고전적 Kaposi 육종 , 피부 전구암 및 피부암에서 Human Herpesvirus 8 DNA Sequence 검출

박영홍,이상석,최성문,성호석,왕한영,박성욱 대한피부과학회 2000 大韓皮膚科學會誌 Vol.38 No.7

Background: Human herpesvirus 8 (HHV8) has been detected in all forms of Kaposi's sarcoma (KS). The role of HHV 8 in dermatologic diseases other than KS is controversial. Some studies based on polymerase chain reaction (PCR) findings suggest an association between HHV8 and epithelial tumors of the skin. Objective: To assess the presence of HHV8 sequences in classic KS and some premalignant and malignant cutaneous epithelial tumors by nested PCR and find out any variations in HHV8 DNA by direct sequencing. Methods: In total 71 sample tissues were obtained from Korean patients: 5 classic KS, 12 Bowen's disease (BD), 19 actinic keratosis, 14 squamous cell carcinomas and 21 basal cell carcinomas. The first and nested PCR were performed to amplify the fragments of HHV8 open read frame (ORF) 26 and ORF 75 which were directly sequenced by dideoxy termination methods. Results: HHV8 DNA sequences were found in 3 of 5 classic KS (60%) and in 1 of 12 BD. Other epithelial tumors were negative for HHV8. On sequencing PCR products, substitutions at 1033 C→T, 1086 C→T, and 1139 A→C were detected in ORF 26 of all 4 positive PCR products. In 2 of 3 KS positive for ORF 26, substitution was detected at 237 G→A in ORF 75. Conclusion: All of our Korean samples were included in the Zong's C strain. This study suggests that C strain is predominant in classic KS in Korea. But it is rare in non-KS cutaneous epithelial tumors. Studies of the length difference in the HHV8 genome should follow.

연속적 시뮬레이션 모델을 이용한 대학정보화의 도입효과 분석

박영홍,김경수 대한산업공학회 2000 대한산업공학회 추계학술대회논문집 Vol.2000 No.-

다중요소 상호간의 연관성과 연속적 시뮬레이션 기법을 이용한 생산성 측정방법에 관한 연구

박영홍 한국시뮬레이션학회 2002 한국시뮬레이션학회 논문지 Vol.11 No.1

This article presents the results of research to develop a descriptive model of firm-level productivity that will allow a myriad of factor interactions to be directly accounted for. The model is a linked set of equations that attempt to capture how changes in one-factor influences the level of another factor. and ultimately bottom-line performance. The model is coded in SIMAN. It is used to determine the best use of an infusion of funds should they go for additional automation, or training etc. An application of the model to U.S. industry is presented based on parameter values obtained through a national survey.

확률적 시뮬레이션을 이용한 공장자동화의 도입 효과분석

박영홍 한국정보전략학회 1999 추계공동학술대회 논문집 Vol.- No.01

우리의 생산현장은 정보화 및 자동화 혁명을 통해 큰 변혁을 맞이하고 있다. 첨단의 생산시설과 최적의 생산방법을 통한 품질의 향상 및 생산비의 절감이 기업의 성패를 좌우할 수 있다는 전제아래 경쟁기업을 능가하는 생산성제고가 무한경쟁시대의 기업 최고의 목표가 되고 있다. 세계화, 국제화, 정보화 추세 속에서 소비자들의 소비 욕구는 점점 다양해지고 세분화되며 고객은 정확한 상품 정보와 신속한 제품의 인도를 요구하고 있다. 따라서 기업은 생존을 위한 전략으로 공장 자동화 시스템을 도입하여 제품의 질적 향상을 도모하면서 최적 생산을 통해 다양한 고객의 욕구에 대응하고 있다. 본 연구는 몬테칼로법에 기초한 확률적 접근방법을 통한 연속적 시뮬레이션 모델을 이용하여 기업이 공장자동화시스템을 도입하기 전, 그 효과를 사전에 측정.평가함으로써 합리적으로 도입방법. 도입규모. 도입내용 등을 결정할 수 있는 방법을 제시한다. 즉 시뮬레이션 모델을 통해 기업이 공장자동화를 추진함에 있어 이에 수반되는 비용과 이로 인한 효과를 생산성 향상 측면에서 분석하여 최적의 공장자동화 도입수준을 결정하고 자동화에 대한 투자효율을 극대화시키기 위한 하나의 척도를 제공하게 된다. This paper forcuses on the measurement of increased work efficiency expected from the factory automation through random interactions of the organizational behavioral factors whose attributes can be changed with the implementation of the factory automations. Specifically the work reported here is concerned with modeling and analyzing the random interrelationships among the organizational behavioral factors which factory automation will have impact on throughout the time horizon of its implementation in terms of productivity. In addition, it is also concerned with developing a stochastic continuous simulation model to be used to assess the impact of factory automations.

갑상선 세포주에서 방사선 조사에 따른 RET 암유전자에 재배열에 관한 연구

박영홍,손우현,김상효 인제대학교 1998 仁濟醫學 Vol.19 No.2

본 연구에서는 실험적인 X-선 조사에 의한 RET 재배열의 유발 가능성을 알아보고 갑상선암 조직에서도 RET 재배열을 검출함으로서 방사선 조사에 의한 RET 재배열과 갑상선암 발생의 상관 관계를 확인하고자 하였다. RT-PCR과 직접 염기서열 결정의 방법으로 X-선을 조사받은 세포주 TT와 SW759와 유두선종 갑상선암 조직 4예를 대상으로 RET의 재배열을 분석하였다. TT와 SW759 세포주에 10-100 Gy의 X-선을 조사한 결과 PTC2-RET 또는 D10S170-RET 재배열이 발생하였으나 URF-RET 재배열은 전혀 발생하지 않았다. 그리고 4예의 갑상선암 조직에서도 D10S170-RET와 PTC2-RET 재배열은 관찰되었으나 URF-RET 재배열은 전혀 관찰되지 않았다. 세포주와 갑상선암 조직에서 관찰된 재배열의 직접 염기서열 결과 모두 동일한 1가지 염기배열만 나타났다. 따라서 갑상선 세포주에서 X-선 조사에 의해 유발되는 RET 유전자의 재배열은 D10S170 및 PTC2와 밀접한 관련을 가지는 것으로 보이며, 이는 갑상선암 조직에서의 실험에서도 이를 부분적으로 확인된다. 이러한 결과로 볼 때 원폭 생존자들 중에서의 암을 포함하여 X-선 조사로 야기된 일부 thyroid cancer들은 방사선 노출에 의해 유발된 특정 형태의 RET 암유전자의 재배열을 통해 발생하게 될 것이라는 가설이 타당함을 보여준다. Objective: To reveal the inducibility and incidence of RET rearrangement by in vitro X-irradiation and to detect RET rearrangement from papillary thyroid cancer Method and Materials: Reverse transcription -polymerase chain reaction and direct sequencing technique were carried out to 0, 10, 50 and 100 Gy X-irradiated TT and SW759 cell lines originated from thyroid cancer. And four samples of papillary thyroid cancer were also analysed. Results: D10S170-RET and PTC2-RET rearrangements were detected in TT and SW759 cell line irradlated at 10 Gy or over, but URF-RET rearrangement not at all. And analysis of papillary thyroid cancers showed D10S170-RET and PTC2-RET rearrangement but not URF-RET rearrangement. D10S170-RET and PTC2-RET rearrangements from cell lines and thyroid cancer tissues had the same sequence, respectively. Conclusion: These results suggest that rearrangement of RET gene of thyroid cell by X-ray irradiation is closely related with D10S170 or PTC2 gene and that RET rearrangement may be critical to thyroid tumorigenesis.

Myoclonic Epilepsy with Ragged-Red Fibers(MERRF) 증후군 환자의 미토콘드리아 tRNALys 유전자에서 점돌연변이의 검출

박영홍,예성수,장원희,박기호 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.5

The author reports a case of myoclonal epilepsy with ragged red fibers(MERRF) syndrome in a patient with confirmed mitochondrial DNA mutation. Diagnosis was made on the basis of the clinical finding, but pathologic finding of muscle tissue didn't reveal the ragged red fiber. The mismatched primer-PCR followed by Nae-IRFLP analysis showed a point mutation from A to G at the 8344th nucleotide located in the mitochondrial tRNALysgene and the heteroplasmy containing wild type and mutant type band. The present report supports the notation that the mitochondrial DNA point mutation at the 8344th nucleotide position is the most common cause of MERRF syndrome. This is the first report of MERRF syndrome analyzed biochemically in Korea and it is necessary to get more detection of MERRF syndrome and the construction of characteristics of pedigree.

소아와 성인의 HBV 관련 신병증에서 HBV pre-S/S DNA 돌연변이의 분석

박영홍,박기호,정우영 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.5

Purpose: The aim of this study was to investigate the mutations of HBV pre-S/S gene in HBV-associated nephropathy patients and to compare the patterns of mutations between child and adult patient, which may suggest the relationship of HBV with these diseases. Method: Seventeen fresh frozen or paraffin-embedded renal tissue samples were obtained from child or adult patients with HBsAg-positive and nephropathy. HBV pre-S/S regions were amplified with PCR and subjected to direct sequencing. Mutations were analyzed at pre-S1, pre-S2 and S gene regions and the difference of pattern was evaluated between adult and child. Results: Seventeen PCR products were direct-sequenced, of which twelve(70.6%) had the mutations at pre-S1 region. Point mutations were detected from all of 12 cases(100.0%) and resulted in amino acid substitutions as Q8OP(CAG->CCG) and L112I(CTA→ATA). Deletion mutations were detected in 2 patients(16.7%) of which one had 27 bp(3149-3175) deletion and the other 2 deletions including l5 bp(2847-2861) and 60 bp(3143-3202) deletions. The mutations at pre-S2 region were detected in 11 cases(64.7%) of which 10(90.9%) had point mutations. Amino acid substitution of T37I due to the point mutation from ACT to ATT was found in 4 cases. Deletion mutations were detected in 3 cases(27.3%) of which one had 66 bp(3203-3213,1-55) deletion which was continuous with the pre-S1 deletion. The other two had 48 bp(nt8-55) and 60 bp(nt3208-3213, 1-54) deletions, respectively. The mutations at S gene were found from all of 17 cases and are exclusively the point mutations but not the deletion mutation. At 'a' determinant(aa124-147), three amino acid substitutions including Ⅰ126N, T131A and G145R were detected. Most of mutations with high frequency and/or amino acid substitution were detected from children, including at Q8OP at pre-S1, T37l at pre-S2 and T131A at S gene. Conclusion: We could find the various mutations including point mutations and deletion mutations at HBV pre-S/S gene in kidney tissue from patients with HBV-associated nephropathy. The comparison of the mutation between the child and adult patients showed the significant difference of mutation pattern.

무증상 B형 간염 보유자에서 한국 야생형 HBV DNA 염기서열의 추론

박영홍,예성수,박기호 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.5

Objective: The aim of this study were to investigate the whole sequence of Korean wild type of HBV including X, C and S regions from asymtomatic HBV carriers and to compare it with other wild types reported previously. Methods and Materials: Sera from 500 healthy person were analysed by HBV-PCR in which positive carriers were subjected to direct sequencing for X, C and S regions. Analysis of regional sequences revealed the tendency of mutations and the sequence of wild type, which were collected and produced the whole sequence. Korean type of HBV was compared with other wild types reported previously. Results: From the PCR analysis, we confirmed 16 carriers(3.2%) all of who had point mutations. The deletion mutations was detected at 4 case of which 2 case had at pre-S1, 1 case at pre-S1 and 1 case at surface gene. Compared with the subtype for Kobayashi, Ono-1, Ono-2, Rho and Sugauchi, sequence variations at 27, 39, 63, 83, 46 sites were found, respectively. Some locations including nt7 at pre-S2, nt705 at S gene and nt2651 upstream to pre-S1 had high frequency of mutations. Conclusion: These observations suggested that Korean wild type of HBV was the most similar to the subtype of Kobayashi and very different from the subtype of Rho which were suspected to the mutants. The high frequency of mutation at some locations including nt7 at pre-S2, nt705 at S gene and nt265l upstream to Pre-S1 might suggest the variants rather than the mutants.

확률적 모의실험을 이용한 정보화 도입효과분석

박영홍 관동대학교 1999 보건환경연구논문집 Vol.5 No.-

Restriction fragment length polymorphism에서 Liquid handling system과 Denaturing High performance liquid chromatography를 이용한 돌연변이의 검출

박영홍 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.2

There had been a great need for the more efficient and rapid method to detect the mutations in DNA. The advance of molecular biology and biotechnology enable the processing and analysis automated including liquid handling system and denature HPLC. The author tried to investigate the efficiency and efficacy of liquid handling system and denaturing HPLC for the detection of the mutations from mitochondrial DNA. Using liquid handling system, author purified the genomic and the PCR-amplified mitochondrial DNA, which were subjected to the treatment of restriction enzyme HaeⅢ, which were analysed by denature HPLC. Restriction fragment length polymorphism showed the four band including 174bp, 242bp, 285bp and 545bp at both cell lines. However 50:50 mixture revealed single-base mutation through the two peaks at 285bp band. These results demonstrated that the combination of both machines was very rapid and efficient for the detection of mutations and introduced the more convenient and reliable data. Especially, large-scale processing using 96 well can make the combination of liquid handling system and denaturing HPLC more valuable.