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RISS 인기검색어
- 검색키워드
- 저자 : 박원철(Won Chull Bak) (검색결과 47 건)
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박원철,나용준,Bak Won Chull,La Yong Joon The Korean Society of Plant Pathology 1985 Plant Pathology Journal Vol.1 No.1
마이코플라스마에 감염된 대추나무, 뽕나무 및 일일초의 조직절편을 형광염색소인 DAPI(4'-6-diamidino-2-phenylindole$\cdot$2HCl), aniline blue 그리고 quinacrine(quinacrine mustard dihydrochloride)으로 염색하여 형광현미경하에서 관찰함으로서 이들 형광염색소의 마이코프라스마 감염진단에의 효용가치를 비교 조사하였다. 이병식물의 줄기절편의 절부에서 특이형광반응이 나타났으나 건전식물의 조직절편에서는 특이형광반응이 관찰되지 않음으로써, DAPI, anilinc blue 및 quinacrine 등의 형광염색소에 의한 조직염색법은 대추나무, 뽕나무 및 일일초의 마이코플라스마 감염을 식속 정확하게 진단하는 데 매우 유용한 방법임을 보여주었다. 한펀, 이들 형광염색소 중에서는 DAPI가 가장 효과적이었다. Attempts were made to evaluate the efficacy of three fluorochromes, i.e., DAPI (4'-6-diamidino-2-phenylin-dole-2HCl), aniline blue and quinacrine(quinacrine mustard dihydrochloride) for the detection of mycoplasma infections in jujube (Zizyphus jujuba), mulberry (Mows alba) trees and periwinckle (Catharanthus roseus) plant by fluorescence microscopy. Stem sections from these plants infected with mycoplasma-like organisms (MLO) produced distinct fluorescence in the phloem when stained with DAPI, aniline blue or quinacrine, while fluorescence was absent in the healthy plants. The use of these fluorochromes provided simple and efficient techniques for the diagnosis of MLO infections. IOf the three fluorochromes tested, DAPI was found to be most efficient.
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박원철 ( Won Chull Bak ),박영애 ( Young Ae Park ),이봉훈 ( Bong Hun Lee ),가강현 ( Kang Hyeon Ka ),박지헌 ( Ji Heon Park ) 한국균학회 2013 한국균학회지 Vol.41 No.1
New Shiitake (Lentinula edodes) strain “Chunbaegko” was bred by Di-mon method. Bed-log cultivation of “Chunbaegko” was performed. Fruit-body production of “Chunbaegko” was most at spring and autumn. Optimal temperature of fruit-body formation was 14~22oC Baegwhako, the best quality fruit-body, is produced during spring. The diameter of pileus is ca. 52 mm. The total amount of fruit-body production during 4 years(one generation) was 140 kg/m3 log.
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Berberine Sulphate 를 이용한 형광현미경기법에 의한 수목마이코플라스마검정
박원철(Won Chull Bak),나용준(Yong Joon La) 한국산림과학회 1991 한국산림과학회지 Vol.80 No.2
The efficacy of berberine sulphate, a fluorochrome having binding properties with both DNA and RICA, was investigated for the detection of mycoplasma-like organisms(MLOs) in jujube(Zizyplzus jujuba), paulownia (Paulounia tomentosa), mulberry(Morus alba) trees and periwinkle (Catharantlaus roseus) plant. When examined under fluorescence microscope, berberine sulphate-stained sections of diseased samples showed distinct MLO-specific fluorescent particles in the phloem area, while such fluorescence was absent in the healthy ones. This staining technique was proved to be a very accurate method for the diagnosis of MLO infections in woody and herbaceous plants. Furthermore, the cheap and easy procedure could be used to test a, great number of samples on MLO infections with reliability and rapidity.
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