영웅정치를 고수하고 있는 것일까. 이러한 의문에서 시작된 본 연구는 북한사에 총 3차례 개최된 영웅대회에 초점을 맞춘다. 북한 당국이 어떠한 대내외적 환경 변화 속에서 영웅대회를 개최했는지, 북한사의 맥락 속에서 영웅대회 의미와 대회마다 제시된 영웅상, 영웅들을 통해 인민들에게 주문한 과업은 무엇이었는지 살펴본다. 대회마다 북한 당국이 의도한 소기의 성과를 거두었는지 평가하고 이것이 오늘날 북한에 어떠한 영향을 미쳤는지 고찰한다. 북한은 전쟁, 사회주의 진영의 몰락과 경제 위기, 고난의 행군 등 체제의 존망에 영향을 미쳤던 굵직한 대내외적 환경 변화를 맞아 국면 전환이 필요할 때, 영웅대회를 개최해 영웅상을 제시하고, 영웅들을 앞장세워 인민들이 수행해야 할 과업을 제시했다. 정전협정 체결 3주 만에 개최된 1953년 전국전투영웅대회에서는 전쟁을 승리로 이끈 전쟁영웅들의 노고를 치하함과 동시에, 전후 복구와 국가 건설이라는 새로운 목표가 제시되었다. 경제 위기 속에서 사회주의 진영에 몰락의 그림자가 서서히 드리우던 1988년 개최된 전국영웅대회에서는 지난 날 천리마의 기세로 달려온 경제영웅들이 호명되었고, 이제 막 마무리된 200일 전투를 2,000일, 20,000일 전투로 이어나가줄 것이 요구되었다. 세계사에 ‘류례 없는’ 시련으로 남겨진 고난의 행군을 지나 2003년 새 세기에 개최된 선군시대영웅대회에서는 어떠한 고난과 시련에도 공화국과 수령을 배신하지 않고 결사옹위하는 사상영웅들이 등장했고, 인민들에게 따라 배울 모범으로 제시되었다. 북한의 선제적인 영웅대회 개최는 격변의 시기, 자칫 동요할 수 있는 인민들을 하나로 결속하게 만들었고, 나아가 북한의 체제 유지 및 강화에 긍정적으로 작용하였다. 이것이 탈영웅화하고 있는 현대사회에서 북한이 오늘날까지 영웅정치를 고수하고 있는 이유인 것으로 보인다. This study focuses on the three hero conventions held in North Korea's history to answer the question: why does North Korea still adhere to heroic politics in a modern society with a tendency of de-heroization? It examines the internal and external circumstances under which the North Korean authorities organised the hero conventions, the meaning of the hero conventions in the context of North Korean history, the exemplary heroes presented at each convention, and the tasks that the heroes ordered the people to perform. Evaluate whether each convention achieved the outcomes the North Korean authorities hoped for and consider how it has affected North Korea today. When North Korea was faced with major internal and external environmental changes that affected the survival of the regime, such as war, the fall of the socialist camp, economic crisis, and the march of hardship, the country held hero conventions to present exemplary heroes and set tasks for the people to perform with the heroes at the forefront. The 1953 National War Heroes' Convention, held just three weeks after the signing of the armistice, honoured the war heroes for their efforts in winning the war and set new goals for post-war recovery and national construction. In 1988, when the shadow of collapse was slowly falling on the socialist camp amidst an economic crisis, the economic heroes who already went through hardships with spirit were honoured and called upon to continue the 200-day battle into a 2,000-day and 20,000-day battle. After the hardship march, which remained an ‘unprecedented’ trial in world history, the Sun Gun Era Heroes' Convention, held in the new century in 2003, showcased the ideological heroes who did not betray the republic and the prime minister despite hardships and trials, and presented them as an example for the people to follow. By holding hero conventions preemptively, the people were able to get united. Otherwise, they could have been agitated during times of upheaval. Furthermore, it positively contributed to maintaining and strengthening of the North Korean regime. This seems to be the reason why North Korea has maintained its heroic politics to this day in a de-heroicising modern world.

고속철도소음의 예측과 평가에 관한 연구

배은혜 영남대학교 산업대학원 2006 국내석사

리듬교육이 아동의 창의성 발달에 미치는 영향 : 7세 아동 중심으로

배은혜 경북대학교 교육대학원 2010 국내석사

인터넷과 디지털 기술의 발달로 다양한 가치와 문화, 생활양식이 공존하는 정보화 시대에 필요한 인간상은 창의적인 사람이다. 그래서 창의성을 높이기 위한 교육의 필요성이 더욱 강조되고 있다. 아동의 다양한 교육활동 중 음악교육은 다른 활동에 비해 아동 창의성 발달에 크게 영향을 준다. 본 연구는 아동의 다양한 교육활동 중 가장 기본이며 중요한 리듬교육을 7세 아동에게 일정기간 수업하여 7세 아동 창의성 발달에 어떠한 영향을 주는지 실험 하였다. 대구광역시 북구 침산동에 위치한 A 유치원 7세 아동 a반 15명, b반 15명에게 10주 동안 1시간씩 10번 리듬교육을 수업하여 리듬교육 전, 후 창의성검사(TTCT)를 하였다. SPSS-win 14.0을 사용하여 창의성 점수를 분석한 결과 창의성 요소 중 유창성은 리듬교육 전과 비슷한 것으로 나왔으며 나머지 독창성, 추상성, 정교성, 개방성은 리듬교육 전 보다 창의성이 발달 된 것으로 나왔다. A 유치원 대조군으로 침산동에 위치한 B 어린이집을 대상으로 같은 기간 리듬교육을 하지 않고 창의성 검사를 실행하였다. 그 결과 리듬교육을 받은 A 유치원이 리듬교육을 받지 않은 B 어린이집보다 창의성이 더 높은 것으로 나왔다. 이 연구로 보아 리듬교육은 7세 아동 창의성에 영향을 끼치는 것으로 알 수 있으며 특히 창의성 중 독창성, 추상성, 정교성, 개방성 부분에 영향을 주는 것으로 결론이 난다. 이 논문으로 인해 현장에서 교육을 하시는 선생님과 유아음악에 관심을 가지는 학생들에게 도움이 되었으면 좋겠다. In the days of information and technology with various value system, culture and ways of life according to the development of internet and digital technology, creative person is desperately needed. So it is emphasized that there should be a growing need for education to develop creativity. Among various educational activities for children, musical education has a great influence on creativity development, compared with other educational activities. Through the experiment with 7 year-old children on rhythm education during a designated time, this study is to analyse how the rhythm education has an effect on the creativity development of children. The experiment was done with the 7 year-old children (a class : 15, b class : 15) of A kindergarten located in Chimsan-Dong, Buk-Gu, Daegu. Creativity Test (TTCT) was carried out before and after 10 classes (1hour/class) of rhythm education during 10 weeks. As a result of creativity test using SPSS-win 14.0, fluency in thinking of educated children showed similar result before they were educated, however originality, abstractness of titles, elaboration and resistance to premature closure of the children turned out to be developed after they were educated. The same creativity test was done with children of B kindergarten (control group) located in Chimsan-Dong who did not receive rhythm education. The result revealed that the children of 'A' kindergarten showed higher creativity than those of 'B' kindergarten. In conclusion, rhythm education affected creativity development of 7-year-old children, especially on originality, abstractness of titles, elaboration and resistance to premature closure. I hope that this study will be a help for teachers and students who are interested in musical education for children.

다중벽 탄소나노튜브(MWCNT) 도전재가 첨가된 실리콘-흑연 복합 음극 개발

배은혜 경북대학교 대학원 2025 국내석사

The electrochemical properties of Si-Gr composite anodes were investigated with the addition of multi-walled carbon nanotube (MWCNT) materials. To determine whether the addition of CNT alleviates the mechanical degradation caused by the volume expansion of silicon, the performance differences between non-CNT-added and CNT-added electrodes were examined using different ratios of active materials, GrSi6 and GrSi30. The GrSi6 with CNT electrode demonstrated higher capacity retention compared to the GrSi6 non-CNT-added electrode, even over long cycling periods. Similarly, the GrSi30 with CNT electrode also retained its capacity better than its non-CNT-added counterpart. To evaluate fast charge and discharge performance, the GrSi6 with CNT electrode was subjected to repeated charging and discharging at high current densities, followed by a return to low current densities. The electrode effectively recovered to its initial capacity, indicating that CNT reduces structural damage, thereby preserving capacity under repetitive charge-discharge conditions. Even at a high current density of 6C, the CNT-added electrodes showed higher overall average capacity compared to the non-CNT-added electrodes. Electrochemical impedance spectroscopy (EIS) results revealed that the addition of CNT enhances electrochemical conductivity by reducing charge transfer resistance and increasing ionic diffusivity. This suggests that the low electrical conductivity of silicon can be compensated by CNT addition, and mechanical degradation caused by volume expansion can be mitigated. Moreover, the capacity was well-maintained even at high current densities, which contributes to the overall capacity improvement. Further research on CNT materials as a solution to address capacity degradation due to silicon volume expansion is expected to facilitate the development of high-capacity, fast-charging batteries.

낮 병동 폭식증 환자를 위한 집단미술치료 : 대상관계 심리학 이론 중심으로

배은혜 명지대학교 사회교육대학원 2004 국내석사

본 연구는 낮 병동에 있는 폭식증 환자를 대상으로 미술치료를 진행한 사례논문이다. 본 연구자는 서울에 있는 섭식장애 클리닉 낮 병동에서 폭식증 환자를 위한 집단 미술치료를 진행하였으며, 폭식증의 근본적인 문제가 내담자에 초기대상관계에 기인한다는 생각으로 대상관계 심리학 이론과 연결 지어 미술치료를 진행하였다. 본 연구의 구체적인 목적을 살펴보면 다음과 같다. 첫째, 대상관계에 입각한 미술치료는 폭식증 환자의 참자기와 거짓자기에 어떤 영향을 미치는가? 둘째, 대상관계에 입각한 미술치료는 폭식증 환자의 개인적 심리적 발달에 어떤 영향을 미치는가? (H-T-P중심으로) 연구의 목적을 위해 위니캇에 대상관계 이론에 근거한 ‘참자기, 거짓자기’ 척도와 H-T-P 그림검사기법을 사용하였으며, 미술치료 회기를 통한 그림의 변화를 통해 이를 구체화시켰다. 이와 같은 방법에 따른 연구의 결과는 2가지로 요약된다. 첫째 대상관계에 입각한 미술치료는 폭식증 환자들에게 자기를 탐색할 수 있는 안전한 놀이적 공간을 제공함으로써 폭식증 환자들의 참자기의 발현을 도왔다. 둘째 대상관계에 입각한 미술치료에서의 초기대상관계 발달적 접근에 따른 비전형적인 매체의 사용은 각기 다른 맥락에서 초기발달에 문제가 있는 폭식증 환자들에게 각각 적절하게 사용됨으로써 개인적 심리적 발달을 도왔다. 이와 같은 연구의 결과는 폭식증 환자들의 초기대상관계의 이해와 미술치료 접근방식에 대한 정보는 제공하여준다. 또한 심리적 변화에 따른 폭식증 환자의 그림발달은 앞으로의 폭식증 환자를 위한 미술치료 연구에 도움이 될 수 있을 것이다. The aim of this article is to present an example of the Art Therapy for Bulimia Nervosa patients in day-care unit which based on Object Relations Theory. The author believed that a fundamental cause of Bulimia Nervosa is closely related to the patients' early object relations. Specific objectives are as follows : First, how will Object Relations Theory oriented Art Therapy influence the 'True self' and the 'False self' of patients with Bulimia Nervosa ? Second, how will Object Relations Theory oriented Art Therapy influence the individual and psychological development of the patients? (focusing on the results of H-T-P method) Winnicott's concept of 'True self and False self' was measured by usingH-T-P Picture Inspection Techniques. The results of the methods were embodied by changes shown in the patients' pictures in the therapy. The result of this study as follows: First, Object Relations Theory oriented Art Therapy provides an outlet for the patients to explore themselves, and help them manifest their true selves. Second, non-directive method with pre-art media in the Object Relations Theory oriented Art Therapy helps individual and psychological development when it is individually adapted for the patients having problems in their early development. Those results show how the Art Therapy for Bulimia Nervosa works in relation to the patients' early object relations. In particular, a different pattern observed in the patient's drawings during the Art Therapy gives an implication for future research into 'The Art Therapy for Bulimia Nervosa'.

Molecular rotor dynamics of electronically excited dyes in viscoelastic media

배은혜 이화여자대학교 대학원 2010 국내석사

1장에서는, 점탄성에 대한 분자 회전 역학의 의존성을 이해하기 위해 용액과 다양한 폴리머 상에서 여기상태의 trans-4-[4-(dimethylamino)-styryl]-1-methylpyridinium iodide (4-DASPI)의 회전을 연구하였다. 용액상에서는 점도를 변화시키기 위해 물과 글리세롤의 비율을 달리하였고 폴리머상에서는 10가지의 다른 폴리머 (low density polyethylene (LDPE), polyvinyl alcohol (PVA), high density polyethylene (HDPE), ultrahigh molecular weight polyethylene (UHMWPE), polyvinyl chloride (PVC), polycarbonate (PC), polycaprolactam (Nylon 6), poly(methyl methacrylate) (PMMA), polystyrene (PS), polyacrylic acid (PAA))를 사용하여 탄성을 변화시켰다. 여기된 4-DASPI의 회전 운동은 점도와 탄성도에 강한 상관관계를 보였다. 또한 우리의 결과는 4-DASPI의 압축된 상태의 역학은 점탄성 상에서 회전 운동과 물리적인 성질의 결합된 상태에 의해 지배되는 것을 보였다. 2장에서는, 점탄성 매질에서의 분자회전 역학과 작용기의 효과를 연구하기 위해 메틸기, 부틸기, 아다만탄 에틸기를 가진 새로운 9-(2-carboxy-2-cyanovinyl)-julolidine (CCVJ) 유도체를 합성하였다. 우리는 용액과 유기겔과 폴리머의 세가지 다른 매질을 사용하였다. 용액 상에서 에틸렌 글리콜과 글리세롤을 사용하였는데 점도를 변화시키기 위해 이 둘의 비율을 다양하게 하여 섞었다. 모든 분자들이 용액의 점도와 형광세기 사이에서 거듭제곱의 관계를 보였다. 양자수율은 점도가 20에서 990 cP로 증가함에 따라 10배가 증가하였고, 형광소멸시간은 점도가 215에서 990 cP로 증가함에 따라 2.5배가 증가하였다. 유기겔 상에서는, 겔형성기로 12-hydroxy stearic acid (HAS)를 사용하였고 용매로 톨루엔을 사용하였다. 점탄성을 조절하기 위해 HSA 겔형성기의 비율을 1에서 10 wt. % 로 바꾸어 주었다. 낮은 점탄성도에서 (Methyl-(2-carboxy-2-cyanovinyl)-julolidine (MCVJ) 의 양자수율은 (adamantyl-(2-carboxy-2-cyanovinyl)-julolidine (ACVJ) 의 양자수율보다 낮았지만 점탄성도가 350 kPa를 넘으면 이 둘의 양자수율은 같아진다. 폴리머상에서는 매질로 Decafluorobiphenyl-4,4-(hexafluoro-isopropylidene)-diphenol (DF-F) 가 사용되었다. 폴리머의 강도를 높이기 위해서 나노다이아몬드를 첨가하였다. 폴리머 나노복합물에 나노다이아몬드를 0.5에서 1.5 wt. % 까지 분산시켰다. MCVJ와 비교해 볼 때, ACVJ의 형광소멸시간은 탄성의 증가에 따라 현저히 증가하였다. 이 결과는 형광체에 더 큰 작용기가 있을수록 탄성도에 더 많은 영향을 받는다는 것을 의미한다. In chapter I, the twisting motion of trans-4-[4-(dimethylamino)-styryl]-1-methylpyridinium iodide (4-DASPI) in the excited state was investigated in solutions and various polymers in order to understand dependence of molecular rotor dynamics on viscoelasticity. We used different mixtures of water and glycerol to control viscosity as solvents media and ten different polymers, low density polyethylene (LDPE), polyvinyl alcohol (PVA), high density polyethylene (HDPE), ultrahigh molecular weight polyethylene (UHMWPE), polyvinyl chloride (PVC), polycarbonate (PC), polycaprolactam (Nylon 6), poly(methyl methacrylate) (PMMA), polystyrene (PS), and polyacrylic acid (PAA), were used to change Young’s modulus as polymer media. It was observed that the internal motion of electronically excited 4-DASPI correlate strongly with dynamic viscosity and elastic modulus. Our results also showed that condensed phase dynamics of 4-DASPI are governed by the explicit mode coupling between the rotamerizing coordinate and mechanical properties of viscoelastic media. In chapter II, new 9-(2-carboxy-2-cyanovinyl)-julolidine (CCVJ) derivatives bearing methyl, butyl and 1-adamantane ethyl group were synthesized to study molecular rotor dynamics and side-chain effects in viscoelastic media. We used three different media, solvents, organogels and polymers. As solvents, different mixtures of ethylene glycol and glycerol were used to modulate viscosity. All compounds show a power-law relationship of fluorescence emission with the viscosity of the solvent. The quantum yield increased about 10 times, as the viscosity increased from 20 to 990 cP, and lifetime increased about 2.5 times, as the viscosity increased from 215 to 990cP. In organogel media, we focused on 12-hydroxy stearic acid (HSA) and toluene as a gelator and a solvent, respectively. To control the elastic shear modulus, we change the HSA gelator proportion, ranging from 1 to 10 wt. %. The quantum yield of (Methyl-(2-carboxy-2-cyanovinyl)-julolidine (MCVJ) is smaller than that of (adamantyl-(2-carboxy-2-cyanovinyl)-julolidine (ACVJ) at low elastic shear modulus but it becomes almost same with ACVJ at above 350 kPa. Decafluorobiphenyl-4,4-(hexafluoroisopropylidene)-diphenol (DF-F) was used as polymer. We incorporated nanodiamond (ND) to polymer as a source of reinforcement for polymer composite materials. The polymer nanocomposites were dispersed with the ND ranging from 0.5 to 1.5 wt. % in content. In comparison with the MCVJ, the lifetime of ACVJ is increased more dramatically by Young’s modulus. It means that dye has bigger side chains, it is more effected by Young’s modulus.

Friend 마우스레트로바이러스에 의해 유도되는 합포체 형성 기작에 관한 연구

배은혜 단국대학교 2006 국내석사

마우스 레트로바이러스 중에 합포체(syncytia) 형성을 일으키는 것은 매우 드물지만, 레트로바이러스 중 병원성과 관련 있는 렌티바이러스 (예, HIV)에서는 일반적이다. 합포체를 유도하는 마우스 레트로바이러스는 병원성을 나타내지만, 합포체 형성을 야기 시키는 기작에 대해서는 잘 알려져 있지 않다. 합포체는 바이러스-세포간의 융합 또는 세포-세포간의 융합에 의해 형성되어지는 것으로 알려져 있다. 레트로바이러스에 의한 합포체의 형성을 연구하기 위해 합포체 형성을 유도하는 새로운 ecotropic 마우스레트로바이러스(Friend murine leukemia virus)의 변이주를 실험에 사용하였다. 마우스레트로바이러스의 외막에 존재하는 당단백질 중 수용체와 결합하는 부위의 아미노산을 변화시키면 합포체를 형성할 수 있음이 이미 밝혀졌다. 본 연구에서는 합포체유도 마우스레트로바이러스 외막 당단백질을 가진 pseudotype 레트로바이러스를 이용하여 합포체 형성여부를 알아보았다. 마우스 세포주인 M. dunni에 pseudotype바이러스를 감염시킨 결과 레트로바이러스 벡터를 매개로한 바이러스-세포간의 융합현상은 일어나지 않았다. 이러한 실험결과는 합포체 형성이 바이러스 복제가 가능한 합포체유도 마우스레트로바이러스에 의해서만 일어남을 나타낸다. 또한 ecotropic 마우스레트로바이러스 수용체의 농도와 막 융합과의 상관관계도 없는 것으로 밝혀졌다. Syncytia formation is relatively rare in mouse retroviruses but common in lentiviruses(e.g. HIV) where it is associated with pathogenicity. The syncytia inducing mouse viruses are also pathogenic, but the mechanism by which mouse retroviruses cause syncytia is not well known. Syncytium formation is believed to arise via virus-cell fusion or by cell-cell fusion. To study the mechanism of syncytium formation, novel syncytia-inducing ecotropic murine retrovirus was used. The previous result showed that amino acid substitutions at the RBD(receptor binding domain) of envelope glycoprotein contribute to syncytium formation. In this study, I have investigated if this fusion phenomenon could occur with retroviral vectors pseudotyped with the novel syncytia-inducing ecotropic murine leukemia virus Env. I have found that these vectors were not able to mediate virus-to-cell fusion in M. dunni murine cell lines. These findings indicate that syncytia-inducing ecotropic murine leukemia virus is capable of generating syncytia during its replication. There was also no correlation between the level of ecotropic murine leukemia virus receptor (dCAT-1) and the fusogenic effect.