고추 소포자 배양시 전처리 배지와 생장조절물질이 소포자배 생산에 미치는 영향

박은준,유은지,김리나,김문자 한국육종학회 2007 한국육종학회 학술발표회 발표요지 Vol.39 No.-

국내 간호사의 직장 내 괴롭힘 경험: 질적 메타 종합연구

박은준,강현욱,고지운 한국간호교육학회 2023 한국간호교육학회지 Vol.29 No.4

Purpose: This study aimed to conduct a qualitative synthesis of workplace bullying experiences among nurses in Republic of Korea. Methods: Following the PRISMA guideline, a literature search was conducted using seven domestic and three international databases. Studies published in Korean or English from inception to December 31, 2022 were included. A meta-aggregation approach suggested by the Joanna Briggs Institute was used to synthesize the research findings. Results: Fourteen studies were included in this review. As a result of a data analysis of the selected studies, 199 subthemes and supporting illustrations were identified and grouped into 36 related categories. Based on the subthemes and categories, five synthesized findings were developed: (1) the individual and organizational causes of workplace bullying; (2) the various types of physical violence and psychological harassment; (3) the negative impact of workplace bullying and its effect on self-growth; (4) active and passive coping efforts in dealing with bullying; and (5) strategies for preventing bullying incidents. Conclusion: Based on the synthesized findings, four recommendations were made: (1) improving the challenging working conditions for nurses; (2) enhancing educational programs for new nursing graduates; and (3) promoting proactive responses from nursing managers in conjunction with an expansion of resilience training for nursing students. Finally, to address the issue of workplace bullying, (4) multi-center and multi-level research involving nursing organizations needs to be conducted.

의료급여 관리사의 실무 경험을 통해서 본 사례관리 과정

박은준,김춘미 한국간호과학회 2008 Journal of Korean Academy of Nursing Vol.38 No.6

The purpose of this study was to develop a substantive theory of case management (CM) practice by investigating the experience of nurse case managers caring for Medical Aid enrollees in Korea. Methods: A total of 12 nurses were interviewed regarding their own experience in CM practice. Data were recorded and analyzed using grounded theory. Results: Empowerment was the core category of CM for Medical Aid enrollees. The case managers engaged in five phases as follows, phase of inquiring in advance, building a relationship with the client, giving the client critical mind, facilitating positive changes in the client’s use of healthcare services, and maintaining relationship bonds. These phases moved gradually and were circular if necessary. Also, they were accelerated or slowed depending on factors including clients’ characteristics, case managers’ competency level, families’ support level, and availability of community resources. Conclusion: This study helps understand what CM practice is and how nurses are performing this innovative CM role. It is recommended that nurse leaders and policy makers integrate empowerment as a core category and the five critical CM phases into future CM programs.

어류 경구백신 현황과 전망

박은준,김미나,박주영,차재호,정화지,Park, Eun-Joon,Kim, Mi-Na,Park, Ju-Young,Cha, Jae-Ho,Chung, Hwa-Jee 한국식물생명공학회 2010 식물생명공학회지 Vol.37 No.3

As the capture fishing industry has declined, the aquaculture industry has become an important source of seafood. With this tendency all fish farming will be performed by large-scale farms where the fish are cultivated in much high density and as a result the incidence of infectious diseases increases. Therefore, vaccination has become an increasingly important part of aquaculture as a cost effective method of controlling various diseases. The early fish vaccines were the formalin inactivated bacteria or virus cultures, which were administered by either immersion or injection. Recombinant DNA biotechnology allowed us to develop orally administrated DNA and recombinant vaccines. In terms of the manufacturing process and cost, Lemna and Spirodela is the most efficient and reliable plant expression system for the production of edible vaccine.

사례관리 만족도 측정에 관한 고찰

박은준 대한간호학회 간호행정학회 2007 간호행정학회지 Vol.13 No.3

연구 목적: 사례 관리(CM) 환자 만족도 측정에 관한 연구 결과를 고찰하여 사례관리 실무에서 활용할 수 있는 근거를 제공하고자 한다. 연구 방법: MedLine, CINAHL에서 주요용어로 case management, care management, 주제어로 patient satisfaction, client satisfaction을 이용해 문헌 검색을 실시하였다. 연구 결과: 만족도에 대한 개념은 관련 연구가 매우 부족하며, 서로 다른 이론적 접근을 하고 있으므로 그 측정에 어려움이 있다. 신뢰할 만한 만족도 측정을 위해서는 설문 문항을 서술하는 여러 가지 방법들, 측정 척도의 종류, 만족도에 영향을 미치는 혼란변수들을 주의 깊게 고려하여야 한다. 만족도는 사례관리 연구에서 흔히 측정되는 성과이지만 대부분의 관련 연구에서 사례관리의 특징이 반영된 측정 도구를 사용하지 않고 있었다. 따라서 본 논문은 다섯 개의 사례관리 만족도 측정 도구를 제시하고 그 특징을 소개하였다. 또한, 간호성과분류체계(The Nursing Outcomes Classification)에 제시된 만족도 측정항목들 중 사례관리와 관련이 높은 약 40%의 항목들을 발견하였다. 결론: 사례관리 만족도 측정 도구가 매우 부족하므로 표준 사례관리 측정도구 개발이 시급하다. 한편 사례관리사들은 만족도 측정에 대한 이해를 높이고 기존 연구 결과를 활용함으로써 보다 정확한 사례관리 만족도 평가가 이뤄지도록 노력해야 한다.

고추의 소포자 배양 시 전처리 배지, 새 배지의 첨가, 및 배양 용기의 크기가 배의 생산에 미치는 영향

박은준,김진애,김문자 한국식물생명공학회 2009 식물생명공학회지 Vol.36 No.2

The influences of pretreatment medium, the addition of fresh medium, and the size of culture plate on the production of embryos were investigated in isolated microspore culture of hot pepper (Capsicum annuum L.). Among the media used for heat shock pretreatment (32±1℃), high frequency embryo production was obtained when the sucrose- starvation medium A was used. On the other hand, neither 0.37 M mannitol solution nor NLNS medium supplemented with sucrose was not efficient for embryo production. The addition of culture medium to pretreatment media considerably decreased the embryo production even though embryo development proceeded further. The embryo production was not improved by the addition of fresh medium after 2 or 3 weeks from starting culture. Increase in the size of the culture plate from 3.5×1.0 cm to 6.0×1.5 cm improved embryo quality. These results will provide valuable information for developing an efficient microspore culture system of hot pepper for high frequency embryo production. 고추의 소포자 배양 시 전처리 배지, 배양 후 새 배지의 첨가, 및 배양 용기의 크기가 배의 발생 및 발달에 미치는 영향을 조사하 였다. 고온 전처리 (32±1℃) 시 사용된 배지 중 배의 생산에 적합한 것은 당-기아배지 A이었으며, 0.37 M mannitol 용액이나 sucrose가 첨가된 NLNS배지는 배의 생산에 비효율적이었다. 전처리 배지인 당-기아배지 A와 0.37 M mannitol 용액에 배양배지를 첨가하는 경 우 배의 발달 정도는 증가하였으나 배의 생산은 크게 감소하였다. 배양 2, 3 주 후의 새 배지 첨가는 배의 생산에 효과가 없었다. 배 양용기는 3.5×1.0 cm 크기의 것 보다는 6.0×1.5 cm 크기의 것을 사 용하는 것이 배의 발달에 효과적이었다. 이상에서와 같은 결과들 은 고추에서 다수의 배를 생산할 수 있는 소포자 배양시스템을 확 립하는데 중요한 기초자료가 될 것이다.

고추의 소포자 배양 시 전처리 후 배지의 교환, 배지의 첨가 및 2층배양 시 하층고체 배지의 양이 배의 생산에 미치는 영향

박은준,이종숙,안동주,김문자 한국식물생명공학회 2010 식물생명공학회지 Vol.37 No.4

The effect of the addition of the fresh medium, volume of under solid medium in double layer culture as well as the medium change after pretreating microspores on the production of embryos in microspore culture of hot pepper (Capsicum annuum L.) has been studied. When cultured after heat pre-treatment, changing pretreatment media with fresh culture media proved to be more effective for embryo production rather than supplementing additional culture media. Heat-pretreating for 3 days turned out more effective for embryo production than pretreating for 1 or 2 days. In the case of anther pretreatment, the addition of fresh medium after culture was not effective for embryo production. In pretreating microspores, however, supplementing additional fresh culture media greatly improved embryo yield and quality. The best time point of media addition was 4 days after culture commenced, and the most effective number of times of media addition was one time addition. Moreover, the effective volume of added medium in double layer culture for embryo production was 1.5 ml. The addition of media more than 1.5 ml reduced both embryo yield and quality. Double layer medium was more effective for embryo development than liquid medium. When the volume of under solid medium increased ranging from 3 ml to 7 ml, more cotyledonary embryos were produced in either 5 ml or 7 ml compared to 3 ml, even though the total number of embryos were highest in 3 ml. These results can be used as an important data for establishing an efficient microspore culture system for producing high frequency of normal embryos in hot pepper. 본 연구에서는 고추의 소포자 배양 시 전처리 후 배지의 교환 여부, 배양 후 새 배지의 첨가 및 2층배지 사용시 하층고체배지의 양이 배의 생산에 미치는 영향을조사하였다. 고온 전처리 후 배양배지를 첨가하여 배양하는 것보다 사용한 전처리 배지를 새 배양 배지로 교환하여 배양 하는 것이 배의 생산에 효과적 이었으며,고온전처리 기간은 1일이나 2일에 비해 3일이 효과적 이었다. 배양 후 새 배지의 첨가는 약전처리 시에는 효과가 없었으나 소포자 전처리 시에는 배의 유기와 발달모두 크게 향상되었다. 새 배지의 첨가 시기는 배양 4일후가, 첨가 횟수는 1회가 배의 생산에 가장 효과적이었다. 한편 2층배지 사용 시 첨가하는 새 배지의 양은 1.5 ml이효과적이었으며 이보다 많은 양을 첨가하는 경우 배의발생과 발달 모두 저하되었다. 액체배지 사용 시에 비해 2층배지 사용 시 배의 발달이 좋았다. 또 2층배지 사용 시 하층고체배지의 양이 3 ml 일 때 보다는 5 ml이나 7 ml일 때 배의 발생은 감소하였으나 배의 질이 향상되었다. 이와 같은 결과들은 고추에서 다수의 정상자엽배를 생산 할 수 있는 소포자 배양시스템을 확립하는데 중요한 기초자료가 될 것이다.

임상간호사에서 의료급여관리사로의 역할 전이 과정 : ‘척박한 땅에 뿌리내리기’

박은준,김춘미,임승주 한국간호과학회 2012 Journal of Korean Academy of Nursing Vol.42 No.2

Purpose: The purpose of this study was to explain the role transition process to nurse case managers (NCMs) for Medical-aid beneficiaries in Korea. Methods: Fourteen NCMs were interviewed regarding their experiences of becoming proficient in the new role of case manger. Data were analyzed through the application of grounded theory. Results: ‘Taking root in a barren land" was the core category explaining the role transition process of NCMs. They engaged in four stages: launching, trial and error, proficiency, and wait-and-see stages. NCMs showed not only fear but also passion for case management practice. Despite their passion and effort, NCMs went through a period of trial and error. After becoming skilled, NCMs went through a stage of wait-and-see often because of job insecurity related to temporary position or few opportunities for promotion. Factors influencing NCMs" role transition process included their understanding of client characteristics, belief in case management, and support from their colleagues and families. Conclusion: NCMs experience many challenges in the process of becoming proficient NCMs. To help with their role transition, there is a need for education programs, preceptorship programs, research on their roles and functions, and regulation for securing NCMs" employment and career stability.

연명의료 관련 신문 기사의 텍스트네트워크분석

박은준,안대웅,박찬숙 한국지역사회간호학회 2018 지역사회간호학회지 Vol.29 No.2

Purpose: This study tried to understand discourses of life-sustaining treatments in general daily and healthcare newspapers. Methods: A text-network analysis was conducted using the NetMiner program. Firstly, 572 articles from 11 daily newspapers and 258 articles from 8 healthcare newspapers were collected, which were published from August 2013 to October 2016. Secondly, keywords (semantic morphemes) were extracted from the articles and rearranged by removing stop-words, refining similar words, excluding non-relevant words, and defining meaningful phrases. Finally, co-occurrence matrices of the keywords with a frequency of 30 times or higher were developed and statistical measures―indices of degree and betweenness centrality, ego-networks, and clustering―were obtained. Results: In the general daily and healthcare newspapers, the top eight core keywords were common: “patients,” “death,” “LST (life-sustaining treatments),” “hospice palliative care,” “hospitals,” “family,” “opinion,” and “withdrawal.” There were also common subtopics shared by the general daily and healthcare newspapers: withdrawal of LST, hospice palliative care, National Bioethics Review Committee, and self-determination and proxy decision of patients and family. Additionally, the general daily newspapers included diverse social interest or events like well-dying, euthanasia, and the death of farmer Baek Nam-ki, whereas the healthcare newspapers discussed problems of the relevant laws, and insufficient infrastructure and low reimbursement for hospice-palliative care. Conclusion: The discourse that withdrawal of futile LST should be allowed according to the patient’s will was consistent in the newspapers. Given that newspaper articles influence knowledge and attitudes of the public, RNs are recommended to participate actively in public communication on LST.

어류 경구백신 현황과 전망

박은준,김미나,박주영,차재호,정화지 한국식물생명공학회 2010 JOURNAL OF PLANT BIOTECHNOLOGY Vol.37 No.3

As the capture fishing industry has declined, the aquaculture industry has become an important source of seafood. With this tendency all fish farming will be performed by large-scale farms where the fish are cultivated in much high density and as a result the incidence of infectious diseases increases. Therefore, vaccination has become an increasingly important part of aquaculture as a cost effective method of controlling various diseases. The early fish vaccines were the formalin inactivated bacteria or virus cultures,which were administered by either immersion or injection. Recombinant DNA biotechnology allowed us to develop orally administrated DNA and ecombinant vaccines. In terms of the manufacturing process and cost,Lemna and Spirodela is the most efficient and reliable plant expression system for the production of edible vaccine.