능성어(Hyporthodus septemfasicatus)의 산업적 양식을 위한 수정란 대량생산

박충국 ( Chung-kug Park ) 한국수산과학회(구 한국수산학회) 2021 한국수산과학회지 Vol.54 No.1

The mass production of fertilized eggs of the convict grouper Hyporthodus septemfasciatus was studied from 2013 to 2020 for industrial aquaculture. The experiment was divided into two groups. Group 1 broodstock was raised from wild-caught fry and used from 2013 to 2020. Group 2 broodstock was raised from artificially propagated fry and used from 2019 to 2020. Males used to collect sperm for artificial insemination weighed more than 7 kg. The effects of various hormones on artificial ovulation were investigated from 2013 onward. Among these, luteinizing hormonereleasing hormone analogue (LHRHa) at 100 μg/kg body weight showed the most effective results and was used for artificial egg collection from 2014 onward. In Group 1, the average total egg production per year, average egg production per individual, fertilization rate, and hatching rate were 26,143 mL, 609.7 mL, 93.3%, and 91.8%, respectively, and in Group 2, were 2,750 mL, 316.5 mL, 92.1%, and 90.4%, respectively. Based on these results, we showed that a large number of fertilized eggs for artificial seeding could be produced consistently. Moreover, the mass production of fertilized eggs in Group 2 establishes a foundation for the complete aquaculture cycle of H. septemfasciatus.

남해안 여자만에 서식하는 짱뚱어 Boleophthalmus pectinirostris의 난발생 및 자치어 형태발달

박충국 ( Chung-kug Park ),황선영 ( Seon-yeong Hwang ),김대홍 ( Dae-hong Kim ),허승준 ( Seung-jun Heo ),박재민 ( Jae-min Park ) 한국어류학회 2024 韓國魚類學會誌 Vol.36 No.1

이 연구는 남해안 여자만에 서식하는 짱뚱어의 초기생활사를 규명하고 그 결과를 같은 망둑어과 어류들과 차이점을 비교하였다. 연구에 사용된 어미는 2015년 6월 전남 벌교읍 갯벌에서 맨손으로 포획하였다. 산란량은 411~11,688개였고, 난은 짧은 타원형으로 크기는 1.40×0.72 mm였다. 수온 25~27℃에서 부화시간은 91시간 35분이 소요되었다. 부화 직후 난황자어는 전장 3.02~3.31 (평균 3.17±0.08, n=30)mm로 난황을 갖고 있었고, 먹이 섭취는 하지 않았다. 부화 후 4일째에는 전장 3.31~3.52 (평균 3.43±0.07, n=30) mm로 입과 항문이 열리면서 전기자어기로 이행하였고, 먹이를 섭취하였다. 부화 후 14일째 전장 5.06~5.25 (평균 5.16±0.06, n=30)mm로 척추 말단 끝부분이 45°로 완전히 휘어지면서 후기자어기로 이행하였다. 부화 후 41일째 전장 14.3~16.8 (평균 15.4±0.85 mm, n=30)로 각 지느러미 기조수는 제1등지느러미 5개, 제2등지느러미 26~27개, 뒷지느러미 24~27개, 배지느러미 6개로 정수에 달하면서 치어기로 이행하였다. 연구 결과 후기자어는 별 모양의 흑색소포가 가슴지느러미 앞쪽부터 꼬리지느러미 기저까지 침착되어 있어 다른 망둑어과 어류 후기자어들과 형태적인 차이가 구분되었다. This study investigated the early life history of the Boleophthalmus pectinirostris living in the southern coastal Yeoja-man and compared the results with the same Gobiidae fishes. The brood stork used in the study were captured with bare hands in the tidal flats of Beolgyo-eup, Jeollanam-do, in June 2015. The amount of spawning was 411~11,688, and the eggs were short oval and the size was 1.40×0.72 mm. The time of hatched took 91 hours and 35 minutes at a water temperature of 25~27℃. Newly hatching larvae, the yolk sac had a total length of 3.02~3.31 (average 3.17±0.08, n=30) mm and did not eat rotifer. 4 days after hatching, the total length was 3.31~3.52 (3.43±0.07, n=30) mm, and as the mouth and anus opened, the fish transitioned to the preflexion larvae and fed. 14 days after hatching, the total length was 5.06~5.25 (5.16±0.06, n=30) mm, and the distal end of the vertebra was completely bent at 45° and the transitioned to the postflexion larvae. 41 days after hatching, the total length was 14.3~16.8 (15.4±0.85 mm, n=30), and the number of fins reached an integer of 5 first dorsal fins, 26~27 second dorsal fins, 24~27 anal fins, and 6 ventral fins, and the transitioned to the juveniles. As a result of the study, star-shaped melanophore were deposited from the front of the pectoral fin to the base of the caudal fin, which distinguished them in form from other postflexion larvae of Gobiidae fishes.

호르몬 처리에 의한 붉바리 Epinephelus akaara의 배란유도

박종연,조재권,박충국,한경호,홍창기,Park, Jong-Youn,Cho, Jae-Kwon,Park, Chung-Kug,Han, Kyeong-Ho,Hong, Chang-Gi 한국해양생명과학회 2019 한국해양생명과학회지 Vol.4 No.2

본 연구는 붉바리 배란유도를 위해 다양한 호르몬과(ovaprim, pimozide, LHRHa, HCG)과 LHRHa의 농도별 효과를 조사하였다. LHRHa는 50 ㎍kg^-1, 100 ㎍kg^-1, 150 ㎍kg^-1, 200 ㎍kg^-1의 농도로 처리하였다. 호르몬은 등 근육에 주사하였으며, 조사 결과 LHRHa 단독, 그리고 LHRHa와 Pimozide를 혼합하여 투여했을 때 가장 효과적이었으며, 두 실험구 사이의 차이는 거의 없는 것으로 나타났다. 따라서 LHRHa를 단독으로 사용하는 것이 가장 효율적인 것으로 판단된다. 그리고 LHRHa를 다양한 농도로 처리하여 배란유도 효과를 조사한 결과 100 ㎍kg^-1의 농도로 투여했을 때 가장 우수한 것으로 조사되었다. This study aims to investigate how ovulation induction of red spotted grouper, Epinephelus akaara varies when treated with different hormonal substances such as ovaprim, pimozide, LHRHa, and HCG. As for LHRHa, we injected it at four different concentrations: 50 ㎍kg^-1, 100 ㎍kg^-1, 150 ㎍kg^-1, and 200 ㎍kg^-1. All hormonal substances including LHRHa were injected into the back muscles. Buoyant, embryonic survival, fertilization, and hatching rate were the highest in LHRHa and LHRHa + pimozide group. When LHRHa was injected at different concentrations, buoyancy and fertilization rate were the highest in 100 ㎍kg^-1 group. Embryonic survival and hatching rate were the highest in 150 ㎍kg^-1. Based on these experimental results, we concluded that LHRHa at the concentration of 100 ㎍kg^-1 is the most efficient for the ovulation induction in this fish species.

황점볼락 Sebastes oblongus 인공종묘의 생식소 발달과 성비

곽은주 ( Eun Joo Kwak ),이경우 ( Kyung Wo Lee ),최낙현 ( Nak Hyun Choi ),박충국 ( Chung Kug Park ),한경호 ( Kyeong Ho Han ),이원교 ( Won Kyo Lee ),( Seok Woo Yang ) 한국수산과학회 2006 한국수산과학회지 Vol.39 No.3

수온과 염분이 능성어(Epinephelus septemfasciatus)의난 발생 및 자어에 미치는 영향

조재권 ( Jae Kwon Cho ),홍창기 ( Chang Gi Hong ),박종연 ( Jong Youn Park ),손맹현 ( Maeng Hyun Son ),박충국 ( Chung Kug Park ),박재민 ( Jae Min Park ) 한국어류학회 2015 韓國魚類學會誌 Vol.27 No.1

본 연구는 해상가두리에서 사육한 능성어의 수정란을 가지고 난 발생 및 자어에 미치는 수온과 염분의 영향에 대하여 연구하였다. 수정란은 유백색의 구형으로 분리부성란이었고, 1개의 유구를 가지고 있었으며, 난 발생 과정 동안의 수온은 21.5~23.5℃(평균 22.0±0.05℃), 염분은 32.0~33.0psu (평균 32.5±0.05 psu) 범위였다. 각 수온조건별로 부화에 소요된 시간은 15℃에서 29시간, 20℃에서 27시간, 25℃에서 24시간, 30℃에서 17시간, 35℃에서 15시간이 소요되었다. 염분별 부화율은 15 psu에서 80.0%, 20 psu에서 81.8%, 25 psu에서 86.4%, 30 psu에서 89.1%, 35 psu에서 79.1%로25~30 psu에서 가장 부화율이 높은 것으로 나타났다. 기형율은 저염분 구간인 15 psu (20.0%)와 20 psu (18.2%)보다35 psu에서 기형율이 20.9%로 가장 높았다. 각 발생 단계별소요시간 (t : hour)과 수온(T : ℃)과의 관계는 직선함수식의 관계를 가지는 것으로 나타났다. 초기발생에 있어 난 발생이 진행되지 않는 생물학적 영도는 평균 5.4℃로 추정되었다. We studied the effects of temperature and salinity on the egg development and larvae of sevenband grouper Epinephelus septemfasciatus under sea cage culturing condition. In regard to rearing environment, the water temperature is 21.5~23.5℃ (mean 22.0±0.05℃) and the salinity is32.0~33.0 psu (mean 32.5±0.05 psu). The time of egg development was positively proportional towater temperature with 29 hrs, 27 hrs, 24 hrs, 17 hrs, 15 hrs after fertilization in 15℃, 20℃, 25℃, 30℃, 35℃ respectively. Each of the salinity hatching rate was 80% in the section of 15 psu, 81.8% in the section of 25 psu, 89.1% in the section of 30 psu, and 79.1% in the section of 35 psu. All things considered, the highest hatching rate appeared in the range of the section of 25~30 psu. Abnormalities of low salinity section, such as 15 psu (20.0%) and 20 psu (18.2%), was lower than the abnormalities of35 psu that belongs to high salt section. The relation between the time of egg development (t : hour)and water temperature (T : ℃) was represented by the mathematical formulae. The mean biological minimum temperature was 5.4℃.

한국 특산종 자가사리(Liobagrus mediadiposalis)의 산란행동 및 초기 생활사

최낙현 ( Nak Hyun Choi ),서원일 ( Won Il Seo ),김춘철 ( Chun Chel Kim ),박충국 ( Chung Kug Park ),허승준 ( Seung Joon Heo ),윤성민 ( Seung Min Yoon ),한경호 ( Kyeong Ho Han ),이원교 ( Won Kyo Lee ) 한국수산학회 2008 한국수산과학회지 Vol.41 No.6

고등어(Scomber japonicus ) 치어의 마취제로서 Clove oil, MS-222 및 2-Phenoxyethanol의 평가

한석중 ( Seock Jung Han ),김경민 ( Kyong Min Kim ),최낙중 ( Nack Jung Choi ),구준호 ( Jun Ho Koo ),박충국 ( Chung Kug Park ),이원교 ( Won Gyo Lee ),지승철 ( Seung Chul Ji ) 한국수산과학회(구 한국수산학회) 2011 한국수산과학회지 Vol.44 No.6

The efficiency of clove oil, MS-222, and 2-phenoxyethanol was evaluated as anesthetics in juvenile Scomber japonicus . Stage A5 of anesthesia was assumed to be sufficient for conducting routine aquaculture procedures in less than 3 min, with recovery (stage R5) in less than 5 min. The lowest effective doses of the three anesthetics were 50 mg L-1 clove oil (anesthetic time of 71.3 s and recovery time of 167.0 s), 100 mg L-1 MS-222 (anesthetic time of 70.7 s and recovery time of 115.7 s), and 400 mg L-1 2-phenoxyethanol (anesthetic time of 86.7 s and recovery time of 95.0 s). Anesthetic times decreased with increasing doses for all three anesthetic agents, and fish anesthetized with clove oil exhibited the longest recovery times. After 30 min, the highest plasma cortisol and lactate levels were detected with the use of clove oil, whereas the lowest values were observed with 2-phenoxyethanol. In addition, high glucose levels were maintained during recovery with clove oil, but the treatments did not significantly differ. The most effective of the three anesthetic agents was 2-phenoxyethanol, although all were considered acceptable for use in cultures of juvenile Scomber japonicus