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허혈성뇌졸중 흰쥐모델에서 가미보양환오탕(加味補陽還五湯)와 주요 구성약재인 황기(黃?), 단삼(丹蔘)의 뇌신경보호효과에 대한 연구
박용기 ( Yong Ki Park ),손혜영 ( Hye Young Son ) 대한본초학회 2010 大韓本草學會誌 Vol.25 No.2
Objectives: In this study, the neuroprotective effects of modified Boyanghwano-Tang (mBHT) and the major medicinal plants, Astragali Radix(AR) and Salviae Miltiorrhizae Radix(SMR) were investigated in transient middle cerebral artery occlusion (tMCAO)-induced ischemic stroke of rats. Methods: mBHT(400㎎/㎏) and AR(154㎎/㎏) or SMR(62㎎/㎏) water extract orally injected in rats after 90 min occlusion of MCA and then allow reperfusion to 24 h. Brain infarction was measured by TTC staining and the expressions of NOS isoforms and apoptotic molecules were determined in ischemic brain by Western blot. Results: The results showed that mBHT has stronger neuropreotective property through inhibitions of the PARP cleaved and caspase-3 activation in ischemic rats, and could reduced infarction volumes comparison of those of AR or SMR, respectively. While, AR extract has an angiogenic property through increasing the expressions of eNOS and VEGF, and SMR extract has a strong anti-inflammatory effects through inhibition of iNOS expression in ischemic brains. Conclusions: These results suggest that mBHT has multifactorial therapeutic advantages through anti-apoptosis, anti-inflammation and angiogenesis for ischemic stroke based on a synergistic combination of ingradients rather than monotherapy.
WHW(R)의 랫드에서의 반복경구투여 독성에 관한 연구
박용기 ( Yong Ki Park ),오태우 ( Tae Woo Oh ),배효상 ( Hyo Sang Bae ),윤철호 ( Cheol Ho Yoon ) 대한본초학회 2010 大韓本草學會誌 Vol.25 No.3
Objectives: We investigated the repeated-dose toxicity of Wenpitang-Hab-Wulingsan(WHW), a Korean traditional medicine prescribed with twelve herbs, which has been used for the treatment of renal disease. Methods: WHW extract prepared by GLP company. WHW was supplemented by gavage at 0, 100, 500 and 1000㎎/㎏/day for 13-week consecutive days. We recorded the clinical signs of toxicity, body weight, organ weights, hematology, gross and histological changes in target organs rats and clinical chemistry analysis for all rats. Results: WHW extract at all doses was shown no mortality or abnormal clinical signs in rats during at the observation period. Furthermore, there was no difference in body weight and food-take consumption, organ weight, gross pathological findings, and urine analysis among the groups of rats treated with different doses of WHW extract. The hematological analysis and clinical blood chemistry data were revealed no toxic effects from WHW-treated rats. Conclusions: The results suggest that WHW extract in rats is a wide margin of safety on a acute toxicity.
우슬(牛膝)의 콜라겐 유도 관절염 생쥐에 대한 개선 효과
박용기 ( Yong Ki Park ),김창수 ( Chang Soo Kim ) 대한본초학회 2010 大韓本草學會誌 Vol.25 No.4
Objectives: The present study was undertaken to determine whether the water extract of Achyranthis Radix, which is the roots of Achyranthes japonica (Achyranthis Radix, AR), is efficacious against collagen-induced arthritis (CIA) in DBA/1J mice. Methods: Mice were immunized with bovine type Ⅱ collagen and orally treated with AR-W (50 and 100㎎/㎏/bw) from days 21 to 42 after immunization. Arthritis was evaluated by arthritic score, histological examination of knee joint and serological markers such as TNF-a, PGE2 and anti-type II collagen (CⅡ)IgG. Results: The results showed that comparing with untreated CIA mice, treated with AR-W significantly suppressed the clinical score and joint tissue pathological damages, reduced the serum levels of TNF-a, PGE2 and anti-CⅡ IgG in CIA-mice. These results suggest that AR-W can effectively alleviate inflammatory response on CIA, and anti-inflammatoy of AR-W can be attributed, at least partially, to the inhibition of inflamamtory mediators, PGE2 and TNF-a, in CIA. Conclusions: This study suggests that AR-W has a therapeutic potential in inflammatory joint diseases such as rheumatoid arthritis.
북강활(北羌活) 물추출물의 항알레르기 효능에 대한 연구
박용기 ( Yong Ki Park ),정진기 ( Jin Ki Jung ),정혜미 ( Hye Mi Jung ),서운교 ( Woon Gyo Seo ) 대한본초학회 2010 大韓本草學會誌 Vol.25 No.3
Objectives: Allergy is an immune dysfunction caused by degranulation from mast cells in the early phase of allergic disease including allergic rhinitis (AR). The purpose of this study was to investigate the effects of Osterici Radix, roots of Ostericum koreanum Maximowicz in human mast cells and experimental allergic animal models. Methods: The anti-allergic effect of Osterici Radix water extract (NK-W) was investigated in human mast cell line, HMC-1 cells, and compound 48/80-induced systemic anaphylactic response in rats and ovalbumin (OVA)-induced AR in mice. Animals were orally administrated with NK-W (10 and 50㎎/㎏) or anti-histamine drug, dosodium cromoglycate (50㎎/㎏), and then intraperitoneally injected with compound 48/80 (8㎎/㎏) or sensitized with 0.1% OVA into nasal. Animals were observed plasma histamine and histological changes of nasal mucosa. Also, mast cell degranulation and histamine production were determined in compound 48/80-stimulated HMC-1 cells. Results: NK-W inhibited compound 48/80-induced degranulation of mast cells and histamine releasing in HMC-1 cells. NK-W decreased mortality and serum histamine releasing in compound 48/80-induced anaphylatic rats in a dose-dependently manner. NK-W also inhibited serum histamine levels in OVA-induced AR mice and improved abnormal histological changes such as expansion of grandular cells and hypertrophy of epithelium in the nasal mucosa. These results indicate that Osterici Radix water extract suppress allergic response through downregulation of mast cell activation. Conclusions: This study suggests that a therapeutic potential of Osterici Radix as a source of anti-allergic agents for use in a number of allergic diseases.