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한외여과된 돈혈청으로부터 DEAE - Sephacel Chromatography 에 의한 면역단백질의 분리
전기홍,유익종,박우문,이무하 ( K . H . Jeon,I . J . Yoo,W . M . Park,M . H . Lee ) 한국축산학회 1997 한국축산학회지 Vol.39 No.1
Concentrated porcine serum with ultrafiltration 30KDa was purified with DEAF-Sephacel column chromatography. Buffer solution with pH 7 to 9 and buffer concentration with 0.01M to 0.05M, and NaCl gradient with 0M∼0.5M, 0M∼0.8M and 0M∼1.0M were used respectively for chromatographic purification. Optimal conditions were at pH 8.0, buffer conc. 0.03M and salt conc, 0M∼0.5M with immunoprotein concentration 82% and production yield 43%. Purified immunoprotein was identified in the molecular weight 50KDa and 15KDa by electrophoresis.
발효식품에서 분리한 젖산균이 발효소시지의 저장중 이화학적 특성에 미치는 영향
박우문,최원희,유익종,김왕준,지중룡,정동효 ( W . M . Park,W . H . Choi,I . J . Yoo,W . J . Kim,J . R . Ji,D . H . Chung ) 한국축산학회 1997 한국축산학회지 Vol.39 No.1
The effects of Lactobacillus plantarum, which produce bacteriocin, isolated from fermented foods (NFS #6-6, fermented sausage: S #3, kajami sikhae) on the physico-chemical properties of fermented sausages were compared with commercial starter culture, SL during storage at 24℃ and 35℃. Sausages treated with NFS #6-6 and S #3 had higher a value than SL at 24℃. Saturated fatty acid (SFA) were increased but unsaturated fatty acid (USFA) were decreased after 4 weeks storage and that ratio were high as the storage temperature were increased, regardless of treated starters. Free amino acid contents were decreased but glutamic acid, lysine and aspartic acid were detected in large amount as compared with other free amino acids after 4 weeks storage, regardless of storage temperatures and treated starters. TBA values were 0.24∼0.27 ㎎/㎏ at 24℃ and 0.49∼0.52 ㎎/㎏ at 35℃ after 4 weeks storage and did not show any difference among samples.
발효식품에서 분리한 젖산균이 발효소시지의 저장중 미생물의 특성에 미치는 영향
박우문,최원희,유익종,김왕준,전기홍,정동효 ( W . M . Park,W . H . Choi,I . J . Yoo,W . J . Kim,K . H . Jeon,D . H . Chung ) 한국축산학회 1997 한국축산학회지 Vol.39 No.1
The effects of Lactobacillus plantarum, which produce bacteriocin, isolated from fermented foods(NFS #6-6, fermented sausage: S #3, kajami sikhae) on the property of fermented sausages were compared with commercial starter culture, SL by measurement of pH, Aw and microbiological characteristics during storage at 24℃ and 35℃. There was slight increase in pH for all treatment and storage at 35℃ lowered pH than storage at 24℃. Lactobacilli count of S #3 and NFS #6-6 treated samples stored at 24℃ maintained 10^7 CFU/g during storage but that of SL treated samples reduced to 10^5 CFU/g after 4 week storage. Lactobacilli count of samples stored at 35℃ were reduced during storage. Staphylococci after 4 week storage at 24℃ were maintained as 10⁴ CFU/g for NFS #6-6, 10³ CFU/g for S #3 and 10² CFU/g for SL treated samples. Staphylococci of samples storage at 35℃ were reduced faster than 24℃ storage. Listeria were effectively inhibited in samples with NFS #6-6 and extinguished after 4 week storage in all treatment.