Vibrio parahaemolyticus의 전균체 및 편모항원에 대한 항혈청의 민감성과 특이성에 관한 연구

朴抱鉉,宋哲鏞,金鎬薰 中央大學校 自然科學硏究所 1988 自然科學硏究所 論文集 Vol.2 No.-

This study was undertaken to examine the sensitvity and specificity of antisera against whole cell antigen (WCA) and flagella antigen (FA) of Vibrio parahaemolyticus. Antiserum against WCA of V. parahaemolyticus showed 8-fold high agglutination titre with homologous H-antigen (1:25,600), while low cross-reactions were obesrved with H-antigen of W. alginolyicus (1:3,200), V. furnissii (1:200) and V. vulnificus (1:100). The antiWCA serum showed not only 2-fold agglutination titre with homologous O-antigen (1:320), but also showed high cross-reactions were observed with V. holisae (1:160), V. vulnificus (1:10), V. alginolyticus (1:10) and V. cholerae (1:10). The anti-WCA serum of V. aprahaemolyticus which absorbed with homologen O-antigen and heterologous H-antigen of V. alhinolyticus showed 32-fold high agglutination titre with homologous H-antigen (1:L 12,800). whereas heterologous reaction with /v. alhinolticus decreased from 1:3,200 to 1:200. Flagella antiserum against V. parahaemlyticus did not reacted with H-antigens of other Vibrio specices. Both absorbed anti-WCA serum and flagella antiserum were reacted specifically with 32 stains of V. parahaemoluticus isolated from patients other specimens. This result indicated that the antisera against whole cell antigen of V. parahaemolyticus followed by absorption with homologus O-antigen and H antigen V. alginolyticus and flagella antigen is preferable to O-antisera in respect of sensitivity and specificity.

경기도내에서 분리한 캠필로박터 제주니균의 유전적특성 및 항생제내성 연구

허은선,박포현,김종화,손종성,윤희정,이예은,최연숙,윤미혜,이정복,Hur, Eun-Seon,Park, Po-Hyun,Kim, Jong-Hwa,Son, Jong-Sung,Yun, Hee-Jeong,Lee, Yea-Eun,Choi, Yun-Sook,Yoon, Mi-Hye,Lee, Jong-Bok 한국미생물학회 2013 미생물학회지 Vol.49 No.3

Campylobacter jejuni는 사람에서 매우 중요한 식중독 원인균의 하나이며, 2010년 경기도내 병원을 내원한 설사 환자와 4번의 식중독 outbreak에서 42균주를 분리하였다. 본 연구에서는 분리된 C. jejuni 42균주의 유전적 특성과 혈청형, 항균제 내성율을 분석하였다. hipO 종특이 유전자(100%), cdtB 독소 유전자(100%)가 검출되었고, gyrA 돌연변이 유전자(95.2%)가 PCR 실험결과 검출되었다. gyrA 돌연변이 유전자는 ciprofloxacin 내성과 연관이 깊으며, 디스크 확산법에 의해 실험한 결과 gyrA 돌연변이 유전자가 검출된 40균주(95.2%)에서 실제 ciprofloxacin에 대해 내성을 보였다. 분리된 42균주 C. jejuni에 대한 gyrA 유전자의 염기서열을 분석한 결과 ciprofloxacin에 내성을 가진 40균주에서 아미노산 서열이 ACA (트레오닌)에서 ATA (이소루신)으로 돌연변이 되어있음을 확인하였다. 하지만 대체 치료제인 erythromycin과 azithromycin에 대해서는 97.6% (41균주) 감수성을 보였다. 또한 C. jejuni의 혈청형을 분석한 결과 4가지 타입으로 분류되었는데, HS2(B), HS3(C), HS4(D), HS19(O)로 확인되었다. 도내에서 분리한 C. jejuni 42균주의 유전형을 rep-PCR과 PFGE로 확인한 결과 PFGE에 의해서는 12 cluster, rep-PCR에 의해서는 11 cluster의 유전형으로 구분되었다. Campylobacter jejuni is one of important food-borne pathogens causing human gastroenteritis. We isolated 42 strains of C. jejuni from diarrhea patients and 4 food-poisoning outbreaks in 2010, Gyeonggi-do. In this study, 42 strains were tested for genetic characteristics, the serotype distribution and antimicrobial resistant rate. The presence of hipO (100%), cdtB (100%), and mutated gyrA (95.2%) genes was detected in C. jejuni by polymerase chain reaction (PCR). Detection of mutated gyrA gene correlated with ciprofloxacin resistance. Forty isolates had mutated gyrA gene and were actually resistant to ciprofloxacin. Furthermore, comparing the gyrA DNA sequence data, ciprofloxacin-resistant isolates had a mutation of the DNA sequence from ACA (threonine) to ATA (isoleucine). But 41 strains (97.6%) of patient isolates were susceptible to erythromycin and azithromycin. A total of 35.7% among 42 C. jejuni isolates were identified into 4 different serotypes. The serotype distribution of C. jejuni strains were shown to be HS2(B), HS3(C), HS4(D), HS19(O). To investigate the genotypes of C. jejuni isolated in Gyeonggi province, repetitive sequence polymerase chain reaction (rep-PCR) analysis and SmaI-digested pulsed-filed gel electrophoresis (PFGE) profile analysis were performed. From the PFGE analysis of 42 C. jejuni strains, 12 clusters of PFGE profile were obtained. On the other hand, 11 clusters of rep-PCR profile were obtained from 42 strains of C. jejuni.

경기 북부 내 직거래 농산물의 잔류농약 실태조사 및 위해성 평가

임정화,박포현,임부건,유경신,강민성,송서현,강남희,유나영,김정은,강충원,김윤호,서정화,최옥경,윤미혜 한국식품위생안전성학회 2020 한국식품위생안전성학회지 Vol.35 No.3

In this study, we investigated pesticide residues in 207 agricultural products distributed by direct trade in the northern area of Gyeonggi Province. A total of 94 general agricultural products and 113 eco-friendly agricultural products collected from local grocers and cooperative stores were analyzed by multiresidue method for 263 pesticides using GC (gas chromatography)/ECD (electron capture detector), GC/NPD (nitrogen phosphorus detector), GC-MS/MS (tandem mass spectrometry), LC (liquid chromatography)/PDA (photodiode array detector), LC/FLD (fluorescence detector), LCMS/MS. All samples showing pesticide residues were general agricultural products collected from local food stores. The pesticide residue levels of 14 samples (6.8%) were below the maximum residue limits (MRLs) and one of them (0.5%) exceeded the MRLs. Sixteen pesticides were detected from samples of the following produce items: spinach, young cabbage, perilla leaves, mallow, cucumber, chives and water dropwort. The safety of the detected pesticides was assessed by monitoring the daily intake estimate (EDI) and the daily intake allowance (ADI) based on the amount of pesticides detected. The ADI percentage range (the ratio of EDI to ADI) was 0.0134-61.6259% and there was no health risk connected with consuming agricultural products in which pesticide residues were detected.

2014년 경기지역에서 발생한 해외유입 홍역 바이러스의 분자역학적 특성 분석

윤희정,박포현,황선일,허정원,남수정,용금찬,윤미혜,Yun, Hee-Jeong,Park, Po-Hyun,Hwang, Sun-Il,Huh, Jeong-Weon,Nam, Soo-Jung,Yong, Kum-Chan,Yoon, Mi-Hye 한국미생물학회 2017 미생물학회지 Vol.53 No.1

Measles virus is a highly contagious, exanthematic virus, preventable by the use of an effective live-attenuated vaccine. However, measles virus remains endemic in many area of the world causing nearly 200,000 deaths per year and still a major cause of child mortality, mostly in developing countries. In March 2014, Republic of Korea was certified as a 'national measles elimination' by the WHO as a result of a high-quality case-based surveillance system and population immunity, which was achieved by a high vaccination rate (>95.0% since 1996). But, since the beginning of 2014, the Gyeonggi province has experienced a resurgence of measles cases. In this study, we investigated the characteristics of measles viruses isolated from confirmed measles in Gyeonggi province during January 1, 2014 ~ July 31, 2014, 60 isolates were obtained from 72 confirmed measles specimens. Genotypic distributions and genetic diversities of isolated measles virus were analyzed by sequencing of nucleoprotein (N) gene. 58 (96.7%) imported cases were identified. The predominant genotype was B3, which reflects the circulating measles virus in adjacent countries. The sequences of nucleoprotein (N) gene of isolated MeV were showed that the strains characterized showed the highest degree of identity (99%) with the Philippine related strains in 2013-2014. Therefore, infected traveler returning from the Philippines transmitted secondary infection in Korea. 홍역바이러스(Measles virus)는 전염성이 매우 높은 발진성 열병을 일으키는 바이러스이며, 약독화된 생백신 접종으로 충분히 예방할 수 있다. 그러나 대부분의 개발도상국에서 어린아이들의 주요 사망원인이며 해마다 20만명이 사망하는 풍토병이다. 우리나라는 1996년 이후부터 국가적으로 시행된 정기예방접종으로 인한 높은 백신 접종률(>95.0%)로 획득된 집단면역과 고도의 기술적 감시체계의 결과로 홍역이 퇴치되었으며, 2014년 3월 WHO로부터 '홍역퇴치국가'로 인증 받았다. 그러나 2014년 초부터 경기도에서 홍역환자가 다시 발생하였다. 본 연구에서는 2014년 1월부터 2014년 7월까지 경기도에서 발생한 홍역 확진 환자를 대상으로 홍역바이러스의 유행양상 및 유전형 분포양상을 분석하였다. 72건의 홍역바이러스 양성검체에서 60주의 홍역바이러스를 분리하였고, nucleoprotein(N) 유전자의 염기서열 분석을 통하여 유전형의 확인 및 유전자변이를 분석하였다. 총 60건의 홍역 확진검체를 분석한 결과 58건(96.7%)에서 B3 genotype을 확인하였다. 또한 nucleoprotein(N) 유전자 부위의 유전자 변이 분석결과, 경기도내 분리주들이 2013년에서 2014년 필리핀에서 유행한 홍역 분리주들과 99% 일치하였다. 따라서 필리핀 등에서 감염된 후 국내에 유입되어 2차 감염이 된 것으로 사료된다.

2014-2015년 경기지역 노로바이러스성 식중독의 분자역학적 특성분석

남수정,박포현,방선재,허정원,윤희정,박광희,윤미혜,Nam, Soo-Jung,Park, Po-Hyun,Bang, Sun-Jae,Huh, Jeong-Weon,Yun, Hee-Jeong,Park, Kwang-Hee,Yoon, Mi-Hye 한국미생물학회 2018 미생물학회지 Vol.54 No.1

Norovirus infection is a leading cause of nonbacterial gastroenteritis outbreaks. New variants of GII.4 have emerged approximately every 2~3 years and have caused norovirus gastroenteritis pandemics globally. In this study, analysis and molecular genetic characteristics of the norovirus gastroenteritis outbreaks 2,917 samples in Gyeonggi-Do from 2014 to 2015. As a result, 247 samples out of 2,917 samples are positive for norovirus. Norovirus molecular genetic characteristics of the GI 8 types (GI-1, 2, 3, 4, 5, 6, 12, 14), GII 10 types (GII - 2, 3, 4, 5, 6, 11, 12, 14, 16, 17). Genome sequences of isolated noroviruses were similar to those of new GII.17 Kawasaki 2014 variants with 96.6 identity, suggesting that these viruses were imported from overseas. 44% of virus incidence was originated from school meal service. Therefore, a continuous monitoring and school sanitation should be required for preventing a massive virus outbreak. 노로바이러스는 식중독을 유발하는 주요 병원체이며, 최근 GII.4 변이주의 출현으로 전세계적으로 2~3년 주기로 유행하고 있다. 본 연구에서는 2014년부터 2015년까지 2년간 경기도 내 집단식중독 검체 2,917건을 대상으로 노로바이러스성 식중독 발생양상을 파악하고 분자유전학적 특성을 분석하였다. 검사결과 노로바이러스가 247건으로 가장 많았으며, 분자유전학적 특성은 GI 형에서 8종(GI-1, 2, 3, 4, 5, 6, 12, 14), GII형에서 10종(GII-2, 3, 4, 5, 6, 11, 12, 14, 16, 17)으로 다양하게 분포하고 있는 것으로 나타났다. 2015년 초 집단발생한 노로바이러스 35건에 대한 유전계통학적 분석결과 GII-17 kawasaki 2014형으로 알려진 분리주와 96.6% 이상 일치하여 새로운 변이주에 의한 유행이었음을 확인하였으며, 학교 등 급식에 의한 비율이 44%로 단체급식 위생에 주의하는 한편 발생 원인을 파악하여, 대규모 식중독 유행에 대비하여야 할 것으로 판단된다.

경기도내 유통 생즙 원료 농산물의 잔류농약 실태조사 및 위해성 평가

유경신,박포현,김기유,임부건,강민성,이유진,강충원,김윤호,이선영,서정화,박용배,윤미혜 한국식품위생안전성학회 2018 한국식품위생안전성학회지 Vol.33 No.5

본 연구는 2017년 1월부터 9월까지 생즙 원료 농산물 12품목 297건에 대한 잔류농약의 실태를 조사한 것이다. GC/ECD, GC/NPD, TOF/MS, LC/PDA, LC/FLD, LC/ MSMS를 이용하여 다종농약다성분 분석법으로 220종의 잔류농약을 분석하였고, 검사결과 14건(4.7%)에서 9종의 농약성분이 검출되었다. 검출된 농약은 클로로피리포스, 사이플루페나미드, 클로로탈로닐, 펜디메탈린, 아족시스트 로빈, 피리달릴, 이마잘릴, 플루디옥소닐, 프로사이미돈이 였으며, 검출된 농산물은 감귤, 당근, 사과, 셀러리, 치커리, 오렌지, 포도였다. 농약 검출량을 바탕으로 일일섭취 추정량(EDI)과 일일섭취허용량(ADI)을 이용하여 위해성평가를 하였으며, %ADI값의 범위는 0.0011~6.7087%로 안 전한 수준이었다. This study was conducted to monitor the current status of pesticide residues in a total of 297 agricultural products for raw juice from January 2017 to September 2017. Pesticide residues in samples were analyzed by multiresidue method for the determination of multiclass pesticide with an aim to detect 220 pesticides using GC/ECD, GC/NPD, TOF/MS, LC/PDA, LC/FLD, and LC/MS-MS. The analysis revealed the presence of nine pesticides in 14 samples. The detected pesticides were chlorpyrifos, cyflufenamid, chlorothalonil, pendimethalin, azoxystrobin, pyridalyl, imazalil, fludioxonil and procymidone. The kinds of detected crops were mandarin, carrot, apple, celery, chicory, orange and grape. Risk assessment evaluated human health exposure with the ratio of EDI (Estimated daily intake) to ADI (Acceptable daily intake) of pesticides detected and the range of %ADI (the ratio of EDI to ADI) values was 0.0011~6.7087% and safety level.

쑥갓 중 bifenthrin과 chlorothalonil의 경시적 잔류량 변화 및 생물학적 반감기

강민성,박포현,김기유,임부건,유경신,이유진,임정화,강충원,김윤호,이선영,서정화,박용배,윤미혜 한국식품위생안전성학회 2019 한국식품위생안전성학회지 Vol.34 No.2

This study investigated the residual characteristics of bifenthrin and chlorothalonil in crown daisy and suggested pre-harvest residue limits (PHRLs) based on their dissipation patterns and biological half-lives. The samples for residue analysis were harvested at 0 (3 hr), 1, 3, 5, 7, 9, 11, 13, 15, 18, 22 and 26 days after treatment, and analyzed by GC/ µ-ECD and TOF/MS. The limit of quantitation (LOQs) of bifenthrin and chlorothalonil were 0.0046 mg/kg and 0.0007 mg/ kg, respectively. Recoveries ranged from 88.67 ± 7.97% and 99.90 ± 16.03%, showing that this method is appropriate for the analysis of the pesticide residues in crown daisy. Being well within first order kinetics, the biological half-lives of the pesticide residues in crown daisy were 9.63 days for bifenthrin and 6.54 days for chlorothalonil. The PHRLs of bifenthrin and chlorothalonil were recommended as 11.70 mg/kg and 24.10 mg/kg for 26 days before harvest, respectively.

구두발표 / O-1 : 침출차 종이 포장재중 Phthalate esters 화합물의 분석

김기철,김양희,박포현,엄미나,고환욱,조규홍,김세진 한국분석과학회 1999 학술대회논문집 Vol.- No.-

근적외선 분광광도법을 이용한 PVC포장재 중 DEHP 정량법에 관한 연구

김재관,윤미혜,박포현,김기철 대한환경위생공학회 2002 대한환경위생공학회지 Vol.17 No.4

어린이집 유아 손의 미생물학적 위해 평가 및 손 씻기 교육의 효과

김중범,허은선,강석호,김대환,도영숙,박포현,박용배,윤미혜,이정복 한국식품위생안전성학회 2012 한국식품위생안전성학회지 Vol.27 No.1

This study was conducted to evaluate the microbiological hazard on nursery school children's hands and to investigate the reduction effect of hand washing education. A total of 59 nursery school children's hands were tested. The average number of total aerobic bacteria was 3.72 ± 0.38 log CFU/hand. Five children's hands(2male and 3 female) were positive(14.3%) for the coliform bacteria. These results showed that hand washing education are required repetitively. Among the pathogenic bacteria tested in this study, Staphylococcus aureus and Bacillus cereus were detected in 9(25.7%) and 16(45.7%) out of 35 their hands, respectively. Twelve out of sixteen B. cereus isolates(70.0%) produced enterotoxin. The results indicate that the hand hygiene of nursery school children needs to be improved. Comparing before and after hand washing in educated and non-educated group, the reduction effect of total aerobic bacteria on their hands was 0.42 and 0.60 log CFU/hand, respectively. The educated group showed 0.18log CFU/hand higher reduction effect than non-educated group but microorganism did not eliminate perfectly. From the results, using a hand sanitizer after washing with soap and the continuous hand washing education are required to control the contaminated bacteria on nursery school children's hands.