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RISS 인기검색어
- 검색키워드
- 저자 : 박자혜 (검색결과 3 건)
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대학생의 낙관성과 진로결정수준 간의 관계 : 문제해결중심 스트레스 대처전략과 진로결정 자기효능감의 매개효과
The purpose of this study was to investigate the mediating effects of Problem solving Coping Strategy and Career Decision-making Self-efficacy on the relation between Optimism of College Students and Career Decision-making Level. For that purpose, a survey was taken with 284 college students in Busan, Ulsan, Gyeongnam and Jeonnam. Collected data were analyzed with the structural equation model. As a result, the measuring model turned out to fit with the data. The research findings were as follows: first, Optimism had significant positive correlations with Career Decision-making Level, Problem solving Coping Strategy, and Career Decision-making Self-efficacy. Optimism had significant positve correlations with Career Decision-making Level, Problem solving Coping Strategy, and Career Decision-making Self-efficacy. Second, as for the effects of Optimism on Career Decision-making Level, Problem solving Coping Strategy had no direct effects on Career Decision-making Level, but Career Decision-making Self-efficacy has direct influence on it. Third, Problem Solving Coping Strategy was partially correlated with Optimism and Career Decision-Making Self-efficacy. In addition, Problem Solving Coping Strategy and Career Decision-Making Self-efficacy showed the fractional relationship with Optimism and Career Decision-making Level. On the other hand, the perfect association was confirmed between Problem Solving Coping Strategy and Career Decision-Making Self-efficacy. Last, Problem solving Coping Strategy and Career Decision-making Self-efficacy has significant mediating effects on relations Optimism and Career Decision-making Level. Based on those findings, the study discussed their implications for counseling practice and suggestions for follow-up study.
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박자혜 경북대학교 의학전문대학원 2010 국내석사
Proteomics technology based on mass spectrometry (MS) accelerated the identification of proteins and highly affected on both basic science such as cell signaling and applied science such as drug discovery. More recently, it has been considered that enrichment steps of samples from cell cultures and body fluids are important before MS analysis. In this study, Adenosine-5'-triphosphate (ATP) affinity chromatography been used and evaluated as an enrichment strategy. ATPs are main energy sources used as substrates in energy metabolism and signaling via various kinases and metablic enzymes. Especially, proteins containing ATP binding pocket, such as protein kinase, play a very important role in cell signaling and metabolism. After ATP affinity chromatography, the eluent from resin was analyzed by LC MS/MS-based proteomics technology. As a result, we could identify 68 of ATP-binding proteins, 11 of ATPase activity proteins and 10 of ligase activity proteins among 280 DAVID IDs from mouse myotubes C2C12 cells. Furthermore we applied this method to insulin resistance state in C2C12 cells by means of treatment of O-GlcNAcase inhibitor, PUGNAc for elevating O-GlcNAc level. Among many proteins changed in protein expression, ubiquitin-activating enzyme E1, E2, proteasome subunit 20S, cullin-associated nedd8 -dissociated protein 1, EZRIN were over-expressed whereas nucleoside diphosphate kinase B was down-expressed by PUGNAc. Theses five proteins were confirmed by western blot analysis, respectively. These results suggested that ubiquitin-proteasome systems in insulin resistance are activated by overexpression of E1 and E2 enzymes as well as one of proteasome subunit, 20S. In this study, we found that the ATP affinity chromatography could be one of enrichment method for proteomics analysis and that proteins related to ubiqutin-proteasome pathway could be an alternative target point to understand insulin resistance. 질량분석기에 기반을 둔 프로테오믹스 기법은 단백질 동정 뿐만 아니라 신호전달과 같은 기초 과학이나 신약개발과 같은 응용과학 분야에 많은 기여를 하고 있다. 최근에는 세포 배양이나 체액 등의 샘플을 질량분석기로의 분석하기 이전에 적절한 분리·정제 기법을 적용하는 것이 매우 중요해졌다. 본 연구에서는 아데노신삼인산(ATP) 친화 크로마토그래피 기법을 이용하여 분리·정제를 실시하였다. ATP는 많은 단백질 인산화 효소와 대사 효소들을 통해 에너지 대사나 신호전달에 있어 중요한 에너지원이다. 특히, ATP 결합 구조를 가지는 단백질 인산화 효소와 같은 단백질들은 세포 신호 전달이나 물질대사에 매우 중요한 역할을 한다. ATP 친화 크로마토그래피 후 레진으로부터 용출된 단백질들을 LC MS/MS를 이용한 프로테오믹스 기술을 이용하여 분석하였다. 그 결과, C2C12 mouse myotubes로 부터 280개의 DAVID ID를 가지는 단백질로 부터 68개의 ATP-결합 단백질과 11개의 ATPase 활성을 가지는 단백질, 10개의 lagase 활성을 가지는 단백질을 동정할 수 있었다. 뿐만 아니라 이 방법을 O-GlcNAcase 억제제인 PUGNAc를 처리하여 O-GlcNAc 정도를 증가시켜 인슐린 저항성 상태를 유도한 C2C12 세포에도 적용하였다. Label-free 정량 결과 인슐린 저항성 상태에서 발현의 변화를 보이는 단백질들 중에서 ubiquitin-activating enzyme E1, ubiquitin-conjugating enzyme E2, proteasome subunit 20S, cullin-associated dedd8-dissociated protein 1, EZRIN은 PUGNAc에 의해 증가하였으며 Nucleoside diphosphate kinase B는 감소하였다. 이 다섯 개의 단백질은 western blot결과와도 일치 하였다. 이 결과를 바탕으로 인슐린 저항성 상태에서 ubiquitin-proteasome 시스템이 E1과 E2 효소 뿐만 아니라 proteasome subunit 20S의 증가로 인해 활성화 된다는 것을 알 수 있었다. 본 연구에서는 프로테오믹스 분석 이전에 적용할 분리·정제 기법으로 ATP 친화 크로마토그래피 기법이 적절하게 사용될 수 있으며, ubiquitin-proteasome 신호전달 과정에 관여하는 단백질들이 인슐린 저항성 과정을 이해하는데 타깃 포인트가 될 수 있음을 알 수 있었다.
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