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      • KCI등재

        P/M Fecralloy의 성형성 및 전기저항특성 향상에 관한 연구

        박진우,고병현,정우영,박동규,안인섭,Park, Jin-Woo,Ko, Byung-Hyun,Jung, Woo-Young,Park, Dong-Kyu,Ahn, In-Shup 한국분말야금학회 2016 한국분말야금학회지 Vol.23 No.6

        The Fe-Cr-Al alloy system shows an excellent heat resistance because of the formation of an $Al_2O_3$ film on the metal surface in an oxidizing atmosphere at high temperatures up to $1400^{\circ}C$. The Fecralloy needs an additive that can act as a binder because of its bad compactability. In this study, the green compacts of STS434L and Al powder added to Fecralloy are oxidized at $950^{\circ}C$ for up to 210 h. Fecralloy and Al is mixed by two types of ball milling. One is vented to air and the other was performed in a sealed jar. In the case of Al addition, there are no significant changes in the electrical resistance. Before the oxidation test, Al oxides are present in the Fecralloy surface, as determined from the energy dispersive spectroscopy results. The addition of Al improves the compactability because of an increased density, and the addition of STS434L increases the electrical resistivity by forming a composite oxide.

      • KCI등재

        타당성 평가가 보완된 모델 운용상의 전투실험 모의분석 절차 연구

        박진우,김능진,강성진,서혁,Park, Jin-Woo,Kim, Nung-Jin,Kang, Sung-Jin,Soo, Hyuk 한국시뮬레이션학회 2010 한국시뮬레이션학회 논문지 Vol.19 No.4

        현대는 산업사회에서 정보화 사회로 변화되고 있다. 이에 따라 전쟁 양상도 정보전, 네트워크 중심전, 장거리 정밀전, 로봇전으로 발전되어 고도의 첨단 과학군으로 변화를 시도하고 있다. 이러한 전투발전을 위한 전력창출을 위해 중요시 되고 있는 것이 전투실험이다. 전투실험은 LVC를 통한 입체적인 평가체계를 사용하는 것이 바람직하나, 비용 시간 장소 자원의 제한으로 M&S(Modeling & Simulation)를 통한 모의분석을 주로 활용한다. 하지만 모델 개발을 위한 평가절차에 대한 연구는 많이 이루어지고 있지만, 전투실험에 대한 모델운용상의 모의분석절차의 정립이 미흡하다. 본 연구에서는 전투실험간 모델운용의 타당성 평가체계(VV&A : Verification Validation & Accreditation)가 보완된 모의분석 방법과 절차를 정립하였고, "미래향토사단 군수부대편성의 적절성 평가"를 위해 지상무기효과분석모델(AWAM : Army Weapon Analysis Model)을 통한 후방 지역작전 모의분석 사례를 제시하였다. 본 연구는 신뢰성있는 평가체계를 바탕을 둔 효과적인 모의분석방법을 제시하여 전투실험을 위해 크게 기여할 것으로 판단된다. Currently, our society has been changed from the industrial society to the information society. As the war progresses to Information Warfare, Network-Centric Warfare, Long-Range Precision Engagement and Robot Warfare, the military should advance to High-tech Scientific force. For this creation of the war potential, it is regarded as the warfighting experiment is a critical method. Surely it is rational that LVC(Live Virtual Constructive simulation) is desirable to make the warfighting experiment. But because it is limited by the cost, the time, the place and the resource, the constructive simulation(M&S : Modeling&Simulation) is a good tool to solve those problems. There are some studies about the evaluation process for developing the model, but it is unsatisfying in the process of the constructive simulations' operation. This study focuses on the way of constructive simulation operation, which is supplied with the evaluation process(VV&A : Verification Validation & Accreditation). We introduce the example of the rear area operation simulation for "appropriateness evaluation to the organization of logistic corps" by the AWAM(Army Weapon Analysis Model). This study presents the effective methods of the constructive simulations, which is based on the reliable evaluation process, so it will contribute to the warfighting experiments.

      • KCI등재

        P/M Fecralloy 성형체의 고온산화 및 전기저항 안정성에 미치는 SiO<sub>2</sub> 첨가 효과

        박진우,옥진욱,정우영,박동규,안인섭,Park, Jin-Woo,Ok, Jin-Uk,Jung, Woo-young,Park, Dong-kyu,Ahn, In-Shup 한국분말야금학회 2017 한국분말야금학회지 Vol.24 No.4

        A metallic oxide layer of a heat-resistant element contributes to the high-temperature oxidation resistance by delaying the oxidation and has a positive effect on the increase in electrical resistivity. In this study, green compacts of Fecralloy powder mixed with amorphous and crystalline silica are oxidized at $950^{\circ}C$ for up to 210 h in order to evaluate the effect of metal oxide on the oxidation and electrical resistivity. The weight change ratio increases as per a parabolic law, and the increase is larger than that observed for Fecralloy owing to the formation of Fe-Si, Fe-Cr composite oxide, and $Al_2O_3$ upon the addition of Si oxide. Si oxides promote the formation of $Al_2O_3$ and Cr oxide at the grain boundary, and obstruct neck formation and the growth of Fecralloy particles to ensure stable electrical resistivity.

      • SCIESCOPUSKCI등재

        Cellular activities of osteoblast-like cells on alkali-treated titanium surface

        박진우,이덕혜,여신일,박광범,최석규,서조영,Park, Jin-Woo,Lee, Deog-Hye,Yeo, Shin-Il,Park, Kwang-Bum,Choi, Seok-Kyu,Suh, Jo-Young The Korean Academy of Periodontoloy 2007 Journal of Periodontal & Implant Science Vol.37 No.2

        임플란트와 골 사이의 결합력을 증가시키기 위하여 타이타늄 표면에 변화를 주기위한 많은 연구들이 진행되고 있다. 타이타늄의 표면 구조나 미세 지지도의 변화가 임플란트에 대한 세포의 반응에 영향을 미치며, 골아 유사세포는 표면 조도가 높은 타이타늄 표면에 더 잘 부착하며, 세포외 기질의 합성과 광물화 결정이 더 잘 일어난다고 알려져 있다. 그러나 대부분의 연구들은 마이크로 단위의 미세 지지도에 대한 연구들이고 나노 단위의 미세 지지도에 대한 연구들은 미미하다. 이에 본 연구에서는 ROS 17/2.8 cell line을 이용하여 기계적 처리만한 군을 대조군으로 하여 blasting 처리한 마이크로 단위의 미세 지지도 표면과 알칼리 처리된 나노 단위의 미세 지지도 표면에 대한 골아 유사세포의 세포 부착양상, 증식 그리고 골아 유사세포의 표식인자 발현양상 등을 상호 비교하여 골아 유사세포에 미치는 영향을 관찰하고자 하였다. SEM을 이용한 미세 지지도 관찰에서 알칼리 처리군에서는 약 200mm의 초미세 다공성의 양상을 나타내었고, blasting 처리한 군에서는 $10\;{\mu}m$ 이하의 움푹 파인 양상을 보였다. 표면조도 측정에 있어서는 blasting 처리한 군에서 기계적 처리와 알칼리 처리된 군보다 더 높은 표면 조도를 보였으며 이는 통계학적으로 유의한 차이를 나타내었다 (p<0.01). 표면결정성 분석에서는 알칼리처리 군에서 anatase와 rutile결정형이 보였으나, blasting 처리한 군과 기계적 처리 군에서는 관찰되지 않았다. 골아 유사세포 1시간 배양 후의 전자현미경 관찰에서 모든 군의 세포는 부착 및 전개 과정을 보였고, 3시간 배양에서는 모든 군의 세포가 더 많이 전개되었으나, blasting 처리한 군과 알칼리처리 군에서 세포가 다소 더 불규칙한 형태를 나타내었다. 24시간 배양에서는 모든 군의 세포에서 완전히 전개가 일어난 양상을 보였다. 1, 4, 7일간 세포배양 후 세포활성을 평가하기 위한 MTT assay에서는 모든 군에서 시간이 증가함에 따라 세포수가 증가하였으며, 1일째에 blasting 처리한 군과 알칼리처리 군에서 기계적 처리 군에 비해 세포활성도가 통계학적으로 유의한 증가를 보였다(p<0.01). 골아 유사세포 표식인자인 osteopontin, alkaline phosphatase, ${\alpha}\;1(1)$ collagen의 유전자 발현양상을 관찰해 본 결과, osteopontin, alkaline phosphatase, ${\alpha}\;1(1)$ collagen의 유전자 발현양상이 세 군 모두에서 유의한 차이는 관찰할 수 없었으나, blasting 처리한 군과 알칼리처리 군에서 기계적 처리 군에 비해 유전자 발현양상이 다소 증가하는 경향을 보였다. 결론적으로 blasting 처리한 마이크로 단위의 미세 지지도 표면과 알칼리 처리된 나노 단위의 미세 지지도 표면이 기계적 처리 군에 비해 골아 유사세포의 기능을 촉진시키나, 알칼리 처리된 나노 단위의 미세 지지도 표면은 blasting 처리한 마이크로 단위의 미세 지지도 표면이 골아 유사세포의 기능에 미치는 영향을 압도하지는 않는 것으로 사료된다. To improve osseointegration at the boneto-implant interface, several studies have been carried out to modify titanium surface. Variations in surface texture or microtopography may affect the cellular response to an implant. Osteoblast-like cells attach more readily to a rougher titanium surface, and synthesis of extracellular matrix and subsequent mineralization were found to be enhanced on rough or porous coated titanium. However, regarding the effect of roughened surface by physical and mechanical methods, most studies carried out on the reactions of cells to micrometric topography, little work has been performed on the reaction of cells to nanotopography. The purpose of this study was to examme the response of osteoblast-like cell cultured on blasted surfaces and alkali treated surfaces, and to evaluate the influence of surface texture or submicro-scaled surface topography on the cell attachment, cell proliferation and the gene expression of osteoblastic phenotype using ROS 17/2.8 cell lines. In scanning electron micrographs, the blasted, alkali treated and machined surfaces demonstrated microscopic differences in the surface topography. The specimens of alkali treatment had a submicro-scaled porous sur-face with pore size about 200 nm. The blasted surfaces showed irregularities in morphology with small(<10 ${\mu}m$) depression and indentation among flatter-appearing areas of various sizes. Based on profilometry, the blasted surfaces was significantly rougher than the machined and the alkali treated surfaces (p<O.01). On the x-ray diffraction analysis, anatase and rutile($TiO_2$) were observed on alkali treated surfaces, whereas not observed on machined and blasted surfaces. The attachment morphology of cells according to time was observed by the scanning electron microscope. After 1 hour incubation, the cells were in the process of adhesion and spreading on the prepared surfaces. After 3 hours, the cells on all prepared surfaces were further spreaded and flattened, however on the blasted and alkali treated surfaces, the cells exhibited slightly irregular shapes and some gaps or spaces were seen. After 24 hours incubation, most cells of the all groups had a flattened and polygonal shape, but the cells were more spreaded on the machined surfaces than the blasted and alkali treated surfaces. The MTT assay indicated the increase on machined, alkali treated and blasted surfaces according to time, and the alkali treated and blasted surfaces showed significantly increased in optical density comparing with machined surfaces at 1 day (p<0.01). Gene expression study showed that mRNA expression level of ${\alpha}\;1(I)$ collagen, alkaline phosphatase and osteopontin of the osteoblast-like cells showed a tendency to be higher on blasted and alkali treated surfaces than on the machined surfaces, although no siginificant difference in the mRNA expression level of ${\alpha}\;1(I)$ collagen, alkaline phosphatase and osteopontin was observed among all groups. In conclusion, we suggest that submicroscaled surfaces on osteoblast-like cell response do not over-ride the one of the surface with micro-scaled topography produced by blasting method, although the microscaled and submicro-scaled surfaces can accelerate osteogenic cell attachment and function compared with the machined surfaces.

      • SCIESCOPUSKCI등재

        ($XiVE^{(R)}$ 임프란트의 성공률에 관한 후향적 연구;임상성적에 관한 조기평가

        박진우,Park, Jin-Woo 대한치주과학회 2005 Journal of Periodontal & Implant Science Vol.35 No.1

        This retrospective study evaluates the clinical performance of the recently introduced $XiVE^{(R)}$ implant(Dentsply-Friadent) with a new macro-design to improve primary stability. A total of 208 $XiVE^{(R)}$ implants (101 in the maxilla and 107 in the mandible) were placed in 71 patients. The average age of the patients was 49 years. Of the 208 implants, 190 (91.3%) were posterior implants and 82 (39.4%) were placed in compromised sites (grafted sites). Clinical and radiographic evaluation were made at second stage surgery for exposure and after functional loading. 192 implants in 64 patients were evaluated at exposure and 146 implants in 50 patients were loaded (average 170 days-loading) and evaluated after functional loading. Of 192 implants available for evaluation before loading, 3 implants failed (early failure) ; 1 before exposure, 1 at exposure and 1 during prosthetic procedure. 2 implants were in the maxilla and 1 was in the mandible. The success rate before loading was 98.4%. After functional loading, no implant failure was occurred in 146 implants evaluated during this period (100% interval success rate). This preliminary data with a new implant showed excellent success rate although the majority of implants evaluated in this study were placed in the posterior region of the jaw and compromised sites.

      • SCIESCOPUSKCI등재

        탈단백 우골의 골이식 대체재로서의 특성에 대한 평가;세 종류의 골 대체재의 기본 특성에 대한 비교분석

        박진우,Park, Jin-Woo 대한치주과학회 2005 Journal of Periodontal & Implant Science Vol.35 No.4

        Bovine bone-derived bone substitutes are widely used for treatment of bone defects in dental and orthopedic regenerative surgery. The purpose of this study was to evaluate the basic characteristics of deproteinized bovine bone mineral as a bone graft substitute. Commercially available products from three different bovine bone minerals-Bio-Oss(GeistlichPharma, Switzerland), BBP(Oscotec. Korea), Osteograf/N-300(Dentsply Friadent Ceramed, USA) - were investigated. They were evaluated by scanning electron microscopy(SEM), energy dispersive X-ray spectrometer(EDS), surface area analysis(BET), and Kjeldahl protein analysis. Cell viability on different products was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) assay. The results of this study indicated that each bone substitute displayed distinct surface properties. Furthermore, Kjeldahl protein analysis indicated that residual crude proteins are present in deproteinized bovine bone mineral. BBP showed relatively large amount of residual protein, which indicated that the possibility of disease transmission can not be safely ruled out. Based on the results of this study, it is suggested that active quality management is strongly needed in operations that involve processing bovine bone tissue for medical use.

      • SCIESCOPUSKCI등재

        PDGF-BB 적용시간이 decalcified dentin에서의 치은섬유아세포의 증식에 대한 효과

        박진우,이재목,서조영,Park, Jin-Woo,Lee, Jae-Mok,Sun, Jo-Young 대한치주과학회 1996 Journal of Periodontal & Implant Science Vol.26 No.4

        Platelet-derived growth factor(PDGF) is one of the polypeptide growth fators. PDGF has been reported as a biological mediator which regulates activities of wound healing process including the cell proliferation, migration and metabolism. Recent studies indicated that demineralized root surface as the primary site for growth factor application has advantages over other application method, especially due to binding capacity of growth factor for exposed matrix component of deminera1ized dentin surface. The purpose of this study is to evaluate optimal application time of PDGF-BB on proliferation of human gingival fibroblasts using deminera1ized dentin surface as primary application site. Human gingival fibroblasts and dentin slabs were prepared from the first premolar tooth extracted for the orthodontic treatment, cells were cultured in DMEM/I0% FBS at the $37^{\circ}C$, 5% CO2 incubator. All of the dentin slabs were preconditioned with Tetracycline HCI(100mg/ml) solution and rinsed in PBS. In the cell proliferation experiment, experimental group was immersed in DMEM containing 10% FBS, 50ng/rnl PDGF-BB during different time(30sec, 1, 2, 4, 8 minutes) and dried. Cells at concentration of $1{\times}10^5$cells/ml were seeded in each culture well which contained dentin slabs and incubated for 6 hours. Then, all of the dentin slabs were moved into new 24 well culture dish and incubated for 24, 48, 72 hours. The cell counting was done by hemocytometer with inverted phase contrast microscope after trypsinization. The results were as follows : The application of PDGF-BB for 1, 2 min slightly increased the number of gingival fibroblasts, and the application of PDGF-BB for 4, 8 min prominently increased the number of gingival fibroblasts. The application of PDGF-BB for 4 min showed maximum proliferation rate of gingival fibroblasts at 24, 48, 72 hours, and the application of PDGF-BB for 8 min showed less proliferation rate of gingival fibroblasts compared to the application of PDGF-BB for 4 min at 24, 48, 72 hours. In conclusion, the application of PDGF-BB for 4 min appeared to be optimal to obtain maximum proliferation of gingival fibroblasts using demineralized dentin surface as primary applicaton site of PDGF-BB.

      • Studies on the Anticoagulation Process (Fibrinolysis) by Urokinase I. Purification and Characterization of Urokinase from Human Urine

        박진우,이현재,김수자,Park, Jin-Woo,Lee, Hyun-Jae,Kim, Soo-Ja 생화학분자생물학회 1980 한국생화학회지 Vol.13 No.3

        Urokinase는 비활성 혈장단백질인 프라스민 전구체를 활성화시키는 효소로서, 이 효소반응의 산물인 프라스민이 혈류의 응혈요소인 피브린 가수분해 과정에 참여될 수 있음으로 이 효소, 즉 Urokinase가 지닌 특성을 연주 검토하였다. 효소 시료의 정제는 ECTEOLA 수지에 의한 이온 교환 크로마토그래피 방법과 알지닌 또는 아그마틴을 ligand로 하는 친화성 크로마토그래피 방법을 도입하여 약 60배의 정제도를 얻었으며 이 경우 효소의 비활성도는 약 1,300 units/mg protein 이였다. 효소의 특성 중 특기할만한 사실은 기질 특성으로서, 다른 단백질 분해 효소인 trypsin과 작용은 비슷하나 프라스민 전구체인 프라스민노젠에만 특이하게 작용함을 알았으며 효소활성도의 최적 pH와 최적온도 등에 있어서도 다른 유사단백질 분해효소들과 다름을 알았다. 그 밖의 효소 특성인 기질에 대한 친화력 및 저해제에 의한 결과 등을 토대로 이 효소가 지난 생리적 역할, 즉 피브린 가수분해 조절기능에 대하여도 검토하여 보았다. The activation of plasminogen to an active fibrinolytic enzyme, plasmin, was studied withe urokinase(EC 3.4.26.99). A crude form of urokinase obtained from human urine was purified partially by an ion exchange column of ECTEOLA and further by an affinity column employing arginine or agmatine as a ligand on Sepharose 4B matrix. The specific activity of the final enzyme preparation was about 1,300 units per ㎎ protein based on the caseinolytic assay measuring the rate of casein hydrolysis by plasmin formed. The mode of the enzyme action seems to be similar to that of trypsin in term of the esterase activity, but the enzyme is found to be very specific toward plasminogen as the substrate. Characteristic properties of the enzyme including molecular weight, pH and temderature optimum, and some kinetic properties were studied and compared with that of the other proteolytic enzyme of trypsin and plasmin. In addition, based on its associative properties, the nature and the physiological role in the fibrinolysis were also discussed.

      • KCI등재

        이산화티타늄-발광체 복합소재 제조 및 가시광선 광촉매 반응

        박진우,김정식,Park, Jin-Woo,Kim, Jung-Sik 한국세라믹학회 2008 한국세라믹학회지 Vol.45 No.11

        This paper presents a study on the photocatalytic reaction about the composite particles of $TiO_2$-coated phosphors under visible light irradiation. Nanocrystalline titanium dioxide layers were directly coated on the alkaline earth aluminate phosphor, $CaAl_2O_4:Eu^{2+},\;Nd^{3+}$ particles by an sol-gel processing method. The photocatalytic reaction was analyzed with the degradation of methylene blue (MB) aqueous solution under UV and visible light irradiations. $TiO_2$-coated phosphor powders showed different photocatalytic mechanism, compared with pure $TiO_2$ (P-25, Degussa). Under UV-irradiation, $TiO_2$-coated phosphor powders showed slow photocatalytic reactivity in the early stage and fast in the latter, compared with that of pure $TiO_2$. However, $TiO_2$-coated phosphor powders showed much faster photocatalytic reactivity than that of pure $TiO_2$ under visible irradiation. In addition, the characterizations of the $TiO_2$-coated phosphor powders were conducted by a X-ray diffractometer (XRD), transmission electron microscope (TEM), and energy dispersive spectroscopy (EDS).

      • KCI등재

        Histological Observations on Bone Healing with Bioactive Glass in Horizontal Ridge Augmentation;A Report of Four Cases

        박진우,서조영,Park, Jin-Woo,Suh, Jo-Young The Korean Academy of Periodontoloy 2006 Journal of Periodontal & Implant Science Vol.36 No.3

        임프란트 식립을 필요로 하는 환자의 수평적 치조제 결손의 증대를 위해 골유도재생술과 병용한 bioactive glass (BG) $(Biogran^{(R)})$ 이식의 골재생 양상을 각기 다른 치유기간을 부여한 4명의 환자에서 평가하였다. 6, 8, 10, 18개월의 치유기간 후 임프란트 식립부위에서 조직절편을 채득하여 골재생을 조직계측학적으로 평가하였다. 임프란트 식립을 위한 surgical reentry시 모든 이식부위는 임상적으로 명확한 수평적 치조제 폭경의 증가를 관찰할 수 있었다. 하지만 조직학적 분석결과 BG는 불량한 골전도성을 나타내었다. 6, 8개월의 치유기간후, 이식부위에서 신생골이 거의 관찰되지 않았으며(2.5%이하), 이식부와 기존 골의 경계부위에서 BG particle에 대한 신생골 성장과 결합양상 또는 관찰할 수 없었다. 10개월의 치유기간후 기존 골조직으로부터 성장한 신생골의 BG particle과의 직접적인 접촉양상을 일부 관찰할 수 있었다. 이식부는 13.2%의 광물화된 신생골조직을 보였고, 대부분의 BG particle은 결체조직으로 둘러싸여 있었다. 18개월의 치유기간이 부여된 환자의 조직절편에서 신생골은 이식부의 10.7%를 차지하여 비교적 낮은 신생골 형성양을 나타내었고, 이식부에 존재하는 잔존BG particle은 대부분은 결체조직으로, 일부분에서 광물화된 골조직으로 둘러싸여 있었다. 6, 8, 10, 18개월에서 잔존 BG particle양은 전체 이식부 면적에 대해서 각기 22.3%, 26.5%, 30.7%, 18.7%로 나타났다. 본 증례보고는 비록 한정적인 4명의 환자에서의 조직계측학적 평가결과이지만, 수평적 치조제 결손의 증대를 위해 골유도재생술과 병용한 bioactive glass이식은 불량한 골전도성으로 인해 효과적인 골재생을 위한 이식재로서는 적절하지 않을 수 있음을 나타낸다.

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