유착에 의한 AGS 및 Hep-G2 세포 표면 구조의 변화

박동규 ( D. K. Park ),전훈재 ( H. J. Chun ),박재홍 ( J. H. Park ),박철희 ( C. H. Park ),진윤태 ( Y. T. Jeen ),이홍식 ( H. S. Lee ),이상우 ( S. W. Lee ),엄순호 ( S. H. Um ),최재현 ( J. H. Choi ),김창덕 ( C. D. Kim ),류호상 ( H. S. Ryu 대한소화기학회 2002 대한소화기학회 춘계학술대회 Vol.2002 No.-

<목적> 최근 H. pylori 유착에 의한 세포 표면 구조의 변화에 관한 연구가 시도되어지고 있으나 actin 의 변화여부 및 그 특성에 관해서는 아직 명확히 정립되지 못한 실정이다. Rho GTPase는 세포 표면의 미세돌기인 microvilli, filopodia 및 membrane ruffle의 형성과 관련이 있으며, 최근 AGS 세포에서 H. pylori가 Rac activation에 의하여 membrane ruffle을 형성한다는 것과 Rac

Quadrupole Mass Spectrometer의 기체별 감도 특성

신용현(Y. H. Shin),홍승수(S. S. Hong),이철로(C. R. Lee),임재영(J. Y. Leem),박재홍(J. H. Park),정광화(K. H. Chung) 한국진공학회(ASCT) 1993 Applied Science and Convergence Technology Vol.2 No.1

부분압 분석 장비의 감도 측정 시스템을 설계 제작하였다. 이 시스템을 이용하여 부분압측정기기로 많이 쓰이는 Quadrupole Mass Spectrometer의 N₂, O₂, Ar, CO, H₂에 대한 감도를 10^(-4)~10^(-8)torr 영역에서 측정하여 감도의 압력 의존성을 보고 검출 방식, Emission current, Secondary Electron Multiplier 전압 등이 감도에 미치는 영향도 측정하였다. 실험 결과 기체 종류에 따라서는 물론 사용조건에 따라서도 감도의 절대값 변화 및 압력 의존성 변화가 아주 심한 것을 알 수 있었다. 따라서 Quardrupole Mass Spectrometer를 정량적인 측정에 이용하기 위해서는 이에 대한 감도 측정 및 특성조사가 반드시 선행되어야 한다. The calibration system for partial pressure analyzer was designed and set up. Using this system, the linearity of two commercial Quardrupole Mass Spectrometer and the influence of operating parameter (emission current, detection mode, SEM voltage) on the sensitivity has been investigated over the pressure range of 10^(-4)~10^(-8) torr for N₂, O₂, Ar, CO and H₂ gases. Sensitivities of Quadrupole mass spectrometers were greatly influenced not only by gas species but also by operating parameters. Thus for all the quantitive applications, it is necessary to characterize and calibrate quadrupole mass spectrometer carefully.

고진공 영역의 진공게이지 특성연구

홍승수(S.S. Hong),신용현(Y.H. Shin),임재영(J.Y. Leem),박재홍(J.H. Park),이철로(C.R. Lee),정광화(K.H. Chung) 한국진공학회(ASCT) 1993 Applied Science and Convergence Technology Vol.2 No.1

당 연구실에서 개발 제작된 동적교정방법을 이용한 고진공표준기를 사용하여 10^(-6)~10^(-3) torr 영역에서 가장 널리 쓰이는 spinning rotor gauge와 열음극 전리 진공게이지들의 특성을 상품화되어 사용되는 방법대로 측정하여 조사하여 보았다. 게이지들의 직선성, 단기간(15일)에 행해진 반복측정에서의 scattering을 조사하였고 또한 N₂, CO, He, H₂, O₂, Ar 가스에 대한 감도와 압력을 올릴 때와 내릴 때의 hysteresis를 조사하였다. The characteristics of spinning rotor gauges and hot cathode ionization gauges, most widely used HV to UHV gauges, were studied in the pressure range of 10^(-6)~10^(-3) torr. The linearities and data scatterings in the time period of 15 days were investigated and also the sensitivities and the hysteresis in the up-down measurements in the N₂, CO, He, H₂, O₂, Ar gases.

비태인이 산란계의 면역 반응에 미치는 영향

박재홍,류경선,Park, J.H.,Ryu, K.S. 한국가금학회 2007 한국가금학회지 Vol.34 No.1

This experiment was conducted to determine the effect of betaine on immune response in laying hens. A total of 72 ISA-brown laying hens were divided into four groups of 18 hens each and fed corn-soybean meal based diets with addition of 0, 300, 600 and 1,200 ppm betaine for four weeks. The effect of betaine on splenocyte proliferations with mitogens, concanavalin A(Con A) and pokeweed mitogen(PWM), were assayed after incubation using [3H] thymidine uptake. Proliferations of splenocyte were significantly increased by activation of mitogen Con A or PWM. Mitogen effects of Con A were increased by Con A plus betaine injection(0.1 mM), whereas PWM effects did not affect in PWM plus betaine injection(0.1 mM) in vitro. Splenocyte of laying hens fed betaine tended to proliferate in the presence of PWM, but appeared to be slightly suppressed in the presence of Con A in vivo. Proliferation of splenocytes which were stimulated by Con A or Con A+betaine injection(0.1 mM) were increased in dietary 600 ppm betaine, but inhibited in dietary 1,200 ppm betaine supplementation. Spleen weights and sheep red blood cell(SRBC) titers of hens fed betaine tended to increase compared to those of control, but were not significantly different. These results suggested that betaine could increase splenocyte proliferation in vitro.

Effects of Feeding Betaine on Performance and Blood Hormone in Laying Hens

박재홍,강창원,류경선,Park, J.H.,Kang, C.W.,Ryu, K.S. The Korean Society of Poultry Science 2006 한국가금학회지 Vol.33 No.4

An experiment was conducted to investigate the effects of dietary betaine on egg production, estradiol, progesterone, and melatonin secretion in laying hens. Seventy two ISA-Brown laying hens were placed into individual cage and fed four different levels of betaine (0, 300, 600, 1,200 ppm) in diets for four weeks. Basal diets were mainly mixed with corn-soybean and contained 2,800 kcal/kg ME and 16% CP. Dietary supplementation of betaine showed significantly higher (p<0.05) egg weight and daily eggmass than controls, but did not have consistent influence on the egg production. The serum estradiol was statistically different in betaine treatments compared to that of control (p<0.05). No significant changes in serum progesterone concentrations were observed by the addition of betaine in diets. Serum melatonin concentrations also increased with betaine supplementation in diets (p<0.05). The results of this study indicate that the dietary betaine may contribute to increase daily eggmass, serum estradiol and melatonin concentration in laying hens.

육용종계 사료에 시호(Bupleurum falcatum L.)의 첨가가 생산능력, 혈액의 성상 및 호르몬 분비에 미치는 영향

박재홍,강창원,류경선,Park J.H.,Kang C.W.,Ryu K.S. 한국가금학회 2006 한국가금학회지 Vol.33 No.1

An experiment was conducted to investigate the effects of feeding Bupleurum falcatum L.(BFL) on performance, blood component and hormonal secretion in broiler breeder hens. A total of 800 broiler breeder hens were allotted to four treatments(BFL ; 0, 0.5, 1.0, 2.0%) with four replications for eight weeks. Weight gain was not significantly different between the groups in growing period($20{\sim}24$ weeks), but egg production and egg mass of broiler breeder hens fed BFL were significantly(p<0.05) higher than those of the control groups in laying period($24{\sim}28$ weeks). Dietary BFL did not affect serum triglyceride concentration, whereas serum cholesterol concentration was significantly increased(p<0.05) in 2.0% BFL-fed groups. No statistical differences were found in serum glucose, Ca, P, Mg, total protein, albumin, GGT, AST, ALT and BUN concentrations. However, albumin/globulin ratio was statistically higher in BFL-fed groups compared to the control groups(p<0.05). Serum estrogen concentrations were significantly increased by feeding a diet containing 0.5% BFL, but progesterone were not influenced. Serum IGF-I of BFL groups was greater than that of control groups at 28 weeks(p<0.05). In conclusion, the results suggest that the dietary addition of BFL into broiler breeder diets improved egg production, serum estrogen and IGF-I concentration, and decreased serum cholesterol concentration and albumin/globulin ratio.

사료내 비태인과 단백질 수준이 산란계의 생산 능력, 혈액의 조성, 복강지방 및 간의 아미노산 함량에 미치는 영향

박재홍,박수영,류경선,Park J. H.,Park S. Y.,Ryu K. S. 한국가금학회 2005 한국가금학회지 Vol.32 No.3

An experiment was conducted to investigate the effects of dietary betaine and protein levels on performance, internal e99 Qualify, blood composition, abdominal fat and liver amino acid concentration in laying hens. A total of 432 ISA-Brown laying hens were randomly allotted to four treatments with six replications for twenty few weeks. Treatments were factorially designed with two levels of crude protein(14.5 and $16.5\%$) and two levels of betaine(0 and 600ppm). The egg production was increased by feeding $16.5\%$ protein-fed group compared to that of $14.5\%$ protein-fed groups(P<0.05), but was different from betaine supplementation. Increasing protein and betaine supplementation to diets improved egg weight, egg mass and fred conversion(P<0.05). Eggshell breaking strength, thickness and Haugh unit were not influenced by either dietary betaine or protein levels. Dietary betaine did not affect serum total protein, albumin and uric acid concentration, whereas uric acid concentration was increased in $16.5\%$ protein-fed groups(P<0.05). The deposition of abdominal fat was lower in 600ppm betaine groups compared to those fed on 0ppm betaine groups(P<0.05). Concentration of the most hepatic amino acids were affected by dietary protein levels and betaine supplementation. There were synergistic effect between dietary protein and betaine. These results suggested that dietary betaine can improve the egg weight, mass, feed conversion and decrease the abdominal fat, but increase the hepatic amino acid contents.

사료내 비태인과 에너지 수준이 산란계의 간지방과 콜레스테롤에 미치는 영향

박재홍,박수영,류경선,Park J. H.,Park S. Y.,Ryu K. S. 한국가금학회 2005 한국가금학회지 Vol.32 No.3

Two experiments were conducted to examine the effects of betaine intake on blood and yolk cholesterol, abdominal fat, liver fat, tissue triglyceride(TG) and liver HMG-CoA reductase In laying hens. In Expt. 1, a total of 72 ISA-brown laying hens were individually assigned into four treatments from 18 to 21 weeks old. Com-soybean meal based diet were fed with the addition of 0, 300, 600 and 1,200ppm. In Expt. 2, 72 ISA-brown laying hens were housed into individual cage to evaluate the effect of dietary betaine(0, 600ppm) and energy(ME, 2,800, 2,900kca/kg) from 70 to 74 weeks. Serum total cholesterol, LDL and HDL-cholesterol and TG concentration in blood of hens fed betaine tended to increase compared to those of the control, but were not significantly different. However, betaine supplementation showed a statistically significant decrease in yolk cholesterol(P<0.05). There was no significant difference in abdominal fat among the treatments. Liver fats and 7c of birds 130 betaine was decreased compared with control. Serum total cholesterol, LDL-cholesterol and triglyceride concentration were significantly inc.eased by ffeding a diet containing 600ppm betaine in Expt. 2(P<0.05), but were not influenced by the dietary energy levels. Yolk cholesterol, abdominal fat and HMG-CoA reductase activity were affected neither by dietary energy nor betaine level.

산삼 배양액의 급여가 산란계의 생산 능력 및 계란의 품질에 미치는 영향

박재홍,신오식,류경선,Park J. H.,Shin O. S.,Ryu K. S. 한국가금학회 2005 한국가금학회지 Vol.32 No.4

The wild ginseng culture by-products(WGCB) was added to the laying hens diets. A total of 420 Lohmann Brown laying hens were alloted to five levels of drinking water containing WGCB 0, 0.4, 0.8 1.6, $3.2\%$with seven replications and fed com-soybean meal based diets contained ME 2,800 kcal/kg, CP $16\%$. Laying Performance, egg quality, egg yolk fatty acid and cholesterol concentration were measured. Egg Production and daily e99 mass were significantly lower(p<0.05) in birds fed the WGCB $3.2\%$ compared with no WGCB group, but showed no significant effect in other treatments. Feed efficiency of birds fed $0.4\%$ WGCB showed the lowest of all treatments(p<0.05). Eggshell thickness was increased(p<0.05) by WGCB supplementation, whereas eggshell breaking strength, haugh unit and yolk color index were not affected. No significant change in yolk fatty acids and cholesterol were observed by the addition of WGCB.

대장 원위부 선종의 특성에 따른 진행성 근위부 종양의 존재 위험도 분석 - 단변량 및 다변량 분석 -

박재홍 ( J. H. Park ),전훈재 ( H. J. Chun ),박동규 ( D. K. Park ),박철희 ( C. H. Park ),진윤태 ( Y. T,Jeen ),이홍식 ( H. S. Lee ),이상우 ( S. W. Lee ),엄순호 ( S. H. Um ),최재현 ( J. H. Choi ),김창덕 ( C. D. Kim ),류호상 ( H. S. Ryu 대한소화기학회 2002 대한소화기학회 춘계학술대회 Vol.2002 No.-

<목적> S상 결장 및 직장 선종은 20-40%에서 근위부 대장 선종이 동반된다고 보고되고 있으나 원위부 대장 선종의 특성에 따른 진행성 근위부 종양(이하 APN)의 존재 위험에 대한 연구는 미흡한 실정이다. 본 연구는 원위부 선종의 특성에 따른 근위부 선종 과 APN의 동반 위험도를 분석하고자 하였다. <방법> 1998년부터 2001년 사이 전대장 내시경을 처음으로 시행받은 무증상군 7435명 중 용종의 조직학적 검사에서 선종이나 암으로 진단된 484