RISS 학술연구정보서비스

검색
다국어 입력

http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.

변환된 중국어를 복사하여 사용하시면 됩니다.

예시)
  • 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
  • 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
닫기
    인기검색어 순위 펼치기

    RISS 인기검색어

      검색결과 좁혀 보기

      선택해제
      • 좁혀본 항목 보기순서

        • 원문유무
        • 원문제공처
          펼치기
        • 등재정보
        • 학술지명
          펼치기
        • 주제분류
        • 발행연도
          펼치기
        • 작성언어
        • 저자
          펼치기

      오늘 본 자료

      • 오늘 본 자료가 없습니다.
      더보기
      • 무료
      • 기관 내 무료
      • 유료
      • KCI등재

        동양달팽이 (Nesiohelix samarangae) 의 CO-I 유전자를 이용한 분자계통학적 연구

        방인석,이용석 한국패류학회 2014 The Korean Journal of Malacology Vol.30 No.4

        동양달팽이의 EST 서열 4개의 클론을 어셈블리하여 추출되어진 NsCO-I (partial)서열의 코딩 영역은 852 bp, 284개의아미노산으로 구성되어 있었다. BLAST 결과를 토대로 하여유사도가 높은 68개의 서열을 추출 하였으며 MEGA6 프로그램을 통해 clustalW 엔진을 통해 다중서열정열을 수행하고molecular phylogenetic analysis를 수행한 결과Heterobranchia, Nudibranchia, Cephalaspidea, Sacoglossa, Pulmonata 등의 카테고리별로 잘 분류 되었으며 Mastigeulota kiangsinensis, Helix aspersa, Cepaea nemoralis, Elona quimperiana, Camaena cicatricosa, Cylindrus obtusus 등 육산패류들과 잘 묶인다는 사실을 확인 할 수 있었다. Previously, we have reported expressed sequence tags (ESTs) analysis on the land snail, Nesiohelix samarangae (Ns). Of these ESTs, we have identified four partial fragments of N. samarangae cytochrome oxydase I (NsCO-I) gene which lead to obtain an 852 bp partial cDNA. Since NsCO-I is one of the best-known molecular phylogenetic markers, we have attempted to conduct comparative in silico analysis by using the NsCO-I gene. The combined results from BLAST analyses, multiple sequence alignment and molecular phylogenetic study of NsCO-I cDNA indicate that N. samarangae has similarity to three land snails such as Elona quimperiana, Euhadra herklotsi and Euhadra idzumonis.

      • KCI등재

        연체동물 유전체 연구현황

        방인석,한연수,이준상,이용석 한국패류학회 2010 The Korean Journal of Malacology Vol.28 No.3

        The availability of fast and inexpensive sequencing technology has enabled researchers around the world to conduct many genome sequencing and expressed sequence tag (EST) projects of diverse organisms. In recent years, whole genome projects have been undertaken to sequence ten species from the phylum Mollusca. These include Aplysia californica, Lottia gigantea, Crassostrea virginica, Spisula solidissima, Mytilus californianus, Biomphalaria glabrata, Crepidula fornicata, Elysia chlorotica, Lottia scutum and Radix balthica. Additionally, complete mitochondrial genomes of 91 mollusks have been reported. In Korea, EST projects have been conducted in nine mollusk species that include Nesiohelix samarangae, Pisidium (Neopisidium) coreanum, Physa acuta, Incilaria fruhstorferi, Meretrix lusoria, Ruditapes philippinarum, Nordotis gigantea, Crassostrea gigas and Laternula elliptica. Finally, the mitochondrial genome projects from the Pacific Oyster (Crassostrea gigas) and the rock shell (Thais clavigera) have been conducted and reported. However, no systemic mollusk genome project has so far been conducted in Korea. In this report, the current status and research trends in mollusk genome study in Korea will be discussed.

      • 바람의 산책 - 김후란과 문충성의 시

        방인석 본질과 현상사 2017 본질과 현상 Vol.50 No.-

        '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 9시까지 원문보기가 가능합니다.

      • KCI등재

        Expression and purification of Artogeia rapae lysozyme II cDNA in a baculovirus/insect cell system

        방인석,여성문,강창수 한국곤충학회 2006 Entomological Research Vol.36 No.2

        Previously, the Artogeia rapae lysozyme II (ARLII) gene was isolated and its complete nucleotide sequence was determined by RACE-PCR from fat body of larvae injected with Escherichia coli. In the present study, the ARLII gene was expressed by using a baculovirus expression vector system (BEVS). The expression level of recombinant (r)ARLII protein was optimized by varying virus titer and time-course of infection. The optimum protein expression conditions were infection of the cells at a multiplicity of infection of 10, and harvest at 84 h post-infection. Under these conditions, we estimated the amount of rARLII produced in the BEVS to be 10 mg/mL. rARLII was purified from cell-conditioned media using cation exchange column and reversed-phase FPLC methods. Purified rARLII was able to form a clear zone in a lysoplate assay against Micrococcus luteus. The lytic activity was estimated to be 1.53 times higher than that of hen egg white lysozyme (HEWLZ) under the same conditions. The optimum temperature for the lytic activity of the rARLII was 50°C, and its temperature dependency was greater than that of HEWLZ at low temperatures (<65°C).

      • KCI등재

        JAK/STAT signaling in insect innate immunity

        방인석 한국곤충학회 2019 Entomological Research Vol.49 No.8

        Lack of an adaptive strategy to combat infection creates opportunities for the innate immune system to guide invertebrate defense mechanisms. The innate immunity signaling cascades in invertebrates are elaborate, complex, and pathogen-specific. Among invertebrates, the most extended repertoire of molecules that function in the regulatory signaling pathways has been observed in insects. This is highlighted by the fact that antimicrobial peptide (AMP) production against pathogens is orchestrated through diverse immune pathways, either independently or through cross-talk mechanisms. The Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway coordinates immune responses from cytokines and regulates multiple homeostasis mechanisms in the host. That pathway has been implicated in the regulation of cell growth, differentiation, apoptosis, and inflammatory reactions. Many novel therapeutic interventions for tumors have been aimed at inhibitors of the JAK/STAT cascade. The regulatory pathway has much fewer components in Drosophila, and human homologs of almost all the critical pathway components and negative regulators have been identified. Loss-offunction mutation analysis and RNA interference-based gene silencing modeling have produced functional characterization of the core components and negative regulators in Drosophila melanogaster, Aedes aegypti, and Anopheles gambiae, and in some hymenopteran and lepidopteran species. The genome-wide analysis of the coleopteran species, Tribolium castaneum and Tenebrio molitor have been explored for elucidation of their JAK/STAT pathway regulatory components. Considering the promise of the JAK/STAT pathway in the mammalian model, the regulatory pathway in insects seems interesting especially for understanding pathogen surveillance mechanisms.

      • KCI등재

        Molecular Cloning and Characterization of Lysozyme II from Artogeia rapae and its Expression in Baculovirusinfected Insect Cells

        방인석,강창수 한국통합생물학회 2007 Animal cells and systems Vol.11 No.2

        Artogeiarapae was cloned from fat body of the larvae injected with E.coli and its nucleotide sequence was determined by theRACE-PCR. It has an open reading frame of 414 bpnucleotides corresponding to 138 amino acids including asignal sequence of 18 amino acids. The estimated molecularweight and the isoelectric point of the lysozyme I withoutthe signal peptide were 13,649.38 Da and 9.11, respectively.The A. rapae lysozyme II (ARL II) showed the highestidentity (81%) in the amino acid sequence to Manduca sextalysozyme among other lepidopteran species. The two catalyticresidues (Glu32 and Asp50) and the eight Cys residue motifs,which are highly conserved among other c-type lysozymesin invertebrates and vertebrates, are also completelyconserved. A phylogenetic analysis based on amino acidsequences indicated that the ARL I was more closelyrelated to M. sexta, Hyphantria cunea, Heliothis virescens,and Trichoplusia ni lysozymes. The ARL II gene wasexpressed in Spodoptera frugiperda 21 insect cells and therecombinant ARL I (rARL II) was purified from cell-conditionedmedia by cation exchange column chromatography andreverse phase FPLC. The purified rARL II was able to form aclear zone in lysoplate assay against Micrococcus luteus.The lytic activity was estimated to be 511.41 U/mg, 1.53times higher than that of the chicken lysozyme. Theoptimum temperature for the lytic activity of the rARL II was50oC, the temperature dependency of the absolute lyticactivity of rARL II was higher than that of the chickenlysozyme at low temperatures under 65oC.

      • KCI등재

        연체동물 유전체 연구현황

        방인석,한연수,이준상,이용석,Bang, In-Seok,Han, Yeon-Soo,Lee, Jun-Sang,Lee, Yong-Seok 한국패류학회 2010 The Korean Journal of Malacology Vol.26 No.4

        The availability of fast and inexpensive sequencing technology has enabled researchers around the world to conduct many genome sequencing and expressed sequence tag (EST) projects of diverse organisms. In recent years, whole genome projects have been undertaken to sequence ten species from the phylum Mollusca. These include Aplysia californica, Lottia gigantea, Crassostrea virginica, Spisula solidissima, Mytilus californianus, Biomphalaria glabrata, Crepidula fornicata, Elysia chlorotica, Lottia scutum and Radix balthica. Additionally, complete mitochondrial genomes of 91 mollusks have been reported. In Korea, EST projects have been conducted in nine mollusk species that include Nesiohelix samarangae, Pisidium (Neopisidium) coreanum, Physa acuta, Incilaria fruhstorferi, Meretrix lusoria, Ruditapes philippinarum, Nordotis gigantea, Crassostrea gigas and Laternula elliptica. Finally, the mitochondrial genome projects from the Pacific Oyster (Crassostrea gigas) and the rock shell (Thais clavigera) have been conducted and reported. However, no systemic mollusk genome project has so far been conducted in Korea. In this report, the current status and research trends in mollusk genome study in Korea will be discussed.

      • KCI등재

        Production of Recombinant Hinnavin II/MSH Hybrid in Insect Cells

        방인석,박승환,강창수,방선권 한국곤충학회 2005 Entomological Research Vol.35 No.1

        The increasing problem of antibiotic resistance among pathogenic bacteria requires development of new antimicrobial agents. For the purpose of this study, a cDNA encoding hinnavin II-α-melanocyte stimulating hormone (hin/MSH) hybrid was chemically synthesized, annealed, and then cloned into transfer vector pBacPAK 9 for expression in Sf21 insect cells. Recombinant hin/MSH (rhin/MSH) hybrid was efficiently produced in baculovirus expression vector system (BEVS) as a hybrid peptide. The antibacterial activity of the rhin/MSH hybrid was compared to that of the recombinant hinnavin II (rhin), using inhibition zone and overlay assay. This new recombinant hybrid peptide may serve as an attractive candidate for powerful novel class of antimicrobial pharmaceuticals.

      연관 검색어 추천

      이 검색어로 많이 본 자료

      활용도 높은 자료

      해외이동버튼