연체동물 유전체 연구현황

방인석,한연수,이준상,이용석 한국패류학회 2010 The Korean Journal of Malacology Vol.28 No.3

The availability of fast and inexpensive sequencing technology has enabled researchers around the world to conduct many genome sequencing and expressed sequence tag (EST) projects of diverse organisms. In recent years, whole genome projects have been undertaken to sequence ten species from the phylum Mollusca. These include Aplysia californica, Lottia gigantea, Crassostrea virginica, Spisula solidissima, Mytilus californianus, Biomphalaria glabrata, Crepidula fornicata, Elysia chlorotica, Lottia scutum and Radix balthica. Additionally, complete mitochondrial genomes of 91 mollusks have been reported. In Korea, EST projects have been conducted in nine mollusk species that include Nesiohelix samarangae, Pisidium (Neopisidium) coreanum, Physa acuta, Incilaria fruhstorferi, Meretrix lusoria, Ruditapes philippinarum, Nordotis gigantea, Crassostrea gigas and Laternula elliptica. Finally, the mitochondrial genome projects from the Pacific Oyster (Crassostrea gigas) and the rock shell (Thais clavigera) have been conducted and reported. However, no systemic mollusk genome project has so far been conducted in Korea. In this report, the current status and research trends in mollusk genome study in Korea will be discussed.

'탈식민주의의 3대 이론서에 대한 동양적 해석'에 대한 토론문

방인석 국제어문학회 ( 구 국제어문학연구회 ) 2011 국제어문학회 학술대회 자료집 Vol.2011 No.2

삼차신경초종 1 예

방인석,이정청 전북대학교 의과학연구소 1983 全北醫大論文集 Vol.7 No.1

Trigeminal neurinoma located only in the cerebello-pontine angle is rarely encountered and it usually accompanies cerebellar signs. Trigeminal neurinoma is commonly arised from the Gasserian ganglion and located in the middle cranial fossa or extended into the posterior cranial fossa as dumbbell shape. A rare case of trigeminal neurinoma located only in the right cerebello-pontine angle without any evidences of cerebellar signs is presented. The patient was 65 year-old man who had trigeminal neuralgia on the right mandibular area. The tumor arised from the sheath of the right trigeminal nerve root of the cerebellopontine angle. It was successfully removed without injury to the nerve itself under the surgical microscope.

중대뇌동맥폐색에 시행한 두개강내외 동맥문합술의 경험

방인석,이정청 전북대학교 의과학연구소 1982 全北醫大論文集 Vol.6 No.2

The authors have experienced EIAB surgery on two cases with middle cerebral artery occlusion. A 47 year-old male had neurological deficits such as slurred speech and the right hemiparesis and another 35 year-old male had the left hemiparesis. Both cases were completely occluded at genu portion of the middle cerebral artery, confirmed by cerebral angiography. Neurological improvement was followed within a few days after operation in two cases and moderate increment of collateral cerebral blood flow was demonstrated by postoperative cerebral angiography.

김수영 문학에 나타난 ‘사랑’과 ‘죽음’

방인석 우리문학회 2019 우리文學硏究 Vol.0 No.61

The key concepts that can identify Kim Soo-young's literature are sadness, sorrow, freedom, love, and death. In Kim Soo-young's literature, sadness, sorrow, freedom, love, and death are not merely individual values, but maintain a complementary relationship with other values and organically combine to form an integrated value system. In this paper, I take a closer look at the meaning of ‘love’ and ‘death’ in Kim Soo-young's literature by actively reviewing the organic correlations of the core concepts of Kim Soo-young's approach. Kim Soo-young's approach to literature is a process of discovering, exercising, and realizing love. Kim Soo-young emphasizes that ‘love’ is realized at the same time as the ‘death’ of the world, The newness of poetry is born at the point where death and love face each other. Love is not a step-by-step supplement after death, but an object that must be realized at the same time as death. Therefore, death is the boundary of poetry's newness and oldness, and love is the purpose that a new poem must pursue. Kim Soo-young's emphasis on death by focusing on poetry's novelty is because death is the only way to escape from a palliative world . He finds the poem's poem in love and death, and asks the poet to “finish it in his own style”, that is, his own death. There is no form unique to death. The poet does not go toward ultimate death, but toward creating his own death in his own way. For Kim Soo-young, the poet is a demonstrator of death, and poetry is the form of death he creates. In this paper, Kim Soo-young's poetry continuously extends the individuality of ‘I’ to pursue the existence of the individual while experiencing the transparency of ‘I’ and the anonymity of the other person, thereby realizing the finitude of the subject and finally overcoming the alienation from oneself through the meaning of ‘love’ and ‘death’. 김수영의 문학을 규명할 수 있는 핵심 개념은 설움, 비애, 자유, 사랑, 죽음 등 다양하다. 김수영의 문학에서 설움, 비애, 자유, 사랑, 죽음 등은 개별적으로 충일한 가치가 아니라 다른 가치들과 상보적 관계를 유지하며 유기적으로 결합하면서 통합적 가치 체계를 형성한다. 본 연구는 주제론적 접근을 통해 이룩한 그간의 연구 성과를 충실히 받아들이는 한편, 김수영의 문학을 규명하는 핵심 개념들의 유기적 상관성을 적극적으로 검토함으로써 김수영의 문학에 나타난 ‘사랑’과 ‘죽음’의 의미에 접근한다. 이는 기본적으로 김수영의 문학이 사랑을 발견하고 행사하고 실현하는 과정임을 전제로 한다. 김수영은 시의 새로움을 한 세계의 ‘죽음’과 동시에 실현되는 ‘사랑’임을 강조한다. 시의 새로움은 죽음과 사랑이 서로 마주 대하는 지점에서 탄생한다. 사랑은 죽음 이후에 오는 단계적 보충물이 아니라 죽음과 동시에 실현되어야 할 구체적 대상이다. 그러므로 죽음은 시의 새로움과 낡음의 경계이며 사랑은 새로운 시가 추구해야 할 목적이다. 김수영이 시의 새로움을 논하며 죽음을 강조하는 이유는, 죽음만이 고식적(姑息的)인 세계와 고답적(高踏的)인 관념으로부터 벗어날 수 있는 유일한 통로이기 때문이다. 그는 시의 ‘본수(本髓)’를 사랑과 죽음에서 찾았으며 시인에게 ‘죽음을 딛고 일어선 자기만의 스타일’, 즉 자기만의 죽음을 완성할 것을 요구한다. 죽음에 고유한 형식이란 존재하지 않는다. 시인은 이미 마련된 궁극적 죽음을 향해 나아가는 것이 아니라 자기 방식대로, 자기만의 죽음을 창조하는 것이다. 김수영에게 시인은 죽음을 시연(試演)하는 자이며 시는 그가 창조한 죽음의 형식이다. 본고는 김수영의 시가 ‘나’의 개별성을 끊임없이 연장시켜 개인의 실존을 추구하는 한편 ‘나’의 투명성과 타자의 익명성을 경험함으로써 주체의 유한성을 자각하고 마침내 자기 자신으로부터의 소외를 극복하는 과정을 ‘사랑’과 ‘죽음’의 의미를 통해 규명한다.

배추흰나비 (Pieris rapae L.)의 acid phosphatase 활성 및 정제

방인석,여성문 한국곤충학회 1990 Entomological Research Vol.20 No.4

배추흰나비(Pieris rapae L.)의 유충 및 용기의 발생 단계에 따른 acid phosphatase의 활성은 4령 말기가 5령 초기보다 높게 나타나고 그후 점차 증가하여 5령 말기에 최대의 활성을 보인 후 용화 후로는 감소하기 시작하였다. Acid phosphatase는 4개의 isozyme으로 구분되었고 그중 Acph$^2$ (acid phosphatase isozyme II)가 주된 효소로 존재하였다. 배추흰나비의 5령 말기 유충에서의 acid phosphatase분포는 중장, 지방체, 타액선 및 혈림프 등에서 확인되었으며, ion exchange chromatography와 gel filtration 및 gel slicing을 통해 정제된 Acph$^2$의 최종 순도는 약 25배에 이르렀다. The acid phosphatase activities in the late 4th instar larvae were higher than those in the early 5th instar larvae of Pieris rapae. They showed the highest in the late 5th instar larvae and were decreased during the pupal stage. Four isozymes of acid phosphatase were identified and Acph$^2$ (acid phosphatase isozyme II) revealed a major enzyme. The acid phosphatase of the late 5th instar larvae of Pieris rapae was found in midgut, fat body, salivary gland and haemolymph. The find purity of Acph$^2$ was estimated to be 25 fold by ion exchange chromatography, gel filtration and gel slicing.

Expression and purification of Artogeia rapae lysozyme II cDNA in a baculovirus/insect cell system

방인석,여성문,강창수 한국곤충학회 2006 Entomological Research Vol.36 No.2

Previously, the Artogeia rapae lysozyme II (ARLII) gene was isolated and its complete nucleotide sequence was determined by RACE-PCR from fat body of larvae injected with Escherichia coli. In the present study, the ARLII gene was expressed by using a baculovirus expression vector system (BEVS). The expression level of recombinant (r)ARLII protein was optimized by varying virus titer and time-course of infection. The optimum protein expression conditions were infection of the cells at a multiplicity of infection of 10, and harvest at 84 h post-infection. Under these conditions, we estimated the amount of rARLII produced in the BEVS to be 10 mg/mL. rARLII was purified from cell-conditioned media using cation exchange column and reversed-phase FPLC methods. Purified rARLII was able to form a clear zone in a lysoplate assay against Micrococcus luteus. The lytic activity was estimated to be 1.53 times higher than that of hen egg white lysozyme (HEWLZ) under the same conditions. The optimum temperature for the lytic activity of the rARLII was 50°C, and its temperature dependency was greater than that of HEWLZ at low temperatures (<65°C).

연체동물 유전체 연구현황

방인석,한연수,이준상,이용석,Bang, In-Seok,Han, Yeon-Soo,Lee, Jun-Sang,Lee, Yong-Seok 한국패류학회 2010 The Korean Journal of Malacology Vol.26 No.4

The availability of fast and inexpensive sequencing technology has enabled researchers around the world to conduct many genome sequencing and expressed sequence tag (EST) projects of diverse organisms. In recent years, whole genome projects have been undertaken to sequence ten species from the phylum Mollusca. These include Aplysia californica, Lottia gigantea, Crassostrea virginica, Spisula solidissima, Mytilus californianus, Biomphalaria glabrata, Crepidula fornicata, Elysia chlorotica, Lottia scutum and Radix balthica. Additionally, complete mitochondrial genomes of 91 mollusks have been reported. In Korea, EST projects have been conducted in nine mollusk species that include Nesiohelix samarangae, Pisidium (Neopisidium) coreanum, Physa acuta, Incilaria fruhstorferi, Meretrix lusoria, Ruditapes philippinarum, Nordotis gigantea, Crassostrea gigas and Laternula elliptica. Finally, the mitochondrial genome projects from the Pacific Oyster (Crassostrea gigas) and the rock shell (Thais clavigera) have been conducted and reported. However, no systemic mollusk genome project has so far been conducted in Korea. In this report, the current status and research trends in mollusk genome study in Korea will be discussed.

Purification and cDNA Cloning of Lysozyme II from Cabbage Butterfly, Artogeia rapae Larvae

방인석,여성문 한국곤충학회 2005 Entomological Research Vol.35 No.4

Last instar larvae of cabbage butterfly Artogeia rapae respond to injection of bacteria with a set of inducible antibacterial peptides/proteins. The inducible peptides/proteins are related to the known hinnavins (I and II) and lysozymes (I and II). The lysozyme II has been isolated by heat treatment, cation exchange, and reversed-phase chromatography from immunized hemolymph of last instar larvae. The lysozyme II gene of A. rapae was isolated and its nucleotide sequence was determined by the RACE-PCR from immunized fat body with E. coli. It has an open reading frame of 414 bp nucleotide corresponding to 138 amino acids including an 18 amino acid signal sequence. The molecular weight and the isoelectric point of Artogeia lysozyme II without asignal peptide were 13,649.38 Da and 9.11, respectively. It is great similarity with Manduca lysozyme among other lepidopteran.

Production of Recombinant Hinnavin II/MSH Hybrid in Insect Cells

방인석,박승환,강창수,방선권 한국곤충학회 2005 Entomological Research Vol.35 No.1

The increasing problem of antibiotic resistance among pathogenic bacteria requires development of new antimicrobial agents. For the purpose of this study, a cDNA encoding hinnavin II-α-melanocyte stimulating hormone (hin/MSH) hybrid was chemically synthesized, annealed, and then cloned into transfer vector pBacPAK 9 for expression in Sf21 insect cells. Recombinant hin/MSH (rhin/MSH) hybrid was efficiently produced in baculovirus expression vector system (BEVS) as a hybrid peptide. The antibacterial activity of the rhin/MSH hybrid was compared to that of the recombinant hinnavin II (rhin), using inhibition zone and overlay assay. This new recombinant hybrid peptide may serve as an attractive candidate for powerful novel class of antimicrobial pharmaceuticals.