신단백질(新蛋白質) 사료(飼料)의 개발(開發) 및 이용효율(利用效率)에 관(關)한 연구(硏究) -1. 재래산양(在來山羊)에 유용(有用)한 비단백태실소(非蛋白態室素)의 개발(開發)에 관(關)한 연구(硏究)-

방극승 ( Keuk Seung Bang ),김영길 ( Young Kil Kim ) 東亞大學校附設 石堂傳統文化硏究院 1978 石堂論叢 Vol.2 No.-

우수한 비단백대 질소를 개발 在來山羊에 有用하게 이용하고자 뇨소 피복뇨소 및 DUIB의 안전성과 적응성을 검토한 바 다음과 같은 結論을 얻었다. 1. 胃液의 pH는 NPN투여 1시간 후 최대치를 보였으며 뇨소 피복뇨소는 Alkali化하는 경향이었으나 DUIB는 별 영향이 없었다. 2. 胃液의 암모니아 농도는 體重 kg當 피복뇨소 0.77gm DUIB 1.28gm 까지 안전하였으며 뇨소 0.3gm도 약간의 위험성이 있었다. 3. 血液의 암모니아 농도는 體重 kg當 뇨소 .03gm 피복뇨소 0.77gm DUIB 1.28gm까지 안전하였다. 4. 在來山羊은 體重 kg當 뇨소 0.9gm 피복뇨소 2.33gm을 투여시 40분부터 80分 사이에 심한 중독증상을 나타냈다. 5. 뇨소 0.3gm(체중 kg當) 피복뇨소 0.77gm을 4주간 급여 한 바 胃液의 pH와 혈액의 암모니아 농도는 감소하였다. (P<0.05) 6. 以上의 結果로 볼 때 DUIB는 가장 우수한 非蛋白態 室素로 開發되어야 할 것이다. This experiment was conducted in effort to determine safety and proper amount of Urea, Coating urea and Diuredo-isobuane (DUIB) in Korean Native Goats. The results obtained were as follows: 1. Ruminal pH values were considerably higher (7.0-8.0) on urea and coating urea treated goats. But constant ruminal pH values were recorded on DUIB treated goats. 2. Ruminal ammonium nitrogen concentration were below toxic levels when treated with coating urea (0.77 gm/kg B. W.) and DUIB (1.28 gm/kg B. V.). Although urea (0.3 gm/kg B. W.) were slightly above toxic levels. 3. Blood ammonium nitrogen concentration were below toxic levels when treated with urea (0.3 gm/kg B. W.) coating urea (0.77 gm/kg B. W.)and DUIB (1.28 gm/B. W.) 4. Korean Native Goats used were intoxicated by 0.9 gm urea per kg B. W. and 2.33 gm coating urea per kg B. W. between 40 minutes and 80 minutes after administration. 5. Initial rise in ruminal pH and blood ammonium nitrogen concentration was a tendency for a decline after 4 weeks feeding urea (0.3 gm/kg B. W.) and coating urea (0.77 gm/kg B. W.) (p<0.05). The present data reveal that DUIB is most efficient NPN resource in korean Native Goats.

Influence of Transferred Anti-Nematospiroides dubius Immune Serum and IgG on Immune Reaction in Recipient Mice

Keuk Seung Bang(방극승) 대한기생충학열대의학회 1982 The Korean Journal of Parasitology Vol.20 No.2

유산양(乳山羊)에 있어 농후사료(濃厚飼料) 다급시(多給時) 중조(重曹)의 급여(給與)가 유생산량(乳生産量) 및 반추위생리(反芻胃生理)에 미치는 영향(影響)

최경문 ( Kyung Moon Choi ),김영길 ( Young Kil Kim ),방극승 ( Keuk Seung Bang ) 東亞大學校附設 石堂傳統文化硏究院 1977 石堂論叢 Vol.1 No.-

乳山羊에 있어서 濃厚飼料 多給時에 除酸劑인 重曹를 投與함으로써 胃內 protozoa 變化 胃液의 pH 總揮發性脂肪酸 生成量 乳量 및 乳質 등에 미치는 影響을 究明코자 粗飼料多給區(T1) 濃厚飼料 多給區(T2) 重曹處理區(T3)의 3處理에 乳山羊 3頭씩 配置하여 飼養實驗을 實施했던 바 그 結果를 要約하면 다음과 같다. 1. 試驗期間中 供試畜의 體重은 處理가 달라정도 큰 變化가 없었으며 處理別 1日 頭當 TND 攝取量은 T2區와 T3區보다 높았다. 2. 乳量, 乳比重 SNF의 含量은 處理別로 큰 差異가 없었으나 乳脂肪은 U2區가 3.1%로 有意的으로 (p<0.05) 낮고 重曹의 投與로 乳脂肪이 3.4%로 向上되었다. 3. 胃液의 pH는 T2區가 5.9로서 T1區에 比해 有意的(p<0.05) 낮으며 重曹를 投與함으로써 pH가 현저히 增加하였으며 總 VFA의 含量은 個體間에 差異가 컸으며 處理間에는 有意性이 없었다. 4. 重曹를 投與하였을 때 Rumen內의 總 protozoa는 현저히 增加하였으나 microfauna의 변동에는 아무런 影響을 미치지 못하였다. The experiment was conducted to study the effect of high concentrate feeding and of sodium bicarbonate supplement to milking goat on rumen protozoa change, rumen fluid pH, Total VFA production, milk production and milk composition with 3 heads of milking goats. The three treatments were composed of high roughage ration (T1) and high concentrate ration (T2) and high concentrate ration supplemented with sodium bicarbonate (T3). The results obtained are as follows: 1. The body weight of milking goats was not changed remarkably by the different treatment and the daily TDN intake per animal of T2 group was much greater than those of T1 and T3 group. 2. Milk production, specipic gravity, and solid-not-fat concentration was not affected significantly by different treatment. The milk fat concentration of high concentrate feeding group was 3.1% on an average which was significantly (p<0.05) low value than that of high roughage feeding group. The supplement of sodium bicarbonate increased the milk fat to 3.4%. 3. The pH of rumen fluid (5.9) of T2 group was significantly(p<0.05) lowered compared with T1 group and sodium bicarbonate increased pH value remarkably. 4. The sodium bicarbonate supplement increased the number of total protozoa and, however, was unable to affect the change of microfauna.

감자 Polyphenol Oxidase의 열안정성

김나영(Na-Young Kim),이민경(Min-Kyung Lee),박인식(Inshik Park),방극승(Keuk-Seung Bang),김석환(Seok-Hwan Kim) 한국식품영양과학회 2001 한국식품영양과학회지 Vol.30 No.5

감자로부터 polyphenol oxidase를 추출하여 열안정성에 영향을 주는 요인을 식품의 제조공정에 유용한 정보를 제공하기 위해 연구를 하였다. 감자의 PPO는 pH 7.0에서 가장 안정하였고, 이 효소는 80℃에서 1분간의 열처리로 효소 활성이 70% 저해되었다. 감자 PPO의 열안정성에 대한 z-value는 12.17±0.58℃이고, 이 효소의 열안정성은 sodium chloride의 첨가로 감소되었으며, 2-mercaptoethanol과 dithiothreitol과 같은 환원제의 첨가로 역시 효소활성이 저해되었다. Factors affecting thermostability of polyphenol oxidase (PPO) from potato were studied for the purpose of providing useful information for food processing operations. The enzyme was most stable at pH 7.0 and it was inhibited to 70% after heat treatment at 80℃ for 1 min. The z-value for the thermal inactivation of the PPO was 12.17±0.58℃. The thermostability of the enzyme was reduced by addition of sodium chloride. And the activity was inhibited by addition of reducing reagents such as 2-mercaptoethanol and dithiothreitol.

在來山羊의 飼育能力 向上에 관한 硏究

崔暻文,金榮吉,房極勝 東亞大學校 大學院 1977 大學院論文集 Vol.1 No.-

Experiment were conducted on Korean Native Goat to ascertain the effect of ovine coccidiosis on feedlot Kids. The results obtained were summarized as follows: 1. Oocyst production in each groups of kids reached level of 3 and 4 million oocyst per gram feces 18 to 22 days after kids entered in feedlot. 2. The parasitic development of coccidia was suppressed by SDM significantly (P 0.01) within 1 weeks after drug administration. 3. Hemoglobin and hematocrit value were altered in affected kids but changes in group average were not statistically significant. Total serum protein concentraion were decreased steadly throughout experiment period though SDM suppressed oocyst development within 1 week. 4. Adequate immunity did not develop in any of kids under 6 months old when challange inoculated with 500 thousand oocyst.

Polyethyleneglycol을 이용한 Immunoglobulin 정량법

房極勝,崔震浹,金正基 東亞大學校 大學院 1984 大學院論文集 Vol.8 No.2

A single and rapid method for quantative determination of immunoglobulin in serum is described. The method uses polyethyleneglycol which causes precipitation of immunoglobulin and investigation into electrophorectic pattern of serum by polyethyleneglycol and correlation between total protein concentration and immunoglobulin concentration have been performed. The polyethyleneglycol precipitation test described provides a rapid, accurate and precise way to measure immunoglobulin.

宿主의 性Hormone이 소진드기 Boophilus microplus의 산란에 미치는 영향에 대한 실험적 연구

房極勝 東亞大學校 1975 東亞論叢 Vol.12 No.2

性 Hormone齊를 소진드기 Boophilus microplus에 주사 하여 본 결과 다음과 같다. 1. Estrogen은 産卵前 휴식기간을 연장하고 산란을 억제 하는 效果가 있었다. 2. Progestron은 産卵活動에 뚜렷한 영향을 주지 않았다. 3. Testosterone 은 진드기의 初期 産卵을 촉진하는 效果가 있었다. 4. 性 Hormone이 産卵 지속기간, 産卵한 卵數에는 영향을 주지 않는 것으로 나타났다. This study was conducted to study the effect of host sexual hormone on egg laying of cattle tick. Boophilus microplus. The results were as follows: 1. Female sex hormone, Estradiol, markedly inhibited egg laying and resting period for egg laying. 2. Female sex hormone, Progestrone, showed no significant effect on egg laying activity. 3. Male sex hormone, Testosterone, acclerated egg laying especially in the early stage of egg laying period. 4. Above three sexual hormone showed no significant effect on the number of egg laid and duration of egg laying period.

新蛋白質 飼料의 開發 및 利用效率에 關한 硏究

房極勝,金榮吉 東亞大學校 石堂學術硏究奬勵會 1977 石堂論叢 Vol.2 No.-

This experiment was conducted in effort to determine safety and proper amount of Urea, Coating urea and Diuredo-isobuane (DUIB) in Korean Native Goats. The results obtained were as follows: 1. Ruminal pH values were considerably higher (7.0-8.0) on area and coating area treated goats. But constant ruminal pH values were recorded on DUIB treated goats. 2. Ruminal ammonium nitrogen concentration were below toxic levels when treated with coating urea (0.77 gm/kg B.W.) and DUIB (1.28 gm/kg B.W.). Although urea (0.3 gm/kg B.W.) were slightly above toxic levels. 3. Blood ammonium nitrogen concentration were below toxic levels when treated with urea (0.3 gm/kg B.W.) coating urea (0.77 gm/kg B.W.) and DUIB (1.28 gm/kg B.W.) 4. Korean Native Goats used were intoxicated by 0.9 gm urea per kg B.W. and 2.33 gm coating urea per kg B.W. between 40 minutes and 80 minutes after administration. 5. Initial rise in ruminal pH and blood ammonium nitrogen concentration was a tendency for a decline after 4 weeks feeding urea (0.3 gm/kg B.W.) and coating urea (0.77 gm/kg B.W.) (p<0.05). The present data reveal that DUIB is most efficient NPN resource in Korean Native Goats.

組大豆 Lecithin의 飼料化에 관한 硏究

金榮吉,房極勝,金大鎭 東亞大學校 大學院 1978 大學院論文集 Vol.2 No.-

The Research was conducted to study the feeding value of crude lecithin produced as by product of soybean oil manufacturing. The results obtined were as follows. 1. The chemical composition, mineral composition vitamin contents, gross energy and metabolizable energy values of crude lecithin were determined. 2. No significant difference was observed for the body weight gain of chicks between control and lecithin groups of 3%, 6%,9% and 12% addition. 3. Significant difference(p<0.05) was observed for feed intake between lecithin 12% and lecithin 6% and 9% group. However, No significant difference was found among control, lecithin 3%.6% and 9% groups. 4. No difference was found for feed requirement between treatments. However, the lowest figure was 2.32 for lecithin 9% treatment, followed by 2.40 of lecithin 6% and 2.41 of lecithin 3%, orderly. 5. No difference was found between treatments for RBC, WBC, hemoglobin and hematocrit value. 6. The composition of moisture, crude protein and ether extract of chicks ranged from 71.2 to 72.15%, from 15.93 to 16.45%, and from 6.19 to 7.05%, respectively. 7. The weight of abdominal fat and liver fat of chicks ranged from 42.4 to 63.7gm. and from 2.2 to 2.4gm, respectively, showing insignificant difference. 8. Total of 9 feed manufacturers used lecithin as energy source for poultry ration and each manufacturer employed somewhat different mixing method. 9. The patented bleaching reagents of crude lecithin were hydrogen peroxide and dibenzoyl peroxide. The bleaching facilities of crude lecithin with the capacity of 2M/T per day was calculated to amount to 3,650,000won.

Bifibacterium infantis 및 Streptococcus thermophilus 가 밀가루 반죽의 물성적 성질에 미치는 영향

김경자,홍정훈,방극승 한국조리과학회 2000 한국식품조리과학회지 Vol.16 No.1

1. 상온에서 시료 밀가루의 물결합능력은 250%이며 용해도는 1.0%였다. 2. Farinograph-본 실험에 사용된 밀가루 반죽의 v/v는 69였다. S. thermophilus가 첨가된 경우 대조군과 유사한 값인 70을 나타내었으며 B. infantis만 첨가된 군과 B. infantis + S. thermophilus 첨가군에서는 각각 65, 62로 나타났다. 반죽이 일정한 굳기에 도달하는데 필요한 수분 흡수율은 대조군이 66.5인데 비해 B. infantis + S. thermphilus군의 경우 67.8로 증가하였다. Peak time의 경우 모든 군이 6∼8분 안에 들어가면서 반죽의 탄력이 증가한 것으로 보인다. B. infantis 첨가군과 B. infantis + S. thermophilus첨가군은 각각 6.0과 5.5로 나타났으며 안정도의 경우 41.5와 40.1로 나타났다. 3. Extenspgraph-B. infantis 첨가군에서 90분과 135분 사이에 큰폭으로 상승되었다. 135분에서 S. thermophilus 첨가군이 가장 면적이 넓었으며 다음으로 B. infantis 첨가군과 B. infantis + S. thermophilus첨가군의 순서였다. 4. Amylograph-B. infantis 첨가군과 S. thermophilus 첨가군은 대조군에 비해 최고 점도가 높게 나타났으므로 빵의 내상이 균일하고 기공배열이 가지런하므로 전체적으로 품질이 좋은 빵이 될것으로 사료된다. 호화개시온도의 경우 대조군 비해 다소 낮아진 것으로 나타났다. 최고점 온도는 각 군 별로 차이가 없었다. In order to economically utilize dough with B. infantis and S. thermophilus as a bread improver, WHC, swelling power, solubility, farinograph, extensograph and amylograph of dough were investigated. Swelling power and solubility were less than medium flour and weak flour. WHC was higher than those. On rheological properties of dough, farinograms of dough showed progressively increasing water absorption, peak time only increased with addition of S. thermophilus. Extensograms showed that area increased S. thermophilus and B. infantis at 135 min. Extensibility and resistance to exntension of dough were higher in S. thermophilus than those in B. infantis. On amylograms, maximum viscosity of dough increased by addition of B. infantis and S. thermophilus